ButhA.00092.b

Tartrate dehydrogenase

CENTER ID: ButhA.00092.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00092.b.B1.GE36096 Full length( ButhA.00092.b ) 1 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00092.b.B1.PS01689 Full length( ButhA.00092.b ) 1 361

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.14
RefSeq: YP_438215.1
UniProt: Q2T9D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDKVYRIAV IPGDGIGVEV MPEGLRALDA VSRRFGLRFE YEPIEWASCG YYAKHGQMMP DDWKAQLSGM DALLFGAVGW PETVPDHVSL WGSLIKFRRE FDQYVNLRPA RLFDGVPCPL AGRKAGDIDF MIVRENTEGE YSAVGGTMFE GTEREFVVQQ AVFTRHGTER VMKFAFELAQ RRAKRLTVAT KSNGIAISMP WWDARAAEMA ARYPDVAWDK QHIDILCARF VMQPDRFDVV VASNLFGDIL SDLGPACTGT IGIAPSANLN PERNFPSLFE PVHGSAPDIA GKQVANPIAM IWSAAMMIDF LGEGAGPARE AHDAIVAAIE HVLKHGPRTR DMGGQASTSE VGDAIVARLL G
NT Sequence
ATGAGCGACA AGGTGTACAG GATCGCGGTC ATCCCCGGCG ACGGAATCGG CGTGGAAGTG ATGCCCGAAG GGCTGCGCGC GCTCGATGCG GTGAGCCGCC GCTTCGGCCT GCGCTTCGAG TACGAGCCGA TCGAATGGGC AAGCTGCGGC TACTACGCGA AGCACGGCCA GATGATGCCG GACGACTGGA AGGCGCAACT TTCCGGCATG GACGCGCTGT TGTTCGGCGC GGTGGGCTGG CCCGAGACGG TGCCCGATCA CGTGTCGCTG TGGGGCTCGC TGATCAAGTT CCGCCGAGAG TTCGATCAAT ACGTGAACCT GCGCCCCGCG CGACTGTTCG ACGGCGTACC GTGCCCGCTC GCCGGCCGCA AGGCAGGCGA CATCGATTTC ATGATCGTGC GCGAGAACAC CGAGGGCGAA TACTCGGCCG TCGGCGGCAC GATGTTCGAG GGCACCGAGC GCGAATTCGT CGTCCAGCAG GCCGTGTTCA CGCGGCACGG CACCGAGCGC GTGATGAAAT TCGCGTTCGA GCTCGCGCAG CGCCGCGCGA AACGGCTCAC CGTCGCGACG AAGAGCAACG GCATCGCGAT CAGCATGCCG TGGTGGGACG CACGCGCCGC CGAGATGGCC GCGCGTTATC CGGACGTCGC GTGGGACAAG CAGCACATCG ACATCCTGTG CGCGCGCTTC GTGATGCAGC CGGACCGCTT CGACGTCGTC GTCGCGTCGA ACCTGTTCGG CGACATCCTG TCCGATCTCG GCCCCGCATG CACCGGCACG ATCGGCATCG CGCCGTCGGC GAACCTGAAC CCGGAGCGCA ACTTTCCGTC GCTGTTCGAG CCGGTGCACG GCTCCGCGCC GGACATCGCG GGCAAACAGG TCGCGAATCC GATCGCGATG ATCTGGTCGG CCGCGATGAT GATCGATTTC CTCGGCGAAG GCGCGGGCCC CGCCCGTGAA GCGCACGATG CGATCGTCGC GGCGATCGAG CACGTGCTGA AGCACGGCCC GCGCACGCGC GACATGGGCG GCCAGGCGTC GACAAGCGAA GTGGGCGACG CGATCGTCGC GCGCCTGCTC GGC
Details for ButhA.00092.b.B1.GE36096
HARVESTED ON: 9/26/2012
SEQUENCED ON: 9/25/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Validated AA Sequence
MPEGLRALDA VSRRFGLRFE YEPIEWASCG YYAKHGQMMP DDWKAQLSGM DALLFGAVGW PETVPDHVSL WGSLIKFRRE FDQYVNLRPA RLFDGVPCPL AGRKAGDIDF MIVRENTEGE YSAVGGTMFE GTEREFVVQQ AVFTRHGTER VMKFAFELAQ RRAKRLTVAT KSNGIAISMP WWDARAAEMA ARYPDVAWDK QHIDILCARF VMQPDRFDVV VASNLFGDIL SDLGPACTGT IGIAPSANLN PERNFPSLFE PVHGSAPDIA GKQVANPIAM IWSAAMMIDF LGEGAGPARE AHDAIVAAIE HVLKHGPRTR DMGGQASTSE VGDAIVAXL
Validated NT Sequence
agcaggngcg cgacgatcgc gtcgcccact tcgcttgtcg acgcctggcc gcccatgtcg cgcgtgcgcg ggccgtgctt cagcacgtgc tcgatcgccg cgacgatcgc atcgtgcgct tcacgggcgg ggcccgcgcc ttcgccgagg aaatcgatca tcatcgcggc cgaccagatc atcgcgatcg gattcgcgac ctgtttgccc gcgatgtccg gcgcggagcc gtgcaccggc tcgaacagcg acggaaagtt gcgctccggg ttcaggttcg ccgacggcgc gatgccgatc gtgccggtgc atgcggggcc gagatcggac aggatgtcgc cgaacaggtt cgacgcgacg acgacgtcga agcggtccgg ctgcatcacg aagcgcgcgc acaggatgtc gatgtgctgc ttgtcccacg cgacgtccgg ataacgcgcg gccatctcgg cggcgcgtgc gtcccaccac ggcatgctga tcgcgatgcc gttgctcttc gtcgcgacgg tgagccgttt cgcgcggcgc tgcgcgagct cgaacgcgaa tttcatcacg cgctcggtgc cgtgccgcgt gaacacggcc tgctggacga cgaattcgcg ctcggtgccc tcgaacatcg tgccgccgac ggccgagtat tcgccctcgg tgttctcgcg cacgatcatg aaatcgatgt cgcctgcctt gcggccggcg agcgggcacg gtacgccgtc gaacagtcgc gcggggcgca ggttcacgta ttgatcgaac tctcggcgga acttgatcag cgagccccac agcgacacgt gatcgggcac cgtctcgggc cagcccaccg cgccgaacaa cagcgcgtcc atgccggaaa gttgcgcctt ccagtcgtcc ggcatcatct ggccgtgctt cgcgtagtag ccgcagcttg cccattcgat cggctcgtac tcgaagcgca ggccgaagcg gcggctcacc gcatcgagcg cgcgcagccc ttcgggcatc acttccacgc cgattccgtc gccggggatg accgcgatcc tgtacacctt gt
Expected Protein Sequence
MAHHHHHHMS DKVYRIAVIP GDGIGVEVMP EGLRALDAVS RRFGLRFEYE PIEWASCGYY AKHGQMMPDD WKAQLSGMDA LLFGAVGWPE TVPDHVSLWG SLIKFRREFD QYVNLRPARL FDGVPCPLAG RKAGDIDFMI VRENTEGEYS AVGGTMFEGT EREFVVQQAV FTRHGTERVM KFAFELAQRR AKRLTVATKS NGIAISMPWW DARAAEMAAR YPDVAWDKQH IDILCARFVM QPDRFDVVVA SNLFGDILSD LGPACTGTIG IAPSANLNPE RNFPSLFEPV HGSAPDIAGK QVANPIAMIW SAAMMIDFLG EGAGPAREAH DAIVAAIEHV LKHGPRTRDM GGQASTSEVG DAIVARLLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acaaggtgta caggatcgcg gtcatccccg gcgacggaat cggcgtggaa gtgatgcccg aagggctgcg cgcgctcgat gcggtgagcc gccgcttcgg cctgcgcttc gagtacgagc cgatcgaatg ggcaagctgc ggctactacg cgaagcacgg ccagatgatg ccggacgact ggaaggcgca actttccggc atggacgcgc tgttgttcgg cgcggtgggc tggcccgaga cggtgcccga tcacgtgtcg ctgtggggct cgctgatcaa gttccgccga gagttcgatc aatacgtgaa cctgcgcccc gcgcgactgt tcgacggcgt accgtgcccg ctcgccggcc gcaaggcagg cgacatcgat ttcatgatcg tgcgcgagaa caccgagggc gaatactcgg ccgtcggcgg cacgatgttc gagggcaccg agcgcgaatt cgtcgtccag caggccgtgt tcacgcggca cggcaccgag cgcgtgatga aattcgcgtt cgagctcgcg cagcgccgcg cgaaacggct caccgtcgcg acgaagagca acggcatcgc gatcagcatg ccgtggtggg acgcacgcgc cgccgagatg gccgcgcgtt atccggacgt cgcgtgggac aagcagcaca tcgacatcct gtgcgcgcgc ttcgtgatgc agccggaccg cttcgacgtc gtcgtcgcgt cgaacctgtt cggcgacatc ctgtccgatc tcggccccgc atgcaccggc acgatcggca tcgcgccgtc ggcgaacctg aacccggagc gcaactttcc gtcgctgttc gagccggtgc acggctccgc gccggacatc gcgggcaaac aggtcgcgaa tccgatcgcg atgatctggt cggccgcgat gatgatcgat ttcctcggcg aaggcgcggg ccccgcccgt gaagcgcacg atgcgatcgt cgcggcgatc gagcacgtgc tgaagcacgg cccgcgcacg cgcgacatgg gcggccaggc gtcgacaagc gaagtgggcg acgcgatcgt cgcgcgcctg ctcggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for ButhA.00092.b.B1.PS01689
PURIFICATION DATe: 11/27/2012
CONCENTRATION: 74.6mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MPEGLRALDA VSRRFGLRFE YEPIEWASCG YYAKHGQMMP DDWKAQLSGM DALLFGAVGW PETVPDHVSL WGSLIKFRRE FDQYVNLRPA RLFDGVPCPL AGRKAGDIDF MIVRENTEGE YSAVGGTMFE GTEREFVVQQ AVFTRHGTER VMKFAFELAQ RRAKRLTVAT KSNGIAISMP WWDARAAEMA ARYPDVAWDK QHIDILCARF VMQPDRFDVV VASNLFGDIL SDLGPACTGT IGIAPSANLN PERNFPSLFE PVHGSAPDIA GKQVANPIAM IWSAAMMIDF LGEGAGPARE AHDAIVAAIE HVLKHGPRTR DMGGQASTSE VGDAIVAXL
Validated NT Sequence
agcaggngcg cgacgatcgc gtcgcccact tcgcttgtcg acgcctggcc gcccatgtcg cgcgtgcgcg ggccgtgctt cagcacgtgc tcgatcgccg cgacgatcgc atcgtgcgct tcacgggcgg ggcccgcgcc ttcgccgagg aaatcgatca tcatcgcggc cgaccagatc atcgcgatcg gattcgcgac ctgtttgccc gcgatgtccg gcgcggagcc gtgcaccggc tcgaacagcg acggaaagtt gcgctccggg ttcaggttcg ccgacggcgc gatgccgatc gtgccggtgc atgcggggcc gagatcggac aggatgtcgc cgaacaggtt cgacgcgacg acgacgtcga agcggtccgg ctgcatcacg aagcgcgcgc acaggatgtc gatgtgctgc ttgtcccacg cgacgtccgg ataacgcgcg gccatctcgg cggcgcgtgc gtcccaccac ggcatgctga tcgcgatgcc gttgctcttc gtcgcgacgg tgagccgttt cgcgcggcgc tgcgcgagct cgaacgcgaa tttcatcacg cgctcggtgc cgtgccgcgt gaacacggcc tgctggacga cgaattcgcg ctcggtgccc tcgaacatcg tgccgccgac ggccgagtat tcgccctcgg tgttctcgcg cacgatcatg aaatcgatgt cgcctgcctt gcggccggcg agcgggcacg gtacgccgtc gaacagtcgc gcggggcgca ggttcacgta ttgatcgaac tctcggcgga acttgatcag cgagccccac agcgacacgt gatcgggcac cgtctcgggc cagcccaccg cgccgaacaa cagcgcgtcc atgccggaaa gttgcgcctt ccagtcgtcc ggcatcatct ggccgtgctt cgcgtagtag ccgcagcttg cccattcgat cggctcgtac tcgaagcgca ggccgaagcg gcggctcacc gcatcgagcg cgcgcagccc ttcgggcatc acttccacgc cgattccgtc gccggggatg accgcgatcc tgtacacctt gt
Expressed Protein Sequence
MAHHHHHHMS DKVYRIAVIP GDGIGVEVMP EGLRALDAVS RRFGLRFEYE PIEWASCGYY AKHGQMMPDD WKAQLSGMDA LLFGAVGWPE TVPDHVSLWG SLIKFRREFD QYVNLRPARL FDGVPCPLAG RKAGDIDFMI VRENTEGEYS AVGGTMFEGT EREFVVQQAV FTRHGTERVM KFAFELAQRR AKRLTVATKS NGIAISMPWW DARAAEMAAR YPDVAWDKQH IDILCARFVM QPDRFDVVVA SNLFGDILSD LGPACTGTIG IAPSANLNPE RNFPSLFEPV HGSAPDIAGK QVANPIAMIW SAAMMIDFLG EGAGPAREAH DAIVAAIEHV LKHGPRTRDM GGQASTSEVG DAIVARLLG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACAAGGTGTA CAGGATCGCG GTCATCCCCG GCGACGGAAT CGGCGTGGAA GTGATGCCCG AAGGGCTGCG CGCGCTCGAT GCGGTGAGCC GCCGCTTCGG CCTGCGCTTC GAGTACGAGC CGATCGAATG GGCAAGCTGC GGCTACTACG CGAAGCACGG CCAGATGATG CCGGACGACT GGAAGGCGCA ACTTTCCGGC ATGGACGCGC TGTTGTTCGG CGCGGTGGGC TGGCCCGAGA CGGTGCCCGA TCACGTGTCG CTGTGGGGCT CGCTGATCAA GTTCCGCCGA GAGTTCGATC AATACGTGAA CCTGCGCCCC GCGCGACTGT TCGACGGCGT ACCGTGCCCG CTCGCCGGCC GCAAGGCAGG CGACATCGAT TTCATGATCG TGCGCGAGAA CACCGAGGGC GAATACTCGG CCGTCGGCGG CACGATGTTC GAGGGCACCG AGCGCGAATT CGTCGTCCAG CAGGCCGTGT TCACGCGGCA CGGCACCGAG CGCGTGATGA AATTCGCGTT CGAGCTCGCG CAGCGCCGCG CGAAACGGCT CACCGTCGCG ACGAAGAGCA ACGGCATCGC GATCAGCATG CCGTGGTGGG ACGCACGCGC CGCCGAGATG GCCGCGCGTT ATCCGGACGT CGCGTGGGAC AAGCAGCACA TCGACATCCT GTGCGCGCGC TTCGTGATGC AGCCGGACCG CTTCGACGTC GTCGTCGCGT CGAACCTGTT CGGCGACATC CTGTCCGATC TCGGCCCCGC ATGCACCGGC ACGATCGGCA TCGCGCCGTC GGCGAACCTG AACCCGGAGC GCAACTTTCC GTCGCTGTTC GAGCCGGTGC ACGGCTCCGC GCCGGACATC GCGGGCAAAC AGGTCGCGAA TCCGATCGCG ATGATCTGGT CGGCCGCGAT GATGATCGAT TTCCTCGGCG AAGGCGCGGG CCCCGCCCGT GAAGCGCACG ATGCGATCGT CGCGGCGATC GAGCACGTGC TGAAGCACGG CCCGCGCACG CGCGACATGG GCGGCCAGGC GTCGACAAGC GAAGTGGGCG ACGCGATCGT CGCGCGCCTG CTCGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T