ButhA.00101.a
Ferredoxin--NADP reductase
| CENTER ID: | ButhA.00101.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00101.a.A1.GE32410 | Full length( ButhA.00101.a ) | 1 | 271 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00101.a.A1.PS01323 | Full length( ButhA.00101.a ) | 1 | 271 | ||
| ButhA.00101.a.A1.PS01324 | Full length( ButhA.00101.a ) | 1 | 271 |
Structures
4F7D
DEPOSITED: 5/15/2012
DETERMINATION: XRay
CLONE: ButhA.00101.a.A1.GE32410
PROTEIN: ButhA.00101.a.A1.PS01323
4FK8
DEPOSITED: 6/12/2012
DETERMINATION: XRay
CLONE: ButhA.00101.a.A1.GE32410
PROTEIN: ButhA.00101.a.A1.PS01323
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_137342 |
| PATRIC ID: | fig|271848.6.peg.2861 |
| RefSeq: | YP_440769.1 |
| UniProt: | Q2T230 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGPLWGPLYA PVSESMSKFD TATVLSVHHW TDTLFSFTCT RDQALRFNNG EFTMVGLEVD GKPLTRAYSI VSPNYEEHLE FFSIKVQNGP LTSRLQHLKV GDPVLIGKKP TGTLVADNLL PGKTLWMLST GTGLAPFMSI IRDPDIYERF DKVVLTHTCR LKGELAYMDY IKHDLPGHEY LGDVIREKLV YYPTVTREEF ENEGRITDLI ASGKLFTDLD MPPFSPEQDR VMLCGSTAML KDTTELLKKA GLVEGKNSAP GHYVIERAFV D
NT Sequence
TTGGGCCCGC TTTGGGGCCC GTTATACGCG CCTGTTTCCG AATCCATGAG CAAATTCGAC ACTGCTACCG TCCTGTCCGT CCACCACTGG ACCGACACGC TGTTCAGCTT CACCTGCACC CGCGACCAAG CCCTGCGCTT CAACAACGGC GAGTTCACGA TGGTCGGCCT GGAAGTCGAC GGCAAGCCGC TCACGCGCGC CTACAGCATC GTGAGCCCGA ACTACGAAGA ACATCTCGAA TTCTTCAGCA TCAAGGTGCA GAACGGGCCG CTCACGTCGC GGCTGCAGCA CCTGAAGGTC GGCGATCCGG TTCTGATCGG CAAGAAGCCG ACGGGCACGC TCGTCGCCGA CAACCTGCTG CCCGGCAAGA CGCTGTGGAT GCTGTCGACG GGCACGGGCC TCGCGCCGTT CATGTCGATC ATCCGCGATC CGGATATCTA CGAACGCTTC GACAAGGTGG TCCTCACGCA CACGTGCCGC CTGAAGGGCG AACTCGCGTA CATGGACTAC ATCAAGCACG ACCTGCCGGG CCACGAGTAC CTCGGCGACG TGATCCGCGA GAAGCTCGTC TACTACCCGA CCGTCACGCG CGAGGAGTTC GAGAACGAAG GCCGGATCAC CGATCTGATC GCATCCGGCA AGCTGTTCAC GGATCTGGAC ATGCCGCCGT TCTCGCCCGA GCAAGATCGC GTGATGCTGT GCGGCAGCAC CGCGATGCTG AAGGACACGA CCGAGTTGCT GAAGAAGGCG GGCCTCGTCG AAGGCAAGAA CAGCGCCCCC GGTCACTACG TGATCGAGCG CGCGTTCGTC GAC
Details for ButhA.00101.a.A1.GE32410
| HARVESTED ON: | 11/8/2011 |
| SEQUENCED ON: | 11/11/2011 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MLGPLWGPLY APVSESMSKF DTATVLSVHH WTDTLFSFTC TRDQALRFNN GEFTMVGLEV DGKPLTRAYS IVSPNYEEHL EFFSIKVQNG PLTSRLQHLK VGDPVLIGKK PTGTLVADNL LPGKTLWMLS TGTGLAPFMS IIRDPDIYER FDKVVLTHTC RLKGELAYMD YIKHDLPGHE YLGDVIREKL VYYPTVTREE FENEGRITDL IASGKLFTDL DMPPFSPEQD RVMLCGSTAM LKDTTELLKK AGLVEGKNSA PGHYVIERAF VD
Validated NT Sequence
ttattagtcg acgaacgcgc gctcgatcac gtagtgaccg ggggcgctgt tcttgccttc gacgaggccc gccttcttca gcaactcggt cgtgtccttc agcatcgcgg tgctgccgca cagcatcacg cgatcttgct cgggcgagaa cggcggcatg tccagatccg tgaacagctt gccggatgcg atcagatcgg tgatccggcc ttcgttctcg aactcctcgc gcgtgacggt cgggtagtag acgagcttct cgcggatcac gtcgccgagg tactcgtggc ccggcaggtc gtgcttgatg tagtccatgt acgcgagttc gcccttcagg cggcacgtgt gcgtgaggac caccttgtcg aagcgttcgt agatatccgg atcgcggatg atcgacatga acggcgcgag gcccgtgccc gtcgacagca tccacagcgt cttgccgggc agcaggttgt cggcgacgag cgtgcccgtc ggcttcttgc cgatcagaac cggatcgccg accttcaggt gctgcagccg cgacgtgagc ggcccgttct gcaccttgat gctgaagaat tcgagatgtt cttcgtagtt cgggctcacg atgctgtagg cgcgcgtgag cggcttgccg tcgacttcca ggccgaccat cgtgaactcg ccgttgttga agcgcagggc ttggtcgcgg gtgcaggtga agctgaacag cgtgtcggtc cagtggtgga cggacaggac ggtagcagtg tcgaatttgc tcatggattc ggaaacaggc gcgtataacg ggccccaaag cgggcccaac atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGPLWGPL YAPVSESMSK FDTATVLSVH HWTDTLFSFT CTRDQALRFN NGEFTMVGLE VDGKPLTRAY SIVSPNYEEH LEFFSIKVQN GPLTSRLQHL KVGDPVLIGK KPTGTLVADN LLPGKTLWML STGTGLAPFM SIIRDPDIYE RFDKVVLTHT CRLKGELAYM DYIKHDLPGH EYLGDVIREK LVYYPTVTRE EFENEGRITD LIASGKLFTD LDMPPFSPEQ DRVMLCGSTA MLKDTTELLK KAGLVEGKNS APGHYVIERA FVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgggcccg ctttggggcc cgttatacgc gcctgtttcc gaatccatga gcaaattcga cactgctacc gtcctgtccg tccaccactg gaccgacacg ctgttcagct tcacctgcac ccgcgaccaa gccctgcgct tcaacaacgg cgagttcacg atggtcggcc tggaagtcga cggcaagccg ctcacgcgcg cctacagcat cgtgagcccg aactacgaag aacatctcga attcttcagc atcaaggtgc agaacgggcc gctcacgtcg cggctgcagc acctgaaggt cggcgatccg gttctgatcg gcaagaagcc gacgggcacg ctcgtcgccg acaacctgct gcccggcaag acgctgtgga tgctgtcgac gggcacgggc ctcgcgccgt tcatgtcgat catccgcgat ccggatatct acgaacgctt cgacaaggtg gtcctcacgc acacgtgccg cctgaagggc gaactcgcgt acatggacta catcaagcac gacctgccgg gccacgagta cctcggcgac gtgatccgcg agaagctcgt ctactacccg accgtcacgc gcgaggagtt cgagaacgaa ggccggatca ccgatctgat cgcatccggc aagctgttca cggatctgga catgccgccg ttctcgcccg agcaagatcg cgtgatgctg tgcggcagca ccgcgatgct gaaggacacg accgagttgc tgaagaaggc gggcctcgtc gaaggcaaga acagcgcccc cggtcactac gtgatcgagc gcgcgttcgt cgactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.00101.a.A1.PS01323
| PURIFICATION DATe: | 12/28/2011 |
| CONCENTRATION: | 86.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MLGPLWGPLY APVSESMSKF DTATVLSVHH WTDTLFSFTC TRDQALRFNN GEFTMVGLEV DGKPLTRAYS IVSPNYEEHL EFFSIKVQNG PLTSRLQHLK VGDPVLIGKK PTGTLVADNL LPGKTLWMLS TGTGLAPFMS IIRDPDIYER FDKVVLTHTC RLKGELAYMD YIKHDLPGHE YLGDVIREKL VYYPTVTREE FENEGRITDL IASGKLFTDL DMPPFSPEQD RVMLCGSTAM LKDTTELLKK AGLVEGKNSA PGHYVIERAF VD
Validated NT Sequence
ttattagtcg acgaacgcgc gctcgatcac gtagtgaccg ggggcgctgt tcttgccttc gacgaggccc gccttcttca gcaactcggt cgtgtccttc agcatcgcgg tgctgccgca cagcatcacg cgatcttgct cgggcgagaa cggcggcatg tccagatccg tgaacagctt gccggatgcg atcagatcgg tgatccggcc ttcgttctcg aactcctcgc gcgtgacggt cgggtagtag acgagcttct cgcggatcac gtcgccgagg tactcgtggc ccggcaggtc gtgcttgatg tagtccatgt acgcgagttc gcccttcagg cggcacgtgt gcgtgaggac caccttgtcg aagcgttcgt agatatccgg atcgcggatg atcgacatga acggcgcgag gcccgtgccc gtcgacagca tccacagcgt cttgccgggc agcaggttgt cggcgacgag cgtgcccgtc ggcttcttgc cgatcagaac cggatcgccg accttcaggt gctgcagccg cgacgtgagc ggcccgttct gcaccttgat gctgaagaat tcgagatgtt cttcgtagtt cgggctcacg atgctgtagg cgcgcgtgag cggcttgccg tcgacttcca ggccgaccat cgtgaactcg ccgttgttga agcgcagggc ttggtcgcgg gtgcaggtga agctgaacag cgtgtcggtc cagtggtgga cggacaggac ggtagcagtg tcgaatttgc tcatggattc ggaaacaggc gcgtataacg ggccccaaag cgggcccaac atcgaaccag gaccctgggt ctgagcttcc aggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGPLWGPL YAPVSESMSK FDTATVLSVH HWTDTLFSFT CTRDQALRFN NGEFTMVGLE VDGKPLTRAY SIVSPNYEEH LEFFSIKVQN GPLTSRLQHL KVGDPVLIGK KPTGTLVADN LLPGKTLWML STGTGLAPFM SIIRDPDIYE RFDKVVLTHT CRLKGELAYM DYIKHDLPGH EYLGDVIREK LVYYPTVTRE EFENEGRITD LIASGKLFTD LDMPPFSPEQ DRVMLCGSTA MLKDTTELLK KAGLVEGKNS APGHYVIERA FVD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGGCCCG CTTTGGGGCC CGTTATACGC GCCTGTTTCC GAATCCATGA GCAAATTCGA CACTGCTACC GTCCTGTCCG TCCACCACTG GACCGACACG CTGTTCAGCT TCACCTGCAC CCGCGACCAA GCCCTGCGCT TCAACAACGG CGAGTTCACG ATGGTCGGCC TGGAAGTCGA CGGCAAGCCG CTCACGCGCG CCTACAGCAT CGTGAGCCCG AACTACGAAG AACATCTCGA ATTCTTCAGC ATCAAGGTGC AGAACGGGCC GCTCACGTCG CGGCTGCAGC ACCTGAAGGT CGGCGATCCG GTTCTGATCG GCAAGAAGCC GACGGGCACG CTCGTCGCCG ACAACCTGCT GCCCGGCAAG ACGCTGTGGA TGCTGTCGAC GGGCACGGGC CTCGCGCCGT TCATGTCGAT CATCCGCGAT CCGGATATCT ACGAACGCTT CGACAAGGTG GTCCTCACGC ACACGTGCCG CCTGAAGGGC GAACTCGCGT ACATGGACTA CATCAAGCAC GACCTGCCGG GCCACGAGTA CCTCGGCGAC GTGATCCGCG AGAAGCTCGT CTACTACCCG ACCGTCACGC GCGAGGAGTT CGAGAACGAA GGCCGGATCA CCGATCTGAT CGCATCCGGC AAGCTGTTCA CGGATCTGGA CATGCCGCCG TTCTCGCCCG AGCAAGATCG CGTGATGCTG TGCGGCAGCA CCGCGATGCT GAAGGACACG ACCGAGTTGC TGAAGAAGGC GGGCCTCGTC GAAGGCAAGA ACAGCGCCCC CGGTCACTAC GTGATCGAGC GCGCGTTCGT CGACTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for ButhA.00101.a.A1.PS01324
| PURIFICATION DATe: | 12/28/2011 |
| CONCENTRATION: | 42.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MLGPLWGPLY APVSESMSKF DTATVLSVHH WTDTLFSFTC TRDQALRFNN GEFTMVGLEV DGKPLTRAYS IVSPNYEEHL EFFSIKVQNG PLTSRLQHLK VGDPVLIGKK PTGTLVADNL LPGKTLWMLS TGTGLAPFMS IIRDPDIYER FDKVVLTHTC RLKGELAYMD YIKHDLPGHE YLGDVIREKL VYYPTVTREE FENEGRITDL IASGKLFTDL DMPPFSPEQD RVMLCGSTAM LKDTTELLKK AGLVEGKNSA PGHYVIERAF VD
Validated NT Sequence
ttattagtcg acgaacgcgc gctcgatcac gtagtgaccg ggggcgctgt tcttgccttc gacgaggccc gccttcttca gcaactcggt cgtgtccttc agcatcgcgg tgctgccgca cagcatcacg cgatcttgct cgggcgagaa cggcggcatg tccagatccg tgaacagctt gccggatgcg atcagatcgg tgatccggcc ttcgttctcg aactcctcgc gcgtgacggt cgggtagtag acgagcttct cgcggatcac gtcgccgagg tactcgtggc ccggcaggtc gtgcttgatg tagtccatgt acgcgagttc gcccttcagg cggcacgtgt gcgtgaggac caccttgtcg aagcgttcgt agatatccgg atcgcggatg atcgacatga acggcgcgag gcccgtgccc gtcgacagca tccacagcgt cttgccgggc agcaggttgt cggcgacgag cgtgcccgtc ggcttcttgc cgatcagaac cggatcgccg accttcaggt gctgcagccg cgacgtgagc ggcccgttct gcaccttgat gctgaagaat tcgagatgtt cttcgtagtt cgggctcacg atgctgtagg cgcgcgtgag cggcttgccg tcgacttcca ggccgaccat cgtgaactcg ccgttgttga agcgcagggc ttggtcgcgg gtgcaggtga agctgaacag cgtgtcggtc cagtggtgga cggacaggac ggtagcagtg tcgaatttgc tcatggattc ggaaacaggc gcgtataacg ggccccaaag cgggcccaac atcgaaccag gaccctgggt ctgagcttcc aggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLGPLWGPL YAPVSESMSK FDTATVLSVH HWTDTLFSFT CTRDQALRFN NGEFTMVGLE VDGKPLTRAY SIVSPNYEEH LEFFSIKVQN GPLTSRLQHL KVGDPVLIGK KPTGTLVADN LLPGKTLWML STGTGLAPFM SIIRDPDIYE RFDKVVLTHT CRLKGELAYM DYIKHDLPGH EYLGDVIREK LVYYPTVTRE EFENEGRITD LIASGKLFTD LDMPPFSPEQ DRVMLCGSTA MLKDTTELLK KAGLVEGKNS APGHYVIERA FVD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGGCCCG CTTTGGGGCC CGTTATACGC GCCTGTTTCC GAATCCATGA GCAAATTCGA CACTGCTACC GTCCTGTCCG TCCACCACTG GACCGACACG CTGTTCAGCT TCACCTGCAC CCGCGACCAA GCCCTGCGCT TCAACAACGG CGAGTTCACG ATGGTCGGCC TGGAAGTCGA CGGCAAGCCG CTCACGCGCG CCTACAGCAT CGTGAGCCCG AACTACGAAG AACATCTCGA ATTCTTCAGC ATCAAGGTGC AGAACGGGCC GCTCACGTCG CGGCTGCAGC ACCTGAAGGT CGGCGATCCG GTTCTGATCG GCAAGAAGCC GACGGGCACG CTCGTCGCCG ACAACCTGCT GCCCGGCAAG ACGCTGTGGA TGCTGTCGAC GGGCACGGGC CTCGCGCCGT TCATGTCGAT CATCCGCGAT CCGGATATCT ACGAACGCTT CGACAAGGTG GTCCTCACGC ACACGTGCCG CCTGAAGGGC GAACTCGCGT ACATGGACTA CATCAAGCAC GACCTGCCGG GCCACGAGTA CCTCGGCGAC GTGATCCGCG AGAAGCTCGT CTACTACCCG ACCGTCACGC GCGAGGAGTT CGAGAACGAA GGCCGGATCA CCGATCTGAT CGCATCCGGC AAGCTGTTCA CGGATCTGGA CATGCCGCCG TTCTCGCCCG AGCAAGATCG CGTGATGCTG TGCGGCAGCA CCGCGATGCT GAAGGACACG ACCGAGTTGC TGAAGAAGGC GGGCCTCGTC GAAGGCAAGA ACAGCGCCCC CGGTCACTAC GTGATCGAGC GCGCGTTCGT CGACTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA