SSGCID: Target Page

ButhA.00020.n

Methylmalonate-semialdehyde dehydrogenase

CENTER ID:	ButhA.00020.n
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.n.B1.GE36029	Full length( ButhA.00020.n )		1	509

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.n.B1.PS01720	Full length( ButhA.00020.n )		1	509

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2007
RefSeq:	YP_439995.1
UniProt:	Q2T4A4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNATPSSQKG HHVPTVKLLI AGEFVESHAT EWRDIVNPAT QELLARVPFA TAAEVDAAVE AAQAAFAKWK STPLSARMRI MLKFQDLVRA NLPQIAKTLT AEQGKTLPDA EGDVFRGLEV VEHACSIGTL QLGEFAENVA GGVDTYTLRQ PLGVCVGITP FNFPAMIPLW MFPMAIVCGN TFVLKPSEQD PLSTMLLVEL AIEAGVPKGV LNVVHGGKEV VDALCSHPLV KAISFVGSTA VGTHVYRLGS EHGKRVQSMM GAKNHAVILP DANREQTVNA LVGAAFGAAG QRCMATSVAV LVGAARDWLP DIVAKAKTLK VNAGAEAGTD VGPVVSRAAK QRILGLIETG AKEGAKLVLD GRDVSVPGYE HGNFIGPTIF ADVKPEMSVY THEIFGPVLC VMSVDTLDEA IALVNANPFG NGVGLFTQSG AAARKFQSEI DIGQVGINIP IPVPVPFFSF TGSRGSKLGD LGPYGKQVVQ FYTQTKTVTA RWFDDDATAG AVNTTIRLH

NT Sequence

ATGAACGCAA CTCCGTCGTC CCAGAAGGGA CATCACGTGC CGACCGTGAA GCTGTTGATC GCCGGCGAAT TCGTCGAATC CCATGCGACC GAGTGGCGTG ACATCGTCAA TCCGGCGACG CAGGAACTGC TCGCGCGCGT GCCGTTCGCG ACCGCGGCCG AGGTCGACGC GGCCGTCGAA GCCGCGCAGG CCGCGTTCGC GAAATGGAAG AGCACGCCGC TCTCCGCGCG CATGCGCATC ATGCTGAAGT TCCAGGATCT CGTGCGCGCG AACCTGCCGC AGATCGCGAA GACGCTGACG GCCGAGCAGG GCAAGACGCT GCCCGACGCC GAGGGCGACG TGTTCCGCGG CCTCGAGGTG GTCGAGCACG CGTGCTCGAT CGGCACGCTG CAGCTGGGCG AGTTCGCGGA GAACGTCGCG GGCGGCGTCG ATACGTACAC GCTGCGCCAG CCGCTTGGCG TGTGCGTCGG CATCACGCCG TTCAACTTCC CCGCGATGAT TCCGCTGTGG ATGTTCCCGA TGGCGATCGT CTGCGGCAAC ACGTTCGTGC TGAAGCCGTC CGAGCAGGAC CCGCTGTCGA CGATGCTGCT CGTCGAGCTC GCGATCGAGG CGGGCGTGCC GAAGGGCGTG CTCAACGTCG TGCACGGCGG CAAGGAAGTC GTCGACGCGC TGTGCTCGCA TCCGCTCGTG AAGGCGATTT CGTTCGTCGG CTCGACGGCC GTCGGCACGC ACGTGTACCG GCTCGGCAGC GAGCACGGCA AGCGCGTGCA ATCGATGATG GGTGCGAAGA ACCACGCGGT GATCCTGCCC GACGCGAACC GCGAGCAGAC GGTGAACGCG CTCGTCGGCG CGGCGTTCGG CGCGGCGGGC CAGCGCTGCA TGGCGACGTC GGTCGCGGTG CTCGTCGGCG CGGCGCGCGA CTGGCTGCCC GACATTGTCG CGAAGGCGAA GACGCTGAAG GTCAACGCGG GCGCGGAAGC GGGCACCGAC GTGGGCCCCG TCGTGTCGCG CGCGGCGAAG CAGCGAATTC TCGGCCTCAT CGAAACCGGC GCGAAGGAGG GCGCGAAGCT CGTGCTCGAC GGCCGCGACG TGAGCGTGCC CGGCTACGAG CACGGCAACT TCATCGGCCC GACGATCTTT GCGGACGTGA AGCCGGAGAT GTCGGTCTAT ACGCACGAGA TCTTCGGCCC GGTGCTGTGC GTGATGTCGG TCGACACGCT CGACGAAGCG ATCGCGCTCG TCAACGCGAA TCCGTTCGGC AACGGCGTCG GCCTCTTCAC GCAAAGCGGC GCGGCCGCGC GCAAGTTCCA GAGCGAGATC GACATCGGCC AGGTCGGCAT CAACATCCCG ATTCCGGTGC CGGTGCCGTT CTTCAGCTTC ACGGGCTCGC GCGGCTCGAA GCTCGGCGAT CTCGGCCCGT ACGGCAAGCA GGTCGTGCAG TTCTACACGC AGACGAAGAC CGTCACCGCG CGCTGGTTCG ACGACGACGC GACGGCGGGC GCCGTCAACA CGACGATTCG TCTGCAC

Details for ButhA.00020.n.B1.GE36029

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	54kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	76
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMN ATPSSQKGHH VPTVKLLIAG EFVESHATEW RDIVNPATQE LLARVPFATA AEVDAAVEAA QAAFAKWKST PLSARMRIML KFQDLVRANL PQIAKTLTAE QGKTLPDAEG DVFRGLEVVE HACSIGTLQL GEFAENVAGG VDTYTLRQPL GVCVGITPFN FPAMIPLWMF PMAIVCGNTF VLKPSEQDPL STMLLVELAI EAGVPKGVLN VVHGGKEVVD ALCSHPLVKA ISFVGSTAVX THVYRLGSEH GKRVQSMMGA KXHAVILPDA NREQXXTRSX XXVRRGGXAL HGDVXXXARX XXXXLXARXX XXXXXXXXXX XXXAGXXXAP XXXXXXXXXS SXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X

Validated NT Sequence

ttttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg caactccgtc gtcccagaag ggacatcacg tgccgaccgt gaagctgttg atcgccggcg aattcgtcga atcccatgcg accgagtggc gtgacatcgt caatccggcg acgcaggaac tgctcgcgcg cgtgccgttc gcgaccgcgg ccgaggtcga cgcggccgtc gaagccgcgc aggccgcgtt cgcgaaatgg aagagcacgc cgctctccgc gcgcatgcgc atcatgctga agttccagga tctcgtgcgc gcgaacctgc cgcagatcgc gaagacgctg acggccgagc agggcaagac gctgcccgac gccgagggcg acgtgttccg cggcctcgag gtggtcgagc acgcgtgctc gatcggcacg ctgcagctgg gcgagttcgc ggagaacgtc gcgggcggcg tcgatacgta cacgctgcgc cagccgcttg gcgtgtgcgt cggcatcacg ccgttcaact tccccgcgat gattccgctg tggatgttcc cgatggcgat cgtctgcggc aacacgttcg tgctgaagcc gtccgagcag gacccgctgt cgacgatgct gctcgtcgag ctcgcgatcg aggcgggcgt gccgaagggc gtgctcaacg tcgtgcacgg cggcaaggaa gtcgtcgacg cgctgtgctc gcatccgctc gtgaaggcga tttcgttcgt cggctcgacg gccgtcngca cgcacgtgta ccggctcggc agcgagcacg gcaagcgcgt gcaatcgatg atgggtgcga agaancacgc ggtgatcctg cccgacgcga accgcgagca gannngaacg cgctcgtnnn ncngngttcg gcgcggcggg ncagcgctgc atggcgacgt ngntnnnngn gctcgtnnnn nnnncnnnnn actggnngcc cganattnnn nnnnagnnna nnnncnnnnn tnannnnggn nnncngnagc nggnncnnnn nnggccccnn nnnnnnnnnn nnnnnnnnnc nncnnntcat cnnannnnnn nnannnnnnn nnnnnnnnnn nnnannnann nnnnnngnnn nnnnnnngnn nnnnnnnnnn nnnannnnnt nnnnnnnnnn nng

Expected Protein Sequence

MAHHHHHHMN ATPSSQKGHH VPTVKLLIAG EFVESHATEW RDIVNPATQE LLARVPFATA AEVDAAVEAA QAAFAKWKST PLSARMRIML KFQDLVRANL PQIAKTLTAE QGKTLPDAEG DVFRGLEVVE HACSIGTLQL GEFAENVAGG VDTYTLRQPL GVCVGITPFN FPAMIPLWMF PMAIVCGNTF VLKPSEQDPL STMLLVELAI EAGVPKGVLN VVHGGKEVVD ALCSHPLVKA ISFVGSTAVG THVYRLGSEH GKRVQSMMGA KNHAVILPDA NREQTVNALV GAAFGAAGQR CMATSVAVLV GAARDWLPDI VAKAKTLKVN AGAEAGTDVG PVVSRAAKQR ILGLIETGAK EGAKLVLDGR DVSVPGYEHG NFIGPTIFAD VKPEMSVYTH EIFGPVLCVM SVDTLDEAIA LVNANPFGNG VGLFTQSGAA ARKFQSEIDI GQVGINIPIP VPVPFFSFTG SRGSKLGDLG PYGKQVVQFY TQTKTVTARW FDDDATAGAV NTTIRLH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg caactccgtc gtcccagaag ggacatcacg tgccgaccgt gaagctgttg atcgccggcg aattcgtcga atcccatgcg accgagtggc gtgacatcgt caatccggcg acgcaggaac tgctcgcgcg cgtgccgttc gcgaccgcgg ccgaggtcga cgcggccgtc gaagccgcgc aggccgcgtt cgcgaaatgg aagagcacgc cgctctccgc gcgcatgcgc atcatgctga agttccagga tctcgtgcgc gcgaacctgc cgcagatcgc gaagacgctg acggccgagc agggcaagac gctgcccgac gccgagggcg acgtgttccg cggcctcgag gtggtcgagc acgcgtgctc gatcggcacg ctgcagctgg gcgagttcgc ggagaacgtc gcgggcggcg tcgatacgta cacgctgcgc cagccgcttg gcgtgtgcgt cggcatcacg ccgttcaact tccccgcgat gattccgctg tggatgttcc cgatggcgat cgtctgcggc aacacgttcg tgctgaagcc gtccgagcag gacccgctgt cgacgatgct gctcgtcgag ctcgcgatcg aggcgggcgt gccgaagggc gtgctcaacg tcgtgcacgg cggcaaggaa gtcgtcgacg cgctgtgctc gcatccgctc gtgaaggcga tttcgttcgt cggctcgacg gccgtcggca cgcacgtgta ccggctcggc agcgagcacg gcaagcgcgt gcaatcgatg atgggtgcga agaaccacgc ggtgatcctg cccgacgcga accgcgagca gacggtgaac gcgctcgtcg gcgcggcgtt cggcgcggcg ggccagcgct gcatggcgac gtcggtcgcg gtgctcgtcg gcgcggcgcg cgactggctg cccgacattg tcgcgaaggc gaagacgctg aaggtcaacg cgggcgcgga agcgggcacc gacgtgggcc ccgtcgtgtc gcgcgcggcg aagcagcgaa ttctcggcct catcgaaacc ggcgcgaagg agggcgcgaa gctcgtgctc gacggccgcg acgtgagcgt gcccggctac gagcacggca acttcatcgg cccgacgatc tttgcggacg tgaagccgga gatgtcggtc tatacgcacg agatcttcgg cccggtgctg tgcgtgatgt cggtcgacac gctcgacgaa gcgatcgcgc tcgtcaacgc gaatccgttc ggcaacggcg tcggcctctt cacgcaaagc ggcgcggccg cgcgcaagtt ccagagcgag atcgacatcg gccaggtcgg catcaacatc ccgattccgg tgccggtgcc gttcttcagc ttcacgggct cgcgcggctc gaagctcggc gatctcggcc cgtacggcaa gcaggtcgtg cagttctaca cgcagacgaa gaccgtcacc gcgcgctggt tcgacgacga cgcgacggcg ggcgccgtca acacgacgat tcgtctgcac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for ButhA.00020.n.B1.PS01720

PURIFICATION DATe:	1/8/2013
CONCENTRATION:	101mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	76
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMN ATPSSQKGHH VPTVKLLIAG EFVESHATEW RDIVNPATQE LLARVPFATA AEVDAAVEAA QAAFAKWKST PLSARMRIML KFQDLVRANL PQIAKTLTAE QGKTLPDAEG DVFRGLEVVE HACSIGTLQL GEFAENVAGG VDTYTLRQPL GVCVGITPFN FPAMIPLWMF PMAIVCGNTF VLKPSEQDPL STMLLVELAI EAGVPKGVLN VVHGGKEVVD ALCSHPLVKA ISFVGSTAVG THVYRLGSEH GKRVQSMMGA KNHAVILPDA NREQTVNALV GAAFGAAGQR CMATSVAVLV GAARDWLPDI VAKAKTLKVN AGAEAGTDVG PVVSRAAKQR ILGLIETGAK EGAKLVLDGR DVSVPGYEHG NFIGPTIFAD VKPEMSVYTH EIFGPVLCVM SVDTLDEAIA LVNANPFGNG VGLFTQSGAA ARKFQSEIDI GQVGINIPIP VPVPFFSFTG SRGSKLGDLG PYGKQVVQFY TQTKTVTARW FDDDATAGAV NTTIRLH

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG CAACTCCGTC GTCCCAGAAG GGACATCACG TGCCGACCGT GAAGCTGTTG ATCGCCGGCG AATTCGTCGA ATCCCATGCG ACCGAGTGGC GTGACATCGT CAATCCGGCG ACGCAGGAAC TGCTCGCGCG CGTGCCGTTC GCGACCGCGG CCGAGGTCGA CGCGGCCGTC GAAGCCGCGC AGGCCGCGTT CGCGAAATGG AAGAGCACGC CGCTCTCCGC GCGCATGCGC ATCATGCTGA AGTTCCAGGA TCTCGTGCGC GCGAACCTGC CGCAGATCGC GAAGACGCTG ACGGCCGAGC AGGGCAAGAC GCTGCCCGAC GCCGAGGGCG ACGTGTTCCG CGGCCTCGAG GTGGTCGAGC ACGCGTGCTC GATCGGCACG CTGCAGCTGG GCGAGTTCGC GGAGAACGTC GCGGGCGGCG TCGATACGTA CACGCTGCGC CAGCCGCTTG GCGTGTGCGT CGGCATCACG CCGTTCAACT TCCCCGCGAT GATTCCGCTG TGGATGTTCC CGATGGCGAT CGTCTGCGGC AACACGTTCG TGCTGAAGCC GTCCGAGCAG GACCCGCTGT CGACGATGCT GCTCGTCGAG CTCGCGATCG AGGCGGGCGT GCCGAAGGGC GTGCTCAACG TCGTGCACGG CGGCAAGGAA GTCGTCGACG CGCTGTGCTC GCATCCGCTC GTGAAGGCGA TTTCGTTCGT CGGCTCGACG GCCGTCGGCA CGCACGTGTA CCGGCTCGGC AGCGAGCACG GCAAGCGCGT GCAATCGATG ATGGGTGCGA AGAACCACGC GGTGATCCTG CCCGACGCGA ACCGCGAGCA GACGGTGAAC GCGCTCGTCG GCGCGGCGTT CGGCGCGGCG GGCCAGCGCT GCATGGCGAC GTCGGTCGCG GTGCTCGTCG GCGCGGCGCG CGACTGGCTG CCCGACATTG TCGCGAAGGC GAAGACGCTG AAGGTCAACG CGGGCGCGGA AGCGGGCACC GACGTGGGCC CCGTCGTGTC GCGCGCGGCG AAGCAGCGAA TTCTCGGCCT CATCGAAACC GGCGCGAAGG AGGGCGCGAA GCTCGTGCTC GACGGCCGCG ACGTGAGCGT GCCCGGCTAC GAGCACGGCA ACTTCATCGG CCCGACGATC TTTGCGGACG TGAAGCCGGA GATGTCGGTC TATACGCACG AGATCTTCGG CCCGGTGCTG TGCGTGATGT CGGTCGACAC GCTCGACGAA GCGATCGCGC TCGTCAACGC GAATCCGTTC GGCAACGGCG TCGGCCTCTT CACGCAAAGC GGCGCGGCCG CGCGCAAGTT CCAGAGCGAG ATCGACATCG GCCAGGTCGG CATCAACATC CCGATTCCGG TGCCGGTGCC GTTCTTCAGC TTCACGGGCT CGCGCGGCTC GAAGCTCGGC GATCTCGGCC CGTACGGCAA GCAGGTCGTG CAGTTCTACA CGCAGACGAA GACCGTCACC GCGCGCTGGT TCGACGACGA CGCGACGGCG GGCGCCGTCA ACACGACGAT TCGTCTGCAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT