ButhA.00061.a

UDP-N-acetylglucosamine 2-epimerase

CENTER ID: ButhA.00061.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00061.a.B1.GE36049 Full length( ButhA.00061.a ) 1 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.384
RefSeq: YP_438540.1
UniProt: Q2T8F9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAYCDGPGL PPRDSRRESP RMRRPGRRNT VAHRAANRAA RPARYAHALV RCGMRPPRRC RIDRNNRESG TKMKKILLAF GTRPEAIKMA PLVRTLQTRA DVDARVCVTA QHRQMLDQVL SLFGIAPAYD LNVMRQSQTL ADVTTGILQT IGAVFDDFEP DLVLVHGDTT TTLAVSLAAF YRYLPIGHVE AGLRSGDIWS PWPEELNRRV TDAVSSWHFA PTERAQHNLF SEGVPTDGVV LTGNTVIDAL HDVKRMIEAD APLAREIAAR FPFLGRDERV VLITGHRRES FGEPFAHFCD ALRTLALRYP DVRFVYPLHL NPNVQEPAHS LLDGLPNIHL IAPQEYLSFV FLMSRARFII TDSGGIQEEG PALGKPVLVT RDTTERPEAI QAGTARLVGT NTERIVSEAS RLLDDDDAYD EMSHATNPYG DGHASERIVH ALMSKPHASD TARFPLAAIE APCNALALGL RALRSA
NT Sequence
GTGACCGCCT ACTGCGACGG CCCCGGCCTG CCGCCGCGCG ATTCGCGCCG CGAATCCCCG CGCATGCGCC GGCCCGGACG CCGCAATACC GTCGCACATC GTGCGGCGAA CCGCGCGGCG CGGCCCGCAC GCTACGCGCA CGCACTCGTG CGTTGCGGCA TGCGGCCGCC GCGCCGGTGC CGGATCGATC GGAACAACCG TGAATCGGGG ACCAAGATGA AGAAAATCCT GCTTGCGTTC GGCACTCGTC CCGAGGCCAT CAAGATGGCG CCGCTCGTGC GCACGCTGCA AACGCGAGCC GATGTCGACG CGCGGGTGTG CGTGACCGCC CAGCATCGGC AGATGCTCGA CCAGGTGCTT TCGCTGTTCG GCATCGCGCC CGCATACGAT CTGAACGTGA TGCGGCAAAG CCAGACGCTC GCCGACGTGA CGACGGGCAT CCTTCAGACG ATCGGCGCCG TGTTCGACGA TTTCGAGCCG GACCTCGTGC TCGTGCACGG CGACACGACG ACGACGCTCG CGGTGAGCCT CGCCGCGTTC TACCGTTACT TGCCGATCGG GCATGTCGAA GCGGGGCTGC GCAGCGGCGA CATCTGGTCG CCGTGGCCCG AGGAACTCAA CCGGCGCGTG ACGGACGCCG TGTCGTCGTG GCACTTCGCG CCGACCGAGC GCGCGCAGCA CAACCTGTTC AGCGAAGGCG TGCCGACGGA TGGCGTCGTG CTGACCGGCA ACACGGTGAT CGACGCGCTG CACGACGTGA AGCGCATGAT CGAAGCCGAC GCGCCGCTCG CGCGCGAAAT CGCCGCGCGG TTTCCGTTTC TCGGCCGCGA CGAGCGCGTC GTGCTGATCA CCGGCCATCG GCGCGAGAGT TTCGGCGAGC CGTTCGCGCA TTTCTGCGAC GCGCTGCGCA CGCTCGCGCT CCGCTATCCG GATGTGCGCT TCGTCTATCC GCTGCATCTG AACCCGAACG TGCAAGAGCC CGCGCACTCG CTGCTCGACG GCCTGCCGAA CATCCATCTG ATCGCGCCGC AGGAATATCT GTCGTTCGTC TTCCTGATGT CGCGCGCGCG TTTCATCATC ACCGATTCGG GCGGCATTCA GGAAGAAGGG CCCGCGCTCG GCAAGCCGGT GCTCGTCACG CGAGACACGA CCGAGCGGCC GGAGGCGATC CAGGCGGGCA CCGCGAGGCT CGTCGGCACG AACACCGAGC GCATCGTCAG CGAGGCGTCC CGGCTGCTCG ACGACGACGA CGCATACGAT GAGATGTCGC ACGCGACGAA TCCGTATGGC GACGGCCACG CGAGCGAGCG GATCGTCCAT GCGCTGATGA GCAAGCCGCA TGCGAGCGAC ACGGCGCGCT TCCCGCTCGC CGCGATCGAA GCGCCGTGCA ACGCGCTCGC GCTCGGCCTG CGTGCGCTGA GGTCCGCA
Details for ButhA.00061.a.B1.GE36049
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 53kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 86
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHHM VTAYCDGPGL PPRDSRRESP RMRRPGRRNT VAHRAANRAA RPARYAHALV RCGMRPPRRC RIDRNNRESG TKMKKILLAF GTRPEAIKMA PLVRTLQTRA DVDARVCVTA QHRQMLDQVL SLFGIAPAYD LNVMRQSQTL ADVTTGILQT IGAVFDDFEP DLVLVHGDTT TTLAVSLAAF YRYLPIGHVE AGLRSGDIWS PWPEELNRRV TDAVSSWHFA PTERAQHNLF SEXVPTDGVV LTGNTVIDAL HDVKRMIEAD APLAREIAAR FPFLGRDERV VLITGHRRES FGEPFAHFCD ALXTLALRYX XVXFXXXLHL XXXXXXXXSL XXXXXXSDXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcacca ccaccaccac caccatatgg tgaccgccta ctgcgacggc cccggcctgc cgccgcgcga ttcgcgccgc gaatccccgc gcatgcgccg gcccggacgc cgcaataccg tcgcacatcg tgcggcgaac cgcgcggcgc ggcccgcacg ctacgcgcac gcactcgtgc gttgcggcat gcggccgccg cgccggtgcc ggatcgatcg gaacaaccgt gaatcgggga ccaagatgaa gaaaatcctg cttgcgttcg gcactcgtcc cgaggccatc aagatggcgc cgctcgtgcg cacgctgcaa acgcgagccg atgtcgacgc gcgggtgtgc gtgaccgccc agcatcggca gatgctcgac caggtgcttt cgctgttcgg catcgcgccc gcatacgatc tgaacgtgat gcggcaaagc cagacgctcg ccgacgtgac gacgggcatc cttcagacga tcggcgccgt gttcgacgat ttcgagccgg acctcgtgct cgtgcacggc gacacgacga cgacgctcgc ggtgagcctc gccgcgttct accgttactt gccgatcggg catgtcgaag cggggctgcg cagcggcgac atctggtcgc cgtggcccga ggaactcaac cggcgcgtga cggacgccgt gtcgtcgtgg cacttcgcgc cgaccgagcg cgcgcagcac aacctgttca gcgaangcgt gccgacngat ggcgtcgtgc tgaccggcaa cacggtgatc gacgcgctgc acgacgtgaa gcgcatgatc gaagccgacg cgccgctcgc gcgcgaaatc gccgcgcggt ttccgtttct cggccgcgac gagcgcgtcg tgctgatcac cggccatcgg cgcgagagtt tcggcgagcc nttcgcgcat ttctgcgacg cgctgcncac gctcgcgctn cgctatncnn atgtgnnctt cgnnnatnnn ctncatctga nnnngaannn nnaancnnnn nantcnctnc ncgannnnnn nnnnncntct gatnnnnnnn nngnnnnnnn nnntnnnnnn nnannnnnnn nnnnnncnnt nntcntnnnn gnnnnnnnnn nnnnnnnnnn g
Expected Protein Sequence
MAHHHHHHMV TAYCDGPGLP PRDSRRESPR MRRPGRRNTV AHRAANRAAR PARYAHALVR CGMRPPRRCR IDRNNRESGT KMKKILLAFG TRPEAIKMAP LVRTLQTRAD VDARVCVTAQ HRQMLDQVLS LFGIAPAYDL NVMRQSQTLA DVTTGILQTI GAVFDDFEPD LVLVHGDTTT TLAVSLAAFY RYLPIGHVEA GLRSGDIWSP WPEELNRRVT DAVSSWHFAP TERAQHNLFS EGVPTDGVVL TGNTVIDALH DVKRMIEADA PLAREIAARF PFLGRDERVV LITGHRRESF GEPFAHFCDA LRTLALRYPD VRFVYPLHLN PNVQEPAHSL LDGLPNIHLI APQEYLSFVF LMSRARFIIT DSGGIQEEGP ALGKPVLVTR DTTERPEAIQ AGTARLVGTN TERIVSEASR LLDDDDAYDE MSHATNPYGD GHASERIVHA LMSKPHASDT ARFPLAAIEA PCNALALGLR ALRSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga ccgcctactg cgacggcccc ggcctgccgc cgcgcgattc gcgccgcgaa tccccgcgca tgcgccggcc cggacgccgc aataccgtcg cacatcgtgc ggcgaaccgc gcggcgcggc ccgcacgcta cgcgcacgca ctcgtgcgtt gcggcatgcg gccgccgcgc cggtgccgga tcgatcggaa caaccgtgaa tcggggacca agatgaagaa aatcctgctt gcgttcggca ctcgtcccga ggccatcaag atggcgccgc tcgtgcgcac gctgcaaacg cgagccgatg tcgacgcgcg ggtgtgcgtg accgcccagc atcggcagat gctcgaccag gtgctttcgc tgttcggcat cgcgcccgca tacgatctga acgtgatgcg gcaaagccag acgctcgccg acgtgacgac gggcatcctt cagacgatcg gcgccgtgtt cgacgatttc gagccggacc tcgtgctcgt gcacggcgac acgacgacga cgctcgcggt gagcctcgcc gcgttctacc gttacttgcc gatcgggcat gtcgaagcgg ggctgcgcag cggcgacatc tggtcgccgt ggcccgagga actcaaccgg cgcgtgacgg acgccgtgtc gtcgtggcac ttcgcgccga ccgagcgcgc gcagcacaac ctgttcagcg aaggcgtgcc gacggatggc gtcgtgctga ccggcaacac ggtgatcgac gcgctgcacg acgtgaagcg catgatcgaa gccgacgcgc cgctcgcgcg cgaaatcgcc gcgcggtttc cgtttctcgg ccgcgacgag cgcgtcgtgc tgatcaccgg ccatcggcgc gagagtttcg gcgagccgtt cgcgcatttc tgcgacgcgc tgcgcacgct cgcgctccgc tatccggatg tgcgcttcgt ctatccgctg catctgaacc cgaacgtgca agagcccgcg cactcgctgc tcgacggcct gccgaacatc catctgatcg cgccgcagga atatctgtcg ttcgtcttcc tgatgtcgcg cgcgcgtttc atcatcaccg attcgggcgg cattcaggaa gaagggcccg cgctcggcaa gccggtgctc gtcacgcgag acacgaccga gcggccggag gcgatccagg cgggcaccgc gaggctcgtc ggcacgaaca ccgagcgcat cgtcagcgag gcgtcccggc tgctcgacga cgacgacgca tacgatgaga tgtcgcacgc gacgaatccg tatggcgacg gccacgcgag cgagcggatc gtccatgcgc tgatgagcaa gccgcatgcg agcgacacgg cgcgcttccc gctcgccgcg atcgaagcgc cgtgcaacgc gctcgcgctc ggcctgcgtg cgctgaggtc cgcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat