ButhA.00047.a

Fumarate hydratase, class II

CENTER ID: ButhA.00047.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00047.a.B1.GE36045 Full length( ButhA.00047.a ) 1 620
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4474
RefSeq: YP_442222.2
UniProt: Q2SXX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHRSSFTRPF RRKMPDASRA RLIRGRDARD SGDLTSANAV RRDSPHMAEP AKRPRRPHRA RTLAAQRLHA ERSHPSAAAR AMLACIAPPR SSRCACALFA HPSAPTPVSP IARDAARNRT RRYACPSVDA VARTAPSPHA HCAAPARRPP SSEGAIMSEA VRMERDTFGE IAVPAARLWG AQTQRSLQNF KISTEKQSPE LIHALALIKR AAAAVNLELG VLAQDKANAI IATADEIIAG RHPDEFPLAV WQTGSGTQTN MNLNEVIANR ASELLGGERG ESRNVHPNDD VNRGQSSNDV FPTAMHVAAA RAIVDHLLPA LRTLRATLDA KAAAFADIVK IGRTHLQDAT PLTLGQEFSG YVAQLDQGIR HVETALPHLY ELAQGGTAVG TGLNAHPKFA AGVAAEIGRL TGLPFVSAPN KFEVMAAADA LVFAHGALKT VAASLMKIAN DIRWLASGPR CGLGELSIPE NEPGSSIMPG KVNPTQSEAV TMLCCQVFGN DVAVNFGGAS GNFELNVFRP MIAHNVLQSV RLLADGAQSF NDHCAVGIEP NRTRIDALLN ESLMLVTALN PHIGYDKAAQ IAKKAHKEGT TLRAAALALG YVTDAQFDEW VRPAKMVGNS
NT Sequence
ATGCACCGGT CCTCTTTTAC GCGCCCTTTT CGTCGGAAGA TGCCGGATGC CTCGCGAGCG CGGCTGATTC GCGGGCGCGA CGCCCGCGAC AGCGGCGACC TCACCTCCGC GAACGCGGTT CGCCGCGATT CGCCGCACAT GGCGGAGCCG GCAAAGCGGC CGCGTCGCCC ACATCGCGCA CGCACGCTCG CGGCGCAGCG TCTCCACGCG GAACGATCGC ATCCGTCTGC CGCCGCGCGA GCGATGCTCG CGTGCATCGC GCCGCCCCGA TCGTCGCGAT GCGCATGCGC GCTGTTCGCT CATCCGAGTG CCCCCACGCC CGTCTCGCCG ATCGCCCGCG ACGCGGCACG AAATCGCACA CGGCGCTATG CTTGCCCATC AGTGGACGCC GTCGCGAGAA CGGCTCCATC CCCGCATGCG CATTGCGCCG CGCCCGCGCG GCGTCCCCCG TCATCTGAAG GAGCAATCAT GAGCGAAGCA GTACGGATGG AACGCGACAC GTTCGGCGAA ATCGCAGTGC CGGCCGCCCG GCTATGGGGC GCGCAGACGC AGCGCTCGCT GCAGAACTTC AAGATCTCGA CCGAAAAGCA GTCGCCCGAA CTGATTCACG CGCTCGCGCT GATCAAGCGC GCGGCGGCCG CCGTCAATCT CGAACTCGGC GTGCTCGCGC AGGACAAGGC GAACGCGATC ATCGCCACCG CCGACGAGAT CATCGCCGGC CGTCATCCCG ATGAATTCCC GCTCGCCGTC TGGCAAACGG GTTCGGGCAC GCAGACCAAC ATGAACCTCA ACGAGGTGAT CGCGAACCGC GCGAGCGAGT TGCTCGGCGG CGAGCGAGGC GAAAGCCGCA ACGTGCATCC GAACGACGAC GTCAATCGCG GCCAGTCGTC GAACGACGTG TTCCCGACCG CGATGCACGT CGCCGCCGCG CGCGCGATCG TCGACCATCT GCTGCCCGCG CTGCGCACGC TGCGCGCGAC GCTCGACGCG AAGGCCGCCG CGTTTGCCGA CATCGTGAAG ATCGGCCGCA CGCACCTGCA GGACGCGACG CCGCTCACGC TCGGCCAGGA ATTCTCCGGC TACGTCGCTC AGCTCGATCA GGGCATTCGC CACGTCGAGA CGGCGCTGCC CCATCTGTAC GAGCTCGCGC AAGGCGGCAC GGCGGTCGGC ACGGGCCTGA ACGCGCATCC GAAGTTCGCG GCGGGCGTCG CGGCCGAGAT CGGCCGGCTC ACCGGCTTGC CGTTCGTCAG TGCGCCGAAC AAATTCGAAG TGATGGCCGC GGCCGACGCG CTCGTGTTCG CGCACGGCGC GCTTAAGACG GTCGCCGCGA GCCTCATGAA GATCGCGAAC GACATCCGCT GGCTCGCGAG CGGGCCGCGT TGCGGGCTCG GCGAGTTGTC GATTCCGGAG AACGAGCCGG GCAGCTCGAT CATGCCGGGC AAGGTCAATC CGACGCAATC GGAAGCGGTG ACGATGCTCT GCTGTCAAGT GTTCGGCAAC GACGTCGCGG TGAACTTCGG CGGCGCGAGC GGCAATTTCG AACTGAACGT GTTCCGGCCG ATGATTGCGC ACAACGTACT GCAATCGGTG CGGCTGCTCG CGGACGGCGC GCAGAGCTTC AACGATCACT GCGCGGTCGG CATCGAGCCG AATCGCACGC GCATCGACGC ACTCCTGAAC GAATCGCTGA TGCTCGTGAC GGCGCTCAAT CCGCACATCG GCTACGACAA GGCGGCGCAG ATCGCGAAGA AGGCGCACAA GGAAGGCACG ACGCTCAGAG CCGCCGCGCT CGCGCTCGGT TACGTGACCG ATGCGCAATT CGACGAATGG GTGCGGCCGG CGAAGATGGT CGGCAATAGC
Details for ButhA.00047.a.B1.GE36045
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMH RSSFTRPFRR KMPDASRARL IRGRDARDSG DLTSANAVRR DSPHMAEPAK RPRRPHRART LAAQRLHAER SHPSAAARAM LACIAPPRSS RCACALFAHP SAPTPVSPIA RDAARNRTRR YACPSVDAVA RTAPSPHAHC AAPARRPPSS EGAIMSEAVR MERDTFGEIA VPAARLWGAQ TQRSLQNFKI STEKQSPELI HALALIKRAA AAVNLELGVL AQDKANAIIA TADEIIAGRH PDEFPLAVWQ TGSGTQTNMN LNEVIANRAS ELLGGERGES RNVHPNDDVN RGQSSNDVFP XAMHVAXXAR SSTSAXRXXX AXXXARXXXX XXXSXXXXXX XXXXXXXXXX TXXDXXXXXX XXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgcac cggtcctctt ttacgcgccc ttttcgtcgg aagatgccgg atgcctcgcg agcgcggctg attcgcgggc gcgacgcccg cgacagcggc gacctcacct ccgcgaacgc ggttcgccgc gattcgccgc acatggcgga gccggcaaag cggccgcgtc gcccacatcg cgcacgcacg ctcgcggcgc agcgtctcca cgcggaacga tcgcatccgt ctgccgccgc gcgagcgatg ctcgcgtgca tcgcgccgcc ccgatcgtcg cgatgcgcat gcgcgctgtt cgctcatccg agtgccccca cgcccgtctc gccgatcgcc cgcgacgcgg cacgaaatcg cacacggcgc tatgcttgcc catcagtgga cgccgtcgcg agaacggctc catccccgca tgcgcattgc gccgcgcccg cgcggcgtcc cccgtcatct gaaggagcaa tcatgagcga agcagtacgg atggaacgcg acacgttcgg cgaaatcgca gtgccggccg cccggctatg gggcgcgcag acgcagcgct cgctgcagaa cttcaagatc tcgaccgaaa agcagtcgcc cgaactgatt cacgcgctcg cgctgatcaa gcgcgcggcg gccgccgtca atctcgaact cggcgtgctc gcgcaggaca aggcgaacgc gatcatcgcc accgccgacg agatcatcgc cggccgtcat cccgatgaat tcccgctcgc cgtctggcaa acgggttcgg gcacgcagac caacatgaac ctcaacgagg tgatcgcgaa ccgcgcgagc gagttgctcg gcggcgagcg aggcgaaagc cgcaacgtgc atccgaacga cgacgtcaat cgcggncagt cgtcgaacga cgtgttcccg ancgcgatgc acgtcgccnc nngcgcgcga tcgtcgacnt ctgctgnccg nncnncgnnn gctncnnnng angctcgacn nnnnnnnnnn nnnnnnnnnt cnnnnnnann cnncngnnnc nnnnnnnntn nncnnnnnnn nnnnnnnnnt acnnnnnnng atnnngnnnn nnnnnnnnnn nnnnnnnnnc ntnnnnnnnn nnnngnng
Expected Protein Sequence
MAHHHHHHMH RSSFTRPFRR KMPDASRARL IRGRDARDSG DLTSANAVRR DSPHMAEPAK RPRRPHRART LAAQRLHAER SHPSAAARAM LACIAPPRSS RCACALFAHP SAPTPVSPIA RDAARNRTRR YACPSVDAVA RTAPSPHAHC AAPARRPPSS EGAIMSEAVR MERDTFGEIA VPAARLWGAQ TQRSLQNFKI STEKQSPELI HALALIKRAA AAVNLELGVL AQDKANAIIA TADEIIAGRH PDEFPLAVWQ TGSGTQTNMN LNEVIANRAS ELLGGERGES RNVHPNDDVN RGQSSNDVFP TAMHVAAARA IVDHLLPALR TLRATLDAKA AAFADIVKIG RTHLQDATPL TLGQEFSGYV AQLDQGIRHV ETALPHLYEL AQGGTAVGTG LNAHPKFAAG VAAEIGRLTG LPFVSAPNKF EVMAAADALV FAHGALKTVA ASLMKIANDI RWLASGPRCG LGELSIPENE PGSSIMPGKV NPTQSEAVTM LCCQVFGNDV AVNFGGASGN FELNVFRPMI AHNVLQSVRL LADGAQSFND HCAVGIEPNR TRIDALLNES LMLVTALNPH IGYDKAAQIA KKAHKEGTTL RAAALALGYV TDAQFDEWVR PAKMVGNS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcacc ggtcctcttt tacgcgccct tttcgtcgga agatgccgga tgcctcgcga gcgcggctga ttcgcgggcg cgacgcccgc gacagcggcg acctcacctc cgcgaacgcg gttcgccgcg attcgccgca catggcggag ccggcaaagc ggccgcgtcg cccacatcgc gcacgcacgc tcgcggcgca gcgtctccac gcggaacgat cgcatccgtc tgccgccgcg cgagcgatgc tcgcgtgcat cgcgccgccc cgatcgtcgc gatgcgcatg cgcgctgttc gctcatccga gtgcccccac gcccgtctcg ccgatcgccc gcgacgcggc acgaaatcgc acacggcgct atgcttgccc atcagtggac gccgtcgcga gaacggctcc atccccgcat gcgcattgcg ccgcgcccgc gcggcgtccc ccgtcatctg aaggagcaat catgagcgaa gcagtacgga tggaacgcga cacgttcggc gaaatcgcag tgccggccgc ccggctatgg ggcgcgcaga cgcagcgctc gctgcagaac ttcaagatct cgaccgaaaa gcagtcgccc gaactgattc acgcgctcgc gctgatcaag cgcgcggcgg ccgccgtcaa tctcgaactc ggcgtgctcg cgcaggacaa ggcgaacgcg atcatcgcca ccgccgacga gatcatcgcc ggccgtcatc ccgatgaatt cccgctcgcc gtctggcaaa cgggttcggg cacgcagacc aacatgaacc tcaacgaggt gatcgcgaac cgcgcgagcg agttgctcgg cggcgagcga ggcgaaagcc gcaacgtgca tccgaacgac gacgtcaatc gcggccagtc gtcgaacgac gtgttcccga ccgcgatgca cgtcgccgcc gcgcgcgcga tcgtcgacca tctgctgccc gcgctgcgca cgctgcgcgc gacgctcgac gcgaaggccg ccgcgtttgc cgacatcgtg aagatcggcc gcacgcacct gcaggacgcg acgccgctca cgctcggcca ggaattctcc ggctacgtcg ctcagctcga tcagggcatt cgccacgtcg agacggcgct gccccatctg tacgagctcg cgcaaggcgg cacggcggtc ggcacgggcc tgaacgcgca tccgaagttc gcggcgggcg tcgcggccga gatcggccgg ctcaccggct tgccgttcgt cagtgcgccg aacaaattcg aagtgatggc cgcggccgac gcgctcgtgt tcgcgcacgg cgcgcttaag acggtcgccg cgagcctcat gaagatcgcg aacgacatcc gctggctcgc gagcgggccg cgttgcgggc tcggcgagtt gtcgattccg gagaacgagc cgggcagctc gatcatgccg ggcaaggtca atccgacgca atcggaagcg gtgacgatgc tctgctgtca agtgttcggc aacgacgtcg cggtgaactt cggcggcgcg agcggcaatt tcgaactgaa cgtgttccgg ccgatgattg cgcacaacgt actgcaatcg gtgcggctgc tcgcggacgg cgcgcagagc ttcaacgatc actgcgcggt cggcatcgag ccgaatcgca cgcgcatcga cgcactcctg aacgaatcgc tgatgctcgt gacggcgctc aatccgcaca tcggctacga caaggcggcg cagatcgcga agaaggcgca caaggaaggc acgacgctca gagccgccgc gctcgcgctc ggttacgtga ccgatgcgca attcgacgaa tgggtgcggc cggcgaagat ggtcggcaat agctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat