ButhA.00057.m

Glycerol-3-phosphate ABC transporter, ATP-binding protein

CENTER ID: ButhA.00057.m
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00057.m.A1.GE32709 Full length( ButhA.00057.m ) 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1303
RefSeq: YP_439372.1
UniProt: Q2T627

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARIVCTGLT KRYDGGAPVL HPLDLEIGDG EFIVLLGPSG CGKSTMLRMI AGLETITGGE LAIGGAVVND LAARERNVAM VFQNYALYPH MSVYENIAFG LRRLKVPAAE IDRRVREVAR VLSLDALLER KPRAMSGGQQ QRAAIARAMI KTPDVFLFDE PLSNLDAKLR AQLRGDIKRL HQRLKTTTLY VTHDQLEAMT LADRVVLMRG GRIEQLGTPA ELYSCPHTVF AAGFIGTPAM NFADGAIERT AGGVALAAGG ARWPLAARRF ASLADGRRVK LAIRPNYLRI ATGAGAAAGA RNAPAASGPG DRPSALALEG RVELVELLGA EALVTFDWNG APFAALVSAP AAPALGAVVT FTFDERDLHL FDAATGRNVM LPEAGAPASA APPGAAMRPP PDAPDAPDAP HGLAMSRS
NT Sequence
ATGGCACGGA TCGTCTGCAC CGGCTTGACC AAACGTTACG ACGGCGGCGC GCCCGTCCTG CATCCGCTCG ATCTCGAGAT CGGCGACGGC GAGTTCATCG TGCTGCTCGG CCCGTCGGGC TGCGGCAAAT CGACGATGCT GCGGATGATC GCCGGGCTCG AAACCATCAC GGGCGGCGAG CTCGCGATCG GCGGCGCCGT CGTCAACGAT CTGGCCGCAC GCGAGCGCAA TGTCGCGATG GTGTTCCAGA ACTACGCGCT GTATCCGCAT ATGAGCGTGT ACGAGAACAT CGCGTTCGGG CTGCGGCGCC TGAAGGTGCC CGCCGCCGAG ATCGACCGGC GCGTGCGCGA GGTCGCGCGC GTGCTGAGCC TCGACGCGCT CCTCGAGCGC AAGCCGCGCG CGATGTCGGG CGGCCAGCAG CAGCGCGCGG CGATCGCGCG CGCGATGATC AAGACGCCCG ACGTGTTCCT GTTCGACGAG CCGCTGTCGA ATCTCGACGC GAAGCTGCGC GCGCAGTTGC GCGGCGACAT CAAGCGGCTC CACCAGCGGC TGAAGACGAC GACGCTGTAC GTGACGCACG ATCAGCTCGA GGCGATGACG CTTGCCGACC GCGTCGTGCT GATGCGCGGC GGGCGCATCG AGCAGCTCGG CACGCCGGCC GAGCTGTACA GCTGCCCGCA CACCGTGTTC GCGGCGGGCT TCATCGGCAC GCCGGCGATG AACTTCGCCG ACGGCGCGAT CGAGCGCACG GCGGGCGGCG TCGCGCTCGC GGCGGGCGGC GCGCGCTGGC CGCTCGCGGC GCGCCGCTTC GCGAGCCTTG CCGACGGCCG GCGCGTGAAG CTCGCAATCC GGCCAAACTA TCTGCGGATC GCGACGGGCG CGGGCGCAGC CGCGGGCGCG CGGAACGCAC CTGCGGCGTC GGGGCCGGGT GACAGGCCAA GCGCGCTCGC GCTCGAAGGC CGCGTCGAGC TCGTCGAGCT GCTCGGCGCC GAGGCACTCG TCACGTTCGA CTGGAACGGC GCGCCGTTCG CGGCGCTCGT GAGCGCGCCG GCCGCCCCTG CGCTCGGCGC CGTGGTAACG TTCACGTTCG ACGAGCGCGA CCTGCATCTG TTCGACGCGG CCACCGGGCG CAACGTGATG CTGCCCGAGG CCGGCGCGCC CGCCAGCGCC GCACCGCCCG GCGCCGCGAT GCGGCCGCCG CCGGACGCGC CGGACGCGCC GGACGCGCCG CATGGGCTCG CGATGTCGCG CTCG
Details for ButhA.00057.m.A1.GE32709
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 61
Validated AA Sequence
MGTLEAQTQG PGSMARIVCT GLTKRYDGGA PVLHPLDLEI GDGEFIVLLG PSGCGKSTML RMIAGLETIT GGELAIGGAV VNDLAARERN VAMVFQNYAL YPHMSVYENI AFGLRRLKVP AAEIDRRVRE VARVLSLDAL LERKPRAMSG GQQQRAAIAR AMIKTPDVFL FDEPLSNLDA KLRAQLRGDI KRLHQRLKTT TLYVTHDQLE AMTLADRVVL MRGGRIEQLG TPAELYSCPH TXXAAGFIGT PAMNFAXGAI ERTXGGVALA AG
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcacg gatcgtctgc accggcttga ccaaacgtta cgacggcggc gcgcccgtcc tgcatccgct cgatctcgag atcggcgacg gcgagttcat cgtgctgctc ggcccgtcgg gctgcggcaa atcgacgatg ctgcggatga tcgccgggct cgaaaccatc acgggcggcg agctcgcgat cggcggcgcc gtcgtcaacg atctggccgc acgcgagcgc aatgtcgcga tggtgttcca gaactacgcg ctgtatccgc atatgagcgt gtacgagaac atcgcgttcg ggctgcggcg cctgaaggtg cccgccgccg agatcgaccg gcgcgtgcgc gaggtcgcgc gcgtgctgag cctcgacgcg ctcctcgagc gcaagccgcg cgcgatgtcg ggcggccagc agcagcgcgc ggcgatcgcg cgcgcgatga tcaagacgcc cgacgtgttc ctgttcgacg agccgctgtc gaatctcgac gcgaagctgc gcgcgcagtt gcgcggcgac atcaagcggc tccaccagcg gctgaagacg acgacgctgt acgtgacgca cgatcagctc gaggcgatga cgcttgccga ccgcgtcgtg ctgatgcgcg gcgggcgcat cgagcagctc ggcacgccgg ccgagctgta cagctgcccg cacaccgngn tcgcggcggg cttcatcggc acgccggcga tgaacttcgc cnanggngcg atcgagcgca cggnnggcgg cgtcgcgctc gcggcgggcg gc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARIVCTGL TKRYDGGAPV LHPLDLEIGD GEFIVLLGPS GCGKSTMLRM IAGLETITGG ELAIGGAVVN DLAARERNVA MVFQNYALYP HMSVYENIAF GLRRLKVPAA EIDRRVREVA RVLSLDALLE RKPRAMSGGQ QQRAAIARAM IKTPDVFLFD EPLSNLDAKL RAQLRGDIKR LHQRLKTTTL YVTHDQLEAM TLADRVVLMR GGRIEQLGTP AELYSCPHTV FAAGFIGTPA MNFADGAIER TAGGVALAAG GARWPLAARR FASLADGRRV KLAIRPNYLR IATGAGAAAG ARNAPAASGP GDRPSALALE GRVELVELLG AEALVTFDWN GAPFAALVSA PAAPALGAVV TFTFDERDLH LFDAATGRNV MLPEAGAPAS AAPPGAAMRP PPDAPDAPDA PHGLAMSRS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacggatc gtctgcaccg gcttgaccaa acgttacgac ggcggcgcgc ccgtcctgca tccgctcgat ctcgagatcg gcgacggcga gttcatcgtg ctgctcggcc cgtcgggctg cggcaaatcg acgatgctgc ggatgatcgc cgggctcgaa accatcacgg gcggcgagct cgcgatcggc ggcgccgtcg tcaacgatct ggccgcacgc gagcgcaatg tcgcgatggt gttccagaac tacgcgctgt atccgcatat gagcgtgtac gagaacatcg cgttcgggct gcggcgcctg aaggtgcccg ccgccgagat cgaccggcgc gtgcgcgagg tcgcgcgcgt gctgagcctc gacgcgctcc tcgagcgcaa gccgcgcgcg atgtcgggcg gccagcagca gcgcgcggcg atcgcgcgcg cgatgatcaa gacgcccgac gtgttcctgt tcgacgagcc gctgtcgaat ctcgacgcga agctgcgcgc gcagttgcgc ggcgacatca agcggctcca ccagcggctg aagacgacga cgctgtacgt gacgcacgat cagctcgagg cgatgacgct tgccgaccgc gtcgtgctga tgcgcggcgg gcgcatcgag cagctcggca cgccggccga gctgtacagc tgcccgcaca ccgtgttcgc ggcgggcttc atcggcacgc cggcgatgaa cttcgccgac ggcgcgatcg agcgcacggc gggcggcgtc gcgctcgcgg cgggcggcgc gcgctggccg ctcgcggcgc gccgcttcgc gagccttgcc gacggccggc gcgtgaagct cgcaatccgg ccaaactatc tgcggatcgc gacgggcgcg ggcgcagccg cgggcgcgcg gaacgcacct gcggcgtcgg ggccgggtga caggccaagc gcgctcgcgc tcgaaggccg cgtcgagctc gtcgagctgc tcggcgccga ggcactcgtc acgttcgact ggaacggcgc gccgttcgcg gcgctcgtga gcgcgccggc cgcccctgcg ctcggcgccg tggtaacgtt cacgttcgac gagcgcgacc tgcatctgtt cgacgcggcc accgggcgca acgtgatgct gcccgaggcc ggcgcgcccg ccagcgccgc accgcccggc gccgcgatgc ggccgccgcc ggacgcgccg gacgcgccgg acgcgccgca tgggctcgcg atgtcgcgct cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa