ButhA.00063.a
Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) (BTH_I2235) (hisS)
| CENTER ID: | ButhA.00063.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00063.a.A1.GE32714 | full length | 1 | 446 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4E51
DEPOSITED: 3/13/2012
DETERMINATION: XRay
CLONE: ButhA.00063.a.A1.GE32714
PROTEIN: ButhA.00063.a.A1.PS01164
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126806 |
| PATRIC ID: | fig|271848.6.peg.5055 |
| RefSeq: | YP_442756.1 |
| UniProt: | Q2SWE3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEQKRKLEK LTGVKGMNDI LPQDAGLWEF FEATVKSLLR AYGYQNIRTP IVEHTPLFTR GIGEVTDIVE KEMYSFVDAL NGENLTLRPE NTAAVVRAAI EHNMLYDGPK RLWYIGPMFR HERPQRGRYR QFHQVGVEAL GFAGPDADAE IVMMCQRLWE DLGLTGIKLE INSLGLAEER AAHRVELIKY LEQHADKLDD DAQRRLYTNP LRVLDTKNPA LQEIVRNAPK LIDFLGDVSR AHFEGLQRLL KANNVPFTIN PRLVRGLDYY NLTVFEWVTD KLGAQGTVAA GGRYDPLIEQ LGGKPTAACG WAMGIERILE LLKEEHLVPE QEGVDVYVVH QGDAAREQAF IVAERLRDTG LDVILHCSAD GAGASFKSQM KRADASGAAF AVIFGEDEVT NGTASVKPLR GTGDDGEKSV QQSVPVESLT EFLINAMVAT AEDGDD
NT Sequence
atgacagaac aaaagcgaaa gctcgagaag ctgacgggcg tgaagggcat gaacgacatc ctcccgcagg atgccggctt gtgggaattc ttcgaagcga cggtgaagtc gctgttgcgc gcatacggct atcagaatat ccgcacgccg atcgtcgagc acacgccgct ctttacgcgc ggcatcggcg aagtgaccga catcgtcgaa aaggaaatgt acagctttgt cgacgcgctc aacggcgaga acctgacgct gcgtcccgag aacaccgcgg ccgtcgtgcg cgcggcgatc gagcacaaca tgctgtacga cggtccgaag cgactgtggt acatcgggcc gatgttccgt cacgagcgcc cgcagcgcgg ccgctatcgc cagttccatc aggtcggcgt cgaggcgctc ggcttcgcgg gcccggacgc ggacgcggaa atcgtcatga tgtgccagcg cctgtgggag gatctcggcc tcaccggcat caagctggag atcaactcgc tcggtctcgc cgaggagcgc gccgcgcacc gcgtcgagct catcaagtat ctcgagcagc atgcggacaa gctcgacgac gacgcgcagc gccgcctcta cacgaatccg ctgcgtgtgc tcgacacgaa gaaccccgcg ctgcaggaga tcgtgcggaa cgcgccgaaa ctgatcgact tcctcggcga cgtgtcgcgc gcgcacttcg aagggctgca acggctgctg aaggcgaaca acgtgccgtt cacgatcaat ccgcgcctcg tgcgcgggct cgattattac aacctgaccg tgttcgagtg ggtgaccgac aagctcggcg cgcaaggcac ggtcgccgcg ggcggccgct acgatccgct gatcgagcaa ctgggcggca agccgaccgc cgcgtgcggc tgggcgatgg gcatcgagcg catccttgag cttctgaagg aagagcatct cgtgccggag caggaaggcg tcgacgtgta cgtcgtccac cagggcgacg ccgcgcgcga gcaggcgttc atcgtcgccg agcgtctgcg cgacacgggc ctcgacgtga tcctgcattg cagcgcggac ggcgcgggcg cgagcttcaa gtcgcagatg aagcgcgcgg acgcgagcgg tgcggcgttc gcggtgatct tcggcgaaga cgaggtcaca aacggcacgg cgagcgtgaa gccgctgcgc ggcacgggcg acgacggcga aaagagcgtt cagcagtccg tgccggtcga gagcttgacc gaatttctaa tcaatgcgat ggttgcaacc gccgaagacg gcgacgactg a
Details for ButhA.00063.a.A1.GE32714
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTEQKRK LEKLTGVKGM NDILPQDAGL WEFFEATVKS LLRAYGYQNI RTPIVEHTPL FTRGIGEVTD IVEKEMYSFV DALNGENLTL RPENTAAVVR AAIEHNMLYD GPKRLWYIGP MFRHERPQRG RYRQFHQVGV EALGFAGPDA DAEIVMMCQR LWEDLGLTGI KLEINSLGLA EERAAHRVEL IKYLEQHADK LDDDAQRRLY TNPLRVLDTK NPALQEIVRN APKLIDFLGD VSRAHFEGLQ RLLKANNVPF TINPRLVRGL DYYNLTVFEW VTDKLGAQGT VAAGGRYDPL IEQLGGKPTA ACGWAMGIER ILELLKEEHL VPEQEGVDVY VVHQGDAARE QAFIVAERLR DTGLDVILHC SADGAGASFK SQMKRADASG AAFAVIFGED EVTNGTASVK PLRGTGDDGE KSVQQSVPVE SLTEFLINAM VATAEDG
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacaga acaaaagcga aagctcgaga agctgacggg cgtgaagggc atgaacgaca tcctcccgca ggatgccggc ttgtgggaat tcttcgaagc gacggtgaag tcgctgttgc gcgcatacgg ctatcagaat atccgcacgc cgatcgtcga gcacacgccg ctctttacgc gcggcatcgg cgaagtgacc gacatcgtcg aaaaggaaat gtacagcttt gtcgacgcgc tcaacggcga gaacctgacg ctgcgtcccg agaacaccgc ggccgtcgtg cgcgcggcga tcgagcacaa catgctgtac gacggtccga agcgactgtg gtacatcggg ccgatgttcc gtcacgagcg cccgcagcgc ggccgctatc gccagttcca tcaggtcggc gtcgaggcgc tcggcttcgc gggcccggac gcggacgcgg aaatcgtcat gatgtgccag cgcctgtggg aggatctcgg cctcaccggc atcaagctgg agatcaactc gctcggtctc gccgaggagc gcgccgcgca ccgcgtcgag ctcatcaagt atctcgagca gcatgcggac aagctcgacg acgacgcgca gcgccgcctc tacacgaatc cgctgcgtgt gctcgacacg aagaaccccg cgctgcagga gatcgtgcgg aacgcgccga aactgatcga cttcctcggc gacgtgtcgc gcgcgcactt cgaagggctg caacggctgc tgaaggcgaa caacgtgccg ttcacgatca atccgcgcct cgtgcgcggg ctcgattatt acaacctgac cgtgttcgag tgggtgaccg acaagctcgg cgcgcaaggc acggtcgccg cgggcggccg ctacgatccg ctgatcgagc aactgggcgg caagccgacc gccgcgtgcg gctgggcgat gggcatcgag cgcatccttg agcttctgaa ggaagagcat ctcgtgccgg agcaggaagg cgtcgacgtg tacgtcgtcc accagggcga cgccgcgcgc gagcaggcgt tcatcgtcgc cgagcgtctg cgcgacacgg gcctcgacgt gatcctgcat tgcagcgcgg acggcgcggg cgcgagcttc aagtcgcaga tgaagcgcgc ggacgcgagc ggtgcggcgt tcgcggtgat cttcggcgaa gacgaggtca caaacggcac ggcgagcgtg aagccgctgc gcggcacggg cgacgacggc gaaaagagcg ttcagcagtc cgtgccggtc gagagcttga ccgaatttct aatcaatgcg atggttgcaa ccgccgaaga cggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEQKRKLE KLTGVKGMND ILPQDAGLWE FFEATVKSLL RAYGYQNIRT PIVEHTPLFT RGIGEVTDIV EKEMYSFVDA LNGENLTLRP ENTAAVVRAA IEHNMLYDGP KRLWYIGPMF RHERPQRGRY RQFHQVGVEA LGFAGPDADA EIVMMCQRLW EDLGLTGIKL EINSLGLAEE RAAHRVELIK YLEQHADKLD DDAQRRLYTN PLRVLDTKNP ALQEIVRNAP KLIDFLGDVS RAHFEGLQRL LKANNVPFTI NPRLVRGLDY YNLTVFEWVT DKLGAQGTVA AGGRYDPLIE QLGGKPTAAC GWAMGIERIL ELLKEEHLVP EQEGVDVYVV HQGDAAREQA FIVAERLRDT GLDVILHCSA DGAGASFKSQ MKRADASGAA FAVIFGEDEV TNGTASVKPL RGTGDDGEKS VQQSVPVESL TEFLINAMVA TAEDGDD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagaacaa aagcgaaagc tcgagaagct gacgggcgtg aagggcatga acgacatcct cccgcaggat gccggcttgt gggaattctt cgaagcgacg gtgaagtcgc tgttgcgcgc atacggctat cagaatatcc gcacgccgat cgtcgagcac acgccgctct ttacgcgcgg catcggcgaa gtgaccgaca tcgtcgaaaa ggaaatgtac agctttgtcg acgcgctcaa cggcgagaac ctgacgctgc gtcccgagaa caccgcggcc gtcgtgcgcg cggcgatcga gcacaacatg ctgtacgacg gtccgaagcg actgtggtac atcgggccga tgttccgtca cgagcgcccg cagcgcggcc gctatcgcca gttccatcag gtcggcgtcg aggcgctcgg cttcgcgggc ccggacgcgg acgcggaaat cgtcatgatg tgccagcgcc tgtgggagga tctcggcctc accggcatca agctggagat caactcgctc ggtctcgccg aggagcgcgc cgcgcaccgc gtcgagctca tcaagtatct cgagcagcat gcggacaagc tcgacgacga cgcgcagcgc cgcctctaca cgaatccgct gcgtgtgctc gacacgaaga accccgcgct gcaggagatc gtgcggaacg cgccgaaact gatcgacttc ctcggcgacg tgtcgcgcgc gcacttcgaa gggctgcaac ggctgctgaa ggcgaacaac gtgccgttca cgatcaatcc gcgcctcgtg cgcgggctcg attattacaa cctgaccgtg ttcgagtggg tgaccgacaa gctcggcgcg caaggcacgg tcgccgcggg cggccgctac gatccgctga tcgagcaact gggcggcaag ccgaccgccg cgtgcggctg ggcgatgggc atcgagcgca tccttgagct tctgaaggaa gagcatctcg tgccggagca ggaaggcgtc gacgtgtacg tcgtccacca gggcgacgcc gcgcgcgagc aggcgttcat cgtcgccgag cgtctgcgcg acacgggcct cgacgtgatc ctgcattgca gcgcggacgg cgcgggcgcg agcttcaagt cgcagatgaa gcgcgcggac gcgagcggtg cggcgttcgc ggtgatcttc ggcgaagacg aggtcacaaa cggcacggcg agcgtgaagc cgctgcgcgg cacgggcgac gacggcgaaa agagcgttca gcagtccgtg ccggtcgaga gcttgaccga atttctaatc aatgcgatgg ttgcaaccgc cgaagacggc gacgactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa