SSGCID: Target Page

ButhA.00093.a

Oxidoreductase, FAD/FMN-binding

CENTER ID:	ButhA.00093.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00093.a.B1.GE36097	Full length( ButhA.00093.a )		1	370

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00093.a.B1.PW36743	Full length( ButhA.00093.a )		1	370

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.6232
RefSeq:	YP_443834.1
UniProt:	Q2STB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSALFSPFTL RGVTLPNRIV ISPMCQYSAE NGEARAWHMI HLGHLALSGA GLLCIEATAV EPDGRISPAD LGLWNDATEA ALEPVLAAIR KHSPIRVAMQ LSHAGRKASS DVPWNGGQLI PADQGGWQPH APSAVPHREH EPSPLALDSA GLERVRCAFV ESAKRAARLG IDAIEVHAAH GYLLHQFLSP LANHRTDAYG GSLENRMRFP LEVFDAVRAA FPDDRPVGVR VSATDWVTGG WELDDTIAFA RELKRRGCDW IDVSSGGVSP LQKIPLSPGY QVPFAQAIKR AVDLPTIAVG LISDPEHANR VIEAGDADFV AMARAMLYDP RWPWHAAAQL GATVSAPPQY WRSQPREYKA LFGDVSFGQR

NT Sequence

ATGAGTGCGT TGTTTTCTCC GTTCACGCTG CGCGGCGTGA CCCTTCCCAA TCGCATCGTG ATTTCGCCGA TGTGCCAGTA TTCGGCCGAA AACGGCGAGG CGCGCGCATG GCACATGATT CATCTAGGCC ACCTCGCGTT GTCCGGGGCG GGGCTGCTTT GCATCGAAGC GACGGCTGTC GAGCCTGACG GCCGCATTTC ACCCGCGGAT CTCGGCTTGT GGAACGACGC GACCGAAGCC GCGCTCGAAC CCGTGCTCGC GGCGATCCGC AAGCATTCGC CGATTCGCGT CGCGATGCAG CTGTCGCACG CGGGCCGCAA GGCGTCGAGC GACGTGCCAT GGAACGGCGG GCAACTGATT CCCGCCGACC AGGGCGGCTG GCAGCCGCAT GCGCCTTCCG CCGTCCCGCA CCGGGAGCAC GAGCCTTCAC CGCTCGCGCT CGACTCAGCG GGGCTCGAGC GAGTTCGTTG CGCGTTCGTC GAATCCGCGA AGCGCGCGGC GCGTCTTGGC ATCGACGCGA TCGAAGTCCA TGCGGCGCAC GGTTATCTTC TGCATCAGTT CCTGTCGCCG CTCGCGAACC ATCGCACCGA TGCGTACGGC GGCTCGCTCG AAAACCGGAT GCGCTTTCCG CTCGAGGTGT TCGACGCGGT GCGTGCGGCG TTTCCGGACG ATCGTCCGGT CGGCGTGCGC GTGTCGGCGA CCGACTGGGT GACGGGCGGC TGGGAGCTTG ACGATACGAT CGCGTTCGCG CGCGAATTGA AGCGGCGCGG CTGCGACTGG ATCGACGTGT CGTCCGGCGG CGTGTCGCCG CTTCAGAAGA TTCCGTTGTC GCCCGGCTAT CAGGTGCCGT TCGCTCAGGC GATCAAGCGC GCGGTCGACC TGCCGACGAT TGCCGTCGGC CTCATTTCTG ATCCCGAGCA CGCGAATCGC GTGATCGAAG CGGGCGACGC GGATTTCGTC GCGATGGCGC GCGCGATGCT CTACGATCCG CGCTGGCCGT GGCATGCGGC CGCGCAGCTC GGTGCGACGG TCAGCGCGCC GCCGCAGTAC TGGCGTTCGC AGCCGCGCGA GTACAAGGCG TTGTTCGGCG ACGTATCGTT CGGCCAGCGT

Details for ButhA.00093.a.B1.GE36097

HARVESTED ON:	9/26/2012
SEQUENCED ON:	9/27/2012
EXPECTED MW:	41kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	89

Validated AA Sequence

MCQYSAENGE ARAWHMIHLG HLALSGAGLL CIEATAVEPD GRISPADLGL WNDATEAALE PVLAAIRKHS PIRVAMQLSH AGRKASSDVP WNGGQLIPAD QGGWQPHAPS AVPHREHEPS PLALDSAGLE RVRCAFVESA KRAARLGIDA IEVHAAHGYL LHQFLSPLAN HRTDAYGGSL ENRMRFPLEV FDAVRAAFPD DRPVGVRVSA TDWVTGGWEL DDTIAFAREL KRRGCDWIDV SSGGVSPLQK IPLSPGYQVP FAQAIKRAVD LPTIAVGLIS DPEHANRVIE AGDADFVAMA RAMLYDPRWP WHAAAQLGAT VSAPPQYWRS QPREYKAL

Validated NT Sequence

aacaacgcct tgtactcgcg cggctgcgaa cgccagtact gcggcggcgc gctgaccgtc gcaccgagct gcgcggccgc atgccacggc cagcgcggat cgtagagcat cgcgcgcgcc atcgcgacga aatccgcgtc gcccgcttcg atcacgcgat tcgcgtgctc gggatcagaa atgaggccga cggcaatcgt cggcaggtcg accgcgcgct tgatcgcctg agcgaacggc acctgatagc cgggcgacaa cggaatcttc tgaagcggcg acacgccgcc ggacgacacg tcgatccagt cgcagccgcg ccgcttcaat tcgcgcgcga acgcgatcgt atcgtcaagc tcccagccgc ccgtcaccca gtcggtcgcc gacacgcgca cgccgaccgg acgatcgtcc ggaaacgccg cacgcaccgc gtcgaacacc tcgagcggaa agcgcatccg gttttcgagc gagccgccgt acgcatcggt gcgatggttc gcgagcggcg acaggaactg atgcagaaga taaccgtgcg ccgcatggac ttcgatcgcg tcgatgccaa gacgcgccgc gcgcttcgcg gattcgacga acgcgcaacg aactcgctcg agccccgctg agtcgagcgc gagcggtgaa ggctcgtgct cccggtgcgg gacggcggaa ggcgcatgcg gctgccagcc gccctggtcg gcgggaatca gttgcccgcc gttccatggc acgtcgctcg acgccttgcg gcccgcgtgc gacagctgca tcgcgacgcg aatcggcgaa tgcttgcgga tcgccgcgag cacgggttcg agcgcggctt cggtcgcgtc gttccacaag ccgagatccg cgggtgaaat gcggccgtca ggctcgacag ccgtcgcttc gatgcaaagc agccccgccc cggacaacgc gaggtggcct agatgaatca tgtgccatgc gcgcgcctcg ccgttttcgg ccgaatactg gcacatcggc gaaatcacga tgcgattggg aagggtcacg ccgcgcagcg tgaacggaga aaaca

Expected Protein Sequence

MAHHHHHHMS ALFSPFTLRG VTLPNRIVIS PMCQYSAENG EARAWHMIHL GHLALSGAGL LCIEATAVEP DGRISPADLG LWNDATEAAL EPVLAAIRKH SPIRVAMQLS HAGRKASSDV PWNGGQLIPA DQGGWQPHAP SAVPHREHEP SPLALDSAGL ERVRCAFVES AKRAARLGID AIEVHAAHGY LLHQFLSPLA NHRTDAYGGS LENRMRFPLE VFDAVRAAFP DDRPVGVRVS ATDWVTGGWE LDDTIAFARE LKRRGCDWID VSSGGVSPLQ KIPLSPGYQV PFAQAIKRAV DLPTIAVGLI SDPEHANRVI EAGDADFVAM ARAMLYDPRW PWHAAAQLGA TVSAPPQYWR SQPREYKALF GDVSFGQR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg cgttgttttc tccgttcacg ctgcgcggcg tgacccttcc caatcgcatc gtgatttcgc cgatgtgcca gtattcggcc gaaaacggcg aggcgcgcgc atggcacatg attcatctag gccacctcgc gttgtccggg gcggggctgc tttgcatcga agcgacggct gtcgagcctg acggccgcat ttcacccgcg gatctcggct tgtggaacga cgcgaccgaa gccgcgctcg aacccgtgct cgcggcgatc cgcaagcatt cgccgattcg cgtcgcgatg cagctgtcgc acgcgggccg caaggcgtcg agcgacgtgc catggaacgg cgggcaactg attcccgccg accagggcgg ctggcagccg catgcgcctt ccgccgtccc gcaccgggag cacgagcctt caccgctcgc gctcgactca gcggggctcg agcgagttcg ttgcgcgttc gtcgaatccg cgaagcgcgc ggcgcgtctt ggcatcgacg cgatcgaagt ccatgcggcg cacggttatc ttctgcatca gttcctgtcg ccgctcgcga accatcgcac cgatgcgtac ggcggctcgc tcgaaaaccg gatgcgcttt ccgctcgagg tgttcgacgc ggtgcgtgcg gcgtttccgg acgatcgtcc ggtcggcgtg cgcgtgtcgg cgaccgactg ggtgacgggc ggctgggagc ttgacgatac gatcgcgttc gcgcgcgaat tgaagcggcg cggctgcgac tggatcgacg tgtcgtccgg cggcgtgtcg ccgcttcaga agattccgtt gtcgcccggc tatcaggtgc cgttcgctca ggcgatcaag cgcgcggtcg acctgccgac gattgccgtc ggcctcattt ctgatcccga gcacgcgaat cgcgtgatcg aagcgggcga cgcggatttc gtcgcgatgg cgcgcgcgat gctctacgat ccgcgctggc cgtggcatgc ggccgcgcag ctcggtgcga cggtcagcgc gccgccgcag tactggcgtt cgcagccgcg cgagtacaag gcgttgttcg gcgacgtatc gttcggccag cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for ButhA.00093.a.B1.PW36743

PURIFICATION DATe:	11/6/2012
CONCENTRATION:	39.4mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	23
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	89

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTG CGTTGTTTTC TCCGTTCACG CTGCGCGGCG TGACCCTTCC CAATCGCATC GTGATTTCGC CGATGTGCCA GTATTCGGCC GAAAACGGCG AGGCGCGCGC ATGGCACATG ATTCATCTAG GCCACCTCGC GTTGTCCGGG GCGGGGCTGC TTTGCATCGA AGCGACGGCT GTCGAGCCTG ACGGCCGCAT TTCACCCGCG GATCTCGGCT TGTGGAACGA CGCGACCGAA GCCGCGCTCG AACCCGTGCT CGCGGCGATC CGCAAGCATT CGCCGATTCG CGTCGCGATG CAGCTGTCGC ACGCGGGCCG CAAGGCGTCG AGCGACGTGC CATGGAACGG CGGGCAACTG ATTCCCGCCG ACCAGGGCGG CTGGCAGCCG CATGCGCCTT CCGCCGTCCC GCACCGGGAG CACGAGCCTT CACCGCTCGC GCTCGACTCA GCGGGGCTCG AGCGAGTTCG TTGCGCGTTC GTCGAATCCG CGAAGCGCGC GGCGCGTCTT GGCATCGACG CGATCGAAGT CCATGCGGCG CACGGTTATC TTCTGCATCA GTTCCTGTCG CCGCTCGCGA ACCATCGCAC CGATGCGTAC GGCGGCTCGC TCGAAAACCG GATGCGCTTT CCGCTCGAGG TGTTCGACGC GGTGCGTGCG GCGTTTCCGG ACGATCGTCC GGTCGGCGTG CGCGTGTCGG CGACCGACTG GGTGACGGGC GGCTGGGAGC TTGACGATAC GATCGCGTTC GCGCGCGAAT TGAAGCGGCG CGGCTGCGAC TGGATCGACG TGTCGTCCGG CGGCGTGTCG CCGCTTCAGA AGATTCCGTT GTCGCCCGGC TATCAGGTGC CGTTCGCTCA GGCGATCAAG CGCGCGGTCG ACCTGCCGAC GATTGCCGTC GGCCTCATTT CTGATCCCGA GCACGCGAAT CGCGTGATCG AAGCGGGCGA CGCGGATTTC GTCGCGATGG CGCGCGCGAT GCTCTACGAT CCGCGCTGGC CGTGGCATGC GGCCGCGCAG CTCGGTGCGA CGGTCAGCGC GCCGCCGCAG TACTGGCGTT CGCAGCCGCG CGAGTACAAG GCGTTGTTCG GCGACGTATC GTTCGGCCAG CGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT