SSGCID: Target Page

ButhA.00027.d

Acyl-CoA dehydrogenase

CENTER ID:	ButhA.00027.d
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00027.d.B1.GE36036	Full length( ButhA.00027.d )		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00027.d.B1.PW36681	Full length( ButhA.00027.d )		1	378

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2712
RefSeq:	YP_440620.1
UniProt:	Q2T2J3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIPRTIFAEE HEQFRESVRR FIESEVMPYH ERWEEQGYVD RGVWTKAAEA GYHCASMPEA YGGAGADIRY SIVLFEEIAR AGASGLGFGL HSEIVAPYIL HYGDDALKAR YLPKLASAEM IGAIAMTEPG AGSDLQGVRT SAVRDGDHYV LNGSKIFITN GWHADVVIVV ARTTPEGGSK GTSLFVVDTG MAGFSKGKRL KKVGMKAQDT SELFFDGVRV PAGNLLGEEN RGFVYLMQEL PWERLQIAIS AIASAEAALA WTLDYTRDRR AFGRAVIDFQ TSRHALAELK SEIQIGRVFV DKCIELQLAG KLDAATASMA KYWTTELQFK VIDRCVQLHG GYGYMWEYPI ARAWADSRVQ QIYGGTNEIM KELIARTL

NT Sequence

ATGATTCCCC GCACCATCTT CGCCGAAGAG CACGAGCAGT TCCGCGAGTC CGTCCGCCGT TTCATCGAAT CCGAGGTGAT GCCGTACCAC GAGCGCTGGG AGGAACAGGG CTACGTCGAT CGCGGCGTGT GGACGAAGGC GGCCGAGGCG GGCTACCACT GCGCGAGCAT GCCCGAAGCG TACGGCGGCG CGGGCGCGGA CATCCGCTAC AGCATCGTGC TGTTCGAGGA GATCGCGCGC GCGGGCGCGT CGGGGCTAGG CTTCGGGCTG CATTCGGAGA TCGTCGCGCC GTACATCCTC CATTACGGCG ACGATGCGCT CAAGGCGCGC TACCTGCCGA AGCTCGCGAG CGCCGAGATG ATCGGCGCGA TCGCGATGAC CGAGCCCGGC GCGGGCTCCG ACCTGCAAGG CGTGCGCACG AGCGCGGTGC GCGACGGCGA TCACTACGTG CTCAACGGCT CGAAGATCTT CATCACGAAC GGCTGGCACG CGGACGTCGT GATCGTCGTC GCGCGCACGA CGCCCGAAGG CGGCTCGAAG GGCACGAGCC TGTTCGTCGT CGATACGGGC ATGGCGGGCT TCAGCAAGGG CAAGCGGCTG AAAAAGGTGG GCATGAAGGC GCAGGACACG TCGGAGCTCT TTTTCGACGG CGTGCGCGTG CCCGCTGGCA ATCTGCTCGG CGAGGAGAAC CGCGGCTTCG TCTATCTGAT GCAGGAGCTG CCGTGGGAGC GGCTGCAGAT CGCGATCAGC GCGATCGCGT CGGCCGAGGC GGCGCTTGCG TGGACGCTCG ACTACACGCG CGACCGGCGC GCGTTCGGCC GTGCGGTGAT CGACTTCCAG ACGAGCCGCC ACGCGCTCGC CGAGCTGAAG AGCGAGATCC AGATCGGCCG CGTGTTCGTC GACAAATGCA TCGAGCTGCA GCTCGCGGGC AAGCTCGATG CGGCCACGGC CTCGATGGCG AAGTACTGGA CGACCGAGCT GCAGTTCAAG GTGATCGATC GCTGCGTGCA GCTGCACGGC GGCTACGGCT ACATGTGGGA ATACCCGATC GCGCGCGCGT GGGCCGATTC GCGCGTGCAG CAGATCTACG GCGGCACCAA TGAAATCATG AAGGAGCTGA TCGCGCGCAC GCTG

Details for ButhA.00027.d.B1.GE36036

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	74

Validated AA Sequence

MPYHERWEEQ GYVDRGVWTK AAEAGYHCAS MPEAYGGAGA DIRYSIVLFE EIARAGASGL GFGLHSEIVA PYILHYGDDA LKARYLPKLA SAEMIGAIAM TEPGAGSDLQ GVRTSAVRDG DHYVLNGSKI FITNGWHADV VIVVARTTPE GGSKGTSLFV VDTGMAGFSK GKRLKKVGMK AQDTSELFFD GVRVPAGNLL GEENRGFVYL MQELPWERLQ IAISAIASAE AALAWTLDYT RDRRAFGRAV IDFQTSRHAL AELKSEIQIG RVFVDKCIEL QLAGKLDAA

Validated NT Sequence

gtggccgcat cgagcttgcc cgcgagctgc agctcgatgc atttgtcgac gaacacgcgg ccgatctgga tctcgctctt cagctcggcg agcgcgtggc ggctcgtctg gaagtcgatc accgcacggc cgaacgcgcg ccggtcgcgc gtgtagtcga gcgtccacgc aagcgccgcc tcggccgacg cgatcgcgct gatcgcgatc tgcagccgct cccacggcag ctcctgcatc agatagacga agccgcggtt ctcctcgccg agcagattgc cagcgggcac gcgcacgccg tcgaaaaaga gctccgacgt gtcctgcgcc ttcatgccca cctttttcag ccgcttgccc ttgctgaagc ccgccatgcc cgtatcgacg acgaacaggc tcgtgccctt cgagccgcct tcgggcgtcg tgcgcgcgac gacgatcacg acgtccgcgt gccagccgtt cgtgatgaag atcttcgagc cgttgagcac gtagtgatcg ccgtcgcgca ccgcgctcgt gcgcacgcct tgcaggtcgg agcccgcgcc gggctcggtc atcgcgatcg cgccgatcat ctcggcgctc gcgagcttcg gcaggtagcg cgccttgagc gcatcgtcgc cgtaatggag gatgtacggc gcgacgatct ccgaatgcag cccgaagcct agccccgacg cgcccgcgcg cgcgatctcc tcgaacagca cgatgctgta gcggatgtcc gcgcccgcgc cgccgtacgc ttcgggcatg ctcgcgcagt ggtagcccgc ctcggccgcc ttcgtccaca cgccgcgatc gacgtagccc tgttcctccc agcgctcgtg gtacggcatc acctcggatt cgatgaaacg gcggacggac tcgcggaact gctcgtgctc ttcggcgaag atggtgcggg g

Expected Protein Sequence

MAHHHHHHMI PRTIFAEEHE QFRESVRRFI ESEVMPYHER WEEQGYVDRG VWTKAAEAGY HCASMPEAYG GAGADIRYSI VLFEEIARAG ASGLGFGLHS EIVAPYILHY GDDALKARYL PKLASAEMIG AIAMTEPGAG SDLQGVRTSA VRDGDHYVLN GSKIFITNGW HADVVIVVAR TTPEGGSKGT SLFVVDTGMA GFSKGKRLKK VGMKAQDTSE LFFDGVRVPA GNLLGEENRG FVYLMQELPW ERLQIAISAI ASAEAALAWT LDYTRDRRAF GRAVIDFQTS RHALAELKSE IQIGRVFVDK CIELQLAGKL DAATASMAKY WTTELQFKVI DRCVQLHGGY GYMWEYPIAR AWADSRVQQI YGGTNEIMKE LIARTL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc cccgcaccat cttcgccgaa gagcacgagc agttccgcga gtccgtccgc cgtttcatcg aatccgaggt gatgccgtac cacgagcgct gggaggaaca gggctacgtc gatcgcggcg tgtggacgaa ggcggccgag gcgggctacc actgcgcgag catgcccgaa gcgtacggcg gcgcgggcgc ggacatccgc tacagcatcg tgctgttcga ggagatcgcg cgcgcgggcg cgtcggggct aggcttcggg ctgcattcgg agatcgtcgc gccgtacatc ctccattacg gcgacgatgc gctcaaggcg cgctacctgc cgaagctcgc gagcgccgag atgatcggcg cgatcgcgat gaccgagccc ggcgcgggct ccgacctgca aggcgtgcgc acgagcgcgg tgcgcgacgg cgatcactac gtgctcaacg gctcgaagat cttcatcacg aacggctggc acgcggacgt cgtgatcgtc gtcgcgcgca cgacgcccga aggcggctcg aagggcacga gcctgttcgt cgtcgatacg ggcatggcgg gcttcagcaa gggcaagcgg ctgaaaaagg tgggcatgaa ggcgcaggac acgtcggagc tctttttcga cggcgtgcgc gtgcccgctg gcaatctgct cggcgaggag aaccgcggct tcgtctatct gatgcaggag ctgccgtggg agcggctgca gatcgcgatc agcgcgatcg cgtcggccga ggcggcgctt gcgtggacgc tcgactacac gcgcgaccgg cgcgcgttcg gccgtgcggt gatcgacttc cagacgagcc gccacgcgct cgccgagctg aagagcgaga tccagatcgg ccgcgtgttc gtcgacaaat gcatcgagct gcagctcgcg ggcaagctcg atgcggccac ggcctcgatg gcgaagtact ggacgaccga gctgcagttc aaggtgatcg atcgctgcgt gcagctgcac ggcggctacg gctacatgtg ggaatacccg atcgcgcgcg cgtgggccga ttcgcgcgtg cagcagatct acggcggcac caatgaaatc atgaaggagc tgatcgcgcg cacgctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for ButhA.00027.d.B1.PW36681

PURIFICATION DATe:	12/31/2012
CONCENTRATION:	33.7mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	74

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTC CCCGCACCAT CTTCGCCGAA GAGCACGAGC AGTTCCGCGA GTCCGTCCGC CGTTTCATCG AATCCGAGGT GATGCCGTAC CACGAGCGCT GGGAGGAACA GGGCTACGTC GATCGCGGCG TGTGGACGAA GGCGGCCGAG GCGGGCTACC ACTGCGCGAG CATGCCCGAA GCGTACGGCG GCGCGGGCGC GGACATCCGC TACAGCATCG TGCTGTTCGA GGAGATCGCG CGCGCGGGCG CGTCGGGGCT AGGCTTCGGG CTGCATTCGG AGATCGTCGC GCCGTACATC CTCCATTACG GCGACGATGC GCTCAAGGCG CGCTACCTGC CGAAGCTCGC GAGCGCCGAG ATGATCGGCG CGATCGCGAT GACCGAGCCC GGCGCGGGCT CCGACCTGCA AGGCGTGCGC ACGAGCGCGG TGCGCGACGG CGATCACTAC GTGCTCAACG GCTCGAAGAT CTTCATCACG AACGGCTGGC ACGCGGACGT CGTGATCGTC GTCGCGCGCA CGACGCCCGA AGGCGGCTCG AAGGGCACGA GCCTGTTCGT CGTCGATACG GGCATGGCGG GCTTCAGCAA GGGCAAGCGG CTGAAAAAGG TGGGCATGAA GGCGCAGGAC ACGTCGGAGC TCTTTTTCGA CGGCGTGCGC GTGCCCGCTG GCAATCTGCT CGGCGAGGAG AACCGCGGCT TCGTCTATCT GATGCAGGAG CTGCCGTGGG AGCGGCTGCA GATCGCGATC AGCGCGATCG CGTCGGCCGA GGCGGCGCTT GCGTGGACGC TCGACTACAC GCGCGACCGG CGCGCGTTCG GCCGTGCGGT GATCGACTTC CAGACGAGCC GCCACGCGCT CGCCGAGCTG AAGAGCGAGA TCCAGATCGG CCGCGTGTTC GTCGACAAAT GCATCGAGCT GCAGCTCGCG GGCAAGCTCG ATGCGGCCAC GGCCTCGATG GCGAAGTACT GGACGACCGA GCTGCAGTTC AAGGTGATCG ATCGCTGCGT GCAGCTGCAC GGCGGCTACG GCTACATGTG GGAATACCCG ATCGCGCGCG CGTGGGCCGA TTCGCGCGTG CAGCAGATCT ACGGCGGCAC CAATGAAATC ATGAAGGAGC TGATCGCGCG CACGCTGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT