ButhA.00057.e

Branched-chain amino acid ABC transporter, ATP-binding protein, putative

CENTER ID: ButhA.00057.e
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00057.e.A1.GE32701 Full length( ButhA.00057.e ) 1 234
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5510
RefSeq: YP_443166.1
UniProt: Q2SV83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSALVELRGV RAGYGALPVL DGVDLRIAAG EAVALVGRNG SGRSTLAKAI MGMVRVAGTV RVDGIDVAGA PTFAVARHRV GYVAESRDVF ALLSVEDNLR LGLRGVSGAA ARAALDAARE RYPMLAERWR TKAGVLSGGE QQMLALARAL IGAPRVLIAD EPAEGLAPLA VEQVRACLAG AKAQGVALLL IEQRMQLAPA LASRVAVMGR GRIVYDGPAK ALDAGIVERW LSVG
NT Sequence
ATGAGCGCGC TCGTCGAATT GCGCGGCGTG CGCGCCGGAT ACGGCGCGCT GCCGGTGCTC GACGGCGTCG ATCTGCGGAT CGCGGCGGGC GAGGCCGTCG CGCTCGTCGG GCGCAACGGC TCGGGCCGCT CGACGCTCGC GAAGGCGATC ATGGGAATGG TGCGGGTTGC GGGGACGGTG CGCGTCGACG GCATCGACGT CGCGGGCGCG CCGACGTTCG CGGTCGCGCG GCATCGGGTC GGCTACGTCG CCGAGAGCCG CGACGTGTTC GCGCTCCTGA GCGTCGAGGA CAATCTGCGG CTCGGCCTGC GCGGCGTGTC GGGCGCCGCC GCGCGCGCGG CGCTCGACGC CGCGCGCGAG CGCTACCCGA TGCTCGCCGA GCGCTGGCGA ACGAAGGCGG GCGTGCTGTC GGGCGGCGAG CAGCAGATGC TCGCGCTCGC GCGCGCGTTG ATCGGCGCGC CGCGCGTGCT GATCGCCGAC GAGCCGGCCG AAGGGCTCGC GCCGCTCGCG GTCGAGCAGG TTCGCGCGTG TCTCGCCGGC GCGAAGGCGC AAGGCGTCGC GCTGCTGCTG ATCGAGCAGC GGATGCAGCT CGCGCCGGCG CTCGCGTCGC GGGTCGCGGT GATGGGGCGG GGAAGGATCG TCTACGACGG CCCGGCGAAG GCGCTCGATG CGGGCATCGT CGAGCGTTGG CTCAGCGTCG GT
Details for ButhA.00057.e.A1.GE32701
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSALVELRG VRAGYGALPV LDGVDLRIAA GEAVALVGRN GSGRSTLAKA IMGMVRVAGT VRVDGIDVAG APTFAVARHR VGYVAESRDV FALLSVEDNL RLGLRGVSGA AARAALDAAR ERYPMLAERW RTKAGVLSGG EQQMLALARA LIGAPRVLIA DEPAEGLAPL AVEQVRACLA GAXAQGVALL LIEQRMQLAP ALASRVAVMG RGRIVYDGPA XXLDAGIVER WLSVX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg cgctcgtcga attgcgcggc gtgcgcgccg gatacggcgc gctgccggtg ctcgacggcg tcgatctgcg gatcgcggcg ggcgaggccg tcgcgctcgt cgggcgcaac ggctcgggcc gctcgacgct cgcgaaggcg atcatgggaa tggtgcgggt tgcggggacg gtgcgcgtcg acggcatcga cgtcgcgggc gcgccgacgt tcgcggtcgc gcggcatcgg gtcggctacg tcgccgagag ccgcgacgtg ttcgcgctcc tgagcgtcga ggacaatctg cggctcggcc tgcgcggcgt gtcgggcgcc gccgcgcgcg cggcgctcga cgccgcgcgc gagcgctacc cgatgctcgc cgagcgctgg cgaacgaagg cgggcgtgct gtcgggcggc gagcagcaga tgctcgcgct cgcgcgcgcg ttgatcggcg cgccgcgcgt gctgatcgcc gacgagccgg ccgaagggct cgcgccgctc gcggtcgagc aggttcgcgc gtgtctcgcc ggcgcgaang cgcaaggcgt cgcgctgctg ctgatcgagc agcggatgca gctcgcgccg gcgctcgcgt cgcgggtcgc ggtgatgggg cggggaagga tcgtctacga cggcccggcg nannngctcg atgcgggcat cgtcgagcgt tggctcagcg tcngntaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSALVELRG VRAGYGALPV LDGVDLRIAA GEAVALVGRN GSGRSTLAKA IMGMVRVAGT VRVDGIDVAG APTFAVARHR VGYVAESRDV FALLSVEDNL RLGLRGVSGA AARAALDAAR ERYPMLAERW RTKAGVLSGG EQQMLALARA LIGAPRVLIA DEPAEGLAPL AVEQVRACLA GAKAQGVALL LIEQRMQLAP ALASRVAVMG RGRIVYDGPA KALDAGIVER WLSVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgctc gtcgaattgc gcggcgtgcg cgccggatac ggcgcgctgc cggtgctcga cggcgtcgat ctgcggatcg cggcgggcga ggccgtcgcg ctcgtcgggc gcaacggctc gggccgctcg acgctcgcga aggcgatcat gggaatggtg cgggttgcgg ggacggtgcg cgtcgacggc atcgacgtcg cgggcgcgcc gacgttcgcg gtcgcgcggc atcgggtcgg ctacgtcgcc gagagccgcg acgtgttcgc gctcctgagc gtcgaggaca atctgcggct cggcctgcgc ggcgtgtcgg gcgccgccgc gcgcgcggcg ctcgacgccg cgcgcgagcg ctacccgatg ctcgccgagc gctggcgaac gaaggcgggc gtgctgtcgg gcggcgagca gcagatgctc gcgctcgcgc gcgcgttgat cggcgcgccg cgcgtgctga tcgccgacga gccggccgaa gggctcgcgc cgctcgcggt cgagcaggtt cgcgcgtgtc tcgccggcgc gaaggcgcaa ggcgtcgcgc tgctgctgat cgagcagcgg atgcagctcg cgccggcgct cgcgtcgcgg gtcgcggtga tggggcgggg aaggatcgtc tacgacggcc cggcgaaggc gctcgatgcg ggcatcgtcg agcgttggct cagcgtcggt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa