ButhA.00107.d

Efflux transporter, RND family, MFP subunit

CENTER ID: ButhA.00107.d
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00107.d.A2.GE34799 Mature protein( ButhA.00107.d ) 28 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00107.d.A2.PW35409 Mature protein( ButhA.00107.d ) 28 408

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2322
RefSeq: YP_440294.1
UniProt: Q2T3F5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAILRTSRSR IAAATFAVVV IAGLGAFGAI RVNASAPEKS AAPLPEVDVA TVLSKTITDW QSYSGRLEAV EKVDVRPLVS GTIVSVNFKD GALVKKGDVL FVIDPRPYQA EVDRAAAQLA AAQARNGYAQ SDWQRAQRLI GDNAIAKRDY DEKQNAAREA SANLKAAQAA LETARINLGY TRITAPVSGR VSRAEITLGN VVSAGASAAP LTTLVSVSPI YASFDADEQT YLRYIGGAKD GRKVPVELGL ANESGYSRQG VIDSVDNRLD TSSGTIRVRA RFDNADGSLV PGLYARVKVG GSAPHPALLI DDAAINTDQD KKFVFVVDQQ GRVSYREVQL GAQHGNQRVI AGGLAAGDRI VVNGTQRVRP GEQVKPHLVP MTGGDDAAAT PVAGGVQRPQ GAPGNARA
NT Sequence
ATGGCTATCT TGCGTACTTC CCGTTCGCGG ATCGCGGCCG CGACGTTCGC GGTCGTCGTC ATCGCGGGCC TCGGCGCGTT CGGCGCGATC CGCGTGAACG CGAGCGCGCC GGAGAAATCG GCGGCGCCGC TGCCGGAGGT CGACGTCGCG ACCGTGCTGT CGAAGACGAT CACCGACTGG CAGTCCTACT CGGGCCGCCT CGAGGCGGTC GAGAAGGTCG ACGTGCGTCC GCTCGTGTCC GGCACGATCG TGTCGGTGAA CTTCAAGGAC GGCGCGCTCG TGAAGAAGGG TGACGTGCTG TTCGTGATCG ATCCGCGTCC GTATCAGGCC GAGGTCGATC GCGCGGCCGC GCAGCTCGCC GCCGCGCAGG CCCGCAACGG CTACGCGCAG AGCGACTGGC AGCGCGCGCA ACGCCTGATC GGCGACAACG CGATCGCCAA GCGCGACTAC GACGAGAAGC AGAACGCCGC GCGCGAGGCA TCGGCGAACC TGAAGGCGGC GCAGGCCGCG CTCGAGACCG CACGCATCAA CCTCGGCTAC ACGCGGATCA CCGCGCCCGT GTCGGGCCGC GTGTCGCGCG CGGAGATCAC GCTCGGCAAC GTCGTGTCGG CGGGCGCGTC GGCCGCGCCG CTCACGACGC TCGTATCGGT GTCGCCGATC TACGCGTCGT TCGACGCGGA CGAGCAGACG TATCTGCGAT ACATCGGCGG CGCGAAGGAT GGCCGCAAGG TGCCCGTCGA GCTCGGCCTC GCGAACGAGA GCGGCTACTC GCGGCAGGGC GTGATCGATT CGGTCGACAA CCGGCTCGAC ACGTCGTCCG GCACGATCCG CGTGCGCGCG CGCTTCGACA ACGCGGACGG CTCGCTCGTG CCCGGCCTCT ACGCGCGCGT GAAGGTGGGC GGCAGCGCGC CGCATCCGGC GCTTCTCATC GACGACGCGG CGATCAACAC CGATCAGGAC AAGAAGTTCG TGTTCGTCGT CGACCAGCAG GGCCGCGTGT CGTATCGCGA AGTGCAGCTC GGCGCGCAGC ACGGCAATCA GCGCGTGATC GCGGGCGGCC TCGCGGCGGG CGACCGGATC GTCGTGAACG GCACGCAGCG CGTGCGCCCG GGCGAGCAGG TGAAGCCGCA TCTCGTGCCG ATGACGGGCG GCGACGACGC CGCCGCGACG CCGGTGGCGG GCGGCGTGCA GCGTCCGCAG GGCGCGCCGG GCAACGCGCG CGCA
Details for ButhA.00107.d.A2.GE34799
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVNAS APEKSAAPLP EVDVATVLSK TITDWQSYSG RLEAVEKVDV RPLVSGTIVS VNFKDGALVK KGDVLFVIDP RPYQAEVDRA AAQLAAAQAR NGYAQSDWQR AQRLIGDNAI AKRDYDEKQN AAREASANLK AAQAALETAR INLGYTRITA PVSGRVSRAE ITLGNVVSAG ASAAPLTTLV SVSPIYASFD ADEQTYLRYI GGAKDGRKVP VELGLANESG YSRQGVIDSV DNRLDTSSGT IRVRARFDNA DGSLVPGLYA RVKVGGSAPH PALLIDDAAI NTDQDKKFVF VVDQQGRVSY REVQLGAQHG NQRVIAGGLA AGDRIVVNGT QRVRPGEQVK PHLVPMTGGD DAAATPVAGG VQRPQGAPGN AR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggcg cgatccgcgt gaacgcgagc gcgccggaga aatcggcggc gccgctgccg gaggtcgacg tcgcgaccgt gctgtcgaag acgatcaccg actggcagtc ctactcgggc cgcctcgagg cggtcgagaa ggtcgacgtg cgtccgctcg tgtccggcac gatcgtgtcg gtgaacttca aggacggcgc gctcgtgaag aagggtgacg tgctgttcgt gatcgatccg cgtccgtatc aggccgaggt cgatcgcgcg gccgcgcagc tcgccgccgc gcaggcccgc aacggctacg cgcagagcga ctggcagcgc gcgcaacgcc tgatcggcga caacgcgatc gccaagcgcg actacgacga gaagcagaac gccgcgcgcg aggcatcggc gaacctgaag gcggcgcagg ccgcgctcga gaccgcacgc atcaacctcg gctacacgcg gatcaccgcg cccgtgtcgg gccgcgtgtc gcgcgcggag atcacgctcg gcaacgtcgt gtcggcgggc gcgtcggccg cgccgctcac gacgctcgta tcggtgtcgc cgatctacgc gtcgttcgac gcggacgagc agacgtatct gcgatacatc ggcggcgcga aggatggccg caaggtgccc gtcgagctcg gcctcgcgaa cgagagcggc tactcgcggc agggcgtgat cgattcggtc gacaaccggc tcgacacgtc gtccggcacg atccgcgtgc gcgcgcgctt cgacaacgcg gacggctcgc tcgtgcccgg cctctacgcg cgcgtgaagg tgggcggcag cgcgccgcat ccggcgcttc tcatcgacga cgcggcgatc aacaccgatc aggacaagaa gttcgtgttc gtcgtcgacc agcagggccg cgtgtcgtat cgcgaagtgc agctcggcgc gcagcacggc aatcagcgcg tgatcgcggg cggcctcgcg gcgggcgacc ggatcgtcgt gaacggcacg cagcgcgtgc gcccgggcga gcaggtgaag ccgcatctcg tgccgatgac gggcggcgac gacgccgccg cgacgccggt ggcgggcggc gtgcagcgtc cgcagggcgc gccgggcaac gcgcgcgca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVNAS APEKSAAPLP EVDVATVLSK TITDWQSYSG RLEAVEKVDV RPLVSGTIVS VNFKDGALVK KGDVLFVIDP RPYQAEVDRA AAQLAAAQAR NGYAQSDWQR AQRLIGDNAI AKRDYDEKQN AAREASANLK AAQAALETAR INLGYTRITA PVSGRVSRAE ITLGNVVSAG ASAAPLTTLV SVSPIYASFD ADEQTYLRYI GGAKDGRKVP VELGLANESG YSRQGVIDSV DNRLDTSSGT IRVRARFDNA DGSLVPGLYA RVKVGGSAPH PALLIDDAAI NTDQDKKFVF VVDQQGRVSY REVQLGAQHG NQRVIAGGLA AGDRIVVNGT QRVRPGEQVK PHLVPMTGGD DAAATPVAGG VQRPQGAPGN ARA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcgatc cgcgtgaacg cgagcgcgcc ggagaaatcg gcggcgccgc tgccggaggt cgacgtcgcg accgtgctgt cgaagacgat caccgactgg cagtcctact cgggccgcct cgaggcggtc gagaaggtcg acgtgcgtcc gctcgtgtcc ggcacgatcg tgtcggtgaa cttcaaggac ggcgcgctcg tgaagaaggg tgacgtgctg ttcgtgatcg atccgcgtcc gtatcaggcc gaggtcgatc gcgcggccgc gcagctcgcc gccgcgcagg cccgcaacgg ctacgcgcag agcgactggc agcgcgcgca acgcctgatc ggcgacaacg cgatcgccaa gcgcgactac gacgagaagc agaacgccgc gcgcgaggca tcggcgaacc tgaaggcggc gcaggccgcg ctcgagaccg cacgcatcaa cctcggctac acgcggatca ccgcgcccgt gtcgggccgc gtgtcgcgcg cggagatcac gctcggcaac gtcgtgtcgg cgggcgcgtc ggccgcgccg ctcacgacgc tcgtatcggt gtcgccgatc tacgcgtcgt tcgacgcgga cgagcagacg tatctgcgat acatcggcgg cgcgaaggat ggccgcaagg tgcccgtcga gctcggcctc gcgaacgaga gcggctactc gcggcagggc gtgatcgatt cggtcgacaa ccggctcgac acgtcgtccg gcacgatccg cgtgcgcgcg cgcttcgaca acgcggacgg ctcgctcgtg cccggcctct acgcgcgcgt gaaggtgggc ggcagcgcgc cgcatccggc gcttctcatc gacgacgcgg cgatcaacac cgatcaggac aagaagttcg tgttcgtcgt cgaccagcag ggccgcgtgt cgtatcgcga agtgcagctc ggcgcgcagc acggcaatca gcgcgtgatc gcgggcggcc tcgcggcggg cgaccggatc gtcgtgaacg gcacgcagcg cgtgcgcccg ggcgagcagg tgaagccgca tctcgtgccg atgacgggcg gcgacgacgc cgccgcgacg ccggtggcgg gcggcgtgca gcgtccgcag ggcgcgccgg gcaacgcgcg cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.00107.d.A2.PW35409
PURIFICATION DATe: 4/11/2012
CONCENTRATION: 10.07mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 39
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVNAS APEKSAAPLP EVDVATVLSK TITDWQSYSG RLEAVEKVDV RPLVSGTIVS VNFKDGALVK KGDVLFVIDP RPYQAEVDRA AAQLAAAQAR NGYAQSDWQR AQRLIGDNAI AKRDYDEKQN AAREASANLK AAQAALETAR INLGYTRITA PVSGRVSRAE ITLGNVVSAG ASAAPLTTLV SVSPIYASFD ADEQTYLRYI GGAKDGRKVP VELGLANESG YSRQGVIDSV DNRLDTSSGT IRVRARFDNA DGSLVPGLYA RVKVGGSAPH PALLIDDAAI NTDQDKKFVF VVDQQGRVSY REVQLGAQHG NQRVIAGGLA AGDRIVVNGT QRVRPGEQVK PHLVPMTGGD DAAATPVAGG VQRPQGAPGN AR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggcg cgatccgcgt gaacgcgagc gcgccggaga aatcggcggc gccgctgccg gaggtcgacg tcgcgaccgt gctgtcgaag acgatcaccg actggcagtc ctactcgggc cgcctcgagg cggtcgagaa ggtcgacgtg cgtccgctcg tgtccggcac gatcgtgtcg gtgaacttca aggacggcgc gctcgtgaag aagggtgacg tgctgttcgt gatcgatccg cgtccgtatc aggccgaggt cgatcgcgcg gccgcgcagc tcgccgccgc gcaggcccgc aacggctacg cgcagagcga ctggcagcgc gcgcaacgcc tgatcggcga caacgcgatc gccaagcgcg actacgacga gaagcagaac gccgcgcgcg aggcatcggc gaacctgaag gcggcgcagg ccgcgctcga gaccgcacgc atcaacctcg gctacacgcg gatcaccgcg cccgtgtcgg gccgcgtgtc gcgcgcggag atcacgctcg gcaacgtcgt gtcggcgggc gcgtcggccg cgccgctcac gacgctcgta tcggtgtcgc cgatctacgc gtcgttcgac gcggacgagc agacgtatct gcgatacatc ggcggcgcga aggatggccg caaggtgccc gtcgagctcg gcctcgcgaa cgagagcggc tactcgcggc agggcgtgat cgattcggtc gacaaccggc tcgacacgtc gtccggcacg atccgcgtgc gcgcgcgctt cgacaacgcg gacggctcgc tcgtgcccgg cctctacgcg cgcgtgaagg tgggcggcag cgcgccgcat ccggcgcttc tcatcgacga cgcggcgatc aacaccgatc aggacaagaa gttcgtgttc gtcgtcgacc agcagggccg cgtgtcgtat cgcgaagtgc agctcggcgc gcagcacggc aatcagcgcg tgatcgcggg cggcctcgcg gcgggcgacc ggatcgtcgt gaacggcacg cagcgcgtgc gcccgggcga gcaggtgaag ccgcatctcg tgccgatgac gggcggcgac gacgccgccg cgacgccggt ggcgggcggc gtgcagcgtc cgcagggcgc gccgggcaac gcgcgcgca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAIRVNAS APEKSAAPLP EVDVATVLSK TITDWQSYSG RLEAVEKVDV RPLVSGTIVS VNFKDGALVK KGDVLFVIDP RPYQAEVDRA AAQLAAAQAR NGYAQSDWQR AQRLIGDNAI AKRDYDEKQN AAREASANLK AAQAALETAR INLGYTRITA PVSGRVSRAE ITLGNVVSAG ASAAPLTTLV SVSPIYASFD ADEQTYLRYI GGAKDGRKVP VELGLANESG YSRQGVIDSV DNRLDTSSGT IRVRARFDNA DGSLVPGLYA RVKVGGSAPH PALLIDDAAI NTDQDKKFVF VVDQQGRVSY REVQLGAQHG NQRVIAGGLA AGDRIVVNGT QRVRPGEQVK PHLVPMTGGD DAAATPVAGG VQRPQGAPGN ARA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGATC CGCGTGAACG CGAGCGCGCC GGAGAAATCG GCGGCGCCGC TGCCGGAGGT CGACGTCGCG ACCGTGCTGT CGAAGACGAT CACCGACTGG CAGTCCTACT CGGGCCGCCT CGAGGCGGTC GAGAAGGTCG ACGTGCGTCC GCTCGTGTCC GGCACGATCG TGTCGGTGAA CTTCAAGGAC GGCGCGCTCG TGAAGAAGGG TGACGTGCTG TTCGTGATCG ATCCGCGTCC GTATCAGGCC GAGGTCGATC GCGCGGCCGC GCAGCTCGCC GCCGCGCAGG CCCGCAACGG CTACGCGCAG AGCGACTGGC AGCGCGCGCA ACGCCTGATC GGCGACAACG CGATCGCCAA GCGCGACTAC GACGAGAAGC AGAACGCCGC GCGCGAGGCA TCGGCGAACC TGAAGGCGGC GCAGGCCGCG CTCGAGACCG CACGCATCAA CCTCGGCTAC ACGCGGATCA CCGCGCCCGT GTCGGGCCGC GTGTCGCGCG CGGAGATCAC GCTCGGCAAC GTCGTGTCGG CGGGCGCGTC GGCCGCGCCG CTCACGACGC TCGTATCGGT GTCGCCGATC TACGCGTCGT TCGACGCGGA CGAGCAGACG TATCTGCGAT ACATCGGCGG CGCGAAGGAT GGCCGCAAGG TGCCCGTCGA GCTCGGCCTC GCGAACGAGA GCGGCTACTC GCGGCAGGGC GTGATCGATT CGGTCGACAA CCGGCTCGAC ACGTCGTCCG GCACGATCCG CGTGCGCGCG CGCTTCGACA ACGCGGACGG CTCGCTCGTG CCCGGCCTCT ACGCGCGCGT GAAGGTGGGC GGCAGCGCGC CGCATCCGGC GCTTCTCATC GACGACGCGG CGATCAACAC CGATCAGGAC AAGAAGTTCG TGTTCGTCGT CGACCAGCAG GGCCGCGTGT CGTATCGCGA AGTGCAGCTC GGCGCGCAGC ACGGCAATCA GCGCGTGATC GCGGGCGGCC TCGCGGCGGG CGACCGGATC GTCGTGAACG GCACGCAGCG CGTGCGCCCG GGCGAGCAGG TGAAGCCGCA TCTCGTGCCG ATGACGGGCG GCGACGACGC CGCCGCGACG CCGGTGGCGG GCGGCGTGCA GCGTCCGCAG GGCGCGCCGG GCAACGCGCG CGCATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA