SSGCID: Target Page

ButhA.00057.g

Branched-chain amino acid ABC transporter, ATP-binding protein, putative

CENTER ID:	ButhA.00057.g
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.g.A1.GE32703	Full length( ButhA.00057.g )		1	230

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4282
RefSeq:	YP_442046.1
UniProt:	Q2SYF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTIESLNQY YGGSHILRDV SLTAETGRLT VLLGRNGVGK TTLLRCLMGV VPTKSGSIAW RGKPLGALPP HARVASGLAY VPQGRDIFAR LTVEENLLVG AASRKAPSKV PDRIYTLFPV LKDMRARRGG DLSGGQQQQL AIGRALMSEP QLLILDEPTE GIQPSIIQDI GRTLRQLVDE TKMTVLLVEQ YYDFARSIAD RYWVMSRGEI VAGGAGSEMD AHGVRELIAV

NT Sequence

ATGCTGACAA TCGAATCGCT GAACCAATAC TACGGCGGCA GCCACATCCT GCGCGACGTG AGCCTCACCG CCGAAACGGG CCGGCTCACC GTGCTGCTCG GGCGCAACGG CGTCGGCAAG ACGACGCTGC TGCGCTGCCT GATGGGCGTC GTGCCGACGA AAAGCGGCAG CATCGCGTGG CGCGGCAAGC CGCTCGGCGC GCTGCCTCCC CACGCGCGCG TCGCGTCGGG CCTCGCCTAT GTGCCGCAGG GCCGCGACAT CTTCGCGCGG CTCACCGTCG AGGAGAATCT GCTCGTCGGC GCGGCGAGCC GCAAGGCGCC GTCCAAAGTG CCCGACCGGA TCTACACGCT CTTTCCGGTG CTCAAGGACA TGCGCGCGCG ACGCGGCGGC GACCTGTCGG GCGGCCAGCA GCAGCAGCTC GCGATCGGCC GCGCGCTGAT GAGCGAGCCG CAATTGCTGA TCCTAGACGA GCCGACGGAA GGCATTCAGC CGTCGATCAT CCAGGACATC GGCCGCACGC TGCGGCAGCT CGTCGACGAA ACGAAGATGA CCGTGCTGCT CGTCGAGCAG TACTACGATT TCGCGCGTTC GATCGCCGAT CGCTACTGGG TGATGAGCCG CGGCGAGATC GTCGCGGGCG GCGCGGGCAG CGAGATGGAC GCGCACGGCG TGCGCGAGCT GATCGCGGTC

Details for ButhA.00057.g.A1.GE32703

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLTIESLNQ YYGGSHILRD VSLTAETGRL TVLLGRNGVG KTTLLRCLMG VVPTKSGSIA WRGKPLGALP PHARVASGLA YVPQGRDIFA RLTVEENLLV GAASRKAPSK VPDRIYTLFP VLKDMRARRG GDLSGGQQQQ LAIGRALMSE PQLLILDEPT EGIQPSIIQD IGRTLRQLVD ETKMTVLLVE QYYDFARSIA DRYWVMSRGE IVAGGAGSEM DAHGVRELIA V

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga caatcgaatc gctgaaccaa tactacggcg gcagccacat cctgcgcgac gtgagcctca ccgccgaaac gggccggctc accgtgctgc tcgggcgcaa cggcgtcggc aagacgacgc tgctgcgctg cctgatgggc gtcgtgccga cgaaaagcgg cagcatcgcg tggcgcggca agccgctcgg cgcgctgcct ccccacgcgc gcgtcgcgtc gggcctcgcc tatgtgccgc agggccgcga catcttcgcg cggctcaccg tcgaggagaa tctgctcgtc ggcgcggcga gccgcaaggc gccgtccaaa gtgcccgacc ggatctacac gctctttccg gtgctcaagg acatgcgcgc gcgacgcggc ggcgacctgt cgggcggcca gcagcagcag ctcgcgatcg gccgcgcgct gatgagcgag ccgcaattgc tgatcctaga cgagccgacg gaaggcattc agccgtcgat catccaggac atcggccgca cgctgcggca gctcgtcgac gaaacgaaga tgaccgtgct gctcgtcgag cagtactacg atttcgcgcg ttcgatcgcc gatcgctact gggtgatgag ccgcggcgag atcgtcgcgg gcggcgcggg cagcgagatg gacgcgcacg gcgtgcgcga gctgatcgcg gtctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgacaatc gaatcgctga accaatacta cggcggcagc cacatcctgc gcgacgtgag cctcaccgcc gaaacgggcc ggctcaccgt gctgctcggg cgcaacggcg tcggcaagac gacgctgctg cgctgcctga tgggcgtcgt gccgacgaaa agcggcagca tcgcgtggcg cggcaagccg ctcggcgcgc tgcctcccca cgcgcgcgtc gcgtcgggcc tcgcctatgt gccgcagggc cgcgacatct tcgcgcggct caccgtcgag gagaatctgc tcgtcggcgc ggcgagccgc aaggcgccgt ccaaagtgcc cgaccggatc tacacgctct ttccggtgct caaggacatg cgcgcgcgac gcggcggcga cctgtcgggc ggccagcagc agcagctcgc gatcggccgc gcgctgatga gcgagccgca attgctgatc ctagacgagc cgacggaagg cattcagccg tcgatcatcc aggacatcgg ccgcacgctg cggcagctcg tcgacgaaac gaagatgacc gtgctgctcg tcgagcagta ctacgatttc gcgcgttcga tcgccgatcg ctactgggtg atgagccgcg gcgagatcgt cgcgggcggc gcgggcagcg agatggacgc gcacggcgtg cgcgagctga tcgcggtcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a