ButhA.00093.b

Oxidoreductase, FMN-binding

CENTER ID: ButhA.00093.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00093.b.B1.GE36098 Full length( ButhA.00093.b ) 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2375
RefSeq: YP_440340.1
UniProt: Q2T3A9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQDPLFEPL RLGVLMLPNR IVMPPMTRSR ASRPGDEAND LMAAYYAQRA SAGLIVSEGT YIAPLGKGYA WTPGIHTPAQ VAGWRKVTRA VHAAGGRMFA QLWHVGRLSH VSLLGGRQPV SSSPIQAKGV NVFIAGDDGS TPGFVQASEP RALGVDEIRE IVGQYRAAAR HAIDAGFDGV ELHGANGYLV NQFIDSQANT RTDEYGGSLG NRLRFLREVA QALIEGAGDA SRVGVRLAPL TTLNGCVDDD PQTTYLAAAK LLGELGVGYL HIAEADWDDA PLMPVEFKRK LREAFPGVLI YAGKYTAQRA REAIAAGWAD LIAFGRPFVA NPDLPERLRA GAPLAPHDRS TLFGGGAHGL TDYPTLAQDA A
NT Sequence
ATGACTCAGG ACCCTCTGTT CGAACCGCTG CGGCTCGGCG TGCTGATGCT GCCGAACCGC ATCGTGATGC CGCCGATGAC GCGCTCGCGC GCGAGCCGGC CCGGCGACGA AGCCAACGAC CTGATGGCCG CGTATTACGC GCAGCGCGCG AGCGCGGGCC TCATCGTCAG CGAAGGCACG TACATCGCGC CGCTCGGCAA GGGCTATGCG TGGACCCCCG GCATTCACAC GCCCGCGCAG GTCGCCGGCT GGCGCAAGGT CACGCGCGCC GTGCACGCCG CCGGCGGGCG GATGTTCGCG CAGCTCTGGC ACGTCGGCCG GCTGAGCCAC GTGAGCCTGC TGGGCGGCCG GCAGCCGGTG TCGTCGTCGC CGATCCAGGC GAAGGGCGTG AACGTGTTCA TCGCGGGCGA CGACGGCAGC ACGCCGGGCT TCGTGCAGGC GTCCGAGCCG CGCGCGCTCG GCGTCGACGA GATCCGCGAG ATCGTCGGCC AGTATCGCGC CGCCGCGCGC CATGCGATCG ACGCGGGCTT CGACGGCGTC GAGCTGCACG GCGCGAACGG CTACCTCGTG AACCAGTTCA TCGATTCGCA GGCGAACACG CGCACCGACG AATACGGCGG CTCGCTCGGC AACCGGCTGC GCTTCCTGCG CGAAGTCGCG CAGGCGTTGA TCGAAGGCGC GGGCGATGCG TCGCGGGTTG GCGTTCGCCT CGCGCCGCTG ACGACGCTGA ACGGCTGCGT CGACGACGAT CCGCAAACGA CGTACCTCGC GGCCGCGAAG CTGCTCGGCG AGCTCGGCGT CGGCTACCTG CACATCGCGG AAGCCGACTG GGACGATGCG CCGCTGATGC CGGTCGAGTT CAAGCGGAAG CTGCGCGAGG CGTTCCCGGG CGTGCTGATC TATGCGGGCA AGTACACGGC GCAACGCGCG CGCGAGGCGA TCGCGGCCGG CTGGGCGGAT CTGATCGCGT TCGGCCGCCC GTTCGTCGCG AACCCGGACT TGCCCGAACG CCTGCGCGCC GGCGCGCCGC TCGCGCCGCA CGATCGCAGC ACGCTGTTCG GCGGCGGCGC GCACGGCCTT ACCGATTATC CGACCCTCGC GCAAGACGCG GCG
Details for ButhA.00093.b.B1.GE36098
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 89
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHHM TQDPLFEPLR LGVLMLPNRI VMPPMTRSRA SRPGDEANDL MAAYYAQRAS AGLIVSEGTY IAPLGKGYAW TPGIHTPAQV AGWRKVTRAV HAAGGRMFAQ LWHVGRLSHV SLLGGRQPVS SSPIQAKGVN VFIAGDDGXT PGFVQASEPR ALXVDEIXEI VGQYRAAARH AIDAGFXGVE LHGANGYXXN XFINSQANTR XXEYGGSLGN XLXFLRXXXX ALIEXAXDAX RVGXRLXXXT X
Validated NT Sequence
tttntttact ttaagaagga ganataccat ggctcaccac caccaccacc accatatgac tcaggaccct ctgttcgaac cgctgcggct cggcgtgctg atgctgccga accgcatcgt gatgccgccg atgacgcgct cgcgcgcgag ccggcccggc gacgaagcca acgacctgat ggccgcgtat tacgcgcagc gcgcgagcgc gggcctcatc gtcagcgaag gcacgtacat cgcgccgctc ggcaagggct atgcgtggac ccccggcatt cacacgcccg cgcaggtcgc cggctggcgc aaggtcacgc gcgccgtgca cgccgccggc gggcggatgt tcgcgcagct ctggcacgtc ggccggctga gccacgtgag cctgctgggc ggccggcagc cggtgtcgtc gtcnccgatc caggcgaagg gcgtgaacgt gttcatcgcg ggcgacgacg gcngcacgcc gggcttcgtg caggcgtccg agccgcgcgc gctcngcgtc gacgagatcc ncgagatcgt cggccagtat cgcgccgccg cgcgccatgc natcgacgcg ggcttcnacg gcgtcgagct gcacggcgcg aacggctacn tcntgaacca nttcatcaat tcncaggcga acacgcgcnc nnacgaatac ggcggctcgc tcggcaacng gctgcncttc ctgcgcnaan nnnngcangc gttgatcgaa ngcgcgngcg atgcnncncg ngttggcntt cgnctcgnnn nnntgacnan ntgaacggct gcntcnnnna cnatnnnnnn cgnnntncnn nngnnnnnnn nctgctnngn nnnnncgcgt cngctancnn nnnnnnnnnn nncngactgg ccnnnnnnnn gcnnnncnnt cnnnngcaan nnnnncnnnn nnnnccntgn nnntctnnnc nnnnannnnn nnnnnnnnnn nnnnncnnga nnngatnnnn nnnnnnnnnn gnnnnnnnat cnnnnnnnnn cccnnntnnt nnnnnannnn nnnnnangnn nnnnnnnnnn nnnnnnnnnn nnnngnnnnn nnnnnnnnna nnnnnnnnnn nnnnnnnntn nnnnnnnnnn nnnana
Expected Protein Sequence
MAHHHHHHMT QDPLFEPLRL GVLMLPNRIV MPPMTRSRAS RPGDEANDLM AAYYAQRASA GLIVSEGTYI APLGKGYAWT PGIHTPAQVA GWRKVTRAVH AAGGRMFAQL WHVGRLSHVS LLGGRQPVSS SPIQAKGVNV FIAGDDGSTP GFVQASEPRA LGVDEIREIV GQYRAAARHA IDAGFDGVEL HGANGYLVNQ FIDSQANTRT DEYGGSLGNR LRFLREVAQA LIEGAGDASR VGVRLAPLTT LNGCVDDDPQ TTYLAAAKLL GELGVGYLHI AEADWDDAPL MPVEFKRKLR EAFPGVLIYA GKYTAQRARE AIAAGWADLI AFGRPFVANP DLPERLRAGA PLAPHDRSTL FGGGAHGLTD YPTLAQDAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactc aggaccctct gttcgaaccg ctgcggctcg gcgtgctgat gctgccgaac cgcatcgtga tgccgccgat gacgcgctcg cgcgcgagcc ggcccggcga cgaagccaac gacctgatgg ccgcgtatta cgcgcagcgc gcgagcgcgg gcctcatcgt cagcgaaggc acgtacatcg cgccgctcgg caagggctat gcgtggaccc ccggcattca cacgcccgcg caggtcgccg gctggcgcaa ggtcacgcgc gccgtgcacg ccgccggcgg gcggatgttc gcgcagctct ggcacgtcgg ccggctgagc cacgtgagcc tgctgggcgg ccggcagccg gtgtcgtcgt cgccgatcca ggcgaagggc gtgaacgtgt tcatcgcggg cgacgacggc agcacgccgg gcttcgtgca ggcgtccgag ccgcgcgcgc tcggcgtcga cgagatccgc gagatcgtcg gccagtatcg cgccgccgcg cgccatgcga tcgacgcggg cttcgacggc gtcgagctgc acggcgcgaa cggctacctc gtgaaccagt tcatcgattc gcaggcgaac acgcgcaccg acgaatacgg cggctcgctc ggcaaccggc tgcgcttcct gcgcgaagtc gcgcaggcgt tgatcgaagg cgcgggcgat gcgtcgcggg ttggcgttcg cctcgcgccg ctgacgacgc tgaacggctg cgtcgacgac gatccgcaaa cgacgtacct cgcggccgcg aagctgctcg gcgagctcgg cgtcggctac ctgcacatcg cggaagccga ctgggacgat gcgccgctga tgccggtcga gttcaagcgg aagctgcgcg aggcgttccc gggcgtgctg atctatgcgg gcaagtacac ggcgcaacgc gcgcgcgagg cgatcgcggc cggctgggcg gatctgatcg cgttcggccg cccgttcgtc gcgaacccgg acttgcccga acgcctgcgc gccggcgcgc cgctcgcgcc gcacgatcgc agcacgctgt tcggcggcgg cgcgcacggc cttaccgatt atccgaccct cgcgcaagac gcggcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t