ButhA.00020.k

Aldehyde dehydrogenase family protein

CENTER ID: ButhA.00020.k
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00020.k.B1.GE36026 Full length( ButhA.00020.k ) 1 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2305
RefSeq: YP_440280.1
UniProt: Q2T3G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLINGLCIEG SGEPLSIVDP ATGEPLASPA AASAADVERA VAAAEAAFPA WRATTPATRA SLLLALADEI ERHAHALAQI ESRNTGKPLH LVVQDEMPAV ADCFRFYAGA ARTASGPSAG EYVEGHTSMV RRDPVGVVAQ IAPWNYPLMM AAWKLAPALA AGNTIVFKPS EWTPLSIVAL EAALARIFPA GVVNVVLGDG ANVGRALATH PRVRMISLTG SVEAGKSVLA AAARNLKRTH LELGGKAPVL VFDDADLDAA VAGIRYAGFY NAGQDCTAAT RIYAQRGVYD ALAQRLADAA STLRIGPPDR ADAEMGPLVS AAHRARVDRF VREAAALPHA SVLTGGAPLP GPGCYYAPTV IAGVRHDDAP MRREAFGPVV TLTPFDTESQ ALRWANDSEY GLASSVWTRD AARGMRLAAC IEAGVTWVNA HFTYTADMPH GGTKQSGYGS DLSTLGLADY TQPRHVMWRH
NT Sequence
ATGCTGATTA ACGGCCTTTG CATCGAAGGA AGCGGCGAGC CGCTGTCGAT CGTCGATCCG GCGACGGGCG AGCCGCTCGC ATCGCCCGCC GCCGCGAGCG CGGCCGATGT CGAACGCGCG GTCGCGGCCG CCGAAGCCGC GTTCCCCGCA TGGCGCGCGA CGACGCCGGC CACGCGCGCG AGCCTGCTGC TCGCGCTCGC CGACGAAATC GAGCGGCACG CGCACGCGCT CGCGCAAATC GAAAGCCGCA ACACCGGCAA GCCGCTGCAT CTCGTCGTGC AGGACGAGAT GCCCGCCGTC GCCGACTGTT TCCGCTTCTA CGCGGGTGCT GCGCGCACCG CGAGCGGGCC GTCCGCGGGC GAATACGTCG AGGGCCACAC GAGCATGGTC CGCCGCGATC CCGTCGGCGT GGTCGCGCAA ATCGCGCCGT GGAACTACCC GCTGATGATG GCCGCATGGA AACTCGCGCC GGCGCTCGCG GCCGGCAACA CGATCGTGTT CAAGCCGTCG GAATGGACGC CGCTGTCGAT CGTCGCGCTC GAAGCCGCGC TCGCGCGCAT CTTTCCGGCG GGCGTCGTCA ACGTCGTGCT CGGCGACGGC GCGAACGTCG GCCGCGCGCT CGCGACGCAT CCGCGCGTGC GGATGATTTC GCTGACGGGC TCGGTCGAGG CCGGCAAGTC CGTGCTCGCC GCGGCGGCCA GAAACCTGAA GCGCACGCAT CTGGAGCTCG GCGGCAAGGC GCCCGTGCTC GTGTTCGACG ACGCCGACCT CGACGCGGCC GTCGCCGGCA TCCGCTACGC GGGCTTCTAC AACGCCGGGC AGGACTGCAC GGCGGCCACG CGAATCTACG CGCAGCGCGG CGTCTATGAC GCGCTCGCGC AGCGGCTCGC CGATGCGGCG AGCACGCTGC GCATCGGCCC GCCCGATCGC GCGGACGCCG AGATGGGGCC GCTCGTCAGC GCCGCGCATC GCGCGCGCGT CGACCGTTTC GTGCGGGAAG CGGCCGCGCT GCCGCATGCG AGCGTGCTCA CGGGCGGCGC GCCGCTTCCC GGCCCCGGCT GCTACTACGC GCCGACCGTC ATCGCCGGCG TGCGCCACGA CGACGCGCCG ATGCGCCGCG AAGCGTTCGG CCCGGTCGTC ACGCTGACGC CGTTCGACAC CGAATCGCAA GCGCTCAGGT GGGCGAACGA TTCGGAATAC GGGCTGGCTT CGTCGGTATG GACGCGCGAC GCGGCGCGCG GCATGCGGCT CGCCGCGTGC ATCGAGGCGG GCGTCACGTG GGTGAACGCG CATTTCACCT ACACGGCCGA CATGCCGCAC GGCGGGACCA AGCAGTCCGG CTACGGCTCC GATCTGTCGA CGCTCGGCCT CGCCGACTAC ACGCAGCCGC GCCACGTGAT GTGGCGGCAT
Details for ButhA.00020.k.B1.GE36026
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHML INGLCIEGSG EPLSIVDPAT GEPLASPAAA SAADVERAVA AAEAAFPAWR ATTPATRASL LLALADEIER HAHALAQIES RNTGKPLHLV VQDEMPAVAD CFRFYAGAAR TASGPSAGEY VEGHTSMVRR DPVGVVAQIA PWNYPLMMAA WKLAPALAAG NTIVFKPSEW TPLSIVALEA ALARIFPAGV VNVVLGDGAN VGRALATHPR VRMISLTGSV EAGKSVLAAA ARNLKRTHLE LGGKAPVLVF DDADLDAAVA XIRYAGFYNA GXDCTAATRI YAQRGVYDAL AQRLADAAST LRIXPPXRXX XXXGPLVSXX HXAXXXXXXX XXXXXHXXXX XXGXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttnntnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatgctgat taacggcctt tgcatcgaag gaagcggcga gccgctgtcg atcgtcgatc cggcgacggg cgagccgctc gcatcgcccg ccgccgcgag cgcggccgat gtcgaacgcg cggtcgcggc cgccgaagcc gcgttccccg catggcgcgc gacgacgccg gccacgcgcg cgagcctgct gctcgcgctc gccgacgaaa tcgagcggca cgcgcacgcg ctcgcgcaaa tcgaaagccg caacaccggc aagccgctgc atctcgtcgt gcaggacgag atgcccgccg tcgccgactg tttccgcttc tacgcgggtg ctgcgcgcac cgcgagcggg ccgtccgcgg gcgaatacgt cgagggccac acgagcatgg tccgccgcga tcccgtcggc gtggtcgcgc aaatcgcgcc gtggaactac ccgctgatga tggccgcatg gaaactcgcg ccggcgctcg cggccggcaa cacgatcgtg ttcaagccgt cggaatggac gccgctgtcg atcgtcgcgc tcgaagccgc gctcgcgcgc atctttccgg cgggcgtcgt caacgtcgtg ctcggcgacg gcgcgaacgt cggccgcgcg ctcgcgacgc atccgcgcgt gcggatgatt tcgctgacgg gctcggtcga ggccggcaag tccgtgctcg ccgcggcggc cagaaacctg aagcgcacgc atctggagct cggcggcaag gcgcccgtgc tcgtgttcga cgacgccgac ctcgacgcgg ccgtcgcnng natccgctac gcgggcttct acaacgccgg gcangactgc acggcggcca cgcgaatcta cgcgcagcgc ggcgtctatg acgcgctcgc gcagcggctc gccgatgcng cgagcacgct gcgcatcnnc ccncccnatc gnncnnnnnc cgannngggg ccnctcgtca gcnnnnngca tcncgcgcnn cnnnnnnttc nngnngnnnn nnnnnnnnnn nnncatgnnn nnnnnntnnn ggnnggnnnn nccnnntncn nnnncnnnnn nnntannann nnnncncnnt nnnnnnnnng nnnnnnnnnn nnnannnnnn nnnnnnnncn cnnnnnnnnn nnnnnnnnnn nncnnnnnnn nnnnnc
Expected Protein Sequence
MAHHHHHHML INGLCIEGSG EPLSIVDPAT GEPLASPAAA SAADVERAVA AAEAAFPAWR ATTPATRASL LLALADEIER HAHALAQIES RNTGKPLHLV VQDEMPAVAD CFRFYAGAAR TASGPSAGEY VEGHTSMVRR DPVGVVAQIA PWNYPLMMAA WKLAPALAAG NTIVFKPSEW TPLSIVALEA ALARIFPAGV VNVVLGDGAN VGRALATHPR VRMISLTGSV EAGKSVLAAA ARNLKRTHLE LGGKAPVLVF DDADLDAAVA GIRYAGFYNA GQDCTAATRI YAQRGVYDAL AQRLADAAST LRIGPPDRAD AEMGPLVSAA HRARVDRFVR EAAALPHASV LTGGAPLPGP GCYYAPTVIA GVRHDDAPMR REAFGPVVTL TPFDTESQAL RWANDSEYGL ASSVWTRDAA RGMRLAACIE AGVTWVNAHF TYTADMPHGG TKQSGYGSDL STLGLADYTQ PRHVMWRH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga ttaacggcct ttgcatcgaa ggaagcggcg agccgctgtc gatcgtcgat ccggcgacgg gcgagccgct cgcatcgccc gccgccgcga gcgcggccga tgtcgaacgc gcggtcgcgg ccgccgaagc cgcgttcccc gcatggcgcg cgacgacgcc ggccacgcgc gcgagcctgc tgctcgcgct cgccgacgaa atcgagcggc acgcgcacgc gctcgcgcaa atcgaaagcc gcaacaccgg caagccgctg catctcgtcg tgcaggacga gatgcccgcc gtcgccgact gtttccgctt ctacgcgggt gctgcgcgca ccgcgagcgg gccgtccgcg ggcgaatacg tcgagggcca cacgagcatg gtccgccgcg atcccgtcgg cgtggtcgcg caaatcgcgc cgtggaacta cccgctgatg atggccgcat ggaaactcgc gccggcgctc gcggccggca acacgatcgt gttcaagccg tcggaatgga cgccgctgtc gatcgtcgcg ctcgaagccg cgctcgcgcg catctttccg gcgggcgtcg tcaacgtcgt gctcggcgac ggcgcgaacg tcggccgcgc gctcgcgacg catccgcgcg tgcggatgat ttcgctgacg ggctcggtcg aggccggcaa gtccgtgctc gccgcggcgg ccagaaacct gaagcgcacg catctggagc tcggcggcaa ggcgcccgtg ctcgtgttcg acgacgccga cctcgacgcg gccgtcgccg gcatccgcta cgcgggcttc tacaacgccg ggcaggactg cacggcggcc acgcgaatct acgcgcagcg cggcgtctat gacgcgctcg cgcagcggct cgccgatgcg gcgagcacgc tgcgcatcgg cccgcccgat cgcgcggacg ccgagatggg gccgctcgtc agcgccgcgc atcgcgcgcg cgtcgaccgt ttcgtgcggg aagcggccgc gctgccgcat gcgagcgtgc tcacgggcgg cgcgccgctt cccggccccg gctgctacta cgcgccgacc gtcatcgccg gcgtgcgcca cgacgacgcg ccgatgcgcc gcgaagcgtt cggcccggtc gtcacgctga cgccgttcga caccgaatcg caagcgctca ggtgggcgaa cgattcggaa tacgggctgg cttcgtcggt atggacgcgc gacgcggcgc gcggcatgcg gctcgccgcg tgcatcgagg cgggcgtcac gtgggtgaac gcgcatttca cctacacggc cgacatgccg cacggcggga ccaagcagtc cggctacggc tccgatctgt cgacgctcgg cctcgccgac tacacgcagc cgcgccacgt gatgtggcgg cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat