ButhA.00103.a

Uracil-DNA glycosylase (UDG) (EC 3.2.2.27) (BTH_I2914) (ung)

CENTER ID: ButhA.00103.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00103.a.A1.GE32880 full length 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00103.a.A1.PS01230 full length 1 271

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5794
RefSeq: YP_443421.1
UniProt: Q2SUH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKPSSSRPM QQPSLFDDPA PPAPPAPKGD AFTPTLESQF DALPPDWRAI LEPFVASDAY APLCRFVDGE RAAGKAIYPA DVFRALRLTH PDDVKVVILG QDPYHGEDRG IPQAHGLAFS VPPGVRPPPS LRNIFKEISA DFGYEAPRHG CLDTWASQGV LLLNTVLTVE RSSAASHAKR GWEKCTDTLI HELATRHRHL VFMLWGAHAQ AKRALFDPRE HCVLEAPHPS PLSAHRGFLG CRHFALANDY LVKHGRAPID WRLPDAAETL A
NT Sequence
atgaccaagc cctcttcgtc ccgcccgatg caacaaccct cgctgttcga cgatcctgct ccgcccgcgc cgccggcccc gaagggcgac gcattcacgc ccaccctcga atcgcaattc gacgcgttgc cgcccgactg gcgcgcgatt ctcgagccgt tcgtcgcgag cgacgcctat gcgccgctct gccgcttcgt cgacggcgag cgtgcggccg gcaaggcgat ttatccggcc gacgtgttcc gcgcgctgcg gctcacgcat ccggacgacg tgaaggtcgt gattctcggc caggacccgt atcacggcga ggaccgcggc atcccgcagg cgcacgggct cgcgttctcg gtgccgcccg gcgtgcggcc gccgccgtcg ctgcgcaaca tcttcaagga aatctcggcc gacttcggct atgaagcgcc ccgccacggc tgtctcgaca cctgggcgag ccagggcgtg ctgctgctca acacggtgct gaccgtcgag cgctcgtcgg ccgcgagcca cgcgaagcgc ggctgggaaa aatgcaccga cacgctgatc cacgaactcg cgacacgcca ccgccacctc gtgttcatgc tgtggggcgc gcacgcgcag gcaaagcgcg cgctattcga tccgcgcgag cactgcgtgc tcgaagcgcc gcatccgtcg ccgctgtccg cgcaccgcgg cttcctcggc tgccgtcatt tcgcgctcgc gaacgactat ctcgtcaaac acgggcgcgc gccgatcgac tggcggctgc cggacgcagc cgagacgctc gcctga
Details for ButhA.00103.a.A1.GE32880
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKPSSSRP MQQPSLFDDP APPAPPAPKG DAFTPTLESQ FDALPPDWRA ILEPFVASDA YAPLCRFVDG ERAAGKAIYP ADVFRALRLT HPDDVKVVIL GQDPYHGEDR GIPQAHGLAF SVPPGVRPPP SLRNIFKEIS ADFGYEAPRH GCLDTWASQG VLLLNTVLTV ERSSAASHAK RGWEKCTDTL IHELATRHRH LVFMLWGAHA QAKRALFDPR EHCVLEAPHP SPLSAHRGFL GCRHFALAND YLVKHGRAPI DWRLPDAAET LA
Validated NT Sequence
ttattaggcg agcgtctcgg ctgcgtccgg cagccgccag tcgatcggcg cgcgcccgtg tttgacgaga tagtcgttcg cgagcgcgaa atgacggcag ccgaggaagc cgcggtgcgc ggacagcggc gacggatgcg gcgcttcgag cacgcagtgc tcgcgcggat cgaatagcgc gcgctttgcc tgcgcgtgcg cgccccacag catgaacacg aggtggcggt ggcgtgtcgc gagttcgtgg atcagcgtgt cggtgcattt ttcccagccg cgcttcgcgt ggctcgcggc cgacgagcgc tcgacggtca gcaccgtgtt gagcagcagc acgccctggc tcgcccaggt gtcgagacag ccgtggcggg gcgcttcata gccgaagtcg gccgagattt ccttgaagat gttgcgcagc gacggcggcg gccgcacgcc gggcggcacc gagaacgcga gcccgtgcgc ctgcgggatg ccgcggtcct cgccgtgata cgggtcctgg ccgagaatca cgaccttcac gtcgtccgga tgcgtgagcc gcagcgcgcg gaacacgtcg gccggataaa tcgccttgcc ggccgcacgc tcgccgtcga cgaagcggca gagcggcgca taggcgtcgc tcgcgacgaa cggctcgaga atcgcgcgcc agtcgggcgg caacgcgtcg aattgcgatt cgagggtggg cgtgaatgcg tcgcccttcg gggccggcgg cgcgggcgga gcaggatcgt cgaacagcga gggttgttgc atcgggcggg acgaagaggg cttggtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKPSSSRP MQQPSLFDDP APPAPPAPKG DAFTPTLESQ FDALPPDWRA ILEPFVASDA YAPLCRFVDG ERAAGKAIYP ADVFRALRLT HPDDVKVVIL GQDPYHGEDR GIPQAHGLAF SVPPGVRPPP SLRNIFKEIS ADFGYEAPRH GCLDTWASQG VLLLNTVLTV ERSSAASHAK RGWEKCTDTL IHELATRHRH LVFMLWGAHA QAKRALFDPR EHCVLEAPHP SPLSAHRGFL GCRHFALAND YLVKHGRAPI DWRLPDAAET LA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaagccc tcttcgtccc gcccgatgca acaaccctcg ctgttcgacg atcctgctcc gcccgcgccg ccggccccga agggcgacgc attcacgccc accctcgaat cgcaattcga cgcgttgccg cccgactggc gcgcgattct cgagccgttc gtcgcgagcg acgcctatgc gccgctctgc cgcttcgtcg acggcgagcg tgcggccggc aaggcgattt atccggccga cgtgttccgc gcgctgcggc tcacgcatcc ggacgacgtg aaggtcgtga ttctcggcca ggacccgtat cacggcgagg accgcggcat cccgcaggcg cacgggctcg cgttctcggt gccgcccggc gtgcggccgc cgccgtcgct gcgcaacatc ttcaaggaaa tctcggccga cttcggctat gaagcgcccc gccacggctg tctcgacacc tgggcgagcc agggcgtgct gctgctcaac acggtgctga ccgtcgagcg ctcgtcggcc gcgagccacg cgaagcgcgg ctgggaaaaa tgcaccgaca cgctgatcca cgaactcgcg acacgccacc gccacctcgt gttcatgctg tggggcgcgc acgcgcaggc aaagcgcgcg ctattcgatc cgcgcgagca ctgcgtgctc gaagcgccgc atccgtcgcc gctgtccgcg caccgcggct tcctcggctg ccgtcatttc gcgctcgcga acgactatct cgtcaaacac gggcgcgcgc cgatcgactg gcggctgccg gacgcagccg agacgctcgc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.00103.a.A1.PS01230
PURIFICATION DATe: 10/6/2011
CONCENTRATION: 26.44mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKPSSSRP MQQPSLFDDP APPAPPAPKG DAFTPTLESQ FDALPPDWRA ILEPFVASDA YAPLCRFVDG ERAAGKAIYP ADVFRALRLT HPDDVKVVIL GQDPYHGEDR GIPQAHGLAF SVPPGVRPPP SLRNIFKEIS ADFGYEAPRH GCLDTWASQG VLLLNTVLTV ERSSAASHAK RGWEKCTDTL IHELATRHRH LVFMLWGAHA QAKRALFDPR EHCVLEAPHP SPLSAHRGFL GCRHFALAND YLVKHGRAPI DWRLPDAAET LA
Validated NT Sequence
ttattaggcg agcgtctcgg ctgcgtccgg cagccgccag tcgatcggcg cgcgcccgtg tttgacgaga tagtcgttcg cgagcgcgaa atgacggcag ccgaggaagc cgcggtgcgc ggacagcggc gacggatgcg gcgcttcgag cacgcagtgc tcgcgcggat cgaatagcgc gcgctttgcc tgcgcgtgcg cgccccacag catgaacacg aggtggcggt ggcgtgtcgc gagttcgtgg atcagcgtgt cggtgcattt ttcccagccg cgcttcgcgt ggctcgcggc cgacgagcgc tcgacggtca gcaccgtgtt gagcagcagc acgccctggc tcgcccaggt gtcgagacag ccgtggcggg gcgcttcata gccgaagtcg gccgagattt ccttgaagat gttgcgcagc gacggcggcg gccgcacgcc gggcggcacc gagaacgcga gcccgtgcgc ctgcgggatg ccgcggtcct cgccgtgata cgggtcctgg ccgagaatca cgaccttcac gtcgtccgga tgcgtgagcc gcagcgcgcg gaacacgtcg gccggataaa tcgccttgcc ggccgcacgc tcgccgtcga cgaagcggca gagcggcgca taggcgtcgc tcgcgacgaa cggctcgaga atcgcgcgcc agtcgggcgg caacgcgtcg aattgcgatt cgagggtggg cgtgaatgcg tcgcccttcg gggccggcgg cgcgggcgga gcaggatcgt cgaacagcga gggttgttgc atcgggcggg acgaagaggg cttggtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKPSSSRP MQQPSLFDDP APPAPPAPKG DAFTPTLESQ FDALPPDWRA ILEPFVASDA YAPLCRFVDG ERAAGKAIYP ADVFRALRLT HPDDVKVVIL GQDPYHGEDR GIPQAHGLAF SVPPGVRPPP SLRNIFKEIS ADFGYEAPRH GCLDTWASQG VLLLNTVLTV ERSSAASHAK RGWEKCTDTL IHELATRHRH LVFMLWGAHA QAKRALFDPR EHCVLEAPHP SPLSAHRGFL GCRHFALAND YLVKHGRAPI DWRLPDAAET LA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAAGCCC TCTTCGTCCC GCCCGATGCA ACAACCCTCG CTGTTCGACG ATCCTGCTCC GCCCGCGCCG CCGGCCCCGA AGGGCGACGC ATTCACGCCC ACCCTCGAAT CGCAATTCGA CGCGTTGCCG CCCGACTGGC GCGCGATTCT CGAGCCGTTC GTCGCGAGCG ACGCCTATGC GCCGCTCTGC CGCTTCGTCG ACGGCGAGCG TGCGGCCGGC AAGGCGATTT ATCCGGCCGA CGTGTTCCGC GCGCTGCGGC TCACGCATCC GGACGACGTG AAGGTCGTGA TTCTCGGCCA GGACCCGTAT CACGGCGAGG ACCGCGGCAT CCCGCAGGCG CACGGGCTCG CGTTCTCGGT GCCGCCCGGC GTGCGGCCGC CGCCGTCGCT GCGCAACATC TTCAAGGAAA TCTCGGCCGA CTTCGGCTAT GAAGCGCCCC GCCACGGCTG TCTCGACACC TGGGCGAGCC AGGGCGTGCT GCTGCTCAAC ACGGTGCTGA CCGTCGAGCG CTCGTCGGCC GCGAGCCACG CGAAGCGCGG CTGGGAAAAA TGCACCGACA CGCTGATCCA CGAACTCGCG ACACGCCACC GCCACCTCGT GTTCATGCTG TGGGGCGCGC ACGCGCAGGC AAAGCGCGCG CTATTCGATC CGCGCGAGCA CTGCGTGCTC GAAGCGCCGC ATCCGTCGCC GCTGTCCGCG CACCGCGGCT TCCTCGGCTG CCGTCATTTC GCGCTCGCGA ACGACTATCT CGTCAAACAC GGGCGCGCGC CGATCGACTG GCGGCTGCCG GACGCAGCCG AGACGCTCGC CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA