ButhA.00046.a

Alpha,alpha-trehalose-phosphate synthase

CENTER ID: ButhA.00046.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00046.a.B1.GE36044 Full length( ButhA.00046.a ) 1 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4539
RefSeq: YP_442285.1
UniProt: Q2SXR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLIIVSNR VAPISEGEPA AGGLAIGVYD ALKETGGTWF GWSGEVVASG APQIRVEEHG PVTFATIGLS RRDYDQYYRG FSNATLWPAF HYRADLIQFD RHEFEGYSRV NVWLARQLVP LLRDDDVIWV HDYHLIPFAQ ALRAAGVKNR IGFFLHIPFP AAQVLVNVPP HRELVEALCS FDLLGFQTDT DLRAFFEYVE CEAGGAVDRA AHPARVEAFG RTLRAAAYPI GVYPDEIAAL AKAGENGRAV KALAASLRGR QLVMSVDRLD YSKGLVERFR AFEKLLEHET SFRNHVSFLQ IAPSTRADLR AYQDIRRQLE GESGRINGRF AELDWAPILY IHRQYERPVL AALYRLARVG YVTPLRDGMN LVAKEYVSAQ DPDDPGVLVL SRFAGAACEL TGALIVNPID IAGMADALSR ALSMPLAERR ARHADMLARL RENNVSVWRD NFLRDLGR
NT Sequence
ATGAGCCGTC TCATCATCGT CTCGAATCGG GTCGCGCCGA TTTCGGAAGG GGAGCCCGCC GCGGGCGGTC TCGCGATCGG TGTCTACGAC GCGCTGAAGG AAACGGGCGG CACGTGGTTC GGCTGGAGCG GCGAGGTTGT CGCGTCGGGC GCGCCGCAGA TTCGCGTCGA GGAGCACGGC CCCGTCACGT TCGCGACGAT CGGGCTGTCG CGCCGCGATT ACGATCAGTA CTACCGAGGC TTCTCGAACG CGACGCTGTG GCCGGCGTTC CATTACCGCG CGGACCTGAT TCAGTTCGAT CGCCACGAGT TCGAGGGCTA TTCGCGCGTG AACGTGTGGC TCGCGCGGCA GCTCGTGCCG CTGTTGCGCG ACGACGACGT GATCTGGGTG CACGACTATC ACCTGATTCC GTTCGCGCAG GCGCTGCGCG CGGCCGGCGT GAAGAACCGG ATCGGCTTCT TCCTGCACAT TCCGTTTCCG GCCGCGCAGG TGCTCGTCAA CGTGCCGCCG CATCGGGAGC TTGTCGAGGC GCTGTGCTCG TTCGATCTGC TCGGCTTCCA GACCGACACC GATCTGCGCG CGTTCTTCGA ATACGTCGAA TGCGAGGCGG GCGGCGCGGT CGACCGCGCC GCGCACCCGG CGCGCGTCGA GGCGTTCGGG CGCACGCTGC GCGCGGCCGC GTATCCGATC GGCGTCTATC CGGACGAGAT CGCCGCGCTC GCGAAGGCGG GCGAGAACGG CCGCGCGGTG AAGGCGCTCG CGGCGTCGCT GCGCGGGCGG CAGCTCGTGA TGAGCGTCGA CCGGCTCGAT TACTCGAAGG GGCTCGTCGA GCGCTTTCGC GCGTTCGAGA AGCTGCTCGA GCACGAGACG TCGTTTCGCA ATCACGTGTC GTTCCTGCAG ATCGCGCCGT CGACGCGCGC CGACCTGCGC GCGTACCAGG ACATCCGCCG TCAGCTCGAA GGCGAGTCGG GGCGCATCAA TGGGCGCTTC GCGGAGCTCG ACTGGGCGCC GATCCTCTAC ATCCATCGGC AGTACGAGCG GCCGGTGCTG GCCGCGCTGT ACCGGCTCGC GCGTGTCGGC TACGTGACGC CGCTGCGCGA CGGGATGAAT CTCGTCGCGA AGGAGTATGT GTCCGCGCAG GACCCGGACG ATCCCGGCGT GCTGGTGCTG TCGCGCTTCG CGGGCGCGGC GTGCGAGCTT ACGGGCGCGC TGATCGTCAA TCCGATCGAC ATCGCCGGGA TGGCCGACGC GTTGTCGCGC GCGCTGTCGA TGCCGCTCGC CGAGCGGCGC GCGCGTCACG CCGACATGCT CGCGCGGCTG CGCGAGAACA ACGTGTCGGT GTGGCGCGAC AACTTCTTGC GGGATCTCGG GCGA
Details for ButhA.00046.a.B1.GE36044
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 52kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 24
PERCENT COVERAGE: 72
Validated AA Sequence
MDVEDRRPVE LREAPIDAPR LAFELTADVL VRAQVGARRR RDLQERHVIA KRRLVLEQLL ERAKALDEPL RVIEPVDAHH ELPPAQRRRE RLHRAAVLAR LRERGDLVRI DADRIRGRAQ RAPERLDARR VRGAVDRAAR LAFDVFEERA QIGVGLEAEQ IERAQRLDKL PMRRHVDEHL RGRKRNVQEE ADPVLHAGRA QRLRERNQVI VVHPDHVVVA QQRHELPREP HVHARIALEL VAIELNQVRA VMERRPQRRV REASVVLIVI AARQPDRRER DGAVLLDANL RRARRDNLAA PAEPRAARFL QRVVDTDRET ARGGLPFRNR RDPIRDDDE
Validated NT Sequence
cgcgccacac cgacacgttg ttctcgcgca gccgcgcgag catgtcggcg tgacgcgcgc gccgctcggc gagcggcatc gacagcgcgc gcgacaacgc gtcggccatc ccggcgatgt cgatcggatt gacgatcagc gcgcccgtaa gctcgcacgc cgcgcccgcg aagcgcgaca gcaccagcac gccgggatcg tccgggtcct gcgcggacac atactccttc gcgacgagat tcatcccgtc gcgcagcggc gtcacgtagc cgacacgcgc gagccggtac agcgcggcca gcaccggccg ctcgtactgc cgatggatgt agaggatcgg cgcccagtcg agctccgcga agcgcccatt gatgcgcccc gactcgcctt cgagctgacg gcggatgtcc tggtacgcgc gcaggtcggc gcgcgtcgac ggcgcgatct gcaggaacga cacgtgattg cgaaacgacg tctcgtgctc gagcagcttc tcgaacgcgc gaaagcgctc gacgagcccc ttcgagtaat cgagccggtc gacgctcatc acgagctgcc gcccgcgcag cgacgccgcg agcgccttca ccgcgcggcc gttctcgccc gccttcgcga gcgcggcgat ctcgtccgga tagacgccga tcggatacgc ggccgcgcgc agcgtgcgcc cgaacgcctc gacgcgcgcc gggtgcgcgg cgcggtcgac cgcgccgccc gcctcgcatt cgacgtattc gaagaacgcg cgcagatcgg tgtcggtctg gaagccgagc agatcgaacg agcacagcgc ctcgacaagc tcccgatgcg gcggcacgtt gacgagcacc tgcgcggccg gaaacggaat gtgcaggaag aagccgatcc ggttcttcac gccggccgcg cgcagcgcct gcgcgaacgg aatcaggtga tagtcgtgca cccagatcac gtcgtcgtcg cgcaacagcg gcacgagctg ccgcgcgagc cacacgttca cgcgcgaata gccctcgaac tcgtggcgat cgaactgaat caggtccgcg cggtaatgga acgccggcca cagcgtcgcg ttcgagaagc ctcggtagta ctgatcgtaa tcgcggcgcg acagcccgat cgtcgcgaac gtgacggggc cgtgctcctc gacgcgaatc tgcggcgcgc ccgacgcgac aacctcgccg ctccagccga accacgtgcc gcccgtttcc ttcagcgcgt cgtagacacc gatcgcgaga ccgcccgcgg cgggctcccc ttccgaaatc ggcgcgaccc gattcgagac gatgatgaga c
Expected Protein Sequence
MAHHHHHHMS RLIIVSNRVA PISEGEPAAG GLAIGVYDAL KETGGTWFGW SGEVVASGAP QIRVEEHGPV TFATIGLSRR DYDQYYRGFS NATLWPAFHY RADLIQFDRH EFEGYSRVNV WLARQLVPLL RDDDVIWVHD YHLIPFAQAL RAAGVKNRIG FFLHIPFPAA QVLVNVPPHR ELVEALCSFD LLGFQTDTDL RAFFEYVECE AGGAVDRAAH PARVEAFGRT LRAAAYPIGV YPDEIAALAK AGENGRAVKA LAASLRGRQL VMSVDRLDYS KGLVERFRAF EKLLEHETSF RNHVSFLQIA PSTRADLRAY QDIRRQLEGE SGRINGRFAE LDWAPILYIH RQYERPVLAA LYRLARVGYV TPLRDGMNLV AKEYVSAQDP DDPGVLVLSR FAGAACELTG ALIVNPIDIA GMADALSRAL SMPLAERRAR HADMLARLRE NNVSVWRDNF LRDLGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc gtctcatcat cgtctcgaat cgggtcgcgc cgatttcgga aggggagccc gccgcgggcg gtctcgcgat cggtgtctac gacgcgctga aggaaacggg cggcacgtgg ttcggctgga gcggcgaggt tgtcgcgtcg ggcgcgccgc agattcgcgt cgaggagcac ggccccgtca cgttcgcgac gatcgggctg tcgcgccgcg attacgatca gtactaccga ggcttctcga acgcgacgct gtggccggcg ttccattacc gcgcggacct gattcagttc gatcgccacg agttcgaggg ctattcgcgc gtgaacgtgt ggctcgcgcg gcagctcgtg ccgctgttgc gcgacgacga cgtgatctgg gtgcacgact atcacctgat tccgttcgcg caggcgctgc gcgcggccgg cgtgaagaac cggatcggct tcttcctgca cattccgttt ccggccgcgc aggtgctcgt caacgtgccg ccgcatcggg agcttgtcga ggcgctgtgc tcgttcgatc tgctcggctt ccagaccgac accgatctgc gcgcgttctt cgaatacgtc gaatgcgagg cgggcggcgc ggtcgaccgc gccgcgcacc cggcgcgcgt cgaggcgttc gggcgcacgc tgcgcgcggc cgcgtatccg atcggcgtct atccggacga gatcgccgcg ctcgcgaagg cgggcgagaa cggccgcgcg gtgaaggcgc tcgcggcgtc gctgcgcggg cggcagctcg tgatgagcgt cgaccggctc gattactcga aggggctcgt cgagcgcttt cgcgcgttcg agaagctgct cgagcacgag acgtcgtttc gcaatcacgt gtcgttcctg cagatcgcgc cgtcgacgcg cgccgacctg cgcgcgtacc aggacatccg ccgtcagctc gaaggcgagt cggggcgcat caatgggcgc ttcgcggagc tcgactgggc gccgatcctc tacatccatc ggcagtacga gcggccggtg ctggccgcgc tgtaccggct cgcgcgtgtc ggctacgtga cgccgctgcg cgacgggatg aatctcgtcg cgaaggagta tgtgtccgcg caggacccgg acgatcccgg cgtgctggtg ctgtcgcgct tcgcgggcgc ggcgtgcgag cttacgggcg cgctgatcgt caatccgatc gacatcgccg ggatggccga cgcgttgtcg cgcgcgctgt cgatgccgct cgccgagcgg cgcgcgcgtc acgccgacat gctcgcgcgg ctgcgcgaga acaacgtgtc ggtgtggcgc gacaacttct tgcgggatct cgggcgatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at