SSGCID: Target Page

ButhA.00050.b

GDP-6-deoxy-D-lyxo-4-hexulose reductase, putative

CENTER ID:	ButhA.00050.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00050.b.B1.GE36047	Full length( ButhA.00050.b )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00050.b.B1.PS01730	Full length( ButhA.00050.b )		1	322

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.777
RefSeq:	YP_438888.1
UniProt:	Q2T7G1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPSEPGARR ALITGIAGFT GRHLAARLEA DGYEVWGTVA PDADLLGGPP LRRWRCVKAD LLDVDSLHAA VADARPHAVV HLAARAHVAH GNPRDTYLVN VVGTRNLLAS LAGLDARPHA VLLASSANVY GNAPIEVLDE SAPPLPANDY AVSKLAMEYM AKLWLDRLPI TIARPFNYTG VGQSDAYLLP KLVAHYARGE PRISLGNLDV SRDFSDVRDV VDAYARLVDA APAGETFNVC AERGYALKEV LAMLARIAGY VIDVSVDPRF VRANEVKKLV GSCRKLRGAI GETRRTPLDE TLRWMHDDMR AALAARAGQP AS

NT Sequence

ATGAAACCTT CTGAGCCCGG CGCGCGGCGC GCGCTCATCA CCGGCATCGC GGGCTTCACC GGCCGCCACC TCGCCGCGCG CCTCGAGGCG GACGGCTACG AAGTGTGGGG CACGGTCGCG CCCGACGCCG ACCTGCTCGG CGGCCCGCCG CTGCGGCGCT GGCGCTGCGT GAAGGCGGAC CTGCTCGACG TCGACTCGCT GCACGCGGCG GTCGCCGACG CGCGGCCGCA CGCGGTCGTG CATCTGGCCG CGCGCGCGCA CGTCGCGCAC GGCAATCCGC GGGACACGTA CCTCGTGAAC GTGGTCGGCA CGCGCAACCT GCTCGCGTCG CTCGCGGGCC TCGACGCGCG TCCGCACGCG GTGCTGCTCG CGAGCAGCGC GAACGTGTAC GGCAACGCGC CGATCGAAGT GCTCGACGAA TCCGCGCCGC CGCTGCCCGC GAACGACTAC GCGGTCAGCA AGCTCGCGAT GGAATACATG GCGAAGCTCT GGCTCGACCG CTTGCCGATC ACGATCGCGC GGCCGTTCAA CTACACGGGC GTCGGCCAGA GCGACGCGTA TTTGCTGCCG AAGCTCGTCG CGCATTACGC GCGCGGCGAG CCGCGGATCT CGCTCGGCAA TCTCGACGTG AGCCGCGATT TCTCCGACGT GCGCGACGTC GTCGACGCCT ACGCGCGGCT CGTCGACGCC GCGCCCGCGG GCGAGACGTT CAACGTGTGC GCGGAACGCG GCTATGCGTT GAAGGAAGTG CTGGCGATGC TCGCGCGGAT CGCGGGCTAC GTGATCGACG TCAGCGTCGA TCCGCGCTTC GTGCGTGCGA ACGAGGTGAA GAAGCTGGTC GGCTCGTGCC GGAAGCTGCG CGGCGCGATC GGCGAAACGC GCCGCACGCC GCTCGACGAG ACGCTGCGCT GGATGCACGA CGACATGCGC GCGGCGCTCG CCGCACGCGC GGGTCAGCCG GCGAGC

Details for ButhA.00050.b.B1.GE36047

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	103

Validated AA Sequence

MAHHHHHHMK PSEPGARRAL ITGIAGFTGR HLAARLEADG YEVWGTVAPD ADLLGGPPLR RWRCVKADLL DVDSLHAAVA DARPHAVVHL AARAHVAHGN PRDTYLVNVV GTRNLLASLA GLDARPHAVL LASSANVYGN APIEVLDESA PPLPANDYAV SKLAMEYMAK LWLDRLPITI ARPFNYTGVG QSDAYLLPKL VAHYARGEPR ISLGNLDVSR DFSDVRDVVD AYARLVDAAP AGETFNVCAE RGYALXEVLA MLARIAGYVI DVSVDPRFXR ANEVKKLVXS CXKLRGAIGX TRXTXLDETL RWMHDDMXXR XPHAXVXXXX XXVXXSGXXX XXX

Validated NT Sequence

ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgaaacct tctgagcccg gcgcgcggcg cgcgctcatc accggcatcg cgggcttcac cggccgccac ctcgccgcgc gcctcgaggc ggacggctac gaagtgtggg gcacggtcgc gcccgacgcc gacctgctcg gcggcccgcc gctgcggcgc tggcgctgcg tgaaggcgga cctgctcgac gtcgactcgc tgcacgcggc ggtcgccgac gcgcggccgc acgcggtcgt gcatctggcc gcgcgcgcgc acgtcgcgca cggcaatccg cgggacacgt acctcgtgaa cgtggtcggc acgcgcaacc tgctcgcgtc gctcgcgggc ctcgacgcgc gtccgcacgc ggtgctgctc gcgagcagcg cgaacgtgta cggcaacgcg ccgatcgaag tgctcgacga atccgcgccg ccgctgcccg cgaacgacta cgcggtcagc aagctcgcga tggaatacat ggcgaagctc tggctcgacc gcttgccgat cacgatcgcg cggccgttca actacacggg cgtcggccag agcgacgcgt atttgctgcc gaagctcgtc gcgcattacg cgcgcggcga gccgcggatc tcgctcggca atctcgacgt gagccgcgat ttctccgacg tgcgcgacgt cgtcgacgcc tacgcgcggc tcgtcgacgc cgcgcccgcg ggcgagacgt tcaacgtgtg cgcggaacgc ggctatgcgt tgaangaagt gctggcgatg ctcgcgcgga tcgcgggcta cgtgatcgac gtcagcgtcg atccgcgctt cntgcgtgcg aacgaggtga agaagctggt cnnctcgtgc nggaagctgc gcggcgcgat cggcnaaacg cgccncacgc ngctcgacga gacgctgcgc tggatgcacg acgacatgnn gcngcgctng ccgcacgcgn nggtcannnn nnnanctaan ngagtannnn natcnggcng cnnnnnnnan nccnnnaa

Expected Protein Sequence

MAHHHHHHMK PSEPGARRAL ITGIAGFTGR HLAARLEADG YEVWGTVAPD ADLLGGPPLR RWRCVKADLL DVDSLHAAVA DARPHAVVHL AARAHVAHGN PRDTYLVNVV GTRNLLASLA GLDARPHAVL LASSANVYGN APIEVLDESA PPLPANDYAV SKLAMEYMAK LWLDRLPITI ARPFNYTGVG QSDAYLLPKL VAHYARGEPR ISLGNLDVSR DFSDVRDVVD AYARLVDAAP AGETFNVCAE RGYALKEVLA MLARIAGYVI DVSVDPRFVR ANEVKKLVGS CRKLRGAIGE TRRTPLDETL RWMHDDMRAA LAARAGQPAS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cttctgagcc cggcgcgcgg cgcgcgctca tcaccggcat cgcgggcttc accggccgcc acctcgccgc gcgcctcgag gcggacggct acgaagtgtg gggcacggtc gcgcccgacg ccgacctgct cggcggcccg ccgctgcggc gctggcgctg cgtgaaggcg gacctgctcg acgtcgactc gctgcacgcg gcggtcgccg acgcgcggcc gcacgcggtc gtgcatctgg ccgcgcgcgc gcacgtcgcg cacggcaatc cgcgggacac gtacctcgtg aacgtggtcg gcacgcgcaa cctgctcgcg tcgctcgcgg gcctcgacgc gcgtccgcac gcggtgctgc tcgcgagcag cgcgaacgtg tacggcaacg cgccgatcga agtgctcgac gaatccgcgc cgccgctgcc cgcgaacgac tacgcggtca gcaagctcgc gatggaatac atggcgaagc tctggctcga ccgcttgccg atcacgatcg cgcggccgtt caactacacg ggcgtcggcc agagcgacgc gtatttgctg ccgaagctcg tcgcgcatta cgcgcgcggc gagccgcgga tctcgctcgg caatctcgac gtgagccgcg atttctccga cgtgcgcgac gtcgtcgacg cctacgcgcg gctcgtcgac gccgcgcccg cgggcgagac gttcaacgtg tgcgcggaac gcggctatgc gttgaaggaa gtgctggcga tgctcgcgcg gatcgcgggc tacgtgatcg acgtcagcgt cgatccgcgc ttcgtgcgtg cgaacgaggt gaagaagctg gtcggctcgt gccggaagct gcgcggcgcg atcggcgaaa cgcgccgcac gccgctcgac gagacgctgc gctggatgca cgacgacatg cgcgcggcgc tcgccgcacg cgcgggtcag ccggcgagct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for ButhA.00050.b.B1.PS01730

PURIFICATION DATe:	1/22/2013
CONCENTRATION:	51.7mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	94
PERCENT COVERAGE:	103

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMK PSEPGARRAL ITGIAGFTGR HLAARLEADG YEVWGTVAPD ADLLGGPPLR RWRCVKADLL DVDSLHAAVA DARPHAVVHL AARAHVAHGN PRDTYLVNVV GTRNLLASLA GLDARPHAVL LASSANVYGN APIEVLDESA PPLPANDYAV SKLAMEYMAK LWLDRLPITI ARPFNYTGVG QSDAYLLPKL VAHYARGEPR ISLGNLDVSR DFSDVRDVVD AYARLVDAAP AGETFNVCAE RGYALKEVLA MLARIAGYVI DVSVDPRFVR ANEVKKLVGS CRKLRGAIGE TRRTPLDETL RWMHDDMRAA LAARAGQPAS

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC CTTCTGAGCC CGGCGCGCGG CGCGCGCTCA TCACCGGCAT CGCGGGCTTC ACCGGCCGCC ACCTCGCCGC GCGCCTCGAG GCGGACGGCT ACGAAGTGTG GGGCACGGTC GCGCCCGACG CCGACCTGCT CGGCGGCCCG CCGCTGCGGC GCTGGCGCTG CGTGAAGGCG GACCTGCTCG ACGTCGACTC GCTGCACGCG GCGGTCGCCG ACGCGCGGCC GCACGCGGTC GTGCATCTGG CCGCGCGCGC GCACGTCGCG CACGGCAATC CGCGGGACAC GTACCTCGTG AACGTGGTCG GCACGCGCAA CCTGCTCGCG TCGCTCGCGG GCCTCGACGC GCGTCCGCAC GCGGTGCTGC TCGCGAGCAG CGCGAACGTG TACGGCAACG CGCCGATCGA AGTGCTCGAC GAATCCGCGC CGCCGCTGCC CGCGAACGAC TACGCGGTCA GCAAGCTCGC GATGGAATAC ATGGCGAAGC TCTGGCTCGA CCGCTTGCCG ATCACGATCG CGCGGCCGTT CAACTACACG GGCGTCGGCC AGAGCGACGC GTATTTGCTG CCGAAGCTCG TCGCGCATTA CGCGCGCGGC GAGCCGCGGA TCTCGCTCGG CAATCTCGAC GTGAGCCGCG ATTTCTCCGA CGTGCGCGAC GTCGTCGACG CCTACGCGCG GCTCGTCGAC GCCGCGCCCG CGGGCGAGAC GTTCAACGTG TGCGCGGAAC GCGGCTATGC GTTGAAGGAA GTGCTGGCGA TGCTCGCGCG GATCGCGGGC TACGTGATCG ACGTCAGCGT CGATCCGCGC TTCGTGCGTG CGAACGAGGT GAAGAAGCTG GTCGGCTCGT GCCGGAAGCT GCGCGGCGCG ATCGGCGAAA CGCGCCGCAC GCCGCTCGAC GAGACGCTGC GCTGGATGCA CGACGACATG CGCGCGGCGC TCGCCGCACG CGCGGGTCAG CCGGCGAGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT