ButhA.00100.b
L-arabinose ABC transporter, periplasmic L-arabinose-binding protein
| CENTER ID: | ButhA.00100.b |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00100.b.B2.GE36103 | Mature protein( ButhA.00100.b ) | 27 | 333 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00100.b.B2.PS01715 | Mature protein( ButhA.00100.b ) | 27 | 333 | ||
| ButhA.00100.b.B2.PS01716 | Mature protein( ButhA.00100.b ) | 27 | 333 |
Structures
4KZK
DEPOSITED: 5/30/2013
DETERMINATION: XRay
CLONE: ButhA.00100.b.B2.GE36103
PROTEIN: ButhA.00100.b.B2.PS01715
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_215383 |
| PATRIC ID: | fig|271848.6.peg.1326 |
| RefSeq: | YP_439392.1 |
| UniProt: | Q2T607 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLRWLQAAL VCTSLAAGLS AAAPARAQGA APVKIGFVVK QPDDPWFQDE WRFAEQAAKD KHFTLVKIAA PSGEKVSTAL DSLAAQKAQG VIICAPDVKL GPGIAAKAKR YGMKLMSVDD QLVDGRGAPL ADVPHMGISA YRIGRQVGDA IAAEAKRRGW NPAEVGVLRL AYDQLPTARE RTTGAVDALK AAGFAAANVV DAPEMTADTE GAFNAANIAF TKHRNFRHWV AFGSNDDTTV GAVRAGEGRG IGTDDMIAVG INGSQVALNE FAKPKPTGFF GSILLNPRLH GYDTSVNMYD WITQNRTPPP LVLTSGTLIT RANEKTARAQ LGL
NT Sequence
ATGGGATTGC GTTGGCTTCA AGCCGCCCTC GTCTGCACGA GTCTCGCCGC CGGTTTGTCG GCGGCGGCAC CCGCGCGTGC GCAAGGCGCG GCCCCGGTGA AGATCGGCTT TGTCGTCAAG CAGCCCGACG ACCCGTGGTT TCAGGACGAA TGGCGCTTCG CCGAGCAGGC GGCGAAGGAC AAGCACTTCA CGCTCGTGAA GATCGCCGCG CCGAGCGGCG AGAAAGTGTC GACCGCGCTC GACAGCCTTG CCGCGCAAAA GGCGCAAGGC GTGATCATCT GCGCGCCCGA CGTGAAGCTC GGTCCCGGCA TCGCCGCGAA GGCGAAGCGC TACGGAATGA AGCTGATGTC GGTCGACGAT CAACTCGTCG ACGGGCGCGG CGCGCCGCTT GCCGACGTGC CGCACATGGG CATCTCCGCC TACCGGATCG GCCGGCAGGT CGGCGACGCG ATCGCCGCCG AGGCGAAGCG GCGCGGCTGG AATCCGGCCG AGGTCGGCGT GCTGCGGCTC GCGTACGACC AGTTGCCGAC CGCGCGCGAG CGCACGACGG GCGCGGTCGA CGCGCTGAAG GCGGCCGGCT TTGCGGCCGC GAACGTCGTC GACGCGCCGG AGATGACGGC CGACACCGAA GGCGCGTTCA ACGCCGCGAA CATCGCGTTC ACCAAGCACC GGAACTTCAG GCACTGGGTG GCGTTCGGAT CGAATGACGA CACGACGGTC GGCGCGGTGC GCGCGGGCGA AGGCCGCGGC ATCGGCACGG ACGACATGAT CGCGGTCGGC ATCAACGGCA GCCAGGTCGC GCTGAACGAA TTCGCGAAAC CGAAGCCGAC GGGCTTTTTC GGCTCGATCC TGCTGAATCC GCGGCTGCAC GGCTACGACA CGTCGGTCAA CATGTACGAC TGGATCACGC AGAACCGGAC GCCGCCGCCG CTCGTGCTGA CCTCCGGCAC GCTGATCACG CGCGCGAACG AGAAGACGGC GCGCGCGCAG CTCGGGCTG
Details for ButhA.00100.b.B2.GE36103
| HARVESTED ON: | 9/26/2012 |
| SEQUENCED ON: | 9/25/2012 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 86 |
| PERCENT COVERAGE: | 104 |
Validated AA Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALXAAGFAAA NVVDAPEMTA DTEXAFNAAN IAFTKHRNFX HWVAFGSNDD TTVGAVRAGE XRGIGTDDMI AVGINGSQVA LNEFAKPKPX GFXXSILLNP XCXGYXXXXX XXXTXITXXX XXXXVLTXHA DHARDXXXXX QLXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttnntnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggcg caaggcgcgg ccccggtgaa gatcggcttt gtcgtcaagc agcccgacga cccgtggttt caggacgaat ggcgcttcgc cgagcaggcg gcgaaggaca agcacttcac gctcgtgaag atcgccgcgc cgagcggcga gaaagtgtcg accgcgctcg acagccttgc cgcgcaaaag gcgcaaggcg tgatcatctg cgcgcccgac gtgaagctcg gtcccggcat cgccgcgaag gcgaagcgct acggaatgaa gctgatgtcg gtcgacgatc aactcgtcga cgggcgcggc gcgccgcttg ccgacgtgcc gcacatgggc atctccgcct accggatcgg ccggcaggtc ggcgacgcga tcgccgccga ggcgaagcgg cgcggctgga atccggccga ggtcggcgtg ctgcggctcg cgtacgacca gttgccgacc gcgcgcgagc gcacgacggg cgcggtcgac gcgctgaang cggccggctt tgcggccgcg aacgtcgtcg acgcgccgga gatgacggcc gacaccgaan gcgcgttcaa cgccgcgaac atcgcgttca ccaagcaccg gaacttcang cactgggtgg cgttcggatc gaatgacgac acgacggtcg gcgcggtgcg cgcgggcgaa ngccgcggca tcggcacgga cgacatgatc gcggtcggca tcaacggcag ccaggtcgcg ctgaacgaat tcgcgaaacc gaagccgann ggctttttnn nntcgatcct gctgaatccn ngctgcnncg gctacnannn nnnngntcan nnnnnnncna ctnggatcac ncannnnnga cncnncncnn tcgtgctgac tnnncacgct gatcacgcgc gcgacganan annnnncncg cagctngnnt gnantganta nnnnnntnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnaa aa
Expected Protein Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALKAAGFAAA NVVDAPEMTA DTEGAFNAAN IAFTKHRNFR HWVAFGSNDD TTVGAVRAGE GRGIGTDDMI AVGINGSQVA LNEFAKPKPT GFFGSILLNP RLHGYDTSVN MYDWITQNRT PPPLVLTSGT LITRANEKTA RAQLGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aaggcgcggc cccggtgaag atcggctttg tcgtcaagca gcccgacgac ccgtggtttc aggacgaatg gcgcttcgcc gagcaggcgg cgaaggacaa gcacttcacg ctcgtgaaga tcgccgcgcc gagcggcgag aaagtgtcga ccgcgctcga cagccttgcc gcgcaaaagg cgcaaggcgt gatcatctgc gcgcccgacg tgaagctcgg tcccggcatc gccgcgaagg cgaagcgcta cggaatgaag ctgatgtcgg tcgacgatca actcgtcgac gggcgcggcg cgccgcttgc cgacgtgccg cacatgggca tctccgccta ccggatcggc cggcaggtcg gcgacgcgat cgccgccgag gcgaagcggc gcggctggaa tccggccgag gtcggcgtgc tgcggctcgc gtacgaccag ttgccgaccg cgcgcgagcg cacgacgggc gcggtcgacg cgctgaaggc ggccggcttt gcggccgcga acgtcgtcga cgcgccggag atgacggccg acaccgaagg cgcgttcaac gccgcgaaca tcgcgttcac caagcaccgg aacttcaggc actgggtggc gttcggatcg aatgacgaca cgacggtcgg cgcggtgcgc gcgggcgaag gccgcggcat cggcacggac gacatgatcg cggtcggcat caacggcagc caggtcgcgc tgaacgaatt cgcgaaaccg aagccgacgg gctttttcgg ctcgatcctg ctgaatccgc ggctgcacgg ctacgacacg tcggtcaaca tgtacgactg gatcacgcag aaccggacgc cgccgccgct cgtgctgacc tccggcacgc tgatcacgcg cgcgaacgag aagacggcgc gcgcgcagct cgggctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for ButhA.00100.b.B2.PS01715
| PURIFICATION DATe: | 12/18/2012 |
| CONCENTRATION: | 59.2mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 86 |
| PERCENT COVERAGE: | 104 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALXAAGFAAA NVVDAPEMTA DTEXAFNAAN IAFTKHRNFX HWVAFGSNDD TTVGAVRAGE XRGIGTDDMI AVGINGSQVA LNEFAKPKPX GFXXSILLNP XCXGYXXXXX XXXTXITXXX XXXXVLTXHA DHARDXXXXX QLXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttnntnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggcg caaggcgcgg ccccggtgaa gatcggcttt gtcgtcaagc agcccgacga cccgtggttt caggacgaat ggcgcttcgc cgagcaggcg gcgaaggaca agcacttcac gctcgtgaag atcgccgcgc cgagcggcga gaaagtgtcg accgcgctcg acagccttgc cgcgcaaaag gcgcaaggcg tgatcatctg cgcgcccgac gtgaagctcg gtcccggcat cgccgcgaag gcgaagcgct acggaatgaa gctgatgtcg gtcgacgatc aactcgtcga cgggcgcggc gcgccgcttg ccgacgtgcc gcacatgggc atctccgcct accggatcgg ccggcaggtc ggcgacgcga tcgccgccga ggcgaagcgg cgcggctgga atccggccga ggtcggcgtg ctgcggctcg cgtacgacca gttgccgacc gcgcgcgagc gcacgacggg cgcggtcgac gcgctgaang cggccggctt tgcggccgcg aacgtcgtcg acgcgccgga gatgacggcc gacaccgaan gcgcgttcaa cgccgcgaac atcgcgttca ccaagcaccg gaacttcang cactgggtgg cgttcggatc gaatgacgac acgacggtcg gcgcggtgcg cgcgggcgaa ngccgcggca tcggcacgga cgacatgatc gcggtcggca tcaacggcag ccaggtcgcg ctgaacgaat tcgcgaaacc gaagccgann ggctttttnn nntcgatcct gctgaatccn ngctgcnncg gctacnannn nnnngntcan nnnnnnncna ctnggatcac ncannnnnga cncnncncnn tcgtgctgac tnnncacgct gatcacgcgc gcgacganan annnnncncg cagctngnnt gnantganta nnnnnntnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnaa aa
Expressed Protein Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALKAAGFAAA NVVDAPEMTA DTEGAFNAAN IAFTKHRNFR HWVAFGSNDD TTVGAVRAGE GRGIGTDDMI AVGINGSQVA LNEFAKPKPT GFFGSILLNP RLHGYDTSVN MYDWITQNRT PPPLVLTSGT LITRANEKTA RAQLGL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC AAGGCGCGGC CCCGGTGAAG ATCGGCTTTG TCGTCAAGCA GCCCGACGAC CCGTGGTTTC AGGACGAATG GCGCTTCGCC GAGCAGGCGG CGAAGGACAA GCACTTCACG CTCGTGAAGA TCGCCGCGCC GAGCGGCGAG AAAGTGTCGA CCGCGCTCGA CAGCCTTGCC GCGCAAAAGG CGCAAGGCGT GATCATCTGC GCGCCCGACG TGAAGCTCGG TCCCGGCATC GCCGCGAAGG CGAAGCGCTA CGGAATGAAG CTGATGTCGG TCGACGATCA ACTCGTCGAC GGGCGCGGCG CGCCGCTTGC CGACGTGCCG CACATGGGCA TCTCCGCCTA CCGGATCGGC CGGCAGGTCG GCGACGCGAT CGCCGCCGAG GCGAAGCGGC GCGGCTGGAA TCCGGCCGAG GTCGGCGTGC TGCGGCTCGC GTACGACCAG TTGCCGACCG CGCGCGAGCG CACGACGGGC GCGGTCGACG CGCTGAAGGC GGCCGGCTTT GCGGCCGCGA ACGTCGTCGA CGCGCCGGAG ATGACGGCCG ACACCGAAGG CGCGTTCAAC GCCGCGAACA TCGCGTTCAC CAAGCACCGG AACTTCAGGC ACTGGGTGGC GTTCGGATCG AATGACGACA CGACGGTCGG CGCGGTGCGC GCGGGCGAAG GCCGCGGCAT CGGCACGGAC GACATGATCG CGGTCGGCAT CAACGGCAGC CAGGTCGCGC TGAACGAATT CGCGAAACCG AAGCCGACGG GCTTTTTCGG CTCGATCCTG CTGAATCCGC GGCTGCACGG CTACGACACG TCGGTCAACA TGTACGACTG GATCACGCAG AACCGGACGC CGCCGCCGCT CGTGCTGACC TCCGGCACGC TGATCACGCG CGCGAACGAG AAGACGGCGC GCGCGCAGCT CGGGCTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for ButhA.00100.b.B2.PS01716
| PURIFICATION DATe: | 12/18/2012 |
| CONCENTRATION: | 53.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 86 |
| PERCENT COVERAGE: | 104 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALXAAGFAAA NVVDAPEMTA DTEXAFNAAN IAFTKHRNFX HWVAFGSNDD TTVGAVRAGE XRGIGTDDMI AVGINGSQVA LNEFAKPKPX GFXXSILLNP XCXGYXXXXX XXXTXITXXX XXXXVLTXHA DHARDXXXXX QLXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttnntnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggcg caaggcgcgg ccccggtgaa gatcggcttt gtcgtcaagc agcccgacga cccgtggttt caggacgaat ggcgcttcgc cgagcaggcg gcgaaggaca agcacttcac gctcgtgaag atcgccgcgc cgagcggcga gaaagtgtcg accgcgctcg acagccttgc cgcgcaaaag gcgcaaggcg tgatcatctg cgcgcccgac gtgaagctcg gtcccggcat cgccgcgaag gcgaagcgct acggaatgaa gctgatgtcg gtcgacgatc aactcgtcga cgggcgcggc gcgccgcttg ccgacgtgcc gcacatgggc atctccgcct accggatcgg ccggcaggtc ggcgacgcga tcgccgccga ggcgaagcgg cgcggctgga atccggccga ggtcggcgtg ctgcggctcg cgtacgacca gttgccgacc gcgcgcgagc gcacgacggg cgcggtcgac gcgctgaang cggccggctt tgcggccgcg aacgtcgtcg acgcgccgga gatgacggcc gacaccgaan gcgcgttcaa cgccgcgaac atcgcgttca ccaagcaccg gaacttcang cactgggtgg cgttcggatc gaatgacgac acgacggtcg gcgcggtgcg cgcgggcgaa ngccgcggca tcggcacgga cgacatgatc gcggtcggca tcaacggcag ccaggtcgcg ctgaacgaat tcgcgaaacc gaagccgann ggctttttnn nntcgatcct gctgaatccn ngctgcnncg gctacnannn nnnngntcan nnnnnnncna ctnggatcac ncannnnnga cncnncncnn tcgtgctgac tnnncacgct gatcacgcgc gcgacganan annnnncncg cagctngnnt gnantganta nnnnnntnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnaa aa
Expressed Protein Sequence
MAHHHHHHMA QGAAPVKIGF VVKQPDDPWF QDEWRFAEQA AKDKHFTLVK IAAPSGEKVS TALDSLAAQK AQGVIICAPD VKLGPGIAAK AKRYGMKLMS VDDQLVDGRG APLADVPHMG ISAYRIGRQV GDAIAAEAKR RGWNPAEVGV LRLAYDQLPT ARERTTGAVD ALKAAGFAAA NVVDAPEMTA DTEGAFNAAN IAFTKHRNFR HWVAFGSNDD TTVGAVRAGE GRGIGTDDMI AVGINGSQVA LNEFAKPKPT GFFGSILLNP RLHGYDTSVN MYDWITQNRT PPPLVLTSGT LITRANEKTA RAQLGL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC AAGGCGCGGC CCCGGTGAAG ATCGGCTTTG TCGTCAAGCA GCCCGACGAC CCGTGGTTTC AGGACGAATG GCGCTTCGCC GAGCAGGCGG CGAAGGACAA GCACTTCACG CTCGTGAAGA TCGCCGCGCC GAGCGGCGAG AAAGTGTCGA CCGCGCTCGA CAGCCTTGCC GCGCAAAAGG CGCAAGGCGT GATCATCTGC GCGCCCGACG TGAAGCTCGG TCCCGGCATC GCCGCGAAGG CGAAGCGCTA CGGAATGAAG CTGATGTCGG TCGACGATCA ACTCGTCGAC GGGCGCGGCG CGCCGCTTGC CGACGTGCCG CACATGGGCA TCTCCGCCTA CCGGATCGGC CGGCAGGTCG GCGACGCGAT CGCCGCCGAG GCGAAGCGGC GCGGCTGGAA TCCGGCCGAG GTCGGCGTGC TGCGGCTCGC GTACGACCAG TTGCCGACCG CGCGCGAGCG CACGACGGGC GCGGTCGACG CGCTGAAGGC GGCCGGCTTT GCGGCCGCGA ACGTCGTCGA CGCGCCGGAG ATGACGGCCG ACACCGAAGG CGCGTTCAAC GCCGCGAACA TCGCGTTCAC CAAGCACCGG AACTTCAGGC ACTGGGTGGC GTTCGGATCG AATGACGACA CGACGGTCGG CGCGGTGCGC GCGGGCGAAG GCCGCGGCAT CGGCACGGAC GACATGATCG CGGTCGGCAT CAACGGCAGC CAGGTCGCGC TGAACGAATT CGCGAAACCG AAGCCGACGG GCTTTTTCGG CTCGATCCTG CTGAATCCGC GGCTGCACGG CTACGACACG TCGGTCAACA TGTACGACTG GATCACGCAG AACCGGACGC CGCCGCCGCT CGTGCTGACC TCCGGCACGC TGATCACGCG CGCGAACGAG AAGACGGCGC GCGCGCAGCT CGGGCTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT