ButhA.00039.b

Methionine aminopeptidase

CENTER ID: ButhA.00039.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00039.b.B1.GE36043 Full length( ButhA.00039.b ) 1 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00039.b.B1.PS01740 Full length( ButhA.00039.b ) 1 275

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4783
RefSeq: YP_442502.1
UniProt: Q2SX47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARSRHVPIR TQAEIAMARR AGTMAARVLG MIAPHVKAGV TTNELDRLCR EYIVDILRAR PANIGYHGYP KTICASVNHV VCHGIPSDHA LKDGDIINID VALDSDGWYG DTSRMYFVGE PAEPARRLVD ATYEAMMAGV AAVRPGATLG DVGHAIQSVA HREGFSVVRE YCGHGIGRIY HDEPQVLHYG RPGTGLRLAP GMIFTIEPML NAGRAETRQL ADGWTVVTQD RSWSAQWEHM VVVTEDGFDI LTPWPDAAPE RSPVTARAAG APERG
NT Sequence
ATGGCGCGTT CACGACACGT TCCGATCCGC ACGCAAGCCG AGATCGCGAT GGCGCGCCGC GCCGGCACGA TGGCGGCGCG GGTGTTGGGC ATGATCGCGC CGCACGTGAA GGCTGGCGTG ACGACGAACG AGCTCGACCG GCTATGCCGC GAGTACATCG TCGACATCCT GCGCGCGCGG CCCGCGAACA TCGGCTATCA CGGGTATCCG AAGACGATCT GCGCATCCGT GAACCACGTG GTCTGCCACG GCATTCCGTC CGATCACGCA TTGAAGGACG GCGACATCAT CAATATCGAC GTCGCGCTCG ACAGCGACGG CTGGTACGGC GACACGAGCC GCATGTACTT CGTCGGCGAG CCGGCTGAGC CGGCTCGGCG TCTCGTCGAT GCGACGTATG AGGCGATGAT GGCGGGCGTC GCGGCGGTGC GCCCGGGCGC GACGCTCGGC GACGTCGGCC ACGCGATCCA GAGCGTCGCG CATCGCGAGG GCTTCAGCGT CGTACGCGAG TATTGCGGCC ACGGCATCGG CCGCATCTAC CACGACGAGC CGCAAGTGCT CCATTACGGC CGTCCGGGCA CGGGGCTGCG TCTCGCGCCC GGGATGATCT TCACGATCGA GCCGATGCTC AATGCGGGGC GCGCCGAAAC GCGGCAGCTC GCCGACGGCT GGACGGTCGT CACGCAGGAT CGCTCGTGGT CCGCGCAGTG GGAGCATATG GTCGTCGTGA CGGAAGACGG CTTCGACATC CTGACGCCGT GGCCGGACGC AGCGCCCGAG CGGTCGCCCG TGACGGCGCG CGCGGCCGGC GCGCCCGAGC GCGGC
Details for ButhA.00039.b.B1.GE36043
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RSRHVPIRTQ AEIAMARRAG TMAARVLGMI APHVKAGVTT NELDRLCREY IVDILRARPA NIGYHGYPKT ICASVNHVVC HGIPSDHALK DGDIINIDVA LDSDGWYGDT SRMYFVGEPA EPARRLVDAT YEAMMAGVAA VRPGATLGDV GHAIQSVAHR EGFSVVREYC GHGIGRIYHD EPQVLHYGRP GTGLRLAPGM IFTIEPMLNA GRAETRQLAD GWTVVTQDRS WSAQWEHMVV VTEDGFDILT PWPDAAPERS PVTARAAGAP ERG
Validated NT Sequence
atcttactca cttagccgcg ctcgggcgcg ccggccgcgc gcgccgtcac gggcgaccgc tcgggcgctg cgtccggcca cggcgtcagg atgtcgaagc cgtcttccgt cacgacgacc atatgctccc actgcgcgga ccacgagcga tcctgcgtga cgaccgtcca gccgtcggcg agctgccgcg tttcggcgcg ccccgcattg agcatcggct cgatcgtgaa gatcatcccg ggcgcgagac gcagccccgt gcccggacgg ccgtaatgga gcacttgcgg ctcgtcgtgg tagatgcggc cgatgccgtg gccgcaatac tcgcgtacga cgctgaagcc ctcgcgatgc gcgacgctct ggatcgcgtg gccgacgtcg ccgagcgtcg cgcccgggcg caccgccgcg acgcccgcca tcatcgcctc atacgtcgca tcgacgagac gccgagccgg ctcagccggc tcgccgacga agtacatgcg gctcgtgtcg ccgtaccagc cgtcgctgtc gagcgcgacg tcgatattga tgatgtcgcc gtccttcaat gcgtgatcgg acggaatgcc gtggcagacc acgtggttca cggatgcgca gatcgtcttc ggatacccgt gatagccgat gttcgcgggc cgcgcgcgca ggatgtcgac gatgtactcg cggcatagcc ggtcgagctc gttcgtcgtc acgccagcct tcacgtgcgg cgcgatcatg cccaacaccc gcgccgccat cgtgccggcg cggcgcgcca tcgcgatctc ggcttgcgtg cggatcggaa cgtgtcgtga acgcgccata tggtggtggt ggtggtgagc catggtat
Expected Protein Sequence
MAHHHHHHMA RSRHVPIRTQ AEIAMARRAG TMAARVLGMI APHVKAGVTT NELDRLCREY IVDILRARPA NIGYHGYPKT ICASVNHVVC HGIPSDHALK DGDIINIDVA LDSDGWYGDT SRMYFVGEPA EPARRLVDAT YEAMMAGVAA VRPGATLGDV GHAIQSVAHR EGFSVVREYC GHGIGRIYHD EPQVLHYGRP GTGLRLAPGM IFTIEPMLNA GRAETRQLAD GWTVVTQDRS WSAQWEHMVV VTEDGFDILT PWPDAAPERS PVTARAAGAP ERG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gttcacgaca cgttccgatc cgcacgcaag ccgagatcgc gatggcgcgc cgcgccggca cgatggcggc gcgggtgttg ggcatgatcg cgccgcacgt gaaggctggc gtgacgacga acgagctcga ccggctatgc cgcgagtaca tcgtcgacat cctgcgcgcg cggcccgcga acatcggcta tcacgggtat ccgaagacga tctgcgcatc cgtgaaccac gtggtctgcc acggcattcc gtccgatcac gcattgaagg acggcgacat catcaatatc gacgtcgcgc tcgacagcga cggctggtac ggcgacacga gccgcatgta cttcgtcggc gagccggctg agccggctcg gcgtctcgtc gatgcgacgt atgaggcgat gatggcgggc gtcgcggcgg tgcgcccggg cgcgacgctc ggcgacgtcg gccacgcgat ccagagcgtc gcgcatcgcg agggcttcag cgtcgtacgc gagtattgcg gccacggcat cggccgcatc taccacgacg agccgcaagt gctccattac ggccgtccgg gcacggggct gcgtctcgcg cccgggatga tcttcacgat cgagccgatg ctcaatgcgg ggcgcgccga aacgcggcag ctcgccgacg gctggacggt cgtcacgcag gatcgctcgt ggtccgcgca gtgggagcat atggtcgtcg tgacggaaga cggcttcgac atcctgacgc cgtggccgga cgcagcgccc gagcggtcgc ccgtgacggc gcgcgcggcc ggcgcgcccg agcgcggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ButhA.00039.b.B1.PS01740
PURIFICATION DATe: 2/5/2013
CONCENTRATION: 17mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RSRHVPIRTQ AEIAMARRAG TMAARVLGMI APHVKAGVTT NELDRLCREY IVDILRARPA NIGYHGYPKT ICASVNHVVC HGIPSDHALK DGDIINIDVA LDSDGWYGDT SRMYFVGEPA EPARRLVDAT YEAMMAGVAA VRPGATLGDV GHAIQSVAHR EGFSVVREYC GHGIGRIYHD EPQVLHYGRP GTGLRLAPGM IFTIEPMLNA GRAETRQLAD GWTVVTQDRS WSAQWEHMVV VTEDGFDILT PWPDAAPERS PVTARAAGAP ERG
Validated NT Sequence
atcttactca cttagccgcg ctcgggcgcg ccggccgcgc gcgccgtcac gggcgaccgc tcgggcgctg cgtccggcca cggcgtcagg atgtcgaagc cgtcttccgt cacgacgacc atatgctccc actgcgcgga ccacgagcga tcctgcgtga cgaccgtcca gccgtcggcg agctgccgcg tttcggcgcg ccccgcattg agcatcggct cgatcgtgaa gatcatcccg ggcgcgagac gcagccccgt gcccggacgg ccgtaatgga gcacttgcgg ctcgtcgtgg tagatgcggc cgatgccgtg gccgcaatac tcgcgtacga cgctgaagcc ctcgcgatgc gcgacgctct ggatcgcgtg gccgacgtcg ccgagcgtcg cgcccgggcg caccgccgcg acgcccgcca tcatcgcctc atacgtcgca tcgacgagac gccgagccgg ctcagccggc tcgccgacga agtacatgcg gctcgtgtcg ccgtaccagc cgtcgctgtc gagcgcgacg tcgatattga tgatgtcgcc gtccttcaat gcgtgatcgg acggaatgcc gtggcagacc acgtggttca cggatgcgca gatcgtcttc ggatacccgt gatagccgat gttcgcgggc cgcgcgcgca ggatgtcgac gatgtactcg cggcatagcc ggtcgagctc gttcgtcgtc acgccagcct tcacgtgcgg cgcgatcatg cccaacaccc gcgccgccat cgtgccggcg cggcgcgcca tcgcgatctc ggcttgcgtg cggatcggaa cgtgtcgtga acgcgccata tggtggtggt ggtggtgagc catggtat
Expressed Protein Sequence
MAHHHHHHMA RSRHVPIRTQ AEIAMARRAG TMAARVLGMI APHVKAGVTT NELDRLCREY IVDILRARPA NIGYHGYPKT ICASVNHVVC HGIPSDHALK DGDIINIDVA LDSDGWYGDT SRMYFVGEPA EPARRLVDAT YEAMMAGVAA VRPGATLGDV GHAIQSVAHR EGFSVVREYC GHGIGRIYHD EPQVLHYGRP GTGLRLAPGM IFTIEPMLNA GRAETRQLAD GWTVVTQDRS WSAQWEHMVV VTEDGFDILT PWPDAAPERS PVTARAAGAP ERG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGC GTTCACGACA CGTTCCGATC CGCACGCAAG CCGAGATCGC GATGGCGCGC CGCGCCGGCA CGATGGCGGC GCGGGTGTTG GGCATGATCG CGCCGCACGT GAAGGCTGGC GTGACGACGA ACGAGCTCGA CCGGCTATGC CGCGAGTACA TCGTCGACAT CCTGCGCGCG CGGCCCGCGA ACATCGGCTA TCACGGGTAT CCGAAGACGA TCTGCGCATC CGTGAACCAC GTGGTCTGCC ACGGCATTCC GTCCGATCAC GCATTGAAGG ACGGCGACAT CATCAATATC GACGTCGCGC TCGACAGCGA CGGCTGGTAC GGCGACACGA GCCGCATGTA CTTCGTCGGC GAGCCGGCTG AGCCGGCTCG GCGTCTCGTC GATGCGACGT ATGAGGCGAT GATGGCGGGC GTCGCGGCGG TGCGCCCGGG CGCGACGCTC GGCGACGTCG GCCACGCGAT CCAGAGCGTC GCGCATCGCG AGGGCTTCAG CGTCGTACGC GAGTATTGCG GCCACGGCAT CGGCCGCATC TACCACGACG AGCCGCAAGT GCTCCATTAC GGCCGTCCGG GCACGGGGCT GCGTCTCGCG CCCGGGATGA TCTTCACGAT CGAGCCGATG CTCAATGCGG GGCGCGCCGA AACGCGGCAG CTCGCCGACG GCTGGACGGT CGTCACGCAG GATCGCTCGT GGTCCGCGCA GTGGGAGCAT ATGGTCGTCG TGACGGAAGA CGGCTTCGAC ATCCTGACGC CGTGGCCGGA CGCAGCGCCC GAGCGGTCGC CCGTGACGGC GCGCGCGGCC GGCGCGCCCG AGCGCGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT