SSGCID: Target Page

ButhA.00057.l

ABC transporter domain protein

CENTER ID:	ButhA.00057.l
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.l.A1.GE32708	Full length( ButhA.00057.l )		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2046
RefSeq:	YP_440029.1
UniProt:	Q2T470

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKLERVHTH YGAIEALSGV SIEVKKGEIV TLIGSNGAGK TTLMMTVCGT PRASSGRVLF EGDDITAMPT HEIMRQGLAI SPEGRRVFPS LTVLENLKMG GFFASRHEID EGVEHVYRLF PRLKERSAQR AGTMSGGEQQ MLAIGRALMS RPRLLLLDEP TLGLAPLVIA QIFDIIRTIR DEGVTVFLVE QNAHKALQVA DRGYVLETGR VVLADTGANL LENDRIRAAY LGG

NT Sequence

ATGCTGAAGC TGGAGAGGGT CCACACGCAT TACGGCGCGA TCGAGGCGCT GTCGGGCGTG TCGATCGAGG TGAAGAAGGG CGAGATCGTC ACGCTGATCG GCAGCAACGG CGCGGGCAAG ACGACGCTGA TGATGACCGT GTGCGGCACG CCGCGCGCCT CGAGCGGGCG CGTGCTGTTC GAAGGCGACG ACATCACCGC GATGCCGACG CACGAAATCA TGCGCCAGGG GCTCGCGATC TCGCCGGAAG GGCGCCGCGT GTTTCCGAGC CTGACCGTGC TCGAGAACCT GAAGATGGGC GGCTTCTTTG CCAGCCGCCA CGAGATCGAC GAAGGCGTCG AGCATGTGTA TCGGCTGTTT CCACGGCTCA AGGAGCGCTC CGCGCAGCGC GCGGGCACGA TGTCGGGCGG CGAGCAGCAG ATGCTCGCGA TCGGCCGCGC GCTGATGAGC CGGCCGCGCC TGCTGCTGCT CGACGAGCCG ACGCTCGGCT TGGCGCCGCT CGTGATCGCG CAGATCTTCG ACATCATCCG CACGATTCGC GACGAGGGCG TGACGGTGTT TCTCGTCGAG CAGAACGCTC ACAAGGCGCT GCAGGTTGCC GATCGCGGTT ACGTGCTCGA AACCGGGCGC GTCGTGCTCG CCGACACCGG CGCGAACCTG CTCGAGAACG ACCGGATCAG GGCCGCGTAT CTCGGCGGA

Details for ButhA.00057.l.A1.GE32708

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKLERVHT HYGAIEALSG VSIEVKKGEI VTLIGSNGAG KTTLMMTVCG TPRASSGRVL FEGDDITAMP THEIMRQGLA ISPEGRRVFP SLTVLENLKM GGFFASRHEI DEGVEHVYRL FPRLKERSAQ RAGTMSGGEQ QMLAIGRALM SRPRLLLLDE PTLGLAPLVI AQIFDIIRTI RDEGVTVFLV EQNAHKALQV ADRGYVLETG RVVLADTGAN LLENDRIXAA YLGG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga agctggagag ggtccacacg cattacggcg cgatcgaggc gctgtcgggc gtgtcgatcg aggtgaagaa gggcgagatc gtcacgctga tcggcagcaa cggcgcgggc aagacgacgc tgatgatgac cgtgtgcggc acgccgcgcg cctcgagcgg gcgcgtgctg ttcgaaggcg acgacatcac cgcgatgccg acgcacgaaa tcatgcgcca ggggctcgcg atctcgccgg aagggcgccg cgtgtttccg agcctgaccg tgctcgagaa cctgaagatg ggcggcttct ttgccagccg ccacgagatc gacgaaggcg tcgagcatgt gtatcggctg tttccacggc tcaaggagcg ctccgcgcag cgcgcgggca cgatgtcggg cggcgagcag cagatgctcg cgatcggccg cgcgctgatg agccggccgc gcctgctgct gctcgacgag ccgacgctcg gcttggcgcc gctcgtgatc gcgcagatct tcgacatcat ccgcacgatt cgcgacgagg gcgtgacggt gtttctcgtc gagcagaacg ctcacaaggc gctgcaggtt gccgatcgcg gttacgtgct cgaaaccggg cgcgtcgtgc tcgccgacac cggcgcgaac ctgctcgaga acgaccggat cnnggccgcg tatctcggcg gataataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagctg gagagggtcc acacgcatta cggcgcgatc gaggcgctgt cgggcgtgtc gatcgaggtg aagaagggcg agatcgtcac gctgatcggc agcaacggcg cgggcaagac gacgctgatg atgaccgtgt gcggcacgcc gcgcgcctcg agcgggcgcg tgctgttcga aggcgacgac atcaccgcga tgccgacgca cgaaatcatg cgccaggggc tcgcgatctc gccggaaggg cgccgcgtgt ttccgagcct gaccgtgctc gagaacctga agatgggcgg cttctttgcc agccgccacg agatcgacga aggcgtcgag catgtgtatc ggctgtttcc acggctcaag gagcgctccg cgcagcgcgc gggcacgatg tcgggcggcg agcagcagat gctcgcgatc ggccgcgcgc tgatgagccg gccgcgcctg ctgctgctcg acgagccgac gctcggcttg gcgccgctcg tgatcgcgca gatcttcgac atcatccgca cgattcgcga cgagggcgtg acggtgtttc tcgtcgagca gaacgctcac aaggcgctgc aggttgccga tcgcggttac gtgctcgaaa ccgggcgcgt cgtgctcgcc gacaccggcg cgaacctgct cgagaacgac cggatcaggg ccgcgtatct cggcggataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa