ButhA.00027.c

Acyl-CoA dehydrogenase domain protein

CENTER ID: ButhA.00027.c
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00027.c.B1.GE36035 Full length( ButhA.00027.c ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00027.c.B1.PS01747 Full length( ButhA.00027.c ) 1 403

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3065
RefSeq: YP_440952.1
UniProt: Q2T1J7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLNFTAEEE AFRAQVQRFL ADELPPRISR KVKGGLRLTR DDMREWHAIL NARGWLASHW PREWGGPGWS VAQKFLFDNE CAIAGAPRIV PFGVNMLGPV LIRYGSEAQK RRWLPRILDG TDWWCQGYSE PGAGSDLAAV KTSAVRGVDA QGDHYVVNGQ KTWTTLGHYA NMIFCLVRTA TDVRKQEGIS FLLVDMNAPG VEVRPIVTLD GEHEVNEVFF TDVRVPAENL VGEENRGWTY AKYLLTYERT NIAGVGFSVA ALDRLRAVAA KVKRNGRPLA DDPLFAARLA RVEIELDNMK TTNLRVLAAV AGGGAPGAES SMLKIRGTQI RQEISSLMRR AMGPYAQPFV DEALDADYDG EPLGGLPEAA SAATQYFNNR KLSIFGGSNE IQKNIISKTM LGL
NT Sequence
ATGGATCTCA ATTTCACCGC TGAAGAGGAA GCGTTCCGCG CGCAGGTGCA GCGCTTTCTC GCCGACGAGC TGCCGCCGCG CATTTCGCGC AAGGTGAAGG GCGGCTTGCG GCTCACGCGC GACGACATGC GCGAATGGCA CGCCATCCTC AACGCGCGGG GCTGGCTCGC GAGCCACTGG CCGCGCGAAT GGGGCGGCCC CGGCTGGAGC GTCGCGCAGA AGTTCCTGTT CGACAACGAG TGCGCGATCG CGGGCGCGCC GCGCATCGTG CCGTTCGGCG TGAACATGCT CGGCCCCGTG CTGATCAGGT ACGGCAGCGA AGCGCAGAAG CGCCGCTGGC TGCCGCGCAT TCTCGACGGC ACCGACTGGT GGTGCCAGGG CTATTCGGAG CCGGGCGCGG GCTCCGATCT CGCCGCGGTG AAAACGAGCG CGGTGCGGGG CGTCGACGCG CAGGGCGACC ACTACGTCGT CAACGGGCAG AAGACGTGGA CGACGCTCGG GCACTACGCG AACATGATCT TCTGCCTCGT GCGCACCGCG ACCGACGTGC GCAAGCAGGA AGGCATCAGC TTCCTGCTCG TCGACATGAA CGCGCCCGGC GTCGAGGTGC GCCCGATCGT CACGCTCGAC GGCGAGCACG AAGTCAACGA AGTGTTCTTC ACCGACGTGC GCGTGCCGGC CGAAAACCTC GTCGGCGAAG AGAACCGCGG CTGGACCTAC GCGAAATACC TGCTCACGTA CGAGCGCACG AACATCGCGG GCGTGGGCTT CTCGGTCGCG GCGCTGGACA GGCTGCGCGC GGTCGCGGCG AAGGTGAAGA GGAACGGCCG GCCGCTCGCC GACGATCCGC TCTTCGCCGC GCGGCTCGCT CGCGTCGAGA TCGAGCTCGA CAACATGAAG ACGACGAACC TGCGCGTGCT CGCGGCCGTC GCGGGCGGCG GCGCGCCGGG CGCGGAGAGC TCGATGCTGA AGATTCGCGG CACGCAGATC CGCCAGGAGA TTTCGTCGCT GATGCGTCGC GCGATGGGGC CGTATGCGCA GCCGTTCGTC GACGAGGCGC TCGACGCCGA TTACGACGGC GAGCCGTTGG GCGGCCTGCC CGAGGCGGCG AGCGCCGCGA CGCAATACTT CAACAACCGC AAGCTGTCGA TCTTCGGCGG CTCGAACGAG ATCCAGAAGA ACATCATCTC GAAGACGATG CTCGGGTTG
Details for ButhA.00027.c.B1.GE36035
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Validated AA Sequence
MREWHAILNA RGWLASHWPR EWGGPGWSVA QKFLFDNECA IAGAPRIVPF GVNMLGPVLI RYGSEAQKRR WLPRILDGTD WWCQGYSEPG AGSDLAAVKT SAVRGVDAQG DHYVVNGQKT WTTLGHYANM IFCLVRTATD VRKQEGISFL LVDMNAPGVE VRPIVTLDGE HEVNEVFFTD VRVPAENLVG EENRGWTYAK YLLTYERTNI AGVGFSVAAL DRLRAVAAKV KRNGRPLADD PLFAARLARV EIELDNMKTT NLRVLAAVAG GGAPGAESSM LKIRGTQIRQ EISSLMRRAM GPYAQPFVDE ALDADYDGEP LGGLPEAASA ATQYFNNRKL SIFGGSNEIQ KN
Validated NT Sequence
atgttcttct ggatctcgtt cgagccgccg aagatcgaca gcttgcggtt gttgaagtat tgcgtcgcgg cgctcgccgc ctcgggcagg ccgcccaacg gctcgccgtc gtaatcggcg tcgagcgcct cgtcgacgaa cggctgcgca tacggcccca tcgcgcgacg catcagcgac gaaatctcct ggcggatctg cgtgccgcga atcttcagca tcgagctctc cgcgcccggc gcgccgccgc ccgcgacggc cgcgagcacg cgcaggttcg tcgtcttcat gttgtcgagc tcgatctcga cgcgagcgag ccgcgcggcg aagagcggat cgtcggcgag cggccggccg ttcctcttca ccttcgccgc gaccgcgcgc agcctgtcca gcgccgcgac cgagaagccc acgcccgcga tgttcgtgcg ctcgtacgtg agcaggtatt tcgcgtaggt ccagccgcgg ttctcttcgc cgacgaggtt ttcggccggc acgcgcacgt cggtgaagaa cacttcgttg acttcgtgct cgccgtcgag cgtgacgatc gggcgcacct cgacgccggg cgcgttcatg tcgacgagca ggaagctgat gccttcctgc ttgcgcacgt cggtcgcggt gcgcacgagg cagaagatca tgttcgcgta gtgcccgagc gtcgtccacg tcttctgccc gttgacgacg tagtggtcgc cctgcgcgtc gacgccccgc accgcgctcg ttttcaccgc ggcgagatcg gagcccgcgc ccggctccga atagccctgg caccaccagt cggtgccgtc gagaatgcgc ggcagccagc ggcgcttctg cgcttcgctg ccgtacctga tcagcacggg gccgagcatg ttcacgccga acggcacgat gcgcggcgcg cccgcgatcg cgcactcgtt gtcgaacagg aacttctgcg cgacgctcca gccggggccg ccccattcgc gcggccagtg gctcgcgagc cagccccgcg cgttgaggat ggcgtgccat tcgcgcatgt cgtcgcgcgt gagccgcaag ccgcccttca ccttgcgcga aatgcgcggc ggcagctcgt cggcgagaaa gcgctgcacc tgcgcgcgga acgcttcctc ttcagcggtg aaatt
Expected Protein Sequence
MAHHHHHHMD LNFTAEEEAF RAQVQRFLAD ELPPRISRKV KGGLRLTRDD MREWHAILNA RGWLASHWPR EWGGPGWSVA QKFLFDNECA IAGAPRIVPF GVNMLGPVLI RYGSEAQKRR WLPRILDGTD WWCQGYSEPG AGSDLAAVKT SAVRGVDAQG DHYVVNGQKT WTTLGHYANM IFCLVRTATD VRKQEGISFL LVDMNAPGVE VRPIVTLDGE HEVNEVFFTD VRVPAENLVG EENRGWTYAK YLLTYERTNI AGVGFSVAAL DRLRAVAAKV KRNGRPLADD PLFAARLARV EIELDNMKTT NLRVLAAVAG GGAPGAESSM LKIRGTQIRQ EISSLMRRAM GPYAQPFVDE ALDADYDGEP LGGLPEAASA ATQYFNNRKL SIFGGSNEIQ KNIISKTMLG L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tcaatttcac cgctgaagag gaagcgttcc gcgcgcaggt gcagcgcttt ctcgccgacg agctgccgcc gcgcatttcg cgcaaggtga agggcggctt gcggctcacg cgcgacgaca tgcgcgaatg gcacgccatc ctcaacgcgc ggggctggct cgcgagccac tggccgcgcg aatggggcgg ccccggctgg agcgtcgcgc agaagttcct gttcgacaac gagtgcgcga tcgcgggcgc gccgcgcatc gtgccgttcg gcgtgaacat gctcggcccc gtgctgatca ggtacggcag cgaagcgcag aagcgccgct ggctgccgcg cattctcgac ggcaccgact ggtggtgcca gggctattcg gagccgggcg cgggctccga tctcgccgcg gtgaaaacga gcgcggtgcg gggcgtcgac gcgcagggcg accactacgt cgtcaacggg cagaagacgt ggacgacgct cgggcactac gcgaacatga tcttctgcct cgtgcgcacc gcgaccgacg tgcgcaagca ggaaggcatc agcttcctgc tcgtcgacat gaacgcgccc ggcgtcgagg tgcgcccgat cgtcacgctc gacggcgagc acgaagtcaa cgaagtgttc ttcaccgacg tgcgcgtgcc ggccgaaaac ctcgtcggcg aagagaaccg cggctggacc tacgcgaaat acctgctcac gtacgagcgc acgaacatcg cgggcgtggg cttctcggtc gcggcgctgg acaggctgcg cgcggtcgcg gcgaaggtga agaggaacgg ccggccgctc gccgacgatc cgctcttcgc cgcgcggctc gctcgcgtcg agatcgagct cgacaacatg aagacgacga acctgcgcgt gctcgcggcc gtcgcgggcg gcggcgcgcc gggcgcggag agctcgatgc tgaagattcg cggcacgcag atccgccagg agatttcgtc gctgatgcgt cgcgcgatgg ggccgtatgc gcagccgttc gtcgacgagg cgctcgacgc cgattacgac ggcgagccgt tgggcggcct gcccgaggcg gcgagcgccg cgacgcaata cttcaacaac cgcaagctgt cgatcttcgg cggctcgaac gagatccaga agaacatcat ctcgaagacg atgctcgggt tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ButhA.00027.c.B1.PS01747
PURIFICATION DATe: 2/14/2013
CONCENTRATION: 5.3mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MREWHAILNA RGWLASHWPR EWGGPGWSVA QKFLFDNECA IAGAPRIVPF GVNMLGPVLI RYGSEAQKRR WLPRILDGTD WWCQGYSEPG AGSDLAAVKT SAVRGVDAQG DHYVVNGQKT WTTLGHYANM IFCLVRTATD VRKQEGISFL LVDMNAPGVE VRPIVTLDGE HEVNEVFFTD VRVPAENLVG EENRGWTYAK YLLTYERTNI AGVGFSVAAL DRLRAVAAKV KRNGRPLADD PLFAARLARV EIELDNMKTT NLRVLAAVAG GGAPGAESSM LKIRGTQIRQ EISSLMRRAM GPYAQPFVDE ALDADYDGEP LGGLPEAASA ATQYFNNRKL SIFGGSNEIQ KN
Validated NT Sequence
atgttcttct ggatctcgtt cgagccgccg aagatcgaca gcttgcggtt gttgaagtat tgcgtcgcgg cgctcgccgc ctcgggcagg ccgcccaacg gctcgccgtc gtaatcggcg tcgagcgcct cgtcgacgaa cggctgcgca tacggcccca tcgcgcgacg catcagcgac gaaatctcct ggcggatctg cgtgccgcga atcttcagca tcgagctctc cgcgcccggc gcgccgccgc ccgcgacggc cgcgagcacg cgcaggttcg tcgtcttcat gttgtcgagc tcgatctcga cgcgagcgag ccgcgcggcg aagagcggat cgtcggcgag cggccggccg ttcctcttca ccttcgccgc gaccgcgcgc agcctgtcca gcgccgcgac cgagaagccc acgcccgcga tgttcgtgcg ctcgtacgtg agcaggtatt tcgcgtaggt ccagccgcgg ttctcttcgc cgacgaggtt ttcggccggc acgcgcacgt cggtgaagaa cacttcgttg acttcgtgct cgccgtcgag cgtgacgatc gggcgcacct cgacgccggg cgcgttcatg tcgacgagca ggaagctgat gccttcctgc ttgcgcacgt cggtcgcggt gcgcacgagg cagaagatca tgttcgcgta gtgcccgagc gtcgtccacg tcttctgccc gttgacgacg tagtggtcgc cctgcgcgtc gacgccccgc accgcgctcg ttttcaccgc ggcgagatcg gagcccgcgc ccggctccga atagccctgg caccaccagt cggtgccgtc gagaatgcgc ggcagccagc ggcgcttctg cgcttcgctg ccgtacctga tcagcacggg gccgagcatg ttcacgccga acggcacgat gcgcggcgcg cccgcgatcg cgcactcgtt gtcgaacagg aacttctgcg cgacgctcca gccggggccg ccccattcgc gcggccagtg gctcgcgagc cagccccgcg cgttgaggat ggcgtgccat tcgcgcatgt cgtcgcgcgt gagccgcaag ccgcccttca ccttgcgcga aatgcgcggc ggcagctcgt cggcgagaaa gcgctgcacc tgcgcgcgga acgcttcctc ttcagcggtg aaatt
Expressed Protein Sequence
MAHHHHHHMD LNFTAEEEAF RAQVQRFLAD ELPPRISRKV KGGLRLTRDD MREWHAILNA RGWLASHWPR EWGGPGWSVA QKFLFDNECA IAGAPRIVPF GVNMLGPVLI RYGSEAQKRR WLPRILDGTD WWCQGYSEPG AGSDLAAVKT SAVRGVDAQG DHYVVNGQKT WTTLGHYANM IFCLVRTATD VRKQEGISFL LVDMNAPGVE VRPIVTLDGE HEVNEVFFTD VRVPAENLVG EENRGWTYAK YLLTYERTNI AGVGFSVAAL DRLRAVAAKV KRNGRPLADD PLFAARLARV EIELDNMKTT NLRVLAAVAG GGAPGAESSM LKIRGTQIRQ EISSLMRRAM GPYAQPFVDE ALDADYDGEP LGGLPEAASA ATQYFNNRKL SIFGGSNEIQ KNIISKTMLG L
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC TCAATTTCAC CGCTGAAGAG GAAGCGTTCC GCGCGCAGGT GCAGCGCTTT CTCGCCGACG AGCTGCCGCC GCGCATTTCG CGCAAGGTGA AGGGCGGCTT GCGGCTCACG CGCGACGACA TGCGCGAATG GCACGCCATC CTCAACGCGC GGGGCTGGCT CGCGAGCCAC TGGCCGCGCG AATGGGGCGG CCCCGGCTGG AGCGTCGCGC AGAAGTTCCT GTTCGACAAC GAGTGCGCGA TCGCGGGCGC GCCGCGCATC GTGCCGTTCG GCGTGAACAT GCTCGGCCCC GTGCTGATCA GGTACGGCAG CGAAGCGCAG AAGCGCCGCT GGCTGCCGCG CATTCTCGAC GGCACCGACT GGTGGTGCCA GGGCTATTCG GAGCCGGGCG CGGGCTCCGA TCTCGCCGCG GTGAAAACGA GCGCGGTGCG GGGCGTCGAC GCGCAGGGCG ACCACTACGT CGTCAACGGG CAGAAGACGT GGACGACGCT CGGGCACTAC GCGAACATGA TCTTCTGCCT CGTGCGCACC GCGACCGACG TGCGCAAGCA GGAAGGCATC AGCTTCCTGC TCGTCGACAT GAACGCGCCC GGCGTCGAGG TGCGCCCGAT CGTCACGCTC GACGGCGAGC ACGAAGTCAA CGAAGTGTTC TTCACCGACG TGCGCGTGCC GGCCGAAAAC CTCGTCGGCG AAGAGAACCG CGGCTGGACC TACGCGAAAT ACCTGCTCAC GTACGAGCGC ACGAACATCG CGGGCGTGGG CTTCTCGGTC GCGGCGCTGG ACAGGCTGCG CGCGGTCGCG GCGAAGGTGA AGAGGAACGG CCGGCCGCTC GCCGACGATC CGCTCTTCGC CGCGCGGCTC GCTCGCGTCG AGATCGAGCT CGACAACATG AAGACGACGA ACCTGCGCGT GCTCGCGGCC GTCGCGGGCG GCGGCGCGCC GGGCGCGGAG AGCTCGATGC TGAAGATTCG CGGCACGCAG ATCCGCCAGG AGATTTCGTC GCTGATGCGT CGCGCGATGG GGCCGTATGC GCAGCCGTTC GTCGACGAGG CGCTCGACGC CGATTACGAC GGCGAGCCGT TGGGCGGCCT GCCCGAGGCG GCGAGCGCCG CGACGCAATA CTTCAACAAC CGCAAGCTGT CGATCTTCGG CGGCTCGAAC GAGATCCAGA AGAACATCAT CTCGAAGACG ATGCTCGGGT TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT