SSGCID: Target Page

ButhA.00050.a

GDP-mannose 4,6-dehydratase

CENTER ID:	ButhA.00050.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00050.a.B1.GE36046	Full length( ButhA.00050.a )		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4100
RefSeq:	YP_441878.1
UniProt:	Q2SYX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVIAIITGI TGQDGAYLAQ LLLDKGYVVH GTYRRTSSVN FWRIEELGIG AHPNLHLVEY DLTDLSASIR LLRTTGATEV YNLAAQSFVG VSFDQPVTTA EITGIGPLNL LEAIRIVNPA IRFYQASTSE MFGKVQAIPQ TETTPFYPRS PYGVAKLYAH WITVNYRESY NIFGCSGILF NHESPLRGRE FVTRKITDSM AKIRLGKLDV LELGNLDAKR DWGFAKEYVE GMWRMLQADK PDTYVLATNR TEKVRDFVGM AARAAGFKLA WEGREENEVG IDLGSGKTIV RINPKFYRPA EVDLLIGDPK KARDELGWAP ATTLEQLCQM MVEADIRRNE TGFSF

NT Sequence

ATGAGCGTCA TTGCAATTAT CACCGGCATC ACGGGCCAAG ACGGGGCTTA TCTCGCTCAA CTCCTGCTCG ACAAGGGCTA TGTGGTTCAC GGCACCTATC GCCGCACCAG CTCGGTCAAC TTCTGGCGCA TCGAGGAGCT CGGCATCGGC GCTCATCCGA ATCTCCATCT CGTCGAATAC GATCTGACCG ACCTGAGCGC GAGCATCCGC CTGCTGCGGA CGACGGGCGC GACGGAAGTT TACAATCTTG CCGCGCAAAG CTTCGTCGGC GTATCGTTCG ACCAGCCCGT CACCACCGCG GAGATCACCG GCATCGGCCC GCTGAACCTG CTCGAGGCGA TCCGGATCGT GAATCCGGCG ATCCGCTTCT ATCAGGCGAG CACGTCGGAG ATGTTCGGCA AGGTGCAGGC GATTCCGCAG ACGGAGACGA CGCCGTTCTA TCCGCGCAGC CCATACGGCG TCGCGAAGCT CTATGCGCAC TGGATCACGG TCAACTATCG CGAAAGCTAC AACATCTTCG GCTGCAGCGG GATTCTGTTC AATCACGAAT CGCCGCTGCG CGGCCGCGAA TTCGTCACGC GCAAGATCAC CGACAGCATG GCGAAGATCC GCCTCGGCAA GCTCGACGTG CTCGAGCTAG GCAATCTCGA CGCGAAGCGC GACTGGGGCT TCGCGAAGGA ATACGTGGAA GGGATGTGGC GGATGCTGCA GGCCGACAAG CCCGATACCT ACGTGCTCGC GACGAACCGG ACCGAGAAGG TGCGGGACTT CGTCGGCATG GCGGCGCGCG CGGCGGGCTT CAAGCTTGCA TGGGAAGGCC GCGAAGAAAA CGAAGTCGGG ATCGACCTGG GCTCGGGCAA GACGATCGTG AGGATCAATC CGAAGTTCTA TCGGCCGGCC GAAGTGGATC TGCTGATCGG CGATCCGAAG AAGGCGCGCG ACGAGCTCGG GTGGGCGCCG GCCACGACAC TGGAGCAGTT GTGCCAGATG ATGGTCGAGG CCGACATCCG GCGCAATGAA ACAGGGTTTT CCTTC

Details for ButhA.00050.a.B1.GE36046

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttagaagga aaaccctgtt tcattgcgcc ggatgtcggc ctcgaccatc atctggcaca actgctccag tgtcgtggcc ggcgcccacc cgagctcgtc gcgcgccttc ttcggatcgc cgatcagcag atccacttcg gccggccgat agaacttcgg attgatcctc acgatcgtct tgcccgagcc caggtcgatc ccgacttcgt tttcttcgcg gccttcccat gcaagcttga agcccgccgc gcgcgccgcc atgccgacga agtcccgcac cttctcggtc cggttcgtcg cgagcacgta ggtatcgggc ttgtcggcct gcagcatccg ccacatccct tccacgtatt ccttcgcgaa gccccagtcg cgcttcgcgt cgagattgcc tagctcgagc acgtcgagct tgccgaggcg gatcttcgcc atgctgtcgg tgatcttgcg cgtgacgaat tcgcggccgc gcagcggcga ttcgtgattg aacagaatcc cgctgcagcc gaagatgttg tagctttcgc gatagttgac cgtgatccag tgcgcataga gcttcgcgac gccgtatggg ctgcgcggat agaacggcgt cgtctccgtc tgcggaatcg cctgcacctt gccgaacatc tccgacgtgc tcgcctgata gaagcggatc gccggattca cgatccggat cgcctcgagc aggttcagcg ggccgatgcc ggtgatctcc gcggtggtga cgggctggtc gaacgatacg ccgacgaagc tttgcgcggc aagattgtaa acttccgtcg cgcccgtcgt ccgcagcagg cggatgctcg cgctcaggtc ggtcagatcg tattcgacga gatggagatt cggatgagcg ccgatgccga gctcctcgat gcgccagaag ttgaccgagc tggtgcggcg ataggtgccg tgaaccacat agcccttgtc gagcaggagt tgagcgagat aagccccgtc ttggcccgtg atgccggtga taattgcaat gacgctcata tggtggtggt gg

Expected Protein Sequence

MAHHHHHHMS VIAIITGITG QDGAYLAQLL LDKGYVVHGT YRRTSSVNFW RIEELGIGAH PNLHLVEYDL TDLSASIRLL RTTGATEVYN LAAQSFVGVS FDQPVTTAEI TGIGPLNLLE AIRIVNPAIR FYQASTSEMF GKVQAIPQTE TTPFYPRSPY GVAKLYAHWI TVNYRESYNI FGCSGILFNH ESPLRGREFV TRKITDSMAK IRLGKLDVLE LGNLDAKRDW GFAKEYVEGM WRMLQADKPD TYVLATNRTE KVRDFVGMAA RAAGFKLAWE GREENEVGID LGSGKTIVRI NPKFYRPAEV DLLIGDPKKA RDELGWAPAT TLEQLCQMMV EADIRRNETG FSF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcattgcaat tatcaccggc atcacgggcc aagacggggc ttatctcgct caactcctgc tcgacaaggg ctatgtggtt cacggcacct atcgccgcac cagctcggtc aacttctggc gcatcgagga gctcggcatc ggcgctcatc cgaatctcca tctcgtcgaa tacgatctga ccgacctgag cgcgagcatc cgcctgctgc ggacgacggg cgcgacggaa gtttacaatc ttgccgcgca aagcttcgtc ggcgtatcgt tcgaccagcc cgtcaccacc gcggagatca ccggcatcgg cccgctgaac ctgctcgagg cgatccggat cgtgaatccg gcgatccgct tctatcaggc gagcacgtcg gagatgttcg gcaaggtgca ggcgattccg cagacggaga cgacgccgtt ctatccgcgc agcccatacg gcgtcgcgaa gctctatgcg cactggatca cggtcaacta tcgcgaaagc tacaacatct tcggctgcag cgggattctg ttcaatcacg aatcgccgct gcgcggccgc gaattcgtca cgcgcaagat caccgacagc atggcgaaga tccgcctcgg caagctcgac gtgctcgagc taggcaatct cgacgcgaag cgcgactggg gcttcgcgaa ggaatacgtg gaagggatgt ggcggatgct gcaggccgac aagcccgata cctacgtgct cgcgacgaac cggaccgaga aggtgcggga cttcgtcggc atggcggcgc gcgcggcggg cttcaagctt gcatgggaag gccgcgaaga aaacgaagtc gggatcgacc tgggctcggg caagacgatc gtgaggatca atccgaagtt ctatcggccg gccgaagtgg atctgctgat cggcgatccg aagaaggcgc gcgacgagct cgggtgggcg ccggccacga cactggagca gttgtgccag atgatggtcg aggccgacat ccggcgcaat gaaacagggt tttccttctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat