SSGCID: Target Page

ButhA.00057.d

sn-glycerol-3-phosphate import ATP-binding protein UgpC

CENTER ID:	ButhA.00057.d
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.d.A1.GE32700	Full length( ButhA.00057.d )		1	360

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5908
RefSeq:	YP_443522.1
UniProt:	Q2SU77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAALSLKGVR KSYDGAQYVL HGIDVDIADG EFVVLVGPSG CGKSTLLRMI AGLETVTEGE IAIGGRVVNT LEPKDRDIAM VFQNYALYPH MTVAQNMGYG LKIRGVERAL IDARVQAAAQ ILELGPLLAR RPRELSGGQR QRVAMGRAIV REPSVFLFDE PLSNLDAKLR VQMRLEIQRL HARLATTSVY VTHDQIEAMT LAQRVIVMNR GYAEQIGAPV DVYEKPATTF VASFIGSPAM NLLHGRLSED GAAFDIADGP RLPVAGAAGA GREIAPGREW ILGVRPEHMT PLPGAALATL AVDSCELLGA DNLAHGRWGV HDVAVRLPHA MRPARGETLP VALPAQHLHF FDPATGKRAG

NT Sequence

ATGGCCGCGC TGAGCTTGAA GGGCGTCAGG AAAAGTTACG ACGGCGCGCA GTACGTGCTG CACGGGATCG ACGTCGACAT CGCCGACGGC GAGTTCGTCG TGCTCGTCGG GCCGTCGGGC TGCGGGAAAT CGACGCTATT GCGGATGATC GCCGGCCTCG AGACGGTGAC GGAAGGCGAG ATCGCGATCG GCGGGCGCGT CGTCAATACG CTCGAGCCGA AGGATCGCGA CATCGCGATG GTGTTCCAGA ACTACGCGCT GTATCCGCAC ATGACGGTCG CGCAGAACAT GGGCTACGGG CTGAAGATCC GCGGCGTCGA GCGCGCGCTG ATCGACGCGC GCGTGCAGGC GGCCGCGCAG ATTCTCGAGC TCGGGCCGCT GCTCGCGCGG CGCCCGCGCG AGCTTTCGGG CGGCCAGCGG CAGCGCGTCG CGATGGGGCG CGCGATCGTG CGCGAGCCGT CGGTGTTCCT GTTCGACGAG CCGCTGTCGA ATCTCGATGC GAAGCTGCGC GTGCAGATGC GCCTCGAAAT CCAGCGGCTG CACGCGCGCC TCGCGACGAC GAGCGTCTAC GTGACGCACG ACCAGATCGA GGCGATGACG CTCGCGCAGC GCGTGATCGT GATGAATCGC GGCTATGCGG AGCAGATCGG CGCGCCCGTC GACGTGTACG AGAAGCCGGC GACGACGTTC GTCGCGAGCT TCATCGGCTC GCCGGCGATG AACCTGCTGC ACGGGCGGCT GTCGGAAGAC GGCGCGGCGT TCGATATCGC CGACGGTCCA CGGCTGCCGG TCGCGGGCGC GGCGGGCGCC GGCCGCGAGA TCGCGCCGGG GCGCGAATGG ATTCTCGGCG TGCGGCCCGA GCACATGACG CCGCTGCCAG GCGCGGCGCT CGCGACGCTC GCCGTCGATT CGTGCGAGCT GCTCGGCGCG GACAATCTCG CGCACGGCCG CTGGGGCGTG CACGACGTCG CGGTGCGGCT GCCGCACGCG ATGCGCCCGG CGCGCGGCGA GACGCTGCCC GTCGCGCTGC CCGCGCAACA TCTGCATTTC TTCGATCCGG CGACGGGCAA GCGCGCCGGT

Details for ButhA.00057.d.A1.GE32700

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	76

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAALSLKGV RKSYDGAQYV LHGIDVDIAD GEFVVLVGPS GCGKSTLLRM IAGLETVTEG EIAIGGRVVN TLEPKDRDIA MVFQNYALYP HMTVAQNMGY GLKIRGVERA LIDARVQAAA QILELGPLLA RRPRELSGGQ RQRVAMGRAI VREPSVFLFD EPLSNLDAKL RVQMRLEIQR LHARLATTSV YVTHDQIEAM TLAQRVIVMN RGYAEQIGAP VDVYEKPATT FVASFIGSXA MNLLHGRLXE DGAAFDIAXX XRLPVAGAAG

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg cgctgagctt gaagggcgtc aggaaaagtt acgacggcgc gcagtacgtg ctgcacggga tcgacgtcga catcgccgac ggcgagttcg tcgtgctcgt cgggccgtcg ggctgcggga aatcgacgct attgcggatg atcgccggcc tcgagacggt gacggaaggc gagatcgcga tcggcgggcg cgtcgtcaat acgctcgagc cgaaggatcg cgacatcgcg atggtgttcc agaactacgc gctgtatccg cacatgacgg tcgcgcagaa catgggctac gggctgaaga tccgcggcgt cgagcgcgcg ctgatcgacg cgcgcgtgca ggcggccgcg cagattctcg agctcgggcc gctgctcgcg cggcgcccgc gcgagctttc gggcggccag cggcagcgcg tcgcgatggg gcgcgcgatc gtgcgcgagc cgtcggtgtt cctgttcgac gagccgctgt cgaatctcga tgcgaagctg cgcgtgcaga tgcgcctcga aatccagcgg ctgcacgcgc gcctcgcgac gacgagcgtc tacgtgacgc acgaccagat cgaggcgatg acgctcgcgc agcgcgtgat cgtgatgaat cgcggctatg cggagcagat cggcgcgccc gtcgacgtgt acgagaagcc ggcgacgacg ttcgtcgcga gcttcatcgg ctcgnnngcg atgaacctgc tgcacgggcg gctgtnngaa gacggcgcgg cgttcgatat cgccnanngt nnncggctgc cggtcgcggg cgcggcgggc nc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcgctg agcttgaagg gcgtcaggaa aagttacgac ggcgcgcagt acgtgctgca cgggatcgac gtcgacatcg ccgacggcga gttcgtcgtg ctcgtcgggc cgtcgggctg cgggaaatcg acgctattgc ggatgatcgc cggcctcgag acggtgacgg aaggcgagat cgcgatcggc gggcgcgtcg tcaatacgct cgagccgaag gatcgcgaca tcgcgatggt gttccagaac tacgcgctgt atccgcacat gacggtcgcg cagaacatgg gctacgggct gaagatccgc ggcgtcgagc gcgcgctgat cgacgcgcgc gtgcaggcgg ccgcgcagat tctcgagctc gggccgctgc tcgcgcggcg cccgcgcgag ctttcgggcg gccagcggca gcgcgtcgcg atggggcgcg cgatcgtgcg cgagccgtcg gtgttcctgt tcgacgagcc gctgtcgaat ctcgatgcga agctgcgcgt gcagatgcgc ctcgaaatcc agcggctgca cgcgcgcctc gcgacgacga gcgtctacgt gacgcacgac cagatcgagg cgatgacgct cgcgcagcgc gtgatcgtga tgaatcgcgg ctatgcggag cagatcggcg cgcccgtcga cgtgtacgag aagccggcga cgacgttcgt cgcgagcttc atcggctcgc cggcgatgaa cctgctgcac gggcggctgt cggaagacgg cgcggcgttc gatatcgccg acggtccacg gctgccggtc gcgggcgcgg cgggcgccgg ccgcgagatc gcgccggggc gcgaatggat tctcggcgtg cggcccgagc acatgacgcc gctgccaggc gcggcgctcg cgacgctcgc cgtcgattcg tgcgagctgc tcggcgcgga caatctcgcg cacggccgct ggggcgtgca cgacgtcgcg gtgcggctgc cgcacgcgat gcgcccggcg cgcggcgaga cgctgcccgt cgcgctgccc gcgcaacatc tgcatttctt cgatccggcg acgggcaagc gcgccggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a