ButhA.00027.g

Acyl-CoA dehydrogenase

CENTER ID: ButhA.00027.g
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00027.g.B1.GE36039 Full length( ButhA.00027.g ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.328
RefSeq: YP_438493.1
UniProt: Q2T8K6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHCTTPNPAY YRPEHIELQD TLRRFVAREI SPYVNDWDEA GEFPRELYEK AARIGLLGIG FPDAYGGVED VDMFHRLICS IELARCGAGG VVSSLMSHTI GAPPIVHAGS DELKARVLPG ILSGRKISAL AITEPGGGSD VAALRASAIR DGDHYVVNGE KVFITSGIRA DYYTVAVRTD PHARGAKGLS LLLVEGDTAG LSKTPLKKMG WWASDTAHLR FDDCRVPAAN RIGPEGGGFP LVMQNFNLER FSLAASALGF AQVCYDDALD WARQRRTFGQ RVADHQVIRH TLVDMAMRIG ATRAWLEQCA WQMDHADGRA PALVATICML KNFATQTMQF CADAAVQILG GMGYMRGTRP ERIYREVKVN MIGGGAQEIM KDLAARQLEY
NT Sequence
ATGCATTGCA CGACCCCGAA CCCGGCGTAC TACCGGCCCG AACACATCGA ATTGCAGGAC ACGCTGCGCC GGTTCGTCGC GAGAGAGATC TCGCCTTACG TGAACGACTG GGACGAGGCG GGCGAGTTTC CGCGCGAGCT GTACGAGAAG GCGGCGCGAA TCGGGCTGCT CGGCATCGGC TTTCCGGACG CGTACGGCGG AGTCGAAGAC GTCGACATGT TTCATCGGCT GATCTGCTCG ATCGAACTCG CGCGCTGCGG CGCGGGCGGC GTCGTCAGCT CGCTGATGTC GCACACGATC GGTGCGCCGC CGATCGTGCA TGCGGGCAGC GACGAGCTGA AGGCGCGCGT GCTGCCCGGC ATTCTCTCGG GCCGCAAGAT CTCGGCGCTC GCGATCACGG AACCGGGCGG CGGATCGGAC GTCGCCGCGC TGCGAGCGAG CGCGATTCGC GACGGCGATC ACTATGTCGT GAACGGCGAG AAGGTGTTCA TCACGTCGGG CATCCGCGCC GACTACTACA CGGTAGCCGT GCGCACCGAT CCGCATGCGC GGGGCGCGAA GGGGCTGTCG CTGCTGCTCG TCGAGGGCGA CACGGCGGGC CTGTCGAAGA CGCCGCTGAA GAAGATGGGC TGGTGGGCGT CCGACACCGC GCATCTGCGC TTCGACGATT GCCGCGTGCC GGCCGCGAAC CGGATCGGCC CGGAGGGCGG CGGCTTTCCG CTCGTCATGC AGAACTTCAA TCTCGAGCGC TTCAGCCTGG CCGCGTCGGC GCTCGGTTTC GCGCAAGTCT GCTACGACGA CGCGCTCGAC TGGGCGCGGC AGCGCAGGAC GTTCGGGCAG CGCGTGGCCG ATCATCAGGT GATTCGGCAC ACGCTCGTCG ACATGGCGAT GCGCATCGGC GCGACGCGCG CGTGGCTCGA GCAATGCGCG TGGCAGATGG ATCACGCCGA CGGGCGCGCG CCGGCGCTCG TCGCGACGAT CTGCATGCTG AAGAACTTCG CTACGCAGAC GATGCAGTTC TGCGCGGACG CCGCGGTGCA GATCCTCGGC GGCATGGGCT ACATGCGCGG CACGCGGCCC GAGCGCATCT ACCGCGAGGT GAAGGTCAAC ATGATCGGCG GCGGCGCGCA GGAGATCATG AAGGATCTCG CCGCGCGGCA GCTCGAGTAT
Details for ButhA.00027.g.B1.GE36039
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 43kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMH CTTPNPAYYR PEHIELQDTL RRFVAREISP YVNDWDEAGE FPRELYEKAA RIGLLGIGFP DAYGGVEDVD MFHRLICSIE LARCGAGGVV SSLMSHTIGA PPIVHAGSDE LKARVLPGIL SGRKISALAI TEPGGGSDVA ALRASAIRDG DHYVVNGEKV FITSGIRADY YTVAVRTDPH ARGAKGLSLL LVEGDTAGLS KTPLKKMGWW ASDTAHLRFD DCRVPAANRI GPEGGGFPLV MQNFNLERFS LAASALGFAQ VCYDDALDWA RQRXTFGQRV ADHXXFGTRS STWRCXSARR ARGSSXXXXX WITPXXXXXR SSXXXXXXXX XYXXXAXXXX XXXXILXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttgntnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgcattgc acgaccccga acccggcgta ctaccggccc gaacacatcg aattgcagga cacgctgcgc cggttcgtcg cgagagagat ctcgccttac gtgaacgact gggacgaggc gggcgagttt ccgcgcgagc tgtacgagaa ggcggcgcga atcgggctgc tcggcatcgg ctttccggac gcgtacggcg gagtcgaaga cgtcgacatg tttcatcggc tgatctgctc gatcgaactc gcgcgctgcg gcgcgggcgg cgtcgtcagc tcgctgatgt cgcacacgat cggtgcgccg ccgatcgtgc atgcgggcag cgacgagctg aaggcgcgcg tgctgcccgg cattctctcg ggccgcaaga tctcggcgct cgcgatcacg gaaccgggcg gcggatcgga cgtcgccgcg ctgcgagcga gcgcgattcg cgacggcgat cactatgtcg tgaacggcga gaaggtgttc atcacgtcgg gcatccgcgc cgactactac acggtagccg tgcgcaccga tccgcatgcg cggggcgcga aggggctgtc gctgctgctc gtcgagggcg acacggcggg cctgtcgaag acgccgctga agaagatggg ctggtgggcg tccgacaccg cgcatctgcg cttcgacgat tgccgcgtgc cggccgcgaa ccggatcggc ccggagggcg gcggctttcc gctcgtcatg cagaacttca atctcgagcg cttcagcctg gccgcgtcgg cgctcggttt cgcgcaagtc tgctacgacg acgcgctcga ctgggcgcgg cagcgcnnga cgttcgggca gcgcgtggcc gatcatcnnn gattcggcac acgctcgtcg acatggcgat gcncatcggc gcgacgcgcg cgtggctcga gcnntgnncn nngnngatgg atcacgccga nnnnnnnncn nnnncgctcg tcnngacnan cnncatgnnn annantnnnc tacnnnnann angcantncn ncnnnnnncc nnnnngnnna tcctcnnnnn ntnnnntnnn tnnnnnnnnn nnnnnnnann nannnncnnn ngnnnnannn nnnnnnnnnn nnnngnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMH CTTPNPAYYR PEHIELQDTL RRFVAREISP YVNDWDEAGE FPRELYEKAA RIGLLGIGFP DAYGGVEDVD MFHRLICSIE LARCGAGGVV SSLMSHTIGA PPIVHAGSDE LKARVLPGIL SGRKISALAI TEPGGGSDVA ALRASAIRDG DHYVVNGEKV FITSGIRADY YTVAVRTDPH ARGAKGLSLL LVEGDTAGLS KTPLKKMGWW ASDTAHLRFD DCRVPAANRI GPEGGGFPLV MQNFNLERFS LAASALGFAQ VCYDDALDWA RQRRTFGQRV ADHQVIRHTL VDMAMRIGAT RAWLEQCAWQ MDHADGRAPA LVATICMLKN FATQTMQFCA DAAVQILGGM GYMRGTRPER IYREVKVNMI GGGAQEIMKD LAARQLEY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatt gcacgacccc gaacccggcg tactaccggc ccgaacacat cgaattgcag gacacgctgc gccggttcgt cgcgagagag atctcgcctt acgtgaacga ctgggacgag gcgggcgagt ttccgcgcga gctgtacgag aaggcggcgc gaatcgggct gctcggcatc ggctttccgg acgcgtacgg cggagtcgaa gacgtcgaca tgtttcatcg gctgatctgc tcgatcgaac tcgcgcgctg cggcgcgggc ggcgtcgtca gctcgctgat gtcgcacacg atcggtgcgc cgccgatcgt gcatgcgggc agcgacgagc tgaaggcgcg cgtgctgccc ggcattctct cgggccgcaa gatctcggcg ctcgcgatca cggaaccggg cggcggatcg gacgtcgccg cgctgcgagc gagcgcgatt cgcgacggcg atcactatgt cgtgaacggc gagaaggtgt tcatcacgtc gggcatccgc gccgactact acacggtagc cgtgcgcacc gatccgcatg cgcggggcgc gaaggggctg tcgctgctgc tcgtcgaggg cgacacggcg ggcctgtcga agacgccgct gaagaagatg ggctggtggg cgtccgacac cgcgcatctg cgcttcgacg attgccgcgt gccggccgcg aaccggatcg gcccggaggg cggcggcttt ccgctcgtca tgcagaactt caatctcgag cgcttcagcc tggccgcgtc ggcgctcggt ttcgcgcaag tctgctacga cgacgcgctc gactgggcgc ggcagcgcag gacgttcggg cagcgcgtgg ccgatcatca ggtgattcgg cacacgctcg tcgacatggc gatgcgcatc ggcgcgacgc gcgcgtggct cgagcaatgc gcgtggcaga tggatcacgc cgacgggcgc gcgccggcgc tcgtcgcgac gatctgcatg ctgaagaact tcgctacgca gacgatgcag ttctgcgcgg acgccgcggt gcagatcctc ggcggcatgg gctacatgcg cggcacgcgg cccgagcgca tctaccgcga ggtgaaggtc aacatgatcg gcggcggcgc gcaggagatc atgaaggatc tcgccgcgcg gcagctcgag tattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat