ButhA.00027.e
Acyl-CoA dehydrogenase
| CENTER ID: | ButhA.00027.e |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00027.e.B1.GE36037 | Full length( ButhA.00027.e ) | 1 | 377 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00027.e.B1.PW37239 | Full length( ButhA.00027.e ) | 1 | 377 |
Structures
4M9A
DEPOSITED: 8/14/2013
DETERMINATION: XRay
CLONE: ButhA.00027.e.B1.GE36037
PROTEIN: ButhA.00027.e.B1.PW37239
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127618 |
| PATRIC ID: | fig|271848.6.peg.2009 |
| RefSeq: | YP_439997.1 |
| UniProt: | Q2T4A2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDLYTEDQR MILDAARAFC AEVLAPNAAQ WDRESHLPDE VVAQMGELGF LGMIVPADWG GSYTDYVAYA LALEEIAAGC ASCATLVSVH NSVGCGPVLN YGTTEQKERW LRDLASGKTV GAFSLTEPHA GSEAHNLRTR AELRDGKWIL NGSKQFVTNG ARAGLAIVFA MTDPDEGKRG LSAFVVPTDT PGFIVGKPEK KMGIRASDTC PITLENCAIP QENLLGKRGE GLKIALSNLE GGRIGIAAQA TGIARAAFDR ARRYARERVQ FGKPIAEHQA IAEKLANMAT QINAARLLTH HAARLRTAGL PCLSEASQAK LFASEMAEAV CSDAIQIHGG YGFLVDYEVE RHYRDARITQ IYEGTSEVQR MVIARQL
NT Sequence
ATGGACGATC TCTACACCGA AGATCAACGG ATGATCCTCG ACGCCGCGCG CGCCTTCTGC GCCGAGGTGC TCGCGCCGAA CGCCGCCCAG TGGGACCGCG AATCGCATCT GCCCGACGAG GTCGTCGCGC AGATGGGCGA GCTCGGTTTC CTCGGCATGA TCGTGCCCGC CGACTGGGGC GGATCGTATA CGGATTACGT CGCCTACGCG CTCGCGCTCG AGGAGATCGC GGCGGGCTGC GCGTCGTGCG CGACGCTCGT GAGCGTGCAC AACTCGGTCG GCTGCGGCCC GGTGCTGAAC TACGGCACGA CCGAGCAAAA GGAGCGCTGG CTGCGCGATC TCGCGAGCGG CAAGACGGTT GGCGCGTTCA GCCTCACCGA GCCGCACGCG GGCTCCGAGG CGCACAACCT GCGCACCCGC GCGGAGCTGC GCGACGGCAA GTGGATTCTG AACGGCAGCA AGCAGTTCGT CACGAACGGC GCGCGCGCGG GCCTGGCGAT CGTTTTTGCC ATGACCGATC CGGACGAGGG CAAGCGCGGG CTGTCCGCGT TCGTCGTGCC GACCGATACG CCGGGCTTCA TCGTCGGCAA GCCCGAGAAG AAGATGGGCA TTCGCGCGTC GGACACGTGC CCGATCACCC TCGAGAACTG CGCGATTCCC CAGGAGAATC TGCTCGGCAA GCGCGGCGAG GGGCTGAAGA TCGCACTGTC GAATCTCGAG GGCGGGCGCA TCGGCATCGC CGCGCAGGCG ACGGGCATCG CGCGCGCCGC GTTCGACCGC GCGCGCCGCT ATGCGCGCGA GCGCGTCCAG TTCGGCAAGC CGATCGCCGA GCATCAGGCG ATCGCCGAGA AGCTCGCGAA CATGGCGACG CAGATCAACG CCGCGCGCCT GCTCACGCAT CACGCGGCGC GGCTTCGCAC GGCGGGGCTG CCGTGCCTGT CGGAGGCGTC GCAGGCGAAG CTCTTCGCGT CCGAGATGGC CGAGGCGGTG TGCTCGGATG CGATCCAGAT TCATGGCGGC TACGGTTTCC TCGTCGATTA CGAGGTCGAG CGCCATTACC GCGATGCGCG GATCACGCAG ATCTACGAGG GGACCAGCGA AGTGCAAAGA ATGGTGATCG CGCGGCAGCT G
Details for ButhA.00027.e.B1.GE36037
| HARVESTED ON: | 10/2/2012 |
| SEQUENCED ON: | 10/1/2012 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 83 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMD DLYTEDQRMI LDAARAFCAE VLAPNAAQWD RESHLPDEVV AQMGELGFLG MIVPADWGGS YTDYVAYALA LEEIAAGCAS CATLVSVHNS VGCGPVLNYG TTEQKERWLR DLASGKTVGA FSLTEPHAGS EAHNLRTRAE LRDGKWILNG SKQFVTNGAR AGLAIVFAMT DPDEGKRGLS AFVVPTDTPG FIVGKPEKKM GIRASDTCPI TLENCAIPQE NLLGKRGEGL KIALSNLEGG RIGIAAXATG IARAAFDRAR RYARERVQFG KPIAEHXXIA EXLANMATXI NAARLLXHHA ARLRTAGXPX LXXXXXXXXX XREXXLXXXX XXGXTXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatggac gatctctaca ccgaagatca acggatgatc ctcgacgccg cgcgcgcctt ctgcgccgag gtgctcgcgc cgaacgccgc ccagtgggac cgcgaatcgc atctgcccga cgaggtcgtc gcgcagatgg gcgagctcgg tttcctcggc atgatcgtgc ccgccgactg gggcggatcg tatacggatt acgtcgccta cgcgctcgcg ctcgaggaga tcgcggcggg ctgcgcgtcg tgcgcgacgc tcgtgagcgt gcacaactcg gtcggctgcg gcccggtgct gaactacggc acgaccgagc aaaaggagcg ctggctgcgc gatctcgcga gcggcaagac ggttggcgcg ttcagcctca ccgagccgca cgcgggctcc gaggcgcaca acctgcgcac ccgcgcggag ctgcgcgacg gcaagtggat tctgaacggc agcaagcagt tcgtcacgaa cggcgcgcgc gcgggcctgg cgatcgtttt tgccatgacc gatccggacg agggcaagcg cgggctgtcc gcgttcgtcg tgccgaccga tacgccgggc ttcatcgtcg gcaagcccga gaagaagatg ggcattcgcg cgtcggacac gtgcccgatc accctcgaga actgcgcgat tccccaggag aatctgctcg gcaagcgcgg cgaggggctg aagatcgcac tgtcgaatct cgagggcggg cgcatcggca tcgccgcgca ngcgacgggc atcgcgcgcg ccgcgttcga ccgcgcgcgc cgctatgcgc gcgagcgcgt ccagttcggc aagccgatcg ccgagcatcn nncgatcgcc gagangctcg cgaacatggc gacgcanatc aacgccgcgc gcctgctcnc gcatcacgcg gcgcggcttc gcacggcggg gntgccgngc ctgncnnnnn nnncgcnnnn ancnnntnnc nnncgagagn nnnnnctnnn nngcnatnca natnntggnn nnacnntnnn nnncnannnn nnnnnnnnnn nnnnnnnnnn nnnnnnngnn cnnnnnnann ntgnnnnnnn nannnnnann nnnnnnatnn ncnnnnnnnn nnt
Expected Protein Sequence
MAHHHHHHMD DLYTEDQRMI LDAARAFCAE VLAPNAAQWD RESHLPDEVV AQMGELGFLG MIVPADWGGS YTDYVAYALA LEEIAAGCAS CATLVSVHNS VGCGPVLNYG TTEQKERWLR DLASGKTVGA FSLTEPHAGS EAHNLRTRAE LRDGKWILNG SKQFVTNGAR AGLAIVFAMT DPDEGKRGLS AFVVPTDTPG FIVGKPEKKM GIRASDTCPI TLENCAIPQE NLLGKRGEGL KIALSNLEGG RIGIAAQATG IARAAFDRAR RYARERVQFG KPIAEHQAIA EKLANMATQI NAARLLTHHA ARLRTAGLPC LSEASQAKLF ASEMAEAVCS DAIQIHGGYG FLVDYEVERH YRDARITQIY EGTSEVQRMV IARQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg atctctacac cgaagatcaa cggatgatcc tcgacgccgc gcgcgccttc tgcgccgagg tgctcgcgcc gaacgccgcc cagtgggacc gcgaatcgca tctgcccgac gaggtcgtcg cgcagatggg cgagctcggt ttcctcggca tgatcgtgcc cgccgactgg ggcggatcgt atacggatta cgtcgcctac gcgctcgcgc tcgaggagat cgcggcgggc tgcgcgtcgt gcgcgacgct cgtgagcgtg cacaactcgg tcggctgcgg cccggtgctg aactacggca cgaccgagca aaaggagcgc tggctgcgcg atctcgcgag cggcaagacg gttggcgcgt tcagcctcac cgagccgcac gcgggctccg aggcgcacaa cctgcgcacc cgcgcggagc tgcgcgacgg caagtggatt ctgaacggca gcaagcagtt cgtcacgaac ggcgcgcgcg cgggcctggc gatcgttttt gccatgaccg atccggacga gggcaagcgc gggctgtccg cgttcgtcgt gccgaccgat acgccgggct tcatcgtcgg caagcccgag aagaagatgg gcattcgcgc gtcggacacg tgcccgatca ccctcgagaa ctgcgcgatt ccccaggaga atctgctcgg caagcgcggc gaggggctga agatcgcact gtcgaatctc gagggcgggc gcatcggcat cgccgcgcag gcgacgggca tcgcgcgcgc cgcgttcgac cgcgcgcgcc gctatgcgcg cgagcgcgtc cagttcggca agccgatcgc cgagcatcag gcgatcgccg agaagctcgc gaacatggcg acgcagatca acgccgcgcg cctgctcacg catcacgcgg cgcggcttcg cacggcgggg ctgccgtgcc tgtcggaggc gtcgcaggcg aagctcttcg cgtccgagat ggccgaggcg gtgtgctcgg atgcgatcca gattcatggc ggctacggtt tcctcgtcga ttacgaggtc gagcgccatt accgcgatgc gcggatcacg cagatctacg aggggaccag cgaagtgcaa agaatggtga tcgcgcggca gctgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for ButhA.00027.e.B1.PW37239
| PURIFICATION DATe: | 2/4/2012 |
| CONCENTRATION: | 30.6mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 83 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD DLYTEDQRMI LDAARAFCAE VLAPNAAQWD RESHLPDEVV AQMGELGFLG MIVPADWGGS YTDYVAYALA LEEIAAGCAS CATLVSVHNS VGCGPVLNYG TTEQKERWLR DLASGKTVGA FSLTEPHAGS EAHNLRTRAE LRDGKWILNG SKQFVTNGAR AGLAIVFAMT DPDEGKRGLS AFVVPTDTPG FIVGKPEKKM GIRASDTCPI TLENCAIPQE NLLGKRGEGL KIALSNLEGG RIGIAAXATG IARAAFDRAR RYARERVQFG KPIAEHXXIA EXLANMATXI NAARLLXHHA ARLRTAGXPX LXXXXXXXXX XREXXLXXXX XXGXTXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatggac gatctctaca ccgaagatca acggatgatc ctcgacgccg cgcgcgcctt ctgcgccgag gtgctcgcgc cgaacgccgc ccagtgggac cgcgaatcgc atctgcccga cgaggtcgtc gcgcagatgg gcgagctcgg tttcctcggc atgatcgtgc ccgccgactg gggcggatcg tatacggatt acgtcgccta cgcgctcgcg ctcgaggaga tcgcggcggg ctgcgcgtcg tgcgcgacgc tcgtgagcgt gcacaactcg gtcggctgcg gcccggtgct gaactacggc acgaccgagc aaaaggagcg ctggctgcgc gatctcgcga gcggcaagac ggttggcgcg ttcagcctca ccgagccgca cgcgggctcc gaggcgcaca acctgcgcac ccgcgcggag ctgcgcgacg gcaagtggat tctgaacggc agcaagcagt tcgtcacgaa cggcgcgcgc gcgggcctgg cgatcgtttt tgccatgacc gatccggacg agggcaagcg cgggctgtcc gcgttcgtcg tgccgaccga tacgccgggc ttcatcgtcg gcaagcccga gaagaagatg ggcattcgcg cgtcggacac gtgcccgatc accctcgaga actgcgcgat tccccaggag aatctgctcg gcaagcgcgg cgaggggctg aagatcgcac tgtcgaatct cgagggcggg cgcatcggca tcgccgcgca ngcgacgggc atcgcgcgcg ccgcgttcga ccgcgcgcgc cgctatgcgc gcgagcgcgt ccagttcggc aagccgatcg ccgagcatcn nncgatcgcc gagangctcg cgaacatggc gacgcanatc aacgccgcgc gcctgctcnc gcatcacgcg gcgcggcttc gcacggcggg gntgccgngc ctgncnnnnn nnncgcnnnn ancnnntnnc nnncgagagn nnnnnctnnn nngcnatnca natnntggnn nnacnntnnn nnncnannnn nnnnnnnnnn nnnnnnnnnn nnnnnnngnn cnnnnnnann ntgnnnnnnn nannnnnann nnnnnnatnn ncnnnnnnnn nnt
Expressed Protein Sequence
MAHHHHHHMD DLYTEDQRMI LDAARAFCAE VLAPNAAQWD RESHLPDEVV AQMGELGFLG MIVPADWGGS YTDYVAYALA LEEIAAGCAS CATLVSVHNS VGCGPVLNYG TTEQKERWLR DLASGKTVGA FSLTEPHAGS EAHNLRTRAE LRDGKWILNG SKQFVTNGAR AGLAIVFAMT DPDEGKRGLS AFVVPTDTPG FIVGKPEKKM GIRASDTCPI TLENCAIPQE NLLGKRGEGL KIALSNLEGG RIGIAAQATG IARAAFDRAR RYARERVQFG KPIAEHQAIA EKLANMATQI NAARLLTHHA ARLRTAGLPC LSEASQAKLF ASEMAEAVCS DAIQIHGGYG FLVDYEVERH YRDARITQIY EGTSEVQRMV IARQL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG ATCTCTACAC CGAAGATCAA CGGATGATCC TCGACGCCGC GCGCGCCTTC TGCGCCGAGG TGCTCGCGCC GAACGCCGCC CAGTGGGACC GCGAATCGCA TCTGCCCGAC GAGGTCGTCG CGCAGATGGG CGAGCTCGGT TTCCTCGGCA TGATCGTGCC CGCCGACTGG GGCGGATCGT ATACGGATTA CGTCGCCTAC GCGCTCGCGC TCGAGGAGAT CGCGGCGGGC TGCGCGTCGT GCGCGACGCT CGTGAGCGTG CACAACTCGG TCGGCTGCGG CCCGGTGCTG AACTACGGCA CGACCGAGCA AAAGGAGCGC TGGCTGCGCG ATCTCGCGAG CGGCAAGACG GTTGGCGCGT TCAGCCTCAC CGAGCCGCAC GCGGGCTCCG AGGCGCACAA CCTGCGCACC CGCGCGGAGC TGCGCGACGG CAAGTGGATT CTGAACGGCA GCAAGCAGTT CGTCACGAAC GGCGCGCGCG CGGGCCTGGC GATCGTTTTT GCCATGACCG ATCCGGACGA GGGCAAGCGC GGGCTGTCCG CGTTCGTCGT GCCGACCGAT ACGCCGGGCT TCATCGTCGG CAAGCCCGAG AAGAAGATGG GCATTCGCGC GTCGGACACG TGCCCGATCA CCCTCGAGAA CTGCGCGATT CCCCAGGAGA ATCTGCTCGG CAAGCGCGGC GAGGGGCTGA AGATCGCACT GTCGAATCTC GAGGGCGGGC GCATCGGCAT CGCCGCGCAG GCGACGGGCA TCGCGCGCGC CGCGTTCGAC CGCGCGCGCC GCTATGCGCG CGAGCGCGTC CAGTTCGGCA AGCCGATCGC CGAGCATCAG GCGATCGCCG AGAAGCTCGC GAACATGGCG ACGCAGATCA ACGCCGCGCG CCTGCTCACG CATCACGCGG CGCGGCTTCG CACGGCGGGG CTGCCGTGCC TGTCGGAGGC GTCGCAGGCG AAGCTCTTCG CGTCCGAGAT GGCCGAGGCG GTGTGCTCGG ATGCGATCCA GATTCATGGC GGCTACGGTT TCCTCGTCGA TTACGAGGTC GAGCGCCATT ACCGCGATGC GCGGATCACG CAGATCTACG AGGGGACCAG CGAAGTGCAA AGAATGGTGA TCGCGCGGCA GCTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT