ButhA.00097.a
Pantothenate synthetase (PS) (EC 6.3.2.1) (Pantoate--beta-alanine ligase) (Pantoate-activating enzyme) (BTH_I0848) (panC)
| CENTER ID: | ButhA.00097.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00097.a.A1.GE32719 | full length | 1 | 279 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00097.a.A1.PS01165 | full length | 1 | 279 |
Structures
3UK2
DEPOSITED: 11/8/2011
DETERMINATION: XRay
CLONE: ButhA.00097.a.A1.GE32719
PROTEIN: ButhA.00097.a.A1.PS01165
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129569 |
| PATRIC ID: | fig|271848.6.peg.3568 |
| RefSeq: | YP_441404.1 |
| UniProt: | Q2T095 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVISSIQEL RDQLRGQNRT AFVPTMGNLH EGHLSLMRLA RQHGDPVVAS IFVNRLQFGP NEDFDKYPRT LQEDIEKLQK ENVYVLFAPT ERDMYPEPQE YRVQPPHDLG DILEGEFRPG FFTGVCTVVT KLMACVQPRV AVFGKKDYQQ LMIVRRMCQQ LALPVEIVAA ETVRDADGLA LSSRNRYLSE AERAEAPELA KTLARVRDAV LDGERDLAAI ERRAVAHLSA RGWQPDYVSI RRRENLVAPS AAQIEAGDPL VVLTAAKLGA TRLIDNLEI
NT Sequence
atgaaagtca tcagctcgat ccaggaactg cgcgaccagt tgcgcggcca gaaccgcacg gcgttcgtgc cgacgatggg caacctgcac gaggggcacc tgtcgctgat gcggctcgcg cgccagcacg gcgatccggt cgtcgcgagc atcttcgtga accggctcca gttcggtccg aacgaggact tcgacaaata cccgcgcacg ctgcaggaag acatcgagaa gctgcagaag gaaaacgtct acgtgctgtt cgcgccgacc gagcgcgaca tgtaccccga gccgcaggaa tatcgcgtgc agccgccgca cgacctcggc gacatcctcg aaggcgagtt ccgccccggc ttcttcacgg gcgtgtgcac ggtcgtcacg aagctgatgg cgtgcgtgca gccgcgcgtc gccgtgttcg gcaagaagga ttaccagcag ttgatgatcg tgcgtcggat gtgccagcag ctcgcgctgc ccgtcgagat cgtcgcggcc gaaaccgtgc gcgacgccga cggcctcgcg ctgtcgtcgc gcaaccgcta tctgagcgaa gccgagcgcg cggaggcgcc cgagctcgcg aagacgctcg cgcgggtgcg cgacgcggtg ctcgacggcg agcgcgatct cgcggcgatc gagcggcgcg cggtcgcgca cctctccgcg cgcggctggc agcccgacta cgtgtcgatc cgccgacgcg agaacctcgt cgcgccgagc gcggcgcaaa tcgaagcggg cgatccgctc gtcgtgctca cggcggcgaa gctcggcgcg acgcgcctca tcgacaatct ggaaatctga
Details for ButhA.00097.a.A1.GE32719
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVISSIQE LRDQLRGQNR TAFVPTMGNL HEGHLSLMRL ARQHGDPVVA SIFVNRLQFG PNEDFDKYPR TLQEDIEKLQ KENVYVLFAP TERDMYPEPQ EYRVQPPHDL GDILEGEFRP GFFTGVCTVV TKLMACVQPR VAVFGKKDYQ QLMIVRRMCQ QLALPVEIVA AETVRDADGL ALSSRNRYLS EAERAEAPEL AKTLARVRDA VLDGERDLAA IERRAVAHLS ARGWQPDYVS IRRRENLVAP SAAQIEAGDP LVVLTAAKLG ATRLIDNL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag tcatcagctc gatccaggaa ctgcgcgacc agttgcgcgg ccagaaccgc acggcgttcg tgccgacgat gggcaacctg cacgaggggc acctgtcgct gatgcggctc gcgcgccagc acggcgatcc ggtcgtcgcg agcatcttcg tgaaccggct ccagttcggt ccgaacgagg acttcgacaa atacccgcgc acgctgcagg aagacatcga gaagctgcag aaggaaaacg tctacgtgct gttcgcgccg accgagcgcg acatgtaccc cgagccgcag gaatatcgcg tgcagccgcc gcacgacctc ggcgacatcc tcgaaggcga gttccgcccc ggcttcttca cgggcgtgtg cacggtcgtc acgaagctga tggcgtgcgt gcagccgcgc gtcgccgtgt tcggcaagaa ggattaccag cagttgatga tcgtgcgtcg gatgtgccag cagctcgcgc tgcccgtcga gatcgtcgcg gccgaaaccg tgcgcgacgc cgacggcctc gcgctgtcgt cgcgcaaccg ctatctgagc gaagccgagc gcgcggaggc gcccgagctc gcgaagacgc tcgcgcgggt gcgcgacgcg gtgctcgacg gcgagcgcga tctcgcggcg atcgagcggc gcgcggtcgc gcacctctcc gcgcgcggct ggcagcccga ctacgtgtcg atccgccgac gcgagaacct cgtcgcgccg agcgcggcgc aaatcgaagc gggcgatccg ctcgtcgtgc tcacggcggc gaagctcggc gcgacgcgcc tcatcgacaa tctgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVISSIQE LRDQLRGQNR TAFVPTMGNL HEGHLSLMRL ARQHGDPVVA SIFVNRLQFG PNEDFDKYPR TLQEDIEKLQ KENVYVLFAP TERDMYPEPQ EYRVQPPHDL GDILEGEFRP GFFTGVCTVV TKLMACVQPR VAVFGKKDYQ QLMIVRRMCQ QLALPVEIVA AETVRDADGL ALSSRNRYLS EAERAEAPEL AKTLARVRDA VLDGERDLAA IERRAVAHLS ARGWQPDYVS IRRRENLVAP SAAQIEAGDP LVVLTAAKLG ATRLIDNLEI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagtcatc agctcgatcc aggaactgcg cgaccagttg cgcggccaga accgcacggc gttcgtgccg acgatgggca acctgcacga ggggcacctg tcgctgatgc ggctcgcgcg ccagcacggc gatccggtcg tcgcgagcat cttcgtgaac cggctccagt tcggtccgaa cgaggacttc gacaaatacc cgcgcacgct gcaggaagac atcgagaagc tgcagaagga aaacgtctac gtgctgttcg cgccgaccga gcgcgacatg taccccgagc cgcaggaata tcgcgtgcag ccgccgcacg acctcggcga catcctcgaa ggcgagttcc gccccggctt cttcacgggc gtgtgcacgg tcgtcacgaa gctgatggcg tgcgtgcagc cgcgcgtcgc cgtgttcggc aagaaggatt accagcagtt gatgatcgtg cgtcggatgt gccagcagct cgcgctgccc gtcgagatcg tcgcggccga aaccgtgcgc gacgccgacg gcctcgcgct gtcgtcgcgc aaccgctatc tgagcgaagc cgagcgcgcg gaggcgcccg agctcgcgaa gacgctcgcg cgggtgcgcg acgcggtgct cgacggcgag cgcgatctcg cggcgatcga gcggcgcgcg gtcgcgcacc tctccgcgcg cggctggcag cccgactacg tgtcgatccg ccgacgcgag aacctcgtcg cgccgagcgc ggcgcaaatc gaagcgggcg atccgctcgt cgtgctcacg gcggcgaagc tcggcgcgac gcgcctcatc gacaatctgg aaatctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.00097.a.A1.PS01165
| PURIFICATION DATe: | 8/9/2011 |
| CONCENTRATION: | 51.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVISSIQE LRDQLRGQNR TAFVPTMGNL HEGHLSLMRL ARQHGDPVVA SIFVNRLQFG PNEDFDKYPR TLQEDIEKLQ KENVYVLFAP TERDMYPEPQ EYRVQPPHDL GDILEGEFRP GFFTGVCTVV TKLMACVQPR VAVFGKKDYQ QLMIVRRMCQ QLALPVEIVA AETVRDADGL ALSSRNRYLS EAERAEAPEL AKTLARVRDA VLDGERDLAA IERRAVAHLS ARGWQPDYVS IRRRENLVAP SAAQIEAGDP LVVLTAAKLG ATRLIDNL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag tcatcagctc gatccaggaa ctgcgcgacc agttgcgcgg ccagaaccgc acggcgttcg tgccgacgat gggcaacctg cacgaggggc acctgtcgct gatgcggctc gcgcgccagc acggcgatcc ggtcgtcgcg agcatcttcg tgaaccggct ccagttcggt ccgaacgagg acttcgacaa atacccgcgc acgctgcagg aagacatcga gaagctgcag aaggaaaacg tctacgtgct gttcgcgccg accgagcgcg acatgtaccc cgagccgcag gaatatcgcg tgcagccgcc gcacgacctc ggcgacatcc tcgaaggcga gttccgcccc ggcttcttca cgggcgtgtg cacggtcgtc acgaagctga tggcgtgcgt gcagccgcgc gtcgccgtgt tcggcaagaa ggattaccag cagttgatga tcgtgcgtcg gatgtgccag cagctcgcgc tgcccgtcga gatcgtcgcg gccgaaaccg tgcgcgacgc cgacggcctc gcgctgtcgt cgcgcaaccg ctatctgagc gaagccgagc gcgcggaggc gcccgagctc gcgaagacgc tcgcgcgggt gcgcgacgcg gtgctcgacg gcgagcgcga tctcgcggcg atcgagcggc gcgcggtcgc gcacctctcc gcgcgcggct ggcagcccga ctacgtgtcg atccgccgac gcgagaacct cgtcgcgccg agcgcggcgc aaatcgaagc gggcgatccg ctcgtcgtgc tcacggcggc gaagctcggc gcgacgcgcc tcatcgacaa tctgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVISSIQE LRDQLRGQNR TAFVPTMGNL HEGHLSLMRL ARQHGDPVVA SIFVNRLQFG PNEDFDKYPR TLQEDIEKLQ KENVYVLFAP TERDMYPEPQ EYRVQPPHDL GDILEGEFRP GFFTGVCTVV TKLMACVQPR VAVFGKKDYQ QLMIVRRMCQ QLALPVEIVA AETVRDADGL ALSSRNRYLS EAERAEAPEL AKTLARVRDA VLDGERDLAA IERRAVAHLS ARGWQPDYVS IRRRENLVAP SAAQIEAGDP LVVLTAAKLG ATRLIDNLEI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGTCATC AGCTCGATCC AGGAACTGCG CGACCAGTTG CGCGGCCAGA ACCGCACGGC GTTCGTGCCG ACGATGGGCA ACCTGCACGA GGGGCACCTG TCGCTGATGC GGCTCGCGCG CCAGCACGGC GATCCGGTCG TCGCGAGCAT CTTCGTGAAC CGGCTCCAGT TCGGTCCGAA CGAGGACTTC GACAAATACC CGCGCACGCT GCAGGAAGAC ATCGAGAAGC TGCAGAAGGA AAACGTCTAC GTGCTGTTCG CGCCGACCGA GCGCGACATG TACCCCGAGC CGCAGGAATA TCGCGTGCAG CCGCCGCACG ACCTCGGCGA CATCCTCGAA GGCGAGTTCC GCCCCGGCTT CTTCACGGGC GTGTGCACGG TCGTCACGAA GCTGATGGCG TGCGTGCAGC CGCGCGTCGC CGTGTTCGGC AAGAAGGATT ACCAGCAGTT GATGATCGTG CGTCGGATGT GCCAGCAGCT CGCGCTGCCC GTCGAGATCG TCGCGGCCGA AACCGTGCGC GACGCCGACG GCCTCGCGCT GTCGTCGCGC AACCGCTATC TGAGCGAAGC CGAGCGCGCG GAGGCGCCCG AGCTCGCGAA GACGCTCGCG CGGGTGCGCG ACGCGGTGCT CGACGGCGAG CGCGATCTCG CGGCGATCGA GCGGCGCGCG GTCGCGCACC TCTCCGCGCG CGGCTGGCAG CCCGACTACG TGTCGATCCG CCGACGCGAG AACCTCGTCG CGCCGAGCGC GGCGCAAATC GAAGCGGGCG ATCCGCTCGT CGTGCTCACG GCGGCGAAGC TCGGCGCGAC GCGCCTCATC GACAATCTGG AAATCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA