ButhA.00100.a

L-arabinose ABC transporter, periplasmic L-arabinose-binding protein

CENTER ID: ButhA.00100.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00100.a.B2.GE36102 Mature protein( ButhA.00100.a ) 36 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00100.a.B2.PS01676 Mature protein( ButhA.00100.a ) 36 340
ButhA.00100.a.B2.PS01677 Mature protein( ButhA.00100.a ) 36 340

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1797
RefSeq: YP_439822.1
UniProt: Q2T4S7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRRRTMTRT IRRLTLRAAF AALVIAPLAM PAATRADTPL KIGFLVKMPE QAWFINEQKA ATALGQKEGF SVVNIGTPDG EKVLAAIDNL GSQGAQGFVI CAPDVRLGPA IAARAKRYDM KFVTVDDQLV DSSGKPLANV PHLGMSATKI GNQVGQAIAD EMKKRGWKPE EVGALRVTNY ELPTAKLRTD GATQALLASG FKKENIFDAP QKTTDDEGGF SAAAPVLARH PNVKKWVIFA LNEETVLGAV RATEQLHIPA ADVIGVGING AGEAFAEFQK KEPTGFFGTI AVSSTNHGKQ SAQNLVDWIR SGKAPPADTQ TSGKLMTREN WQAVRAELGI
NT Sequence
ATGACAAGGA GACGCACCAT GACCCGCACG ATTCGCCGAC TGACCCTGCG CGCCGCGTTC GCGGCGCTCG TGATCGCGCC GCTCGCGATG CCCGCCGCGA CCCGCGCGGA CACGCCGCTG AAGATCGGTT TCCTCGTGAA GATGCCCGAG CAGGCGTGGT TCATCAACGA GCAGAAGGCC GCGACGGCGC TCGGCCAGAA GGAAGGCTTC TCGGTCGTCA ACATCGGCAC GCCGGACGGC GAGAAGGTGC TCGCGGCGAT CGACAACCTC GGCTCGCAGG GCGCGCAGGG CTTCGTGATC TGCGCGCCGG ACGTGCGTCT CGGGCCGGCG ATCGCCGCGC GCGCGAAACG CTACGACATG AAGTTCGTGA CCGTCGACGA CCAGCTCGTC GATTCGTCGG GCAAGCCGCT CGCGAACGTG CCGCACCTCG GCATGTCGGC GACGAAGATC GGCAACCAGG TTGGCCAGGC GATCGCCGAC GAGATGAAAA AGCGCGGCTG GAAGCCGGAG GAGGTCGGCG CGCTGCGCGT GACGAACTAC GAGCTGCCGA CCGCGAAGCT GCGCACCGAC GGCGCGACGC AGGCGCTGCT CGCGAGCGGC TTCAAGAAGG AGAACATCTT CGACGCGCCG CAGAAGACGA CCGACGACGA AGGCGGCTTC AGCGCGGCGG CGCCCGTGCT CGCGCGGCAT CCGAACGTGA AGAAGTGGGT GATCTTCGCG CTGAACGAGG AAACGGTGCT GGGCGCGGTG CGCGCGACCG AGCAGTTGCA CATTCCGGCG GCGGACGTGA TCGGCGTGGG CATCAACGGC GCGGGCGAGG CGTTCGCCGA ATTCCAGAAG AAGGAGCCGA CGGGCTTCTT CGGCACGATC GCGGTCAGCT CGACGAACCA CGGCAAGCAG AGCGCGCAGA ACCTCGTCGA CTGGATTCGC AGCGGCAAGG CGCCGCCCGC CGATACGCAG ACGAGCGGCA AGCTGATGAC GCGCGAGAAC TGGCAGGCGG TGCGCGCCGA GCTGGGCATC
Details for ButhA.00100.a.B2.GE36102
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Validated AA Sequence
MKFVTVDDQL VDSSGKPLAN VPHLGMSATK IGNQVGQAIA DEMKKRGWKP EEVGALRVTN YELPTAKLRT DGATQALLAS GFKKENIFDA PQKTTDDEGG FSAAAPVLAR HPNVKKWVIF ALNEETVLGA VRATEQLHIP AADVIGVGIN GAGEAFAEFQ KKEPTGFFGT IAVSSTNHGK QSAQNLVDWI RSGKAPPADT QTSGKLMTRE NWQAVRAELG I
Validated NT Sequence
tcttactcac ttagatgccc agctcggcgc gcaccgcctg ccagttctcg cgcgtcatca gcttgccgct cgtctgcgta tcggcgggcg gcgccttgcc gctgcgaatc cagtcgacga ggttctgcgc gctctgcttg ccgtggttcg tcgagctgac cgcgatcgtg ccgaagaagc ccgtcggctc cttcttctgg aattcggcga acgcctcgcc cgcgccgttg atgcccacgc cgatcacgtc cgccgccgga atgtgcaact gctcggtcgc gcgcaccgcg cccagcaccg tttcctcgtt cagcgcgaag atcacccact tcttcacgtt cggatgccgc gcgagcacgg gcgccgccgc gctgaagccg ccttcgtcgt cggtcgtctt ctgcggcgcg tcgaagatgt tctccttctt gaagccgctc gcgagcagcg cctgcgtcgc gccgtcggtg cgcagcttcg cggtcggcag ctcgtagttc gtcacgcgca gcgcgccgac ctcctccggc ttccagccgc gctttttcat ctcgtcggcg atcgcctggc caacctggtt gccgatcttc gtcgccgaca tgccgaggtg cggcacgttc gcgagcggct tgcccgacga atcgacgagc tggtcgtcga cggtcacgaa cttcatgtcg tagcgtttcg cgcgcgcggc gatcgccggc ccgagacgca cgtccggcgc gcagatcacg aagccctgcg cgccctgcga gccgaggttg tcgatcgccg cgagcacctt ctcgccgtcc ggcgtgccga tgttgacgac cgagaagcct tccttctggc cgagcgccgt cgcggccttc tgctcgttga tgancnacgn ctgctcngg
Expected Protein Sequence
MAHHHHHHMA DTPLKIGFLV KMPEQAWFIN EQKAATALGQ KEGFSVVNIG TPDGEKVLAA IDNLGSQGAQ GFVICAPDVR LGPAIAARAK RYDMKFVTVD DQLVDSSGKP LANVPHLGMS ATKIGNQVGQ AIADEMKKRG WKPEEVGALR VTNYELPTAK LRTDGATQAL LASGFKKENI FDAPQKTTDD EGGFSAAAPV LARHPNVKKW VIFALNEETV LGAVRATEQL HIPAADVIGV GINGAGEAFA EFQKKEPTGF FGTIAVSSTN HGKQSAQNLV DWIRSGKAPP ADTQTSGKLM TRENWQAVRA ELGI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg acacgccgct gaagatcggt ttcctcgtga agatgcccga gcaggcgtgg ttcatcaacg agcagaaggc cgcgacggcg ctcggccaga aggaaggctt ctcggtcgtc aacatcggca cgccggacgg cgagaaggtg ctcgcggcga tcgacaacct cggctcgcag ggcgcgcagg gcttcgtgat ctgcgcgccg gacgtgcgtc tcgggccggc gatcgccgcg cgcgcgaaac gctacgacat gaagttcgtg accgtcgacg accagctcgt cgattcgtcg ggcaagccgc tcgcgaacgt gccgcacctc ggcatgtcgg cgacgaagat cggcaaccag gttggccagg cgatcgccga cgagatgaaa aagcgcggct ggaagccgga ggaggtcggc gcgctgcgcg tgacgaacta cgagctgccg accgcgaagc tgcgcaccga cggcgcgacg caggcgctgc tcgcgagcgg cttcaagaag gagaacatct tcgacgcgcc gcagaagacg accgacgacg aaggcggctt cagcgcggcg gcgcccgtgc tcgcgcggca tccgaacgtg aagaagtggg tgatcttcgc gctgaacgag gaaacggtgc tgggcgcggt gcgcgcgacc gagcagttgc acattccggc ggcggacgtg atcggcgtgg gcatcaacgg cgcgggcgag gcgttcgccg aattccagaa gaaggagccg acgggcttct tcggcacgat cgcggtcagc tcgacgaacc acggcaagca gagcgcgcag aacctcgtcg actggattcg cagcggcaag gcgccgcccg ccgatacgca gacgagcggc aagctgatga cgcgcgagaa ctggcaggcg gtgcgcgccg agctgggcat ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ButhA.00100.a.B2.PS01676
PURIFICATION DATe: 11/20/2012
CONCENTRATION: 54.3mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MKFVTVDDQL VDSSGKPLAN VPHLGMSATK IGNQVGQAIA DEMKKRGWKP EEVGALRVTN YELPTAKLRT DGATQALLAS GFKKENIFDA PQKTTDDEGG FSAAAPVLAR HPNVKKWVIF ALNEETVLGA VRATEQLHIP AADVIGVGIN GAGEAFAEFQ KKEPTGFFGT IAVSSTNHGK QSAQNLVDWI RSGKAPPADT QTSGKLMTRE NWQAVRAELG I
Validated NT Sequence
tcttactcac ttagatgccc agctcggcgc gcaccgcctg ccagttctcg cgcgtcatca gcttgccgct cgtctgcgta tcggcgggcg gcgccttgcc gctgcgaatc cagtcgacga ggttctgcgc gctctgcttg ccgtggttcg tcgagctgac cgcgatcgtg ccgaagaagc ccgtcggctc cttcttctgg aattcggcga acgcctcgcc cgcgccgttg atgcccacgc cgatcacgtc cgccgccgga atgtgcaact gctcggtcgc gcgcaccgcg cccagcaccg tttcctcgtt cagcgcgaag atcacccact tcttcacgtt cggatgccgc gcgagcacgg gcgccgccgc gctgaagccg ccttcgtcgt cggtcgtctt ctgcggcgcg tcgaagatgt tctccttctt gaagccgctc gcgagcagcg cctgcgtcgc gccgtcggtg cgcagcttcg cggtcggcag ctcgtagttc gtcacgcgca gcgcgccgac ctcctccggc ttccagccgc gctttttcat ctcgtcggcg atcgcctggc caacctggtt gccgatcttc gtcgccgaca tgccgaggtg cggcacgttc gcgagcggct tgcccgacga atcgacgagc tggtcgtcga cggtcacgaa cttcatgtcg tagcgtttcg cgcgcgcggc gatcgccggc ccgagacgca cgtccggcgc gcagatcacg aagccctgcg cgccctgcga gccgaggttg tcgatcgccg cgagcacctt ctcgccgtcc ggcgtgccga tgttgacgac cgagaagcct tccttctggc cgagcgccgt cgcggccttc tgctcgttga tgancnacgn ctgctcngg
Expressed Protein Sequence
MAHHHHHHMA DTPLKIGFLV KMPEQAWFIN EQKAATALGQ KEGFSVVNIG TPDGEKVLAA IDNLGSQGAQ GFVICAPDVR LGPAIAARAK RYDMKFVTVD DQLVDSSGKP LANVPHLGMS ATKIGNQVGQ AIADEMKKRG WKPEEVGALR VTNYELPTAK LRTDGATQAL LASGFKKENI FDAPQKTTDD EGGFSAAAPV LARHPNVKKW VIFALNEETV LGAVRATEQL HIPAADVIGV GINGAGEAFA EFQKKEPTGF FGTIAVSSTN HGKQSAQNLV DWIRSGKAPP ADTQTSGKLM TRENWQAVRA ELGI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG ACACGCCGCT GAAGATCGGT TTCCTCGTGA AGATGCCCGA GCAGGCGTGG TTCATCAACG AGCAGAAGGC CGCGACGGCG CTCGGCCAGA AGGAAGGCTT CTCGGTCGTC AACATCGGCA CGCCGGACGG CGAGAAGGTG CTCGCGGCGA TCGACAACCT CGGCTCGCAG GGCGCGCAGG GCTTCGTGAT CTGCGCGCCG GACGTGCGTC TCGGGCCGGC GATCGCCGCG CGCGCGAAAC GCTACGACAT GAAGTTCGTG ACCGTCGACG ACCAGCTCGT CGATTCGTCG GGCAAGCCGC TCGCGAACGT GCCGCACCTC GGCATGTCGG CGACGAAGAT CGGCAACCAG GTTGGCCAGG CGATCGCCGA CGAGATGAAA AAGCGCGGCT GGAAGCCGGA GGAGGTCGGC GCGCTGCGCG TGACGAACTA CGAGCTGCCG ACCGCGAAGC TGCGCACCGA CGGCGCGACG CAGGCGCTGC TCGCGAGCGG CTTCAAGAAG GAGAACATCT TCGACGCGCC GCAGAAGACG ACCGACGACG AAGGCGGCTT CAGCGCGGCG GCGCCCGTGC TCGCGCGGCA TCCGAACGTG AAGAAGTGGG TGATCTTCGC GCTGAACGAG GAAACGGTGC TGGGCGCGGT GCGCGCGACC GAGCAGTTGC ACATTCCGGC GGCGGACGTG ATCGGCGTGG GCATCAACGG CGCGGGCGAG GCGTTCGCCG AATTCCAGAA GAAGGAGCCG ACGGGCTTCT TCGGCACGAT CGCGGTCAGC TCGACGAACC ACGGCAAGCA GAGCGCGCAG AACCTCGTCG ACTGGATTCG CAGCGGCAAG GCGCCGCCCG CCGATACGCA GACGAGCGGC AAGCTGATGA CGCGCGAGAA CTGGCAGGCG GTGCGCGCCG AGCTGGGCAT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for ButhA.00100.a.B2.PS01677
PURIFICATION DATe: 11/20/2012
CONCENTRATION: 125.6mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MKFVTVDDQL VDSSGKPLAN VPHLGMSATK IGNQVGQAIA DEMKKRGWKP EEVGALRVTN YELPTAKLRT DGATQALLAS GFKKENIFDA PQKTTDDEGG FSAAAPVLAR HPNVKKWVIF ALNEETVLGA VRATEQLHIP AADVIGVGIN GAGEAFAEFQ KKEPTGFFGT IAVSSTNHGK QSAQNLVDWI RSGKAPPADT QTSGKLMTRE NWQAVRAELG I
Validated NT Sequence
tcttactcac ttagatgccc agctcggcgc gcaccgcctg ccagttctcg cgcgtcatca gcttgccgct cgtctgcgta tcggcgggcg gcgccttgcc gctgcgaatc cagtcgacga ggttctgcgc gctctgcttg ccgtggttcg tcgagctgac cgcgatcgtg ccgaagaagc ccgtcggctc cttcttctgg aattcggcga acgcctcgcc cgcgccgttg atgcccacgc cgatcacgtc cgccgccgga atgtgcaact gctcggtcgc gcgcaccgcg cccagcaccg tttcctcgtt cagcgcgaag atcacccact tcttcacgtt cggatgccgc gcgagcacgg gcgccgccgc gctgaagccg ccttcgtcgt cggtcgtctt ctgcggcgcg tcgaagatgt tctccttctt gaagccgctc gcgagcagcg cctgcgtcgc gccgtcggtg cgcagcttcg cggtcggcag ctcgtagttc gtcacgcgca gcgcgccgac ctcctccggc ttccagccgc gctttttcat ctcgtcggcg atcgcctggc caacctggtt gccgatcttc gtcgccgaca tgccgaggtg cggcacgttc gcgagcggct tgcccgacga atcgacgagc tggtcgtcga cggtcacgaa cttcatgtcg tagcgtttcg cgcgcgcggc gatcgccggc ccgagacgca cgtccggcgc gcagatcacg aagccctgcg cgccctgcga gccgaggttg tcgatcgccg cgagcacctt ctcgccgtcc ggcgtgccga tgttgacgac cgagaagcct tccttctggc cgagcgccgt cgcggccttc tgctcgttga tgancnacgn ctgctcngg
Expressed Protein Sequence
MAHHHHHHMA DTPLKIGFLV KMPEQAWFIN EQKAATALGQ KEGFSVVNIG TPDGEKVLAA IDNLGSQGAQ GFVICAPDVR LGPAIAARAK RYDMKFVTVD DQLVDSSGKP LANVPHLGMS ATKIGNQVGQ AIADEMKKRG WKPEEVGALR VTNYELPTAK LRTDGATQAL LASGFKKENI FDAPQKTTDD EGGFSAAAPV LARHPNVKKW VIFALNEETV LGAVRATEQL HIPAADVIGV GINGAGEAFA EFQKKEPTGF FGTIAVSSTN HGKQSAQNLV DWIRSGKAPP ADTQTSGKLM TRENWQAVRA ELGI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG ACACGCCGCT GAAGATCGGT TTCCTCGTGA AGATGCCCGA GCAGGCGTGG TTCATCAACG AGCAGAAGGC CGCGACGGCG CTCGGCCAGA AGGAAGGCTT CTCGGTCGTC AACATCGGCA CGCCGGACGG CGAGAAGGTG CTCGCGGCGA TCGACAACCT CGGCTCGCAG GGCGCGCAGG GCTTCGTGAT CTGCGCGCCG GACGTGCGTC TCGGGCCGGC GATCGCCGCG CGCGCGAAAC GCTACGACAT GAAGTTCGTG ACCGTCGACG ACCAGCTCGT CGATTCGTCG GGCAAGCCGC TCGCGAACGT GCCGCACCTC GGCATGTCGG CGACGAAGAT CGGCAACCAG GTTGGCCAGG CGATCGCCGA CGAGATGAAA AAGCGCGGCT GGAAGCCGGA GGAGGTCGGC GCGCTGCGCG TGACGAACTA CGAGCTGCCG ACCGCGAAGC TGCGCACCGA CGGCGCGACG CAGGCGCTGC TCGCGAGCGG CTTCAAGAAG GAGAACATCT TCGACGCGCC GCAGAAGACG ACCGACGACG AAGGCGGCTT CAGCGCGGCG GCGCCCGTGC TCGCGCGGCA TCCGAACGTG AAGAAGTGGG TGATCTTCGC GCTGAACGAG GAAACGGTGC TGGGCGCGGT GCGCGCGACC GAGCAGTTGC ACATTCCGGC GGCGGACGTG ATCGGCGTGG GCATCAACGG CGCGGGCGAG GCGTTCGCCG AATTCCAGAA GAAGGAGCCG ACGGGCTTCT TCGGCACGAT CGCGGTCAGC TCGACGAACC ACGGCAAGCA GAGCGCGCAG AACCTCGTCG ACTGGATTCG CAGCGGCAAG GCGCCGCCCG CCGATACGCA GACGAGCGGC AAGCTGATGA CGCGCGAGAA CTGGCAGGCG GTGCGCGCCG AGCTGGGCAT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT