SSGCID: Target Page

ButhA.00107.a

RND multidrug efflux membrane fusion protein PA2019

CENTER ID:	ButhA.00107.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00107.a.A2.GE32412	Mature protein( ButhA.00107.a )		28	399

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00107.a.A2.PS01794	Mature protein( ButhA.00107.a )		28	399

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5290
RefSeq:	YP_442966.1
UniProt:	Q2SVT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKYEWARMRR RWAALAVAAF VAAGCGKRES AHEAAASREA SVVTVKKTSV PLSVELPGRL DAYRQAEVRA RVAGIVTART YEEGQEVKRG AVLFRIDPAP FKAARDAAAG ALEKAQAAHL AALDKRRRYD ELVRDRAVSE RDHTEALADE RQAKAAVASA RAELARAQLQ LDYATVTSPI DGRARRALVT EGALVGQDQA TPLTTVEQLD PIYVNFSQPA ADVESLRRAV KSGRAAGIAQ QDVEVTLVRP DGSTYARKGK LLFADLAVDP STDTVAMRAL FPNPERELLP GAYVRIALDR AVARDAILVP RDALLRTADS ATVKVVGENG KIRDVTVEAT QMKGRDWIVT RGLAGGERVV VVDAAQFDAG ATVKAVERGA AAQPASGAAA AAAPDKRQT

NT Sequence

ATGAAATACG AATGGGCACG CATGCGCCGC AGGTGGGCGG CGCTCGCGGT CGCGGCGTTC GTCGCGGCCG GCTGCGGCAA GCGCGAAAGC GCCCACGAGG CGGCCGCGTC GCGCGAGGCG AGCGTCGTCA CGGTGAAGAA GACGTCGGTG CCGCTGTCGG TCGAGCTGCC GGGCCGGCTC GACGCATACC GGCAGGCCGA GGTGCGCGCG CGAGTGGCGG GCATCGTGAC CGCGCGGACC TACGAGGAAG GGCAGGAGGT CAAGCGCGGC GCGGTGCTGT TCAGGATCGA TCCCGCGCCG TTCAAGGCGG CGCGCGACGC GGCCGCGGGC GCGCTCGAGA AGGCGCAGGC CGCGCATCTC GCGGCGCTCG ACAAGCGCCG CCGCTACGAC GAGCTCGTGC GCGACCGCGC GGTCAGCGAG CGCGACCACA CCGAGGCGCT CGCCGACGAG CGGCAGGCGA AGGCGGCCGT CGCGTCGGCG CGCGCCGAGC TCGCGCGCGC GCAACTGCAG CTCGACTACG CGACCGTCAC GTCGCCGATC GACGGCCGCG CGCGCCGCGC GCTCGTGACG GAGGGCGCGC TCGTCGGCCA GGATCAGGCG ACGCCGCTCA CGACCGTCGA GCAGCTCGAT CCGATCTACG TGAACTTCTC GCAGCCGGCC GCCGACGTCG AATCGCTGCG GCGCGCGGTG AAGAGCGGGC GCGCGGCGGG CATCGCGCAG CAGGACGTCG AGGTGACGCT CGTGCGTCCG GACGGCAGCA CTTACGCGCG CAAGGGCAAG CTGCTGTTCG CGGATCTCGC GGTCGATCCG TCCACCGACA CGGTCGCGAT GCGCGCGCTC TTTCCGAATC CGGAGCGCGA ACTGCTGCCG GGCGCGTACG TGCGGATCGC GCTCGATCGC GCGGTCGCGC GCGACGCGAT CCTCGTGCCG CGCGACGCGC TCCTGCGCAC GGCCGACAGC GCGACCGTCA AGGTCGTCGG CGAGAACGGA AAGATACGCG ACGTGACGGT CGAGGCCACG CAGATGAAAG GCCGCGACTG GATCGTCACG CGCGGGCTCG CGGGCGGCGA GCGCGTCGTC GTCGTCGACG CCGCGCAATT CGATGCGGGC GCGACCGTGA AGGCGGTCGA GCGCGGCGCC GCCGCGCAGC CGGCCTCCGG CGCCGCCGCG GCTGCCGCGC CCGACAAGCG CCAAACC

Details for ButhA.00107.a.A2.GE32412

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MRESAHEAAA SREASVVTVK KTSVPLSVEL PGRLDAYRQA EVRARVAGIV TARTYEEGQE VKRGAVLFRI DPAPFKAARD AAAGALEKAQ AAHLAALDKR RRYDELVRDR AVSERDHTEA LADERQAKAA VASARAELAR AQLQLDYATV TSPIDGRARR ALVTEGALVG QDQATPLTTV EQLDPIYVNF SQPAADVESL RRAVKSGRAA GIAQQDVEVT LVRPDGSTYA RKGKLLFADL AVDPSTDTVA MRALFPNPER ELLPGAYVRI ALDRAVARDA ILVPRDALLR TADSATVKVV GENGKIRDVT VEATQMKGRD WIVTRGLAGG ERVVVVDAAQ FDAGATVKAV ERGAAAQPAS GAAAAAAPDK

Validated NT Sequence

cgcttgtcgg gcgcggcagc cgcggcggcg ccggaggccg gctgcgcggc ggcgccgcgc tcgaccgcct tcacggtcgc gcccgcatcg aattgcgcgg cgtcgacgac gacgacgcgc tcgccgcccg cgagcccgcg cgtgacgatc cagtcgcggc ctttcatctg cgtggcctcg accgtcacgt cgcgtatctt tccgttctcg ccgacgacct tgacggtcgc gctgtcggcc gtgcgcagga gcgcgtcgcg cggcacgagg atcgcgtcgc gcgcgaccgc gcgatcgagc gcgatccgca cgtacgcgcc cggcagcagt tcgcgctccg gattcggaaa gagcgcgcgc atcgcgaccg tgtcggtgga cggatcgacc gcgagatccg cgaacagcag cttgcccttg cgcgcgtaag tgctgccgtc cggacgcacg agcgtcacct cgacgtcctg ctgcgcgatg cccgccgcgc gcccgctctt caccgcgcgc cgcagcgatt cgacgtcggc ggccggctgc gagaagttca cgtagatcgg atcgagctgc tcgacggtcg tgagcggcgt cgcctgatcc tggccgacga gcgcgccctc cgtcacgagc gcgcggcgcg cgcggccgtc gatcggcgac gtgacggtcg cgtagtcgag ctgcagttgc gcgcgcgcga gctcggcgcg cgccgacgcg acggccgcct tcgcctgccg ctcgtcggcg agcgcctcgg tgtggtcgcg ctcgctgacc gcgcggtcgc gcacgagctc gtcgtagcgg cggcgcttgt cgagcgccgc gagatgcgcg gcctgcgcct tctcgagcgc gcccgcggcc gcgtcgcgcg ccgccttgaa cggcgcggga tcgatcctga acagcaccgc gccgcgcttg acctcctgcc cttcctcgta ggtccgcgcg gtcacgatgc ccgccactcg cgcgcgcacc tcggcctgcc ggtatgcgtc gagccggccc ggcagctcga ccgacagcgg caccgacgtc ttcttcaccg tgacgacgct cgcctcgcgc gacgcggccg cctcgtgggc gctttcgcgc atcgaaccag gaccctgggt ctgagcttcc agggtaccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRESAHEAA ASREASVVTV KKTSVPLSVE LPGRLDAYRQ AEVRARVAGI VTARTYEEGQ EVKRGAVLFR IDPAPFKAAR DAAAGALEKA QAAHLAALDK RRRYDELVRD RAVSERDHTE ALADERQAKA AVASARAELA RAQLQLDYAT VTSPIDGRAR RALVTEGALV GQDQATPLTT VEQLDPIYVN FSQPAADVES LRRAVKSGRA AGIAQQDVEV TLVRPDGSTY ARKGKLLFAD LAVDPSTDTV AMRALFPNPE RELLPGAYVR IALDRAVARD AILVPRDALL RTADSATVKV VGENGKIRDV TVEATQMKGR DWIVTRGLAG GERVVVVDAA QFDAGATVKA VERGAAAQPA SGAAAAAAPD KRQT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgaaagc gcccacgagg cggccgcgtc gcgcgaggcg agcgtcgtca cggtgaagaa gacgtcggtg ccgctgtcgg tcgagctgcc gggccggctc gacgcatacc ggcaggccga ggtgcgcgcg cgagtggcgg gcatcgtgac cgcgcggacc tacgaggaag ggcaggaggt caagcgcggc gcggtgctgt tcaggatcga tcccgcgccg ttcaaggcgg cgcgcgacgc ggccgcgggc gcgctcgaga aggcgcaggc cgcgcatctc gcggcgctcg acaagcgccg ccgctacgac gagctcgtgc gcgaccgcgc ggtcagcgag cgcgaccaca ccgaggcgct cgccgacgag cggcaggcga aggcggccgt cgcgtcggcg cgcgccgagc tcgcgcgcgc gcaactgcag ctcgactacg cgaccgtcac gtcgccgatc gacggccgcg cgcgccgcgc gctcgtgacg gagggcgcgc tcgtcggcca ggatcaggcg acgccgctca cgaccgtcga gcagctcgat ccgatctacg tgaacttctc gcagccggcc gccgacgtcg aatcgctgcg gcgcgcggtg aagagcgggc gcgcggcggg catcgcgcag caggacgtcg aggtgacgct cgtgcgtccg gacggcagca cttacgcgcg caagggcaag ctgctgttcg cggatctcgc ggtcgatccg tccaccgaca cggtcgcgat gcgcgcgctc tttccgaatc cggagcgcga actgctgccg ggcgcgtacg tgcggatcgc gctcgatcgc gcggtcgcgc gcgacgcgat cctcgtgccg cgcgacgcgc tcctgcgcac ggccgacagc gcgaccgtca aggtcgtcgg cgagaacgga aagatacgcg acgtgacggt cgaggccacg cagatgaaag gccgcgactg gatcgtcacg cgcgggctcg cgggcggcga gcgcgtcgtc gtcgtcgacg ccgcgcaatt cgatgcgggc gcgaccgtga aggcggtcga gcgcggcgcc gccgcgcagc cggcctccgg cgccgccgcg gctgccgcgc ccgacaagcg ccaaacctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for ButhA.00107.a.A2.PS01794

PURIFICATION DATe:	3/26/2013
CONCENTRATION:	56.3mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCGAAAGC GCCCACGAGG CGGCCGCGTC GCGCGAGGCG AGCGTCGTCA CGGTGAAGAA GACGTCGGTG CCGCTGTCGG TCGAGCTGCC GGGCCGGCTC GACGCATACC GGCAGGCCGA GGTGCGCGCG CGAGTGGCGG GCATCGTGAC CGCGCGGACC TACGAGGAAG GGCAGGAGGT CAAGCGCGGC GCGGTGCTGT TCAGGATCGA TCCCGCGCCG TTCAAGGCGG CGCGCGACGC GGCCGCGGGC GCGCTCGAGA AGGCGCAGGC CGCGCATCTC GCGGCGCTCG ACAAGCGCCG CCGCTACGAC GAGCTCGTGC GCGACCGCGC GGTCAGCGAG CGCGACCACA CCGAGGCGCT CGCCGACGAG CGGCAGGCGA AGGCGGCCGT CGCGTCGGCG CGCGCCGAGC TCGCGCGCGC GCAACTGCAG CTCGACTACG CGACCGTCAC GTCGCCGATC GACGGCCGCG CGCGCCGCGC GCTCGTGACG GAGGGCGCGC TCGTCGGCCA GGATCAGGCG ACGCCGCTCA CGACCGTCGA GCAGCTCGAT CCGATCTACG TGAACTTCTC GCAGCCGGCC GCCGACGTCG AATCGCTGCG GCGCGCGGTG AAGAGCGGGC GCGCGGCGGG CATCGCGCAG CAGGACGTCG AGGTGACGCT CGTGCGTCCG GACGGCAGCA CTTACGCGCG CAAGGGCAAG CTGCTGTTCG CGGATCTCGC GGTCGATCCG TCCACCGACA CGGTCGCGAT GCGCGCGCTC TTTCCGAATC CGGAGCGCGA ACTGCTGCCG GGCGCGTACG TGCGGATCGC GCTCGATCGC GCGGTCGCGC GCGACGCGAT CCTCGTGCCG CGCGACGCGC TCCTGCGCAC GGCCGACAGC GCGACCGTCA AGGTCGTCGG CGAGAACGGA AAGATACGCG ACGTGACGGT CGAGGCCACG CAGATGAAAG GCCGCGACTG GATCGTCACG CGCGGGCTCG CGGGCGGCGA GCGCGTCGTC GTCGTCGACG CCGCGCAATT CGATGCGGGC GCGACCGTGA AGGCGGTCGA GCGCGGCGCC GCCGCGCAGC CGGCCTCCGG CGCCGCCGCG GCTGCCGCGC CCGACAAGCG CCAAACCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA