SSGCID: Target Page

ButhA.00057.n

ABC transporter, ATP-binding protein (BTH_I0487)

CENTER ID:	ButhA.00057.n
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.n.A1.GE32710	full length		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132803
PATRIC ID:	fig\|271848.6.peg.3167
RefSeq:	YP_441044.1
UniProt:	Q2T1A5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNALPNRQPA GTSSSLVVRN LKKRYGSRTV VKDVSLDVKS GEVVGLLGPN GAGKTTSFYM IVGLVPLDAG DISLNGSPIS LMPIHKRASL GLSYLPQEAS VFRKLTVEQN VRAVLELQHD DNGKRLAKSE IGARTEALLE ELQIAHLREN PALSLSGGER RRVEIARALA SNPSFILLDE PFAGVDPIAV LEIQKIVKFL KQRNIGVLIT DHNVRETLGI CDHAYIISDG SVLASGAPKE IIENESVRRV YLGEHFRM

NT Sequence

gtgaacgcgc tccccaatcg ccaaccggcc ggcacgtcga gctcgctcgt cgtgcgcaac ctgaagaagc gctatggctc gcgcaccgtc gtcaaggacg tgtcgctcga cgtgaaaagc ggcgaggtcg tcggcctgct cggtccgaac ggcgcgggca agacgacgtc gttctacatg atcgtcggcc tcgtgccgct cgacgcgggc gacatctcgc tgaacggcag cccgatcagc ctgatgccga tccacaagcg cgcgtcgctc ggcctgtcgt acctgccgca ggaagcgtcg gtgttccgca agctgaccgt cgagcagaat gtgcgcgcgg tgctcgagct gcagcacgac gacaacggca agcggctggc gaagagcgag atcggcgcgc gcacggaggc gctcctcgag gagctgcaga tcgcgcacct gcgcgagaac ccggcgctgt cgctgtcggg cggcgaacgc cgccgcgtcg agatcgcgcg cgcgctcgcg agcaacccga gcttcatcct gctcgacgag ccgttcgcgg gcgtcgatcc gatcgcggtg ctcgaaatcc agaagatcgt gaaattcctg aagcagcgca acatcggcgt gctgatcacc gatcacaacg tgcgcgagac gctcggcatc tgcgatcacg cttacatcat cagcgacggc tcggtgctcg catccggcgc gccgaaggaa atcatcgaaa acgaaagcgt gcggcgcgtc tacctcggcg agcacttccg catgtag

Details for ButhA.00057.n.A1.GE32710

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNALPNRQ PAGTSSSLVV RNLKKRYGSR TVVKDVSLDV KSGEVVGLLG PNGAGKTTSF YMIVGLVPLD AGDISLNGSP ISLMPIHKRA SLGLSYLPQE ASVFRKLTVE QNVRAVLELQ HDDNGKRLAK SEIGARTEAL LEELQIAHLR ENPALSLSGG ERRRVEIARA LASNPSFILL DEPFAGVDPI AVLEIQKIVK FLKQRNIGVL ITDHNVRETL GICDHAYIIS DGSVLASGAP KEIIENESVR RVYLGEHFRM

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga acgcgctccc caatcgccaa ccggccggca cgtcgagctc gctcgtcgtg cgcaacctga agaagcgcta tggctcgcgc accgtcgtca aggacgtgtc gctcgacgtg aaaagcggcg aggtcgtcgg cctgctcggt ccgaacggcg cgggcaagac gacgtcgttc tacatgatcg tcggcctcgt gccgctcgac gcgggcgaca tctcgctgaa cggcagcccg atcagcctga tgccgatcca caagcgcgcg tcgctcggcc tgtcgtacct gccgcaggaa gcgtcggtgt tccgcaagct gaccgtcgag cagaatgtgc gcgcggtgct cgagctgcag cacgacgaca acggcaagcg gctggcgaag agcgagatcg gcgcgcgcac ggaggcgctc ctcgaggagc tgcagatcgc gcacctgcgc gagaacccgg cgctgtcgct gtcgggcggc gaacgccgcc gcgtcgagat cgcgcgcgcg ctcgcgagca acccgagctt catcctgctc gacgagccgt tcgcgggcgt cgatccgatc gcggtgctcg aaatccagaa gatcgtgaaa ttcctgaagc agcgcaacat cggcgtgctg atcaccgatc acaacgtgcg cgagacgctc ggcatctgcg atcacgctta catcatcagc gacggctcgg tgctcgcatc cggcgcgccg aaggaaatca tcgaaaacga aagcgtgcgg cgcgtctacc tcggcgagca cttccgcatg taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacgcg ctccccaatc gccaaccggc cggcacgtcg agctcgctcg tcgtgcgcaa cctgaagaag cgctatggct cgcgcaccgt cgtcaaggac gtgtcgctcg acgtgaaaag cggcgaggtc gtcggcctgc tcggtccgaa cggcgcgggc aagacgacgt cgttctacat gatcgtcggc ctcgtgccgc tcgacgcggg cgacatctcg ctgaacggca gcccgatcag cctgatgccg atccacaagc gcgcgtcgct cggcctgtcg tacctgccgc aggaagcgtc ggtgttccgc aagctgaccg tcgagcagaa tgtgcgcgcg gtgctcgagc tgcagcacga cgacaacggc aagcggctgg cgaagagcga gatcggcgcg cgcacggagg cgctcctcga ggagctgcag atcgcgcacc tgcgcgagaa cccggcgctg tcgctgtcgg gcggcgaacg ccgccgcgtc gagatcgcgc gcgcgctcgc gagcaacccg agcttcatcc tgctcgacga gccgttcgcg ggcgtcgatc cgatcgcggt gctcgaaatc cagaagatcg tgaaattcct gaagcagcgc aacatcggcg tgctgatcac cgatcacaac gtgcgcgaga cgctcggcat ctgcgatcac gcttacatca tcagcgacgg ctcggtgctc gcatccggcg cgccgaagga aatcatcgaa aacgaaagcg tgcggcgcgt ctacctcggc gagcacttcc gcatgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa