ButhA.00093.c

Oxidoreductase, FAD/FMN-binding

CENTER ID: ButhA.00093.c
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00093.c.B1.GE36099 Full length( ButhA.00093.c ) 1 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.906
RefSeq: YP_439000.1
UniProt: Q2T749

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDSNPAAAA LLAQPLTLPN GTVLKNRIAK SALSEALCGH DGRVTPQLIA LYRRWSNSGA GLLVTGNVMI DGKALGEPGN VVIEDERDLP RLREWAEAAK SHGSEIWMQI NHPGKQAMRG LNAETVAPSA IGFGPKLSPY FAVPRALTGD EIGELIRRYG TTAAVAQQAG FTGVQLHGAH GYLINQFLSP QHNQRTDEWG GSAENRRRFV LEVYAQVRRR VGPDFPIGIK LNSADFQRGG FTEEESLDVI RALAEAGIDL IEISGGTYET PVMQLGDRKA STVAREAYFL AFAEKVRAEV KVPLMVTGGF RSLAGMQAPL RDGALDLIGL GRILAIEPDA PARLLRGEET RHRVKPLSTG VKYLDSLGSL EVTWYTRQLH RIGKGRNPVP NENALKSFLL DLSSKGCAIF KARRLRASSS EASAAHGSAR SIAGGQRHDA H
NT Sequence
ATGTCCGATT CGAATCCCGC CGCGGCCGCG TTGCTCGCGC AGCCGCTCAC GCTGCCGAAC GGCACCGTGC TGAAGAACCG CATCGCGAAA TCGGCGCTGA GCGAAGCGCT GTGCGGTCAC GACGGCCGCG TGACGCCGCA GCTGATCGCG CTGTACCGGC GCTGGTCGAA TTCCGGCGCG GGGCTGCTCG TCACCGGCAA CGTGATGATC GACGGCAAGG CGCTCGGCGA GCCCGGCAAC GTCGTGATCG AGGACGAGCG TGACCTGCCG CGTCTGCGCG AATGGGCCGA GGCCGCGAAA TCGCACGGCA GCGAGATCTG GATGCAGATC AATCACCCGG GCAAGCAGGC GATGCGCGGC CTGAACGCGG AGACCGTCGC GCCGTCGGCG ATCGGCTTCG GGCCGAAGCT GTCGCCGTAT TTCGCGGTGC CGCGCGCGCT GACGGGCGAC GAGATCGGCG AGCTGATCCG GCGCTACGGA ACGACGGCCG CCGTCGCGCA GCAGGCGGGC TTCACCGGCG TGCAACTGCA CGGCGCGCAC GGCTATCTGA TCAACCAGTT CCTGTCGCCG CAGCACAATC AGCGCACCGA CGAATGGGGC GGCAGCGCGG AGAACCGCCG GCGCTTCGTG CTCGAGGTGT ACGCGCAAGT GCGGCGCCGG GTGGGCCCTG ATTTTCCGAT CGGCATCAAG CTCAATTCGG CCGACTTCCA GCGCGGCGGC TTCACCGAGG AAGAATCGCT CGACGTGATC CGCGCGCTCG CCGAAGCGGG CATCGATCTG ATCGAGATTT CCGGCGGCAC CTACGAAACG CCCGTGATGC AGCTCGGCGA CCGCAAGGCG TCGACTGTCG CGCGCGAGGC GTACTTCCTC GCATTCGCCG AGAAGGTGCG CGCCGAAGTG AAGGTGCCGC TGATGGTCAC GGGCGGCTTT CGCAGCCTCG CCGGCATGCA AGCGCCGCTG CGCGACGGCG CGCTCGACCT GATCGGCCTC GGGCGCATTC TCGCGATCGA GCCCGACGCG CCCGCCCGCC TGCTGCGCGG CGAGGAAACG CGGCATCGCG TGAAGCCGCT CAGCACGGGC GTCAAATACC TCGACAGTCT CGGTTCGCTC GAGGTCACGT GGTATACGCG CCAGCTCCAT CGAATCGGCA AGGGCCGCAA TCCGGTCCCC AACGAGAACG CGCTGAAGTC GTTCCTGCTC GATTTGTCGT CCAAGGGCTG CGCGATCTTC AAGGCGCGGC GCCTGCGTGC TTCGTCATCC GAAGCGTCGG CCGCACATGG CTCGGCGCGT TCGATCGCCG GCGGGCAGCG GCACGACGCG CAT
Details for ButhA.00093.c.B1.GE36099
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 48kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMS DSNPAAAALL AQPLTLPNGT VLKNRIAKSA LSEALCGHDG RVTPQLIALY RRWSNSGAGL LVTGNVMIDG KALGEPGNVV IEDERDLPRL REWAEAAKSH GSEIWMQINH PGKQAMRGLN AETVAPSAIG FGPKLSPYFA VPRALTGDEI GELIRRYGTT AAVAQXAGFT GVQLHGAHGY LINQFLSPQH NQRTDEWGGS AENRRRFVLE VYAQVRRXVG PDFPIGIKLN SADFQRGGFT EEESLDVIRA LAEAGIDLIE IXXGTYETPV MXLGXXKAST VARXXTSSHX PRXXRRXXXP LXVTGGFRSL XXCKRXXRXX XDLXXXGAXX XXXPXXPPXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXX
Validated NT Sequence
antttnntta ntttaagaag gaganntacc atggctcacc accaccacca ccatatgtcc gattcgaatc ccgccgcggc cgcgttgctc gcgcagccgc tcacgctgcc gaacggcacc gtgctgaaga accgcatcgc gaaatcggcg ctgagcgaag cgctgtgcgg tcacgacggc cgcgtgacgc cgcagctgat cgcgctgtac cggcgctggt cgaattccgg cgcggggctg ctcgtcaccg gcaacgtgat gatcgacggc aaggcgctcg gcgagcccgg caacgtcgtg atcgaggacg agcgtgacct gccgcgtctg cgcgaatggg ccgaggccgc gaaatcgcac ggcagcgaga tctggatgca gatcaatcac ccgggcaagc aggcgatgcg cggcctgaac gcggagaccg tcgcgccgtc ggcgatcggc ttcgggccga agctgtcgcc gtatttcgcg gtgccgcgcg cgctgacggg cgacgagatc ggcgagctga tccggcgcta cggaacgacg gccgccgtcg cgcagcangc gggcttcacc ggcgtgcaac tgcacggcgc gcacggctat ctgatcaacc agttcctgtc gccgcagcac aatcagcgca ccgacgaatg gggcggcagc gcggagaacc gccggcgctt cgtgctcgag gtgtacgcgc aagtgcggcg ccnggtgggc cctgattttc cgatcggcat caagctcaat tcggccgact tccagcgcgg cggcttcacc gaggaagaat cgctcgacgt gatccgcgcg ctcgccgaag cgggcatcga tctgatcgag atttncnnng gcacctacga aacgcccgtg atgcanctcg gcgannncaa ggcgtcgact gtcgcgcgcg annntacttc ctcgcattnn ccnagaanng nncgccgaan tgannngccn ctganggtca cgggcggctt tcgcagcctc nnnnnntgca agcgcnctnn ncgannnnnn nncgacctgn tnnncnnngg ngcatnnnnn nnatnnannc cnannnnncc nccnnncnnn nnnnnggnnn aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngnnn nnnnncnnnn nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn nnnnnnnnnn nannnnnnnn nncnnnnann nnnnnnnnnn nnnnnnntnn nnna
Expected Protein Sequence
MAHHHHHHMS DSNPAAAALL AQPLTLPNGT VLKNRIAKSA LSEALCGHDG RVTPQLIALY RRWSNSGAGL LVTGNVMIDG KALGEPGNVV IEDERDLPRL REWAEAAKSH GSEIWMQINH PGKQAMRGLN AETVAPSAIG FGPKLSPYFA VPRALTGDEI GELIRRYGTT AAVAQQAGFT GVQLHGAHGY LINQFLSPQH NQRTDEWGGS AENRRRFVLE VYAQVRRRVG PDFPIGIKLN SADFQRGGFT EEESLDVIRA LAEAGIDLIE ISGGTYETPV MQLGDRKAST VAREAYFLAF AEKVRAEVKV PLMVTGGFRS LAGMQAPLRD GALDLIGLGR ILAIEPDAPA RLLRGEETRH RVKPLSTGVK YLDSLGSLEV TWYTRQLHRI GKGRNPVPNE NALKSFLLDL SSKGCAIFKA RRLRASSSEA SAAHGSARSI AGGQRHDAH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg attcgaatcc cgccgcggcc gcgttgctcg cgcagccgct cacgctgccg aacggcaccg tgctgaagaa ccgcatcgcg aaatcggcgc tgagcgaagc gctgtgcggt cacgacggcc gcgtgacgcc gcagctgatc gcgctgtacc ggcgctggtc gaattccggc gcggggctgc tcgtcaccgg caacgtgatg atcgacggca aggcgctcgg cgagcccggc aacgtcgtga tcgaggacga gcgtgacctg ccgcgtctgc gcgaatgggc cgaggccgcg aaatcgcacg gcagcgagat ctggatgcag atcaatcacc cgggcaagca ggcgatgcgc ggcctgaacg cggagaccgt cgcgccgtcg gcgatcggct tcgggccgaa gctgtcgccg tatttcgcgg tgccgcgcgc gctgacgggc gacgagatcg gcgagctgat ccggcgctac ggaacgacgg ccgccgtcgc gcagcaggcg ggcttcaccg gcgtgcaact gcacggcgcg cacggctatc tgatcaacca gttcctgtcg ccgcagcaca atcagcgcac cgacgaatgg ggcggcagcg cggagaaccg ccggcgcttc gtgctcgagg tgtacgcgca agtgcggcgc cgggtgggcc ctgattttcc gatcggcatc aagctcaatt cggccgactt ccagcgcggc ggcttcaccg aggaagaatc gctcgacgtg atccgcgcgc tcgccgaagc gggcatcgat ctgatcgaga tttccggcgg cacctacgaa acgcccgtga tgcagctcgg cgaccgcaag gcgtcgactg tcgcgcgcga ggcgtacttc ctcgcattcg ccgagaaggt gcgcgccgaa gtgaaggtgc cgctgatggt cacgggcggc tttcgcagcc tcgccggcat gcaagcgccg ctgcgcgacg gcgcgctcga cctgatcggc ctcgggcgca ttctcgcgat cgagcccgac gcgcccgccc gcctgctgcg cggcgaggaa acgcggcatc gcgtgaagcc gctcagcacg ggcgtcaaat acctcgacag tctcggttcg ctcgaggtca cgtggtatac gcgccagctc catcgaatcg gcaagggccg caatccggtc cccaacgaga acgcgctgaa gtcgttcctg ctcgatttgt cgtccaaggg ctgcgcgatc ttcaaggcgc ggcgcctgcg tgcttcgtca tccgaagcgt cggccgcaca tggctcggcg cgttcgatcg ccggcgggca gcggcacgac gcgcattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t