SSGCID: Target Page

ButhA.00057.j

Branched-chain amino acid ABC transporter, ATP-binding protein, putative

CENTER ID:	ButhA.00057.j
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.j.A1.GE32706	Full length( ButhA.00057.j )		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2693
RefSeq:	YP_440605.1
UniProt:	Q2T2G8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLEVKDLVV HRANKPVLHG VSLAVAPGRV TALVGANGAG KSTLVMSIAG TLPATSGAVL LDGAPLGALR PEAVRRRGIA VVPEGHRVLG DLSVRDNLRA AGAFLSARRL NDAIDRALAI FPELEAKLDA RGNDLSGGQK QMVCVSQALI GEPHTLLIDE LSLGLAPAVT KRLAQTVARI ANDGVAVLLI EQFTTIALAL ATNAYVLERG RVAFAGTAQM LRERPEILHG SYLASKGGGA NAA

NT Sequence

ATGCGGCTCG AAGTGAAAGA CCTCGTCGTT CATCGCGCGA ACAAGCCCGT GCTGCACGGC GTGTCGCTCG CGGTCGCGCC CGGCCGGGTG ACGGCGCTCG TCGGCGCGAA CGGCGCCGGC AAATCGACGC TCGTGATGAG CATTGCCGGC ACGCTGCCCG CGACGTCGGG CGCCGTGCTG CTCGACGGCG CGCCGCTCGG CGCGCTGCGC CCCGAGGCGG TGCGCCGCCG CGGCATCGCG GTCGTGCCGG AAGGGCATCG CGTGCTCGGC GACCTGTCGG TGCGCGACAA CCTGCGCGCC GCGGGCGCGT TCCTGTCGGC GCGGCGGCTC AATGACGCGA TCGACCGCGC GCTCGCGATT TTCCCGGAAC TCGAGGCGAA GCTCGATGCG CGCGGCAACG ATCTGTCGGG CGGGCAGAAG CAGATGGTAT GCGTGTCGCA GGCGCTGATC GGCGAGCCGC ACACGCTGCT CATCGACGAG CTGTCGCTCG GCCTGGCGCC CGCGGTGACG AAGCGTCTCG CGCAGACCGT CGCGCGGATC GCGAACGACG GCGTCGCGGT GCTGCTGATC GAGCAGTTCA CGACGATCGC GCTCGCGCTC GCGACGAACG CGTATGTGCT CGAACGCGGG CGCGTAGCGT TCGCGGGCAC CGCGCAGATG CTGCGCGAGC GGCCGGAGAT CTTGCACGGC AGTTATCTCG CGTCGAAGGG CGGCGGCGCA AACGCGGCG

Details for ButhA.00057.j.A1.GE32706

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRLEVKDLV VHRANKPVLH GVSLAVAPGR VTALVGANGA GKSTLVMSIA GTLPATSGAV LLDGAPLGAL RPEAVRRRGI AVVPEGHRVL GDLSVRDNLR AAGAFLSARR LNDAIDRALA IFPELEAKLD ARGNDLSGGQ KQMVCVSQAL IGEPHTLLID ELSLGLAPAV TKRLAQTVAR IANDGVAVLL IEQFTTIALA LATNAYVLER GRVAXAGTAQ MLRERXEILH GSYLASKGGG ANAA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggc tcgaagtgaa agacctcgtc gttcatcgcg cgaacaagcc cgtgctgcac ggcgtgtcgc tcgcggtcgc gcccggccgg gtgacggcgc tcgtcggcgc gaacggcgcc ggcaaatcga cgctcgtgat gagcattgcc ggcacgctgc ccgcgacgtc gggcgccgtg ctgctcgacg gcgcgccgct cggcgcgctg cgccccgagg cggtgcgccg ccgcggcatc gcggtcgtgc cggaagggca tcgcgtgctc ggcgacctgt cggtgcgcga caacctgcgc gccgcgggcg cgttcctgtc ggcgcggcgg ctcaatgacg cgatcgaccg cgcgctcgcg attttcccgg aactcgaggc gaagctcgat gcgcgcggca acgatctgtc gggcgggcag aagcagatgg tatgcgtgtc gcaggcgctg atcggcgagc cgcacacgct gctcatcgac gagctgtcgc tcggcctggc gcccgcggtg acgaagcgtc tcgcgcagac cgtcgcgcgg atcgcgaacg acggcgtcgc ggtgctgctg atcgagcagt tcacgacgat cgcgctcgcg ctcgcgacga acgcgtatgt gctcgaacgc gggcgcgtag cgntcgcggg caccgcgcag atgctgcgcg agcgnncgga gatcttgcac ggcagttatc tcgcgtcgaa gggcggcggc gcaaacgcgg cgtaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggctcgaa gtgaaagacc tcgtcgttca tcgcgcgaac aagcccgtgc tgcacggcgt gtcgctcgcg gtcgcgcccg gccgggtgac ggcgctcgtc ggcgcgaacg gcgccggcaa atcgacgctc gtgatgagca ttgccggcac gctgcccgcg acgtcgggcg ccgtgctgct cgacggcgcg ccgctcggcg cgctgcgccc cgaggcggtg cgccgccgcg gcatcgcggt cgtgccggaa gggcatcgcg tgctcggcga cctgtcggtg cgcgacaacc tgcgcgccgc gggcgcgttc ctgtcggcgc ggcggctcaa tgacgcgatc gaccgcgcgc tcgcgatttt cccggaactc gaggcgaagc tcgatgcgcg cggcaacgat ctgtcgggcg ggcagaagca gatggtatgc gtgtcgcagg cgctgatcgg cgagccgcac acgctgctca tcgacgagct gtcgctcggc ctggcgcccg cggtgacgaa gcgtctcgcg cagaccgtcg cgcggatcgc gaacgacggc gtcgcggtgc tgctgatcga gcagttcacg acgatcgcgc tcgcgctcgc gacgaacgcg tatgtgctcg aacgcgggcg cgtagcgttc gcgggcaccg cgcagatgct gcgcgagcgg ccggagatct tgcacggcag ttatctcgcg tcgaagggcg gcggcgcaaa cgcggcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa