SSGCID: Target Page

ButhA.00095.a

Hydrolase, alpha/beta fold family

CENTER ID:	ButhA.00095.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00095.a.B1.GE36100	Full length( ButhA.00095.a )		1	271

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00095.a.B1.PS01717	Full length( ButhA.00095.a )		1	271

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.6098
RefSeq:	YP_443701.1
UniProt:	Q2STP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPFVTIDGQP LHYQIRGSGA PVLFGHSYLW DSSMWEPQLE ALSKSYRVIA PDLWGHGQSG PLPDGTNDLD DLAMQMCVLL DHLGIDTCSI VGLSVGGMWA VPLAHRAPQR IDRLVLMDTY VGIEPDATRN KYFQMLAAID AQGAIPAPLL DAIVPIFFRP GIDLASELPA GFRRALQAFT TERLRDSVIP LGKITFGRED ARAKLSALPA QRTLVMCGAN DVARPPEEAD EIATLIGCEK VFVPNAGHIS NLENPAFVTQ ALIDWLGRGS N

NT Sequence

ATGCCTTTCG TCACTATCGA TGGCCAACCC CTGCACTATC AGATCAGGGG CTCCGGCGCG CCCGTGCTGT TCGGGCACAG CTACCTGTGG GATTCGTCGA TGTGGGAGCC GCAGCTCGAA GCGCTCTCGA AGTCGTATCG CGTGATCGCG CCGGACCTGT GGGGGCACGG CCAATCCGGC CCGCTGCCCG ACGGCACGAA CGATCTCGAC GATCTGGCGA TGCAGATGTG CGTGCTCCTC GATCATCTCG GCATCGACAC CTGCTCGATC GTCGGGCTGT CGGTGGGCGG CATGTGGGCG GTGCCGCTCG CGCACCGCGC GCCGCAGCGG ATCGACCGTC TCGTGCTGAT GGACACCTAT GTCGGCATCG AGCCCGACGC GACACGCAAC AAGTATTTCC AGATGCTCGC GGCCATCGAC GCGCAAGGCG CGATCCCCGC GCCGCTGCTC GACGCGATCG TGCCGATCTT CTTCCGCCCC GGCATCGATC TCGCGAGCGA GCTGCCTGCG GGCTTCCGGC GCGCGCTGCA GGCGTTCACG ACCGAGAGGC TGCGCGACTC GGTGATTCCG CTCGGCAAGA TCACGTTTGG CCGCGAAGAC GCACGCGCGA AACTCAGCGC GCTGCCGGCC CAGCGCACGC TCGTGATGTG CGGCGCGAAC GACGTCGCGC GGCCCCCCGA GGAAGCCGAC GAAATCGCAA CGCTGATCGG CTGCGAAAAG GTGTTCGTGC CGAACGCCGG GCACATTTCG AATCTCGAGA ACCCGGCGTT CGTGACGCAG GCGTTGATCG ATTGGCTCGG ACGCGGCAGC AAC

Details for ButhA.00095.a.B1.GE36100

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/25/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMP FVTIDGQPLH YQIRGSGAPV LFGHSYLWDS SMWEPQLEAL SKSYRVIAPD LWGHGQSGPL PDGTNDLDDL AMQMCVLLDH LGIDTCSIVG LSVGGMWAVP LAHRAPQRID RLVLMDTYVG IEPDATRNKY FQMLAAIDAQ GAIPAPLLDA IVPIFFRPGI DLASELPAGF RRALXAFTTE RLRDSVIPLG KITFGREDAR AKLSALPAQR TLVMCGANDV ARPPEEADEX ATLIGCEKVF VPNAXHISXX XXXXXXXXXX

Validated NT Sequence

ttnnnttact ttaagaagga ganntaccat ggctcaccac caccaccacc atatgccttt cgtcactatc gatggccaac ccctgcacta tcagatcagg ggctccggcg cgcccgtgct gttcgggcac agctacctgt gggattcgtc gatgtgggag ccgcagctcg aagcgctctc gaagtcgtat cgcgtgatcg cgccggacct gtgggggcac ggccaatccg gcccgctgcc cgacggcacg aacgatctcg acgatctggc gatgcagatg tgcgtgctcc tcgatcatct cggcatcgac acctgctcga tcgtcgggct gtcggtgggc ggcatgtggg cggtgccgct cgcgcaccgc gcgccgcagc ggatcgaccg tctcgtgctg atggacacct atgtcggcat cgagcccgac gcgacacgca acaagtattt ccagatgctc gcggccatcg acgcgcaagg cgcgatcccc gcgccgctgc tcgacgcgat cgtgccgatc ttcttccgcc ccggcatcga tctcgcgagc gagctgcctg cgggcttccg gcgcgcgctg cangcgttca cgaccgagag gctgcgcgac tcggtgattc cgctcggcaa gatcacgttt ggccgcgaag acgcacgcgc gaaactcagc gcgctgccgg cccagcgcac gctcgtgatg tgcggcgcga acgacgtcgc gcggcccccc gaggaagccg acgaantcgc aacgctgatc ggctgcgaaa aggtgttcgt gccgaacgcc nggcacattt cnaannnnnn nnnncnnnnn nnnnnnnnnn nnnggnnttg atcgattggc tcggacgcgg cagcaactaa gtgagtaana nnnntcnnnn nnncnncccc cnnnnnnnnn anaaaaaaaa ngnnngnnnn anntnnnnnn gcnnnntnnn nnnnttngcn gnnnnnnnnn nngnntnnnn nnnnnnngnn nnnannnnnn ncnnnnnnnn nnnnnnngnt nngnnntnnn nnnt

Expected Protein Sequence

MAHHHHHHMP FVTIDGQPLH YQIRGSGAPV LFGHSYLWDS SMWEPQLEAL SKSYRVIAPD LWGHGQSGPL PDGTNDLDDL AMQMCVLLDH LGIDTCSIVG LSVGGMWAVP LAHRAPQRID RLVLMDTYVG IEPDATRNKY FQMLAAIDAQ GAIPAPLLDA IVPIFFRPGI DLASELPAGF RRALQAFTTE RLRDSVIPLG KITFGREDAR AKLSALPAQR TLVMCGANDV ARPPEEADEI ATLIGCEKVF VPNAGHISNL ENPAFVTQAL IDWLGRGSN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctt tcgtcactat cgatggccaa cccctgcact atcagatcag gggctccggc gcgcccgtgc tgttcgggca cagctacctg tgggattcgt cgatgtggga gccgcagctc gaagcgctct cgaagtcgta tcgcgtgatc gcgccggacc tgtgggggca cggccaatcc ggcccgctgc ccgacggcac gaacgatctc gacgatctgg cgatgcagat gtgcgtgctc ctcgatcatc tcggcatcga cacctgctcg atcgtcgggc tgtcggtggg cggcatgtgg gcggtgccgc tcgcgcaccg cgcgccgcag cggatcgacc gtctcgtgct gatggacacc tatgtcggca tcgagcccga cgcgacacgc aacaagtatt tccagatgct cgcggccatc gacgcgcaag gcgcgatccc cgcgccgctg ctcgacgcga tcgtgccgat cttcttccgc cccggcatcg atctcgcgag cgagctgcct gcgggcttcc ggcgcgcgct gcaggcgttc acgaccgaga ggctgcgcga ctcggtgatt ccgctcggca agatcacgtt tggccgcgaa gacgcacgcg cgaaactcag cgcgctgccg gcccagcgca cgctcgtgat gtgcggcgcg aacgacgtcg cgcggccccc cgaggaagcc gacgaaatcg caacgctgat cggctgcgaa aaggtgttcg tgccgaacgc cgggcacatt tcgaatctcg agaacccggc gttcgtgacg caggcgttga tcgattggct cggacgcggc agcaactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for ButhA.00095.a.B1.PS01717

PURIFICATION DATe:	1/9/2012
CONCENTRATION:	67mg/ml
OBSERVED MW:	29kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMP FVTIDGQPLH YQIRGSGAPV LFGHSYLWDS SMWEPQLEAL SKSYRVIAPD LWGHGQSGPL PDGTNDLDDL AMQMCVLLDH LGIDTCSIVG LSVGGMWAVP LAHRAPQRID RLVLMDTYVG IEPDATRNKY FQMLAAIDAQ GAIPAPLLDA IVPIFFRPGI DLASELPAGF RRALQAFTTE RLRDSVIPLG KITFGREDAR AKLSALPAQR TLVMCGANDV ARPPEEADEI ATLIGCEKVF VPNAGHISNL ENPAFVTQAL IDWLGRGSN

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTT TCGTCACTAT CGATGGCCAA CCCCTGCACT ATCAGATCAG GGGCTCCGGC GCGCCCGTGC TGTTCGGGCA CAGCTACCTG TGGGATTCGT CGATGTGGGA GCCGCAGCTC GAAGCGCTCT CGAAGTCGTA TCGCGTGATC GCGCCGGACC TGTGGGGGCA CGGCCAATCC GGCCCGCTGC CCGACGGCAC GAACGATCTC GACGATCTGG CGATGCAGAT GTGCGTGCTC CTCGATCATC TCGGCATCGA CACCTGCTCG ATCGTCGGGC TGTCGGTGGG CGGCATGTGG GCGGTGCCGC TCGCGCACCG CGCGCCGCAG CGGATCGACC GTCTCGTGCT GATGGACACC TATGTCGGCA TCGAGCCCGA CGCGACACGC AACAAGTATT TCCAGATGCT CGCGGCCATC GACGCGCAAG GCGCGATCCC CGCGCCGCTG CTCGACGCGA TCGTGCCGAT CTTCTTCCGC CCCGGCATCG ATCTCGCGAG CGAGCTGCCT GCGGGCTTCC GGCGCGCGCT GCAGGCGTTC ACGACCGAGA GGCTGCGCGA CTCGGTGATT CCGCTCGGCA AGATCACGTT TGGCCGCGAA GACGCACGCG CGAAACTCAG CGCGCTGCCG GCCCAGCGCA CGCTCGTGAT GTGCGGCGCG AACGACGTCG CGCGGCCCCC CGAGGAAGCC GACGAAATCG CAACGCTGAT CGGCTGCGAA AAGGTGTTCG TGCCGAACGC CGGGCACATT TCGAATCTCG AGAACCCGGC GTTCGTGACG CAGGCGTTGA TCGATTGGCT CGGACGCGGC AGCAACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T