ButhA.00080.a

30S ribosomal protein S12 (BTH_I3073) (rpsL)

CENTER ID: ButhA.00080.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00080.a.A1.GE32786 full length 1 126
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5964
RefSeq: YP_443577.1
UniProt: Q2SU22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTINQLVRK GRASETTKSK SPALQDCPQR RGVCTRVYTT TPKKPNSALR KVAKVRLTNG FEVISYIGGE GHNLQEHSVV LIRGGRVKDL PGVRYHMVRG SLDTQGVKDR KQARSKYGAK RAKAAK
NT Sequence
atgccaacca tcaatcaact ggttcgcaaa ggccgcgcgt cggaaacgac gaagagcaag agcccggcct tgcaggactg cccccagcgt cgcggcgtgt gcacccgtgt gtacacgacg acgccgaaga agccgaactc ggcactccgt aaggttgcca aggttcgtct gacgaacggc ttcgaagtga tttcgtacat cggcggtgaa ggccacaacc tgcaggagca ctcggttgtg ctgatccgcg gcggccgtgt gaaggacttg ccgggtgtgc gttatcacat ggttcgcggc tcgctggata cgcagggcgt caaggaccgt aagcaggctc gctcgaagta cggcgcgaag cgtgcgaagg ctgcgaagta a
Details for ButhA.00080.a.A1.GE32786
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 16kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPTINQLVR KGRASETTKS KSPALQDCPQ RRGVCTRVYT TTPKKPNSAL RKVAKVRLTN GFEVISYIGG EGHNLQEHSV VLIRGGRVKD LPGVRYHMVR GSLDTQGVKD RKQARSKYGA KRAKAAK
Validated NT Sequence
ttttnttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgccaac catcaatcaa ctggttcgca aaggccgcgc gtcggaaacg acgaagagca agagcccggc cttgcaggac tgcccccagc gtcgcggcgt gtgcacccgt gtgtacacga cgacgccgaa gaagccgaac tcggcactcc gtaaggttgc caaggttcgt ctgacgaacg gcttcgaagt gatttcgtac atcggcggtg aaggccacaa cctgcaggag cactcggttg tgctgatccg cggcggccgt gtgaaggact tgccgggtgt gcgttatcac atggttcgcg gctcgctgga tacgcagggc gtcaaggacc gtaagcaggc tcgctcgaag tacggcgcga agcgtgcgaa ggctgcgaag taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgcnnnnn naaannnnnn nnanaaaann nnnnnnnnnn nnnnnnnntn cnacngnnnn tnnnnnnnan gctcnnggnn nncnnnnnnn nnnnnnnnnn gcncntcnnc ggcnacnncn ncnagannnn nnnnnnntnc gtcnnccnca ncnncnnnat gctgatcnnc nnnannnngn ngnnncnnnn nnnnnnnnnn nnggntccng nntcnnnnnn ngaannanan gcnnnnnnnn ggncnnnncc tgnnnntntc nnngcngctn cnannncnnn nnnnnnnnnc gnnnnannnc nnanccgnng ancnnnnnng ncncnnnnnn nancnnnntc nnncnnncnn cgnannncga nncnnnnncn nnnagnnnnn nnngangcnc ncnctncnnn nnnncnnnan nnnnnncnnn nnnnnnancn nnnnnnnnnn nnnnnnccnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnnnn nnntnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPTINQLVR KGRASETTKS KSPALQDCPQ RRGVCTRVYT TTPKKPNSAL RKVAKVRLTN GFEVISYIGG EGHNLQEHSV VLIRGGRVKD LPGVRYHMVR GSLDTQGVKD RKQARSKYGA KRAKAAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccaaccatc aatcaactgg ttcgcaaagg ccgcgcgtcg gaaacgacga agagcaagag cccggccttg caggactgcc cccagcgtcg cggcgtgtgc acccgtgtgt acacgacgac gccgaagaag ccgaactcgg cactccgtaa ggttgccaag gttcgtctga cgaacggctt cgaagtgatt tcgtacatcg gcggtgaagg ccacaacctg caggagcact cggttgtgct gatccgcggc ggccgtgtga aggacttgcc gggtgtgcgt tatcacatgg ttcgcggctc gctggatacg cagggcgtca aggaccgtaa gcaggctcgc tcgaagtacg gcgcgaagcg tgcgaaggct gcgaagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa