ButhA.00020.l

Glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent

CENTER ID: ButhA.00020.l
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00020.l.B1.GE36027 Full length( ButhA.00020.l ) 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2260
RefSeq: YP_440237.1
UniProt: Q2T3L2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPPPLRDHP AYRRETLRWC GQRAARARTL DVFDPYSGRR VGTVPLATVD DVRRAFDYAG AYRPTLSRYE RSQILERAAA RLVACAEEAS TLISLESGLS KQDSRYEIGR VADVFKFASI EALRDDAQSY SCDLTPHGTS RRVFSQRQPL DGVIVAITPF NHPMNQVAHK IAPAIATNNR VIVKPSEKVP LSALYLADVL YEAGLPEPML QVLTGDPREI ADELLTHPSA ALITFTGGVA IGKSIAAKAG YRRIVLELGG NDPLIVLDDA DLERAATLAA QGSYKNSGQR CTAVKRILVQ KRVAPRFTEL LVEHTRAWTY GDPFDPTNRM GTVIDEAAAA LFEARVDEAV ARGARLLTGN ARRGALYAPT VLDRVDASMT LVREETFGPV SPIIAFDTID DAIRISNGTA FGLSSGVCTD RADAIVRFVN ELNVGTVNVW EVPGYRLELT PFGGIKDSGL GYKEGVQEAM KSFTNLKTFS LPWA
NT Sequence
ATGACGCCGC CGCCGCTTCG CGATCACCCC GCTTACCGCC GCGAGACGCT GCGCTGGTGC GGGCAACGGG CGGCGCGCGC GCGCACGCTC GACGTATTCG ACCCGTATTC GGGGCGGCGC GTCGGCACGG TGCCGCTTGC GACGGTCGAC GACGTGCGCC GCGCGTTCGA CTATGCGGGC GCGTACCGGC CGACGCTGTC GCGCTACGAG CGCTCGCAGA TACTCGAGCG CGCGGCCGCG CGCCTCGTTG CCTGCGCGGA AGAGGCGTCC ACGCTGATCT CGCTCGAATC GGGCCTGTCG AAACAGGATT CGCGTTACGA GATCGGCCGC GTCGCGGACG TGTTCAAGTT CGCGTCGATC GAGGCGTTGC GCGACGACGC GCAGAGCTAT TCGTGCGATC TGACGCCGCA CGGCACGTCG CGCCGCGTGT TCTCGCAGCG GCAGCCGCTC GACGGCGTGA TCGTCGCGAT CACGCCGTTC AATCATCCGA TGAACCAGGT CGCGCACAAG ATCGCGCCGG CGATCGCGAC GAACAATCGC GTGATCGTGA AGCCGTCGGA GAAGGTGCCG CTGTCGGCGC TGTATCTCGC CGACGTGCTG TACGAAGCGG GCCTGCCCGA GCCGATGCTG CAGGTGCTGA CGGGCGATCC GCGCGAAATC GCGGACGAAC TGCTGACGCA CCCGAGCGCG GCGCTGATCA CGTTCACGGG CGGCGTCGCG ATCGGCAAGT CCATCGCGGC GAAGGCCGGC TACCGGCGCA TCGTGCTCGA GCTGGGCGGC AACGATCCGC TGATCGTGCT CGACGACGCC GACCTCGAAC GCGCGGCGAC GCTCGCCGCG CAGGGCTCGT ACAAGAATTC CGGCCAGCGC TGCACGGCGG TCAAGCGCAT CCTCGTGCAG AAGCGCGTCG CGCCACGCTT CACGGAGTTG CTCGTCGAGC ACACGCGCGC GTGGACGTAC GGCGATCCGT TCGACCCGAC GAACCGGATG GGGACGGTGA TCGACGAGGC GGCGGCCGCG CTGTTCGAGG CGCGCGTCGA CGAAGCGGTG GCGCGGGGCG CGCGCCTGCT GACAGGCAAC GCGCGGCGCG GCGCGCTGTA TGCGCCGACG GTGCTTGATC GCGTCGACGC ATCGATGACG CTCGTGCGCG AGGAGACGTT CGGGCCCGTG TCGCCGATCA TCGCGTTCGA CACGATCGAC GACGCGATCC GGATCAGCAA CGGCACCGCG TTCGGCCTGT CGTCGGGCGT GTGCACGGAC CGCGCGGATG CGATCGTGCG CTTCGTCAAC GAGCTGAACG TCGGCACGGT AAACGTGTGG GAGGTGCCGG GCTACCGGCT TGAGCTGACG CCGTTCGGCG GCATCAAGGA TTCCGGGCTC GGATACAAGG AAGGGGTGCA GGAGGCGATG AAGAGCTTCA CGAACCTGAA GACATTCTCC CTGCCGTGGG CG
Details for ButhA.00020.l.B1.GE36027
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 73
Validated AA Sequence
MAHHHHHHMT PPPLRDHPAY RRETLRWCGQ RAARARTLDV FDPYSGRRVG TVPLATVDDV RRAFDYAGAY RPTLSRYERS QILERAAARL VACAEEASTL ISLESGLSKQ DSRYEIGRVA DVFKFASIEA LRDDAQSYSC DLTPHGTSRR VFSQRQPLDG VIVAITPFNH PMNQVAHKIA PAIATNNRVI VKPSEKVPLS ALYLADVLYE AGLPEPMLQV LTGDPREIAD ELLTHPSAAL ITFTGGVAIG KSIAAXXGYR RIVLELGGND PLIVLDDAXL ERAATLAAXX RTRXXXSAAR RQAHXXXEAR RXXLXXXLXE XXXXXXXXXX RXXXXGXXRX AXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttta ctttaagaag gagntntacc atggctcacc accaccacca ccatatgacg ccgccgccgc ttcgcgatca ccccgcttac cgccgcgaga cgctgcgctg gtgcgggcaa cgggcggcgc gcgcgcgcac gctcgacgta ttcgacccgt attcggggcg gcgcgtcggc acggtgccgc ttgcgacggt cgacgacgtg cgccgcgcgt tcgactatgc gggcgcgtac cggccgacgc tgtcgcgcta cgagcgctcg cagatactcg agcgcgcggc cgcgcgcctc gttgcctgcg cggaagaggc gtccacgctg atctcgctcg aatcgggcct gtcgaaacag gattcgcgtt acgagatcgg ccgcgtcgcg gacgtgttca agttcgcgtc gatcgaggcg ttgcgcgacg acgcgcagag ctattcgtgc gatctgacgc cgcacggcac gtcgcgccgc gtgttctcgc agcggcagcc gctcgacggc gtgatcgtcg cgatcacgcc gttcaatcat ccgatgaacc aggtcgcgca caagatcgcg ccggcgatcg cgacgaacaa tcgcgtgatc gtgaagccgt cggagaaggt gccgctgtcg gcgctgtatc tcgccgacgt gctgtacgaa gcgggcctgc ccgagccgat gctgcaggtg ctgacgggcg atccgcgcga aatcgcggac gaactgctga cgcacccgag cgcggcgctg atcacgttca cgggcggcgt cgcgatcggc aagtccatcg cggcgaannn cggctaccgg cgcatcgtgc tcgagctggg cggcaacgat ccgctgatcg tgctcgacga cgccganctc gaacgcgcgg cgacnctcgc cgcgcnngnt cgtacaagan ttncnnccag cgctgcacgg cgtcaagcgc atcntcntgn ngaagcgcgn cgcgncncgc ttcangnant tctcgncgag cnnncgcncn ngnnnnngtn nnnnancntn cgannnnncn nnnggggnnn nnatcgacna gcgnnnnnnn cnnnnnangn nnnnnnnnan nnnnnnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn t
Expected Protein Sequence
MAHHHHHHMT PPPLRDHPAY RRETLRWCGQ RAARARTLDV FDPYSGRRVG TVPLATVDDV RRAFDYAGAY RPTLSRYERS QILERAAARL VACAEEASTL ISLESGLSKQ DSRYEIGRVA DVFKFASIEA LRDDAQSYSC DLTPHGTSRR VFSQRQPLDG VIVAITPFNH PMNQVAHKIA PAIATNNRVI VKPSEKVPLS ALYLADVLYE AGLPEPMLQV LTGDPREIAD ELLTHPSAAL ITFTGGVAIG KSIAAKAGYR RIVLELGGND PLIVLDDADL ERAATLAAQG SYKNSGQRCT AVKRILVQKR VAPRFTELLV EHTRAWTYGD PFDPTNRMGT VIDEAAAALF EARVDEAVAR GARLLTGNAR RGALYAPTVL DRVDASMTLV REETFGPVSP IIAFDTIDDA IRISNGTAFG LSSGVCTDRA DAIVRFVNEL NVGTVNVWEV PGYRLELTPF GGIKDSGLGY KEGVQEAMKS FTNLKTFSLP WA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc cgccgccgct tcgcgatcac cccgcttacc gccgcgagac gctgcgctgg tgcgggcaac gggcggcgcg cgcgcgcacg ctcgacgtat tcgacccgta ttcggggcgg cgcgtcggca cggtgccgct tgcgacggtc gacgacgtgc gccgcgcgtt cgactatgcg ggcgcgtacc ggccgacgct gtcgcgctac gagcgctcgc agatactcga gcgcgcggcc gcgcgcctcg ttgcctgcgc ggaagaggcg tccacgctga tctcgctcga atcgggcctg tcgaaacagg attcgcgtta cgagatcggc cgcgtcgcgg acgtgttcaa gttcgcgtcg atcgaggcgt tgcgcgacga cgcgcagagc tattcgtgcg atctgacgcc gcacggcacg tcgcgccgcg tgttctcgca gcggcagccg ctcgacggcg tgatcgtcgc gatcacgccg ttcaatcatc cgatgaacca ggtcgcgcac aagatcgcgc cggcgatcgc gacgaacaat cgcgtgatcg tgaagccgtc ggagaaggtg ccgctgtcgg cgctgtatct cgccgacgtg ctgtacgaag cgggcctgcc cgagccgatg ctgcaggtgc tgacgggcga tccgcgcgaa atcgcggacg aactgctgac gcacccgagc gcggcgctga tcacgttcac gggcggcgtc gcgatcggca agtccatcgc ggcgaaggcc ggctaccggc gcatcgtgct cgagctgggc ggcaacgatc cgctgatcgt gctcgacgac gccgacctcg aacgcgcggc gacgctcgcc gcgcagggct cgtacaagaa ttccggccag cgctgcacgg cggtcaagcg catcctcgtg cagaagcgcg tcgcgccacg cttcacggag ttgctcgtcg agcacacgcg cgcgtggacg tacggcgatc cgttcgaccc gacgaaccgg atggggacgg tgatcgacga ggcggcggcc gcgctgttcg aggcgcgcgt cgacgaagcg gtggcgcggg gcgcgcgcct gctgacaggc aacgcgcggc gcggcgcgct gtatgcgccg acggtgcttg atcgcgtcga cgcatcgatg acgctcgtgc gcgaggagac gttcgggccc gtgtcgccga tcatcgcgtt cgacacgatc gacgacgcga tccggatcag caacggcacc gcgttcggcc tgtcgtcggg cgtgtgcacg gaccgcgcgg atgcgatcgt gcgcttcgtc aacgagctga acgtcggcac ggtaaacgtg tgggaggtgc cgggctaccg gcttgagctg acgccgttcg gcggcatcaa ggattccggg ctcggataca aggaaggggt gcaggaggcg atgaagagct tcacgaacct gaagacattc tccctgccgt gggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat