ButhA.00088.a
Ornithine carbamoyltransferase
| CENTER ID: | ButhA.00088.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00088.a.A1.GE32409 | Full length( ButhA.00088.a ) | 1 | 309 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00088.a.A1.PS01339 | Full length( ButhA.00088.a ) | 1 | 309 |
Structures
4F2G
DEPOSITED: 5/7/2012
DETERMINATION: XRay
CLONE: ButhA.00088.a.A1.GE32409
PROTEIN: ButhA.00088.a.A1.PS01339
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127715 |
| PATRIC ID: | fig|271848.6.peg.3441 |
| RefSeq: | YP_441288.1 |
| UniProt: | Q2T0L1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDIIQRFAEN SRVPVINGLT NEYHPCQVLA DIFTYYEHRG PIRGKTVAWV GDANNMLYTW IQAARILDFK LQLSTPPGYA LDAKLVDAES APFYQVFDDP NEACKGADLV TTDVWTSMGF EAENEARKRA FADWCVDEEM MSHANSDALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRLNH
NT Sequence
ATGACCGCCA AAACCATTCG TCACTACCTG CAGTTCAAGG ATTTCTCGCT GGAAGACTAC GAGTACGTGC TCGAACGCAC GGGTATCCTG AAGCGCAAAT TCAAGAACTA CGAGACCTAT CACCCGCTGC ACGACCGCAC GCTCGCGATG ATCTTCGAGA AGAGCTCGAC GCGCACGCGC CTGTCGTTCG AGGCCGGGAT CTTTCAGCTA GGCGGCCACG CCGTGTTCAT GAGCACGCGC GACACGCAGC TCGGCCGCGG CGAACCCGTC GAGGATTCGG CGCAGGTGAT CTCGCGGATG GTCGACATCA TCATGATCCG CACGTTCGAG CAGGACATCA TCCAGCGCTT CGCCGAAAAT TCGCGCGTAC CGGTGATCAA TGGCCTGACG AACGAGTACC ACCCGTGCCA GGTGCTCGCC GACATCTTCA CGTATTACGA GCACCGCGGC CCGATTCGCG GCAAGACGGT CGCCTGGGTC GGCGACGCGA ACAACATGCT GTACACGTGG ATCCAGGCCG CGCGCATCCT CGACTTCAAG CTGCAGCTGT CGACGCCGCC CGGCTATGCG CTCGACGCGA AGCTCGTCGA TGCCGAAAGC GCGCCGTTCT ACCAGGTGTT CGACGATCCG AACGAAGCGT GCAAGGGTGC GGATCTCGTC ACGACCGACG TGTGGACGAG CATGGGCTTC GAGGCGGAGA ACGAGGCGCG CAAGCGGGCG TTCGCCGACT GGTGCGTCGA CGAGGAAATG ATGTCGCACG CGAATTCCGA CGCGCTCTTC ATGCACTGCC TGCCCGCGCA TCGCGGCGAG GAAGTAACGG CGGGCGTGAT CGACGGCCCG CAAAGCGTCG TGTGGGACGA GGCGGAAAAC CGTCTGCACG TGCAGAAGGC GCTCATGGAG TTCCTGTTGC TCGGGCGCCT GAACCAC
Details for ButhA.00088.a.A1.GE32409
| HARVESTED ON: | 11/8/2011 |
| SEQUENCED ON: | 11/11/2011 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDIIQRFAEN SRVPVINGLT NEYHPCQVLA DIFTYYEHRG PIRGKTVAWV GDANNMLYTW IQAARILDFK LQLSTPPGYA LDAKLVDAES APFYQVFDDP NEACKGADLV TTDVWTSMGF EAENEARKRA FADWCVDEEM MSHANSDALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRL
Validated NT Sequence
tcaggcgccc gagcaacagg aactccatga gcgccttctg cacgtgcaga cggttttccg cctcgtccca cacgacgctt tgcgggccgt cgatcacgcc cgccgttact tcctcgccgc gatgcgcggg caggcagtgc atgaagagcg cgtcggaatt cgcgtgcgac atcatttcct cgtcgacgca ccagtcggcg aacgcccgct tgcgcgcctc gttctccgcc tcgaagccca tgctcgtcca cacgtcggtc gtgacgagat ccgcaccctt gcacgcttcg ttcggatcgt cgaacacctg gtagaacggc gcgctttcgg catcgacgag cttcgcgtcg agcgcatagc cgggcggcgt cgacagctgc agcttgaagt cgaggatgcg cgcggcctgg atccacgtgt acagcatgtt gttcgcgtcg ccgacccagg cgaccgtctt gccgcgaatc gggccgcggt gctcgtaata cgtgaagatg tcggcgagca cctggcacgg gtggtactcg ttcgtcaggc cattgatcac cggtacgcgc gaattttcgg cgaagcgctg gatgatgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatcacctg cgccgaatcc tcgacgggtt cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac ggcgtggccg cctagctgaa agatcccggc ctcgaacgac aggcgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcggtc gtgcagcggg tgataggtct cgtagttctt gaatttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDIIQRFAE NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIRGKTVAW VGDANNMLYT WIQAARILDF KLQLSTPPGY ALDAKLVDAE SAPFYQVFDD PNEACKGADL VTTDVWTSMG FEAENEARKR AFADWCVDEE MMSHANSDAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLNH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccaaa accattcgtc actacctgca gttcaaggat ttctcgctgg aagactacga gtacgtgctc gaacgcacgg gtatcctgaa gcgcaaattc aagaactacg agacctatca cccgctgcac gaccgcacgc tcgcgatgat cttcgagaag agctcgacgc gcacgcgcct gtcgttcgag gccgggatct ttcagctagg cggccacgcc gtgttcatga gcacgcgcga cacgcagctc ggccgcggcg aacccgtcga ggattcggcg caggtgatct cgcggatggt cgacatcatc atgatccgca cgttcgagca ggacatcatc cagcgcttcg ccgaaaattc gcgcgtaccg gtgatcaatg gcctgacgaa cgagtaccac ccgtgccagg tgctcgccga catcttcacg tattacgagc accgcggccc gattcgcggc aagacggtcg cctgggtcgg cgacgcgaac aacatgctgt acacgtggat ccaggccgcg cgcatcctcg acttcaagct gcagctgtcg acgccgcccg gctatgcgct cgacgcgaag ctcgtcgatg ccgaaagcgc gccgttctac caggtgttcg acgatccgaa cgaagcgtgc aagggtgcgg atctcgtcac gaccgacgtg tggacgagca tgggcttcga ggcggagaac gaggcgcgca agcgggcgtt cgccgactgg tgcgtcgacg aggaaatgat gtcgcacgcg aattccgacg cgctcttcat gcactgcctg cccgcgcatc gcggcgagga agtaacggcg ggcgtgatcg acggcccgca aagcgtcgtg tgggacgagg cggaaaaccg tctgcacgtg cagaaggcgc tcatggagtt cctgttgctc gggcgcctga accactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.00088.a.A1.PS01339
| PURIFICATION DATe: | 1/10/2012 |
| CONCENTRATION: | 61.53mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDIIQRFAEN SRVPVINGLT NEYHPCQVLA DIFTYYEHRG PIRGKTVAWV GDANNMLYTW IQAARILDFK LQLSTPPGYA LDAKLVDAES APFYQVFDDP NEACKGADLV TTDVWTSMGF EAENEARKRA FADWCVDEEM MSHANSDALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRL
Validated NT Sequence
tcaggcgccc gagcaacagg aactccatga gcgccttctg cacgtgcaga cggttttccg cctcgtccca cacgacgctt tgcgggccgt cgatcacgcc cgccgttact tcctcgccgc gatgcgcggg caggcagtgc atgaagagcg cgtcggaatt cgcgtgcgac atcatttcct cgtcgacgca ccagtcggcg aacgcccgct tgcgcgcctc gttctccgcc tcgaagccca tgctcgtcca cacgtcggtc gtgacgagat ccgcaccctt gcacgcttcg ttcggatcgt cgaacacctg gtagaacggc gcgctttcgg catcgacgag cttcgcgtcg agcgcatagc cgggcggcgt cgacagctgc agcttgaagt cgaggatgcg cgcggcctgg atccacgtgt acagcatgtt gttcgcgtcg ccgacccagg cgaccgtctt gccgcgaatc gggccgcggt gctcgtaata cgtgaagatg tcggcgagca cctggcacgg gtggtactcg ttcgtcaggc cattgatcac cggtacgcgc gaattttcgg cgaagcgctg gatgatgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatcacctg cgccgaatcc tcgacgggtt cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac ggcgtggccg cctagctgaa agatcccggc ctcgaacgac aggcgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcggtc gtgcagcggg tgataggtct cgtagttctt gaatttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDIIQRFAE NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIRGKTVAW VGDANNMLYT WIQAARILDF KLQLSTPPGY ALDAKLVDAE SAPFYQVFDD PNEACKGADL VTTDVWTSMG FEAENEARKR AFADWCVDEE MMSHANSDAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLNH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCAAA ACCATTCGTC ACTACCTGCA GTTCAAGGAT TTCTCGCTGG AAGACTACGA GTACGTGCTC GAACGCACGG GTATCCTGAA GCGCAAATTC AAGAACTACG AGACCTATCA CCCGCTGCAC GACCGCACGC TCGCGATGAT CTTCGAGAAG AGCTCGACGC GCACGCGCCT GTCGTTCGAG GCCGGGATCT TTCAGCTAGG CGGCCACGCC GTGTTCATGA GCACGCGCGA CACGCAGCTC GGCCGCGGCG AACCCGTCGA GGATTCGGCG CAGGTGATCT CGCGGATGGT CGACATCATC ATGATCCGCA CGTTCGAGCA GGACATCATC CAGCGCTTCG CCGAAAATTC GCGCGTACCG GTGATCAATG GCCTGACGAA CGAGTACCAC CCGTGCCAGG TGCTCGCCGA CATCTTCACG TATTACGAGC ACCGCGGCCC GATTCGCGGC AAGACGGTCG CCTGGGTCGG CGACGCGAAC AACATGCTGT ACACGTGGAT CCAGGCCGCG CGCATCCTCG ACTTCAAGCT GCAGCTGTCG ACGCCGCCCG GCTATGCGCT CGACGCGAAG CTCGTCGATG CCGAAAGCGC GCCGTTCTAC CAGGTGTTCG ACGATCCGAA CGAAGCGTGC AAGGGTGCGG ATCTCGTCAC GACCGACGTG TGGACGAGCA TGGGCTTCGA GGCGGAGAAC GAGGCGCGCA AGCGGGCGTT CGCCGACTGG TGCGTCGACG AGGAAATGAT GTCGCACGCG AATTCCGACG CGCTCTTCAT GCACTGCCTG CCCGCGCATC GCGGCGAGGA AGTAACGGCG GGCGTGATCG ACGGCCCGCA AAGCGTCGTG TGGGACGAGG CGGAAAACCG TCTGCACGTG CAGAAGGCGC TCATGGAGTT CCTGTTGCTC GGGCGCCTGA ACCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA