SSGCID: Target Page

ButhA.00066.a

DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) (EC 2.7.7.6) (RNA polymerase subunit alpha) (Transcriptase subunit alpha) (BTH_I3042) (rpoA)

CENTER ID:	ButhA.00066.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00066.a.A1.GE32715	full length		1	325

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00066.a.A1.PS01170	full length		1	325

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5933
RefSeq:	YP_443546.1
UniProt:	Q2SU53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQTSLLKPKI IAVESLGENH AKVVMEPFER GYGHTLGNAL RRVLLSSMVG YAPTEVTIAG VVHEYSTLDG VQEDVVNLLL NLKGVVFKLH NRDEVTVTLR KEGEGVVTAG DIELAHDCEV INPNHVIAHL SKGGKLDVQI KVEKGRGYVP GNVRRYGDET AKIIGRIVLD ASFSPVRRVS YAVESARVEQ RTDLDKLVMN IETSGVITPE EAIRQSARIL VDQLSVFAAL EGTETAAEAP SRAPQIDPIL LRPVDDLELT VRSANCLKAE NIYYIGDLIQ RTENELLKTP NLGRKSLNEI KEVLASRGLT LGMKLENWPP AGLDK

NT Sequence

atgcaaacca gtttgttgaa acccaagatc atcgccgtgg aatctctggg cgagaaccac gcgaaggtgg tcatggagcc gttcgagcgc ggttacggcc acaccttggg caatgcgctt cgccgtgtgc tgctgtcttc gatggtcggc tacgcgccga ccgaagtgac gatcgccggc gtcgtgcacg agtattcgac gcttgatggt gtgcaagagg acgtcgtcaa cctgctgctg aacctgaagg gcgtggtgtt caagctgcat aaccgcgacg aagtcacggt gacgctgcgc aaggaaggcg aaggcgtcgt gacggccggc gatatcgaac tggcccacga ctgcgaagtc atcaacccga atcacgtgat cgcgcacctg tcgaagggcg gcaagctcga cgtgcagatc aaggtcgaga agggccgcgg ctatgtgccg ggcaacgtgc gccgctacgg cgacgaaacg gcgaagatca tcggccgcat cgtcctcgac gcatcgttct cgccggttcg ccgcgtgagc tacgcggtcg agagcgcgcg tgtcgagcag cgtaccgatc tcgacaagct cgtgatgaac atcgaaacga gcggcgtcat cacgccggaa gaagcgattc gccagtcggc tcgcatcctt gtcgaccaac tgtccgtgtt cgcggcgctg gaaggcacgg aaacggccgc ggaagcgccg tcgcgcgcgc cgcagatcga tccgatcctg ctgcgtccgg tcgacgacct cgagctgacc gtccgttcgg cgaactgctt gaaggcggag aacatctact acatcggcga tctgatccag cgcaccgaaa acgagctgct gaagacgccg aatctcggcc gcaagtcgct caacgagatc aaggaagtgc tcgcttcgcg cggtctcacg ctgggcatga agcttgagaa ctggccgccg gctggtctcg acaagtaa

Details for ButhA.00066.a.A1.GE32715

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	37kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMQTSLLK PKIIAVESLG ENHAKVVMEP FERGYGHTLG NALRRVLLSS MVGYAPTEVT IAGVVHEYST LDGVQEDVVN LLLNLKGVVF KLHNRDEVTV TLRKEGEGVV TAGDIELAHD CEVINPNHVI AHLSKGGKLD VQIKVEKGRG YVPGNVRRYG DETAKIIGRI VLDASFSPVR RVSYAVESAR VEQRTDLDKL VMNIETSGVI TPEEAIRQSA RILVDQLSVF AALEGTETAA EAPSRAPQID PILLRPVDDL ELTVRSANCL KAENIYYIGD LIQRTENELL KTPNLGRKSL NEIKEVLASR GLTLGMKLEN WPPAGL

Validated NT Sequence

ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc aaaccagttt gttgaaaccc aagatcatcg ccgtggaatc tctgggcgag aaccacgcga aggtggtcat ggagccgttc gagcgcggtt acggccacac cttgggcaat gcgcttcgcc gtgtgctgct gtcttcgatg gtcggctacg cgccgaccga agtgacgatc gccggcgtcg tgcacgagta ttcgacgctt gatggtgtgc aagaggacgt cgtcaacctg ctgctgaacc tgaagggcgt ggtgttcaag ctgcataacc gcgacgaagt cacggtgacg ctgcgcaagg aaggcgaagg cgtcgtgacg gccggcgata tcgaactggc ccacgactgc gaagtcatca acccgaatca cgtgatcgcg cacctgtcga agggcggcaa gctcgacgtg cagatcaagg tcgagaaggg ccgcggctat gtgccgggca acgtgcgccg ctacggcgac gaaacggcga agatcatcgg ccgcatcgtc ctcgacgcat cgttctcgcc ggttcgccgc gtgagctacg cggtcgagag cgcgcgtgtc gagcagcgta ccgatctcga caagctcgtg atgaacatcg aaacgagcgg cgtcatcacg ccggaagaag cgattcgcca gtcggctcgc atccttgtcg accaactgtc cgtgttcgcg gcgctggaag gcacggaaac ggccgcggaa gcgccgtcgc gcgcgccgca gatcgatccg atcctgctgc gtccggtcga cgacctcgag ctgaccgtcc gttcggcgaa ctgcttgaag gcggagaaca tctactacat cggcgatctg atccagcgca ccgaaaacga gctgctgaag acgccgaatc tcggccgcaa gtcgctcaac gagatcaagg aagtgctcgc ttcgcgcggt ctcacgctgg gcatgaagct tgagaactgg ccgccggctg gtctcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQTSLLKPK IIAVESLGEN HAKVVMEPFE RGYGHTLGNA LRRVLLSSMV GYAPTEVTIA GVVHEYSTLD GVQEDVVNLL LNLKGVVFKL HNRDEVTVTL RKEGEGVVTA GDIELAHDCE VINPNHVIAH LSKGGKLDVQ IKVEKGRGYV PGNVRRYGDE TAKIIGRIVL DASFSPVRRV SYAVESARVE QRTDLDKLVM NIETSGVITP EEAIRQSARI LVDQLSVFAA LEGTETAAEA PSRAPQIDPI LLRPVDDLEL TVRSANCLKA ENIYYIGDLI QRTENELLKT PNLGRKSLNE IKEVLASRGL TLGMKLENWP PAGLDK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaaccagt ttgttgaaac ccaagatcat cgccgtggaa tctctgggcg agaaccacgc gaaggtggtc atggagccgt tcgagcgcgg ttacggccac accttgggca atgcgcttcg ccgtgtgctg ctgtcttcga tggtcggcta cgcgccgacc gaagtgacga tcgccggcgt cgtgcacgag tattcgacgc ttgatggtgt gcaagaggac gtcgtcaacc tgctgctgaa cctgaagggc gtggtgttca agctgcataa ccgcgacgaa gtcacggtga cgctgcgcaa ggaaggcgaa ggcgtcgtga cggccggcga tatcgaactg gcccacgact gcgaagtcat caacccgaat cacgtgatcg cgcacctgtc gaagggcggc aagctcgacg tgcagatcaa ggtcgagaag ggccgcggct atgtgccggg caacgtgcgc cgctacggcg acgaaacggc gaagatcatc ggccgcatcg tcctcgacgc atcgttctcg ccggttcgcc gcgtgagcta cgcggtcgag agcgcgcgtg tcgagcagcg taccgatctc gacaagctcg tgatgaacat cgaaacgagc ggcgtcatca cgccggaaga agcgattcgc cagtcggctc gcatccttgt cgaccaactg tccgtgttcg cggcgctgga aggcacggaa acggccgcgg aagcgccgtc gcgcgcgccg cagatcgatc cgatcctgct gcgtccggtc gacgacctcg agctgaccgt ccgttcggcg aactgcttga aggcggagaa catctactac atcggcgatc tgatccagcg caccgaaaac gagctgctga agacgccgaa tctcggccgc aagtcgctca acgagatcaa ggaagtgctc gcttcgcgcg gtctcacgct gggcatgaag cttgagaact ggccgccggc tggtctcgac aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.00066.a.A1.PS01170

PURIFICATION DATe:	8/4/2011
CONCENTRATION:	68mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAACCAGT TTGTTGAAAC CCAAGATCAT CGCCGTGGAA TCTCTGGGCG AGAACCACGC GAAGGTGGTC ATGGAGCCGT TCGAGCGCGG TTACGGCCAC ACCTTGGGCA ATGCGCTTCG CCGTGTGCTG CTGTCTTCGA TGGTCGGCTA CGCGCCGACC GAAGTGACGA TCGCCGGCGT CGTGCACGAG TATTCGACGC TTGATGGTGT GCAAGAGGAC GTCGTCAACC TGCTGCTGAA CCTGAAGGGC GTGGTGTTCA AGCTGCATAA CCGCGACGAA GTCACGGTGA CGCTGCGCAA GGAAGGCGAA GGCGTCGTGA CGGCCGGCGA TATCGAACTG GCCCACGACT GCGAAGTCAT CAACCCGAAT CACGTGATCG CGCACCTGTC GAAGGGCGGC AAGCTCGACG TGCAGATCAA GGTCGAGAAG GGCCGCGGCT ATGTGCCGGG CAACGTGCGC CGCTACGGCG ACGAAACGGC GAAGATCATC GGCCGCATCG TCCTCGACGC ATCGTTCTCG CCGGTTCGCC GCGTGAGCTA CGCGGTCGAG AGCGCGCGTG TCGAGCAGCG TACCGATCTC GACAAGCTCG TGATGAACAT CGAAACGAGC GGCGTCATCA CGCCGGAAGA AGCGATTCGC CAGTCGGCTC GCATCCTTGT CGACCAACTG TCCGTGTTCG CGGCGCTGGA AGGCACGGAA ACGGCCGCGG AAGCGCCGTC GCGCGCGCCG CAGATCGATC CGATCCTGCT GCGTCCGGTC GACGACCTCG AGCTGACCGT CCGTTCGGCG AACTGCTTGA AGGCGGAGAA CATCTACTAC ATCGGCGATC TGATCCAGCG CACCGAAAAC GAGCTGCTGA AGACGCCGAA TCTCGGCCGC AAGTCGCTCA ACGAGATCAA GGAAGTGCTC GCTTCGCGCG GTCTCACGCT GGGCATGAAG CTTGAGAACT GGCCGCCGGC TGGTCTCGAC AAGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA