SSGCID: Target Page

ButhA.00062.b

Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) (BTH_I2056) (guaB)

CENTER ID:	ButhA.00062.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00062.b.A1.GE32713	full length		1	486

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00062.b.A1.PS01167	full length		1	486

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4868
RefSeq:	YP_442580.1
UniProt:	Q2SWW9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLIQKALTF DDVLLVPAFS DVLPRDTSLK TQLTRHISLN MPLVSAAMDT VTEGRLAIAM AQQGGVGIVH KNLTPVEQAR EVAKVKRFES GVVRDPITVP PSMKVRDVIA LSRQHGISGF PVLEGPKLVG IVTNRDLRFE TRLDEPVKSI MTPRERLVTV AEGTPLAEAK ALMHSHRLER VLVVNDAFEL RGLMTVKDIT KQTEHPEACK DEHGKLRVGA AVGVGADNEE RVELLVQAGV DVIVVDTAHG HSKGVLERVR WVKQNFPKVE VIGGNIATAA AAKALVEYGA DAVKVGIGPG SICTTRIVAG VGVPQISAIA NVSDALRGTG VPCIADGGIR FSGDVSKALA AGANAVMMGS MFAGTEEAPG DVFLYQGRQY KSYRGMGSVG AMKDGAADRY FQDNSANIDK LVPEGIEGRV AYKGSVNAII FQLIGGVRAS MGYCGCKTIA EMHEKAEFVE ITAAGMRESH VHDVQITKEA PNYHVD

NT Sequence

atgcgtctga tccaaaaagc actcacgttc gatgacgtgc tcctcgtccc ggcgttctcc gacgttctgc cgcgcgacac cagcctcaag acccagctga cccgccacat ctccctgaac atgccgctcg tgtccgccgc gatggacacg gtcaccgaag gccgcctcgc gatcgcgatg gcgcagcaag gcggcgtcgg catcgtccac aagaatctca cccccgtcga gcaggcgcgc gaggtcgcga aggtcaagcg cttcgaatcg ggcgtcgtgc gcgatccgat caccgtcccg ccgagcatga aggtgcgcga cgtgatcgcg ctgtcgcgcc agcatggcat ctcgggtttc ccggtcctcg aaggcccgaa gctcgtcggc atcgtgacga accgcgacct gcgcttcgaa acccgcctcg acgagccggt caagtcgatc atgacgccgc gcgagcgcct cgtgacggtt gccgaaggca cgccgctcgc cgaggcgaag gcgctgatgc acagccaccg cctcgagcgc gtgctcgtcg tcaacgatgc gttcgagctg cgcggcctga tgacggtcaa ggacatcacg aagcagaccg agcatccgga agcgtgcaag gacgagcacg gcaagctgcg cgtcggcgcc gcggtcggcg tgggggcgga caacgaggag cgcgtcgagc tgctcgtgca ggcgggcgtc gacgtgatcg tcgtcgacac cgcgcacggc cacagcaagg gcgtgctcga gcgcgtgcgc tgggtcaagc agaacttccc gaaggtcgag gtgatcggcg gcaacatcgc gaccgcggcc gccgcgaagg cgctcgtcga atacggcgcg gacgcggtga aggtcggcat cggcccgggc tcgatctgca cgacgcggat cgtcgcgggc gtcggcgtgc cgcagatcag cgcgatcgcg aacgtgtcgg acgcgttgcg cggcaccggc gtgccgtgca tcgccgacgg cggcatccgc ttctcgggcg acgtgtcgaa ggcgctcgcc gccggcgcga acgcggtgat gatgggcagc atgttcgcgg gcaccgaaga ggcgccgggc gacgtgttcc tgtaccaggg ccgccagtac aagtcgtatc gcggcatggg ctcggtcggc gcgatgaagg acggcgcggc ggaccgctac ttccaggaca actccgcgaa catcgacaag ctcgtgccgg aaggcatcga aggccgcgtc gcgtacaagg gctcggtcaa cgcgatcatc ttccagctga tcggcggcgt gcgggcgagc atgggctact gcggctgcaa gacgatcgcc gagatgcacg agaaggccga gttcgtcgag atcaccgcgg ccggcatgcg cgagtcgcac gtgcacgacg tgcagatcac gaaggaagcc cccaactatc acgtggactg a

Details for ButhA.00062.b.A1.GE32713

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRHISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVLEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEXTPLAEA XALMHSHRLE RVLVVXDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPKV EVIGGNIATA AAAKALVEYG ADAVKVGIGP GSICTTRIVA GVGVPQISAI ANVSDALRGT GVPCIADGGI RFSGDVSKAL AAGANAVMMG SMFAGTEEAP GDVFLYQGRQ YKSYRGMGSV GAMKDGAADR YFQDNSANID KLVPEGIEGR VAYKGSVNAI IFQLIGGVRA SMGYCGCKTI AEMHEKAEFV EITAAGMRES HVHDVQITKE APNYH

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtc tgatccaaaa agcactcacg ttcgatgacg tgctcctcgt cccggcgttc tccgacgttc tgccgcgcga caccagcctc aagacccagc tgacccgcca catctccctg aacatgccgc tcgtgtccgc cgcgatggac acggtcaccg aaggccgcct cgcgatcgcg atggcgcagc aaggcggcgt cggcatcgtc cacaagaatc tcacccccgt cgagcaggcg cgcgaggtcg cgaaggtcaa gcgcttcgaa tcgggcgtcg tgcgcgatcc gatcaccgtc ccgccgagca tgaaggtgcg cgacgtgatc gcgctgtcgc gccagcatgg catctcgggt ttcccggtcc tcgaaggccc gaagctcgtc ggcatcgtga cgaaccgcga cctgcgcttc gaaacccgcc tcgacgagcc ggtcaagtcg atcatgacgc cgcgcgagcg cctcgtgacg gttgccgaan gcacgccgct cgccgaggcg aangcgctga tgcacagcca ccgcctcgag cgcgtgctcg tcgtcnacga tgcgttcgag ctgcgcggcc tgatgacggt caaggacatc acgaagcaga ccgagcatcc ggaagcgtgc aaggacgagc acggcaagct gcgcgtcggc gccgcggtcg gcgtgggggc ggacaacgag gagcgcgtcg agctgctcgt gcaggcgggc gtcgacgtga tcgtcgtcga caccgcgcac ggccacagca agggcgtgct cgagcgcgtg cgctgggtca agcagaactt cccgaaggtc gaggtgatcg gcggcaacat cgcgaccgcg gccgccgcga aggcgctcgt cgaatacggc gcggacgcgg tgaaggtcgg catcggcccg ggctcgatct gcacgacgcg gatcgtcgcg ggcgtcggcg tgccgcagat cagcgcgatc gcgaacgtgt cggacgcgtt gcgcggcacc ggcgtgccgt gcatcgccga cggcggcatc cgcttctcgg gcgacgtgtc gaaggcgctc gccgccggcg cgaacgcggt gatgatgggc agcatgttcg cgggcaccga agaggcgccg ggcgacgtgt tcctgtacca gggccgccag tacaagtcgt atcgcggcat gggctcggtc ggcgcgatga aggacggcgc ggcggaccgc tacttccagg acaactccgc gaacatcgac aagctcgtgc cggaaggcat cgaaggccgc gtcgcgtaca agggctcggt caacgcgatc atcttccagc tgatcggcgg cgtgcgggcg agcatgggct actgcggctg caagacgatc gccgagatgc acgagaaggc cgagttcgtc gagatcaccg cggccggcat gcgcgagtcg cacgtgcacg acgtgcagat cacgaaggaa gcccccaact atcacgtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRHISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVLEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEGTPLAEA KALMHSHRLE RVLVVNDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPKV EVIGGNIATA AAAKALVEYG ADAVKVGIGP GSICTTRIVA GVGVPQISAI ANVSDALRGT GVPCIADGGI RFSGDVSKAL AAGANAVMMG SMFAGTEEAP GDVFLYQGRQ YKSYRGMGSV GAMKDGAADR YFQDNSANID KLVPEGIEGR VAYKGSVNAI IFQLIGGVRA SMGYCGCKTI AEMHEKAEFV EITAAGMRES HVHDVQITKE APNYHVD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctgatc caaaaagcac tcacgttcga tgacgtgctc ctcgtcccgg cgttctccga cgttctgccg cgcgacacca gcctcaagac ccagctgacc cgccacatct ccctgaacat gccgctcgtg tccgccgcga tggacacggt caccgaaggc cgcctcgcga tcgcgatggc gcagcaaggc ggcgtcggca tcgtccacaa gaatctcacc cccgtcgagc aggcgcgcga ggtcgcgaag gtcaagcgct tcgaatcggg cgtcgtgcgc gatccgatca ccgtcccgcc gagcatgaag gtgcgcgacg tgatcgcgct gtcgcgccag catggcatct cgggtttccc ggtcctcgaa ggcccgaagc tcgtcggcat cgtgacgaac cgcgacctgc gcttcgaaac ccgcctcgac gagccggtca agtcgatcat gacgccgcgc gagcgcctcg tgacggttgc cgaaggcacg ccgctcgccg aggcgaaggc gctgatgcac agccaccgcc tcgagcgcgt gctcgtcgtc aacgatgcgt tcgagctgcg cggcctgatg acggtcaagg acatcacgaa gcagaccgag catccggaag cgtgcaagga cgagcacggc aagctgcgcg tcggcgccgc ggtcggcgtg ggggcggaca acgaggagcg cgtcgagctg ctcgtgcagg cgggcgtcga cgtgatcgtc gtcgacaccg cgcacggcca cagcaagggc gtgctcgagc gcgtgcgctg ggtcaagcag aacttcccga aggtcgaggt gatcggcggc aacatcgcga ccgcggccgc cgcgaaggcg ctcgtcgaat acggcgcgga cgcggtgaag gtcggcatcg gcccgggctc gatctgcacg acgcggatcg tcgcgggcgt cggcgtgccg cagatcagcg cgatcgcgaa cgtgtcggac gcgttgcgcg gcaccggcgt gccgtgcatc gccgacggcg gcatccgctt ctcgggcgac gtgtcgaagg cgctcgccgc cggcgcgaac gcggtgatga tgggcagcat gttcgcgggc accgaagagg cgccgggcga cgtgttcctg taccagggcc gccagtacaa gtcgtatcgc ggcatgggct cggtcggcgc gatgaaggac ggcgcggcgg accgctactt ccaggacaac tccgcgaaca tcgacaagct cgtgccggaa ggcatcgaag gccgcgtcgc gtacaagggc tcggtcaacg cgatcatctt ccagctgatc ggcggcgtgc gggcgagcat gggctactgc ggctgcaaga cgatcgccga gatgcacgag aaggccgagt tcgtcgagat caccgcggcc ggcatgcgcg agtcgcacgt gcacgacgtg cagatcacga aggaagcccc caactatcac gtggactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for ButhA.00062.b.A1.PS01167

PURIFICATION DATe:	8/10/2011
CONCENTRATION:	34.24mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRLIQKALT FDDVLLVPAF SDVLPRDTSL KTQLTRHISL NMPLVSAAMD TVTEGRLAIA MAQQGGVGIV HKNLTPVEQA REVAKVKRFE SGVVRDPITV PPSMKVRDVI ALSRQHGISG FPVLEGPKLV GIVTNRDLRF ETRLDEPVKS IMTPRERLVT VAEGTPLAEA KALMHSHRLE RVLVVNDAFE LRGLMTVKDI TKQTEHPEAC KDEHGKLRVG AAVGVGADNE ERVELLVQAG VDVIVVDTAH GHSKGVLERV RWVKQNFPKV EVIGGNIATA AAAKALVEYG ADAVKVGIGP GSICTTRIVA GVGVPQISAI ANVSDALRGT GVPCIADGGI RFSGDVSKAL AAGANAVMMG SMFAGTEEAP GDVFLYQGRQ YKSYRGMGSV GAMKDGAADR YFQDNSANID KLVPEGIEGR VAYKGSVNAI IFQLIGGVRA SMGYCGCKTI AEMHEKAEFV EITAAGMRES HVHDVQITKE APNYHVD

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGTCTGATC CAAAAAGCAC TCACGTTCGA TGACGTGCTC CTCGTCCCGG CGTTCTCCGA CGTTCTGCCG CGCGACACCA GCCTCAAGAC CCAGCTGACC CGCCACATCT CCCTGAACAT GCCGCTCGTG TCCGCCGCGA TGGACACGGT CACCGAAGGC CGCCTCGCGA TCGCGATGGC GCAGCAAGGC GGCGTCGGCA TCGTCCACAA GAATCTCACC CCCGTCGAGC AGGCGCGCGA GGTCGCGAAG GTCAAGCGCT TCGAATCGGG CGTCGTGCGC GATCCGATCA CCGTCCCGCC GAGCATGAAG GTGCGCGACG TGATCGCGCT GTCGCGCCAG CATGGCATCT CGGGTTTCCC GGTCCTCGAA GGCCCGAAGC TCGTCGGCAT CGTGACGAAC CGCGACCTGC GCTTCGAAAC CCGCCTCGAC GAGCCGGTCA AGTCGATCAT GACGCCGCGC GAGCGCCTCG TGACGGTTGC CGAAGGCACG CCGCTCGCCG AGGCGAAGGC GCTGATGCAC AGCCACCGCC TCGAGCGCGT GCTCGTCGTC AACGATGCGT TCGAGCTGCG CGGCCTGATG ACGGTCAAGG ACATCACGAA GCAGACCGAG CATCCGGAAG CGTGCAAGGA CGAGCACGGC AAGCTGCGCG TCGGCGCCGC GGTCGGCGTG GGGGCGGACA ACGAGGAGCG CGTCGAGCTG CTCGTGCAGG CGGGCGTCGA CGTGATCGTC GTCGACACCG CGCACGGCCA CAGCAAGGGC GTGCTCGAGC GCGTGCGCTG GGTCAAGCAG AACTTCCCGA AGGTCGAGGT GATCGGCGGC AACATCGCGA CCGCGGCCGC CGCGAAGGCG CTCGTCGAAT ACGGCGCGGA CGCGGTGAAG GTCGGCATCG GCCCGGGCTC GATCTGCACG ACGCGGATCG TCGCGGGCGT CGGCGTGCCG CAGATCAGCG CGATCGCGAA CGTGTCGGAC GCGTTGCGCG GCACCGGCGT GCCGTGCATC GCCGACGGCG GCATCCGCTT CTCGGGCGAC GTGTCGAAGG CGCTCGCCGC CGGCGCGAAC GCGGTGATGA TGGGCAGCAT GTTCGCGGGC ACCGAAGAGG CGCCGGGCGA CGTGTTCCTG TACCAGGGCC GCCAGTACAA GTCGTATCGC GGCATGGGCT CGGTCGGCGC GATGAAGGAC GGCGCGGCGG ACCGCTACTT CCAGGACAAC TCCGCGAACA TCGACAAGCT CGTGCCGGAA GGCATCGAAG GCCGCGTCGC GTACAAGGGC TCGGTCAACG CGATCATCTT CCAGCTGATC GGCGGCGTGC GGGCGAGCAT GGGCTACTGC GGCTGCAAGA CGATCGCCGA GATGCACGAG AAGGCCGAGT TCGTCGAGAT CACCGCGGCC GGCATGCGCG AGTCGCACGT GCACGACGTG CAGATCACGA AGGAAGCCCC CAACTATCAC GTGGACTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA