ButhA.00027.b

Acyl-CoA dehydrogenase domain protein

CENTER ID: ButhA.00027.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00027.b.B1.GE36034 Full length( ButhA.00027.b ) 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3066
RefSeq: YP_440953.1
UniProt: Q2T1J6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFRYTEDRR MLADSLNRFI AEQYPFAARD RIALSEPGYS DDMWRRFAEL GAIGALFPED CGGLGGAGFD IALVFECLGR GLVVEPFAGA LMTGRALAQA GGDAHRARLA DLIEGRAIGA FAHAEPGTHY EPYTVRTRAA REGGRWVLDG AKAVVDCGEH ATFFVVSART AGADDAAAGL SLFVVPKTAP GVTARGYRKI DGGRAADVRF ERVALADDAL VGAPGEAADA IERALGFGLL ALAAEALGAM DVAKAYTLEY LRTRRQFGVP IGSFQALQHR MAELLLEIEQ ARSAVINAAA QLDAPRVARE RALSAAKYSV GRIGALVAEE SIQLHGGIGM TWELPLAHYA KRLVMIDHQL GDEDHHLARY VALSKQ
NT Sequence
ATGGACTTTC GATACACCGA AGACCGCCGG ATGCTGGCGG ACAGCCTGAA CCGCTTCATC GCCGAGCAGT ACCCGTTCGC CGCGCGCGAC CGGATCGCGC TCTCCGAGCC CGGCTACAGC GACGACATGT GGCGGCGCTT CGCCGAGCTG GGCGCGATCG GCGCGCTCTT CCCCGAGGAC TGCGGCGGCC TCGGCGGCGC GGGCTTCGAC ATCGCGCTCG TGTTCGAGTG CCTCGGCCGC GGGCTCGTCG TCGAGCCGTT CGCGGGCGCG CTGATGACCG GGCGCGCGCT CGCGCAGGCG GGCGGCGACG CGCACCGCGC GCGGCTCGCC GATCTGATCG AAGGCCGTGC GATCGGTGCG TTCGCGCATG CGGAGCCCGG CACGCATTAC GAACCGTACA CGGTGAGAAC GCGCGCGGCT CGCGAAGGCG GGCGCTGGGT GCTTGACGGA GCGAAGGCCG TCGTCGATTG CGGCGAGCAC GCGACGTTCT TCGTCGTCAG CGCGCGCACC GCCGGCGCCG ACGACGCCGC CGCGGGCCTG TCGCTCTTCG TCGTGCCGAA GACGGCGCCG GGCGTGACGG CGCGCGGCTA CCGGAAGATC GACGGCGGCC GCGCGGCCGA CGTGCGCTTC GAGCGCGTCG CGCTGGCCGA CGACGCGCTC GTCGGCGCGC CGGGCGAAGC GGCCGACGCG ATCGAGCGCG CGCTCGGCTT CGGCCTGCTC GCGCTCGCGG CGGAGGCGCT TGGCGCGATG GACGTCGCGA AGGCGTACAC GCTCGAGTAT CTGCGCACCC GCCGCCAGTT CGGCGTGCCG ATCGGCAGCT TCCAGGCGCT GCAGCACCGG ATGGCGGAGC TGCTGCTCGA GATCGAGCAG GCGCGCTCGG CGGTGATCAA CGCGGCCGCG CAGCTCGACG CGCCGCGCGT GGCGCGCGAG CGCGCGCTGT CCGCCGCCAA GTACAGCGTC GGCCGGATCG GCGCGCTCGT CGCCGAGGAG AGCATCCAGC TGCACGGCGG GATCGGGATG ACGTGGGAGC TGCCGCTCGC GCACTACGCG AAACGGCTCG TGATGATCGA TCACCAGCTC GGCGACGAGG ATCACCACCT CGCGCGCTAT GTCGCGCTGT CGAAGCAA
Details for ButhA.00027.b.B1.GE36034
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMD FRYTEDRRML ADSLNRFIAE QYPFAARDRI ALSEPGYSDD MWRRFAELGA IGALFPEDCG GLGGAGFDIA LVFECLGRGL VVEPFAGALM TGRALAQAGG DAHRARLADL IEGRAIGAFA HAEPGTHYEP YTVRTRAARE GGRWVLDGAK AVVDCGEHAT FFVVSARTAG ADDAAAGLSL FVVPXTAPGV TARGYRKIDG GRAADVRFER VXLADXALVG APGEXAXAIE RALGFXXLAL AAEXLGAMDV XXAYTLICAP XASSACXXXX XXXXXPDXXL XXXNXXXRXX XXXXAXXXXX XXXXXXXXXX PXXXXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggac tttcgataca ccgaagaccg ccggatgctg gcggacagcc tgaaccgctt catcgccgag cagtacccgt tcgccgcgcg cgaccggatc gcgctctccg agcccggcta cagcgacgac atgtggcggc gcttcgccga gctgggcgcg atcggcgcgc tcttccccga ggactgcggc ggcctcggcg gcgcgggctt cgacatcgcg ctcgtgttcg agtgcctcgg ccgcgggctc gtcgtcgagc cgttcgcggg cgcgctgatg accgggcgcg cgctcgcgca ggcgggcggc gacgcgcacc gcgcgcggct cgccgatctg atcgaaggcc gtgcgatcgg tgcgttcgcg catgcggagc ccggcacgca ttacgaaccg tacacggtga gaacgcgcgc ggctcgcgaa ggcgggcgct gggtgcttga cggagcgaag gccgtcgtcg attgcggcga gcacgcgacg ttcttcgtcg tcagcgcgcg caccgccggc gccgacgacg ccgccgcggg cctgtcgctc ttcgtcgtgc cgaanacngc gccnggcgtg acggcgcgcg gctaccggaa gatcgacggc ggccgcgcgg ccgacgtgcg cttcgagcgc gtcncgctgg cngacnacgc gctcgtcggc gcgccnggcg aancggccga ngcgatcgag cgcgcgctcg gcttcngcnt gctcgcgctc gcggcngagg ngcttggcgc gatggacgtn ncgaangcnt acacgcttat ctgcgcaccc nccgccagtt cggcgtgcnn nnngcngcnt nnagnngntn cagnaccnga tgnngnnctn nnnnnnnnna atnnnnaggn ncggnnggng nnnnancngg ncgcnnnnnn cnanngccnc nngnnnnncn gnnncnnnnn ntgncnncnn cccannnnnn nnntcgnnnn nnnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMD FRYTEDRRML ADSLNRFIAE QYPFAARDRI ALSEPGYSDD MWRRFAELGA IGALFPEDCG GLGGAGFDIA LVFECLGRGL VVEPFAGALM TGRALAQAGG DAHRARLADL IEGRAIGAFA HAEPGTHYEP YTVRTRAARE GGRWVLDGAK AVVDCGEHAT FFVVSARTAG ADDAAAGLSL FVVPKTAPGV TARGYRKIDG GRAADVRFER VALADDALVG APGEAADAIE RALGFGLLAL AAEALGAMDV AKAYTLEYLR TRRQFGVPIG SFQALQHRMA ELLLEIEQAR SAVINAAAQL DAPRVARERA LSAAKYSVGR IGALVAEESI QLHGGIGMTW ELPLAHYAKR LVMIDHQLGD EDHHLARYVA LSKQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggact ttcgatacac cgaagaccgc cggatgctgg cggacagcct gaaccgcttc atcgccgagc agtacccgtt cgccgcgcgc gaccggatcg cgctctccga gcccggctac agcgacgaca tgtggcggcg cttcgccgag ctgggcgcga tcggcgcgct cttccccgag gactgcggcg gcctcggcgg cgcgggcttc gacatcgcgc tcgtgttcga gtgcctcggc cgcgggctcg tcgtcgagcc gttcgcgggc gcgctgatga ccgggcgcgc gctcgcgcag gcgggcggcg acgcgcaccg cgcgcggctc gccgatctga tcgaaggccg tgcgatcggt gcgttcgcgc atgcggagcc cggcacgcat tacgaaccgt acacggtgag aacgcgcgcg gctcgcgaag gcgggcgctg ggtgcttgac ggagcgaagg ccgtcgtcga ttgcggcgag cacgcgacgt tcttcgtcgt cagcgcgcgc accgccggcg ccgacgacgc cgccgcgggc ctgtcgctct tcgtcgtgcc gaagacggcg ccgggcgtga cggcgcgcgg ctaccggaag atcgacggcg gccgcgcggc cgacgtgcgc ttcgagcgcg tcgcgctggc cgacgacgcg ctcgtcggcg cgccgggcga agcggccgac gcgatcgagc gcgcgctcgg cttcggcctg ctcgcgctcg cggcggaggc gcttggcgcg atggacgtcg cgaaggcgta cacgctcgag tatctgcgca cccgccgcca gttcggcgtg ccgatcggca gcttccaggc gctgcagcac cggatggcgg agctgctgct cgagatcgag caggcgcgct cggcggtgat caacgcggcc gcgcagctcg acgcgccgcg cgtggcgcgc gagcgcgcgc tgtccgccgc caagtacagc gtcggccgga tcggcgcgct cgtcgccgag gagagcatcc agctgcacgg cgggatcggg atgacgtggg agctgccgct cgcgcactac gcgaaacggc tcgtgatgat cgatcaccag ctcggcgacg aggatcacca cctcgcgcgc tatgtcgcgc tgtcgaagca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat