SSGCID: Target Page

ButhA.00057.b

Branched-chain amino acid ABC transporter, ATP-binding protein

CENTER ID:	ButhA.00057.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00057.b.A1.GE32698	Full length( ButhA.00057.b )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.6218
RefSeq:	YP_443822.1
UniProt:	Q2STC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNHSEREERE LNSVESGTPA LAIAGLEAWY GESHILHGVD LTVHRGEVVT LLGRNGAGRT TTLRAIMGLT GRRQGSIRIA GHETIDLPTH RIAHYGVGYC PEERGIFSSL SCEENLLLPP VLGDRKSAMS LEDIYEMFPN LASRRSSQGT RLSGGEQQML AVARILRTGA NLLLLDEISE GLAPVIVQAL ARMIVMLKAR GYTIVMVEQN FRFAAPLADR FYVMEHGRIV EHFGAAELEG KMPVLHDLLG V

NT Sequence

ATGAACCACA GCGAACGGGA AGAACGCGAG TTGAACAGCG TCGAAAGCGG CACGCCCGCG CTCGCGATCG CGGGGCTCGA AGCGTGGTAC GGCGAATCGC ACATCCTGCA CGGCGTCGAT CTGACCGTGC ATCGCGGCGA GGTCGTCACG CTGCTCGGCC GCAACGGCGC GGGCCGCACG ACGACGCTGC GCGCGATCAT GGGGCTCACG GGCCGCCGGC AGGGCTCGAT CAGGATCGCG GGCCACGAGA CGATCGATCT GCCGACGCAC AGGATCGCGC ATTACGGCGT GGGCTATTGC CCGGAGGAGC GCGGGATCTT CTCGAGCCTG TCGTGCGAGG AGAACCTGTT GCTGCCGCCC GTGCTCGGCG ATCGCAAGAG CGCGATGTCG CTCGAGGACA TCTACGAGAT GTTCCCGAAT CTCGCGTCCC GGCGCAGCAG CCAGGGCACG CGGCTGTCGG GCGGCGAGCA GCAGATGCTC GCGGTGGCGC GGATCCTGCG CACGGGCGCG AACCTGCTGT TGCTCGACGA GATCTCCGAG GGCCTCGCGC CCGTGATCGT GCAGGCGCTC GCGCGGATGA TCGTCATGCT GAAGGCGCGC GGCTACACGA TCGTGATGGT CGAGCAGAAC TTCCGTTTCG CGGCGCCGCT CGCGGATCGC TTCTACGTGA TGGAACACGG CCGCATCGTC GAGCATTTCG GCGCGGCCGA GCTCGAGGGG AAGATGCCCG TGCTGCACGA TCTGCTCGGC GTA

Details for ButhA.00057.b.A1.GE32698

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMNHSERE ERELNSVESG TPALAIAGLE AWYGESHILH GVDLTVHRGE VVTLLGRNGA GRTTTLRAIM GLTGRRQGSI RIAGHETIDL PTHRIAHYGV GYCPEERGIF SSLSCEENLL LPPVLGDRKS AMSLEDIYEM FPNLASRRSS QGTRLSGGEQ QMLAVARILR TGANLLLLDE ISEGLAPVIV QALARMIVML KARGYTIVMV EQNFRFAAPL ADRFYVMEHG RIVEHFGAAE LEGKMPVLHD LLGV

Validated NT Sequence

ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga accacagcga acgggaagaa cgcgagttga acagcgtcga aagcggcacg cccgcgctcg cgatcgcggg gctcgaagcg tggtacggcg aatcgcacat cctgcacggc gtcgatctga ccgtgcatcg cggcgaggtc gtcacgctgc tcggccgcaa cggcgcgggc cgcacgacga cgctgcgcgc gatcatgggg ctcacgggcc gccggcaggg ctcgatcagg atcgcgggcc acgagacgat cgatctgccg acgcacagga tcgcgcatta cggcgtgggc tattgcccgg aggagcgcgg gatcttctcg agcctgtcgt gcgaggagaa cctgttgctg ccgcccgtgc tcggcgatcg caagagcgcg atgtcgctcg aggacatcta cgagatgttc ccgaatctcg cgtcccggcg cagcagccag ggcacgcggc tgtcgggcgg cgagcagcag atgctcgcgg tggcgcggat cctgcgcacg ggcgcgaacc tgctgttgct cgacgagatc tccgagggcc tcgcgcccgt gatcgtgcag gcgctcgcgc ggatgatcgt catgctgaag gcgcgcggct acacgatcgt gatggtcgag cagaacttcc gtttcgcggc gccgctcgcg gatcgcttct acgtgatgga acacggccgc atcgtcgagc atttcggcgc ggccgagctc gaggggaaga tgcccgtgct gcacgatctg ctcggcgtat aataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNHSEREER ELNSVESGTP ALAIAGLEAW YGESHILHGV DLTVHRGEVV TLLGRNGAGR TTTLRAIMGL TGRRQGSIRI AGHETIDLPT HRIAHYGVGY CPEERGIFSS LSCEENLLLP PVLGDRKSAM SLEDIYEMFP NLASRRSSQG TRLSGGEQQM LAVARILRTG ANLLLLDEIS EGLAPVIVQA LARMIVMLKA RGYTIVMVEQ NFRFAAPLAD RFYVMEHGRI VEHFGAAELE GKMPVLHDLL GV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccacagc gaacgggaag aacgcgagtt gaacagcgtc gaaagcggca cgcccgcgct cgcgatcgcg gggctcgaag cgtggtacgg cgaatcgcac atcctgcacg gcgtcgatct gaccgtgcat cgcggcgagg tcgtcacgct gctcggccgc aacggcgcgg gccgcacgac gacgctgcgc gcgatcatgg ggctcacggg ccgccggcag ggctcgatca ggatcgcggg ccacgagacg atcgatctgc cgacgcacag gatcgcgcat tacggcgtgg gctattgccc ggaggagcgc gggatcttct cgagcctgtc gtgcgaggag aacctgttgc tgccgcccgt gctcggcgat cgcaagagcg cgatgtcgct cgaggacatc tacgagatgt tcccgaatct cgcgtcccgg cgcagcagcc agggcacgcg gctgtcgggc ggcgagcagc agatgctcgc ggtggcgcgg atcctgcgca cgggcgcgaa cctgctgttg ctcgacgaga tctccgaggg cctcgcgccc gtgatcgtgc aggcgctcgc gcggatgatc gtcatgctga aggcgcgcgg ctacacgatc gtgatggtcg agcagaactt ccgtttcgcg gcgccgctcg cggatcgctt ctacgtgatg gaacacggcc gcatcgtcga gcatttcggc gcggccgagc tcgaggggaa gatgcccgtg ctgcacgatc tgctcggcgt ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa