SSGCID: Target Page

ButhA.00057.f

Branched-chain amino acid ABC transporter, ATP-binding protein

CENTER ID:	ButhA.00057.f
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.f.A1.GE32702	Full length( ButhA.00057.f )		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4283
RefSeq:	YP_442047.1
UniProt:	Q2SYF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNENVTIPKL ALPDGGDRLA VSGTAQMSHV VTPGAIDTSH GAILYLEDIE VSFDGFRALR KLSLAIDVGE LRCVIGPNGA GKTTMMDVIT GKTRPDSGHA FLGQTLDLAR MTEPQIARAG IGRKFQKPTV FEQHPVWENL ELAMAADKRW FASLRARLDR AAQAQIEETL ALIGLEDSAY RLAGELSHGQ KQRLEIGMLL MQRPALLLLD EPAAGMTDHE TMELAKLLNT LRGTCSMMVV EHDMEFVAAL AGDTGRVTVM AEGAVLAHGT LAQVKRDEAV IESYLGR

NT Sequence

ATGAACGAGA ACGTTACGAT TCCCAAGCTC GCGCTGCCGG ACGGCGGCGA TCGGCTCGCC GTCAGCGGCA CGGCGCAGAT GTCGCACGTC GTCACGCCTG GCGCGATCGA CACGTCGCAC GGCGCGATTC TCTATCTAGA GGACATCGAA GTGAGCTTCG ACGGCTTTCG CGCGCTCAGG AAGCTGTCGC TCGCGATCGA CGTCGGCGAG TTGCGCTGCG TGATCGGCCC GAACGGCGCC GGCAAGACGA CGATGATGGA CGTGATCACC GGCAAGACGC GGCCGGACTC GGGCCACGCG TTCCTCGGCC AGACGCTCGA CCTCGCACGG ATGACCGAGC CGCAGATCGC GCGCGCGGGC ATCGGCCGCA AGTTCCAGAA GCCGACGGTG TTCGAGCAGC ATCCGGTGTG GGAGAACCTC GAGCTCGCGA TGGCGGCCGA CAAGCGCTGG TTCGCGTCGC TGCGCGCGCG GCTCGATCGC GCGGCGCAGG CGCAAATCGA GGAGACGCTC GCGCTGATCG GGCTCGAGGA CAGCGCGTAC CGGCTCGCGG GCGAGCTGTC GCACGGGCAG AAGCAGCGGC TCGAGATCGG CATGCTGCTG ATGCAGCGCC CCGCGCTCTT GCTGCTCGAC GAGCCCGCAG CCGGCATGAC CGACCACGAG ACGATGGAGC TCGCCAAGCT GCTGAACACG CTGCGCGGCA CCTGCTCGAT GATGGTCGTC GAACACGACA TGGAGTTCGT CGCGGCGCTC GCAGGCGACA CGGGGCGCGT GACGGTGATG GCGGAAGGCG CGGTGCTCGC GCACGGCACG CTCGCTCAGG TGAAGCGCGA CGAAGCGGTG ATCGAGTCTT ACCTCGGACG A

Details for ButhA.00057.f.A1.GE32702

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	94

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNENVTIPK LALPDGGDRL AVSGTAQMSH VVTPGAIDTS HGAILYLEDI EVSFDGFRAL RKLSLAIDVG ELRCVIGPNG AGKTTMMDVI TGKTRPDSGH AFLGQTLDLA RMTEPQIARA GIGRKFQKPT VFEQHPVWEN LELAMAADKR WFASLRARLD RAAXAQIEET LALIGLEDSA YRLAGELSHG QKQRLEIGML LMQRPALLLL DEPAAGMTDH ETXELAKLLN TLRGTCSMMV VEHDMXFVAA LAXXTGRVTV XXXXXXSXTX XS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacg agaacgttac gattcccaag ctcgcgctgc cggacggcgg cgatcggctc gccgtcagcg gcacggcgca gatgtcgcac gtcgtcacgc ctggcgcgat cgacacgtcg cacggcgcga ttctctatct agaggacatc gaagtgagct tcgacggctt tcgcgcgctc aggaagctgt cgctcgcgat cgacgtcggc gagttgcgct gcgtgatcgg cccgaacggc gccggcaaga cgacgatgat ggacgtgatc accggcaaga cgcggccgga ctcgggccac gcgttcctcg gccagacgct cgacctcgca cggatgaccg agccgcagat cgcgcgcgcg ggcatcggcc gcaagttcca gaagccgacg gtgttcgagc agcatccggt gtgggagaac ctcgagctcg cgatggcggc cgacaagcgc tggttcgcgt cgctgcgcgc gcggctcgat cgcgcggcgc angcgcaaat cgaggagacg ctcgcgctga tcgggctcga ggacagcgcg taccggctcg cgggcgagct gtcgcacggg cagaagcagc ggctcgagat cggcatgctg ctgatgcagc gccccgcgct cttgctgctc gacgagcccg cagccggcat gaccgaccac gagacgatng agctcgccaa gctgctgaac acgctgcgcg gcacctgctc gatgatggtc gtcgaacacg acatggnntt cgtcgcggcg ctngcngnnn acacggggcg cgtgacggtg anngngnnnn nncngngntc gnncacngnn ngctcgct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNENVTIPK LALPDGGDRL AVSGTAQMSH VVTPGAIDTS HGAILYLEDI EVSFDGFRAL RKLSLAIDVG ELRCVIGPNG AGKTTMMDVI TGKTRPDSGH AFLGQTLDLA RMTEPQIARA GIGRKFQKPT VFEQHPVWEN LELAMAADKR WFASLRARLD RAAQAQIEET LALIGLEDSA YRLAGELSHG QKQRLEIGML LMQRPALLLL DEPAAGMTDH ETMELAKLLN TLRGTCSMMV VEHDMEFVAA LAGDTGRVTV MAEGAVLAHG TLAQVKRDEA VIESYLGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgagaac gttacgattc ccaagctcgc gctgccggac ggcggcgatc ggctcgccgt cagcggcacg gcgcagatgt cgcacgtcgt cacgcctggc gcgatcgaca cgtcgcacgg cgcgattctc tatctagagg acatcgaagt gagcttcgac ggctttcgcg cgctcaggaa gctgtcgctc gcgatcgacg tcggcgagtt gcgctgcgtg atcggcccga acggcgccgg caagacgacg atgatggacg tgatcaccgg caagacgcgg ccggactcgg gccacgcgtt cctcggccag acgctcgacc tcgcacggat gaccgagccg cagatcgcgc gcgcgggcat cggccgcaag ttccagaagc cgacggtgtt cgagcagcat ccggtgtggg agaacctcga gctcgcgatg gcggccgaca agcgctggtt cgcgtcgctg cgcgcgcggc tcgatcgcgc ggcgcaggcg caaatcgagg agacgctcgc gctgatcggg ctcgaggaca gcgcgtaccg gctcgcgggc gagctgtcgc acgggcagaa gcagcggctc gagatcggca tgctgctgat gcagcgcccc gcgctcttgc tgctcgacga gcccgcagcc ggcatgaccg accacgagac gatggagctc gccaagctgc tgaacacgct gcgcggcacc tgctcgatga tggtcgtcga acacgacatg gagttcgtcg cggcgctcgc aggcgacacg gggcgcgtga cggtgatggc ggaaggcgcg gtgctcgcgc acggcacgct cgctcaggtg aagcgcgacg aagcggtgat cgagtcttac ctcggacgat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa