SSGCID: Target Page

ButhA.00020.i

Aldehyde dehydrogenase (NAD) family protein superfamily

CENTER ID:	ButhA.00020.i
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.i.B1.GE36025	Full length( ButhA.00020.i )		1	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.i.B1.PS01823	Full length( ButhA.00020.i )		1	485

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2388
RefSeq:	YP_440350.1
UniProt:	Q2T399

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSASHALHW IDGEWVDSPA RRDSFDPATG AVIGRYADGG ETEARAAVAA ASRAFARTRW KHDAALRAKA LDELALAFER HTEALIDQLS LENGKIKPEA RFEVEMAPSK LRYYAALARA ERAHGGTPRP DVVSLVLREP MGVAGIIVPW NSPVVLMVRA LAPALAAGAS AVVKMPGQTA QTNALVAKVL SEAPSLPRGS VNVFSESGAD GAKYLVASPD VPVISFTGST ATGRAISAAG AQRLKRFGLE LGGKTPHVVF DDADLDAALP VLAKSLTVFA GQFCMTGSRL LVQRNVAEAV RTRLAATLGA VRVGPAADPR SEMGPLIDRA NVARVDRSVD QAIAAGAKVL LRGGPATDGA LAAGAFYRPT LLEVDDPKLD IVQTETFGPV MTLQVFDTED EAVALANDSE YGLAAAVWTR DVQRSMRVAR ALQAGTVWIN DWAKVYDEFE EGGYRQSGLG RLNGAAAIDD FIEYKHITLS TGASQ

NT Sequence

ATGACTTCCG CCTCTCACGC TTTACACTGG ATCGACGGCG AATGGGTCGA TTCGCCCGCT CGTCGCGATT CGTTCGATCC GGCGACCGGC GCCGTGATCG GCCGCTATGC CGACGGCGGC GAAACCGAAG CGCGCGCGGC CGTCGCGGCG GCGTCCCGCG CGTTCGCACG CACGCGCTGG AAGCACGACG CCGCATTGCG CGCCAAGGCT CTCGACGAAC TCGCACTCGC ATTCGAACGG CACACCGAAG CGCTCATCGA CCAGCTCTCG CTCGAAAACG GCAAGATCAA GCCGGAAGCC CGGTTCGAGG TCGAGATGGC GCCCTCGAAG CTGCGCTATT ACGCGGCCCT CGCGCGCGCC GAGCGCGCCC ACGGCGGAAC GCCGCGGCCG GACGTCGTAT CGCTCGTGCT GCGGGAGCCG ATGGGCGTGG CCGGCATCAT CGTGCCGTGG AATTCCCCCG TCGTCCTGAT GGTGCGCGCG CTCGCGCCGG CGCTTGCGGC CGGCGCGAGC GCGGTCGTCA AGATGCCGGG CCAGACCGCG CAAACGAACG CCCTCGTCGC CAAGGTGCTG TCGGAAGCGC CGTCGCTGCC GCGCGGCAGC GTCAACGTGT TCAGCGAATC CGGCGCCGAC GGCGCGAAAT ACCTCGTCGC TTCTCCGGAC GTGCCCGTCA TCAGCTTCAC CGGCTCGACG GCGACCGGCC GCGCGATCTC GGCGGCCGGC GCACAGCGCC TGAAGCGCTT CGGCCTCGAG CTCGGCGGCA AGACACCCCA TGTCGTATTC GACGACGCCG ATCTCGACGC CGCGCTGCCG GTTCTCGCGA AGTCGCTGAC CGTATTCGCC GGCCAGTTCT GCATGACGGG CTCGCGGCTG CTCGTGCAGC GAAACGTGGC GGAAGCGGTG CGCACGCGTC TCGCGGCGAC ACTCGGCGCG GTGCGCGTCG GCCCCGCCGC CGATCCGCGC AGCGAAATGG GGCCCCTCAT CGACCGGGCG AACGTCGCGC GCGTCGATCG CAGCGTCGAT CAGGCGATCG CCGCGGGCGC GAAGGTCCTG CTGCGCGGCG GCCCGGCCAC CGACGGCGCA CTCGCGGCAG GCGCGTTCTA CCGCCCGACG CTCCTCGAAG TCGACGATCC GAAGCTCGAC ATCGTGCAGA CCGAAACGTT CGGCCCGGTA ATGACGCTGC AGGTGTTCGA CACGGAAGAC GAAGCAGTCG CGCTCGCCAA CGACAGCGAA TATGGCCTCG CCGCCGCGGT ATGGACGCGC GACGTGCAAC GCTCGATGCG GGTCGCGCGC GCACTGCAAG CCGGGACGGT ATGGATCAAC GATTGGGCGA AGGTCTACGA CGAATTCGAG GAAGGCGGCT ACCGCCAGTC CGGCCTCGGC CGCCTCAACG GCGCCGCCGC CATCGACGAT TTCATCGAGT ACAAGCACAT CACGCTGAGC ACTGGGGCAT CGCAA

Details for ButhA.00020.i.B1.GE36025

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	52kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	28
PERCENT COVERAGE:	93

Validated AA Sequence

MGRFARSSRF VRSGDRRRDR PLCRRRRNRS ARGRRGGVPR VRTHALEARR RIARQGSRRT RTRIRTAHRS AHRPALARKR QDQAGSPVRG RDGALEAALL RGPRARRARP RRNAAAGRRI ARAAGADGRG RHHRAVEFPR RPDGARARAG ACGRRERGRQ DAGPDRANER PRRQGAVGSA VAAARQRQRV QRIRRRRREI PRRFSGRARH QLHRLDXDRP RDLGGRRTAP EALRPRARRQ DTPCRIRRRR SRRRAAGSRE VADRIRRPVL HDGLAAARAA KRGGSGAHAS RGDTRRGARR PRRRSAQRNG APHRPGERRA RRSQRRSGDR RGREGPAARR PGHRRRTRGR RVLPPDAPRS RRSEARHRAD RNVRPGNDAA GVRHGRRSSR ARQRQRIWPR RRGMDARRAT LDAGRARTAS RDGMDQRLGE GLRRIRGRRL PPVRPRPPQR RRRHRRFHRV QAH

Validated NT Sequence

gatgtgcttg tactcgatga aatcgtcgat ggcggcggcg ccgttgaggc ggccgaggcc ggactggcgg tagccgcctt cctcgaattc gtcgtagacc ttcgcccaat cgttgatcca taccgtcccg gcttgcagtg cgcgcgcgac ccgcatcgag cgttgcacgt cgcgcgtcca taccgcggcg gcgaggccat attcgctgtc gttggcgagc gcgactgctt cgtcttccgt gtcgaacacc tgcagcgtca ttaccgggcc gaacgtttcg gtctgcacga tgtcgagctt cggatcgtcg acttcgagga gcgtcgggcg gtagaacgcg cctgccgcga gtgcgccgtc ggtggccggg ccgccgcgca gcaggacctt cgcgcccgcg gcgatcgcct gatcgacgct gcgatcgacg cgcgcgacgt tcgcccggtc gatgaggggc cccatttcgc tgcgcggatc ggcggcgggg ccgacgcgca ccgcgccgag tgtcgccgcg agacgcgtgc gcaccgcttc cgccacgttt cgctgcacga gcagccgcga gcccgtcatg cagaactggc cggcgaatac ggtcagcgac ttcgcgagaa ccggcagcgc ggcgtcgaga tcggcgtcgt cgaatacgac atggggtgtc ttgccgccga gctcgaggcc gaagcgcttc aggcgctgtg cgccggccgc cgagatcgcg cggccggtcg cngtcgagcc ggtgaagctg atgacgggca cgtccggaga agcgacgagg tatttcgcgc cgtcggcgcc ggattcgctg aacacgttga cgctgccgcg cggcagcgac ggcgcttccg acagcacctt ggcgacgagg gcgttcgttt gcgcggtctg gcccggcatc ttgacgaccg cgctcgcgcc ggccgcaagc gccggcgcga gcgcgcgcac catcaggacg acgggggaat tccacggcac gatgatgccg gccacgccca tcggctcccg cagcacgagc gatacgacgt ccggccgcgg cgttccgccg tgggcgcgct cggcgcgcgc gagggccgcg taatagcgca gcttcgaggg cgccatctcg acctcgaacc gggcttccgg cttgatcttg ccgttttcga gcgagagctg gtcgatgagc gcttcggtgt gccgttcgaa tgcgagtgcg agttcgtcga gagccttggc gcgcaatgcg gcgtcgtgct tccagcgcgt gcgtgcgaac gcgcgggacg ccgccgcgac ggccgcgcgc gcttcggttt cgccgccgtc ggcatagcgg ccgatcacgg cgccggtcgc cggatcgaac gaatcgcgac gagcgggcga atcgacccat tcgccgtcga tccagtgtaa agcgtgagag gc

Expected Protein Sequence

MAHHHHHHMT SASHALHWID GEWVDSPARR DSFDPATGAV IGRYADGGET EARAAVAAAS RAFARTRWKH DAALRAKALD ELALAFERHT EALIDQLSLE NGKIKPEARF EVEMAPSKLR YYAALARAER AHGGTPRPDV VSLVLREPMG VAGIIVPWNS PVVLMVRALA PALAAGASAV VKMPGQTAQT NALVAKVLSE APSLPRGSVN VFSESGADGA KYLVASPDVP VISFTGSTAT GRAISAAGAQ RLKRFGLELG GKTPHVVFDD ADLDAALPVL AKSLTVFAGQ FCMTGSRLLV QRNVAEAVRT RLAATLGAVR VGPAADPRSE MGPLIDRANV ARVDRSVDQA IAAGAKVLLR GGPATDGALA AGAFYRPTLL EVDDPKLDIV QTETFGPVMT LQVFDTEDEA VALANDSEYG LAAAVWTRDV QRSMRVARAL QAGTVWINDW AKVYDEFEEG GYRQSGLGRL NGAAAIDDFI EYKHITLSTG ASQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactt ccgcctctca cgctttacac tggatcgacg gcgaatgggt cgattcgccc gctcgtcgcg attcgttcga tccggcgacc ggcgccgtga tcggccgcta tgccgacggc ggcgaaaccg aagcgcgcgc ggccgtcgcg gcggcgtccc gcgcgttcgc acgcacgcgc tggaagcacg acgccgcatt gcgcgccaag gctctcgacg aactcgcact cgcattcgaa cggcacaccg aagcgctcat cgaccagctc tcgctcgaaa acggcaagat caagccggaa gcccggttcg aggtcgagat ggcgccctcg aagctgcgct attacgcggc cctcgcgcgc gccgagcgcg cccacggcgg aacgccgcgg ccggacgtcg tatcgctcgt gctgcgggag ccgatgggcg tggccggcat catcgtgccg tggaattccc ccgtcgtcct gatggtgcgc gcgctcgcgc cggcgcttgc ggccggcgcg agcgcggtcg tcaagatgcc gggccagacc gcgcaaacga acgccctcgt cgccaaggtg ctgtcggaag cgccgtcgct gccgcgcggc agcgtcaacg tgttcagcga atccggcgcc gacggcgcga aatacctcgt cgcttctccg gacgtgcccg tcatcagctt caccggctcg acggcgaccg gccgcgcgat ctcggcggcc ggcgcacagc gcctgaagcg cttcggcctc gagctcggcg gcaagacacc ccatgtcgta ttcgacgacg ccgatctcga cgccgcgctg ccggttctcg cgaagtcgct gaccgtattc gccggccagt tctgcatgac gggctcgcgg ctgctcgtgc agcgaaacgt ggcggaagcg gtgcgcacgc gtctcgcggc gacactcggc gcggtgcgcg tcggccccgc cgccgatccg cgcagcgaaa tggggcccct catcgaccgg gcgaacgtcg cgcgcgtcga tcgcagcgtc gatcaggcga tcgccgcggg cgcgaaggtc ctgctgcgcg gcggcccggc caccgacggc gcactcgcgg caggcgcgtt ctaccgcccg acgctcctcg aagtcgacga tccgaagctc gacatcgtgc agaccgaaac gttcggcccg gtaatgacgc tgcaggtgtt cgacacggaa gacgaagcag tcgcgctcgc caacgacagc gaatatggcc tcgccgccgc ggtatggacg cgcgacgtgc aacgctcgat gcgggtcgcg cgcgcactgc aagccgggac ggtatggatc aacgattggg cgaaggtcta cgacgaattc gaggaaggcg gctaccgcca gtccggcctc ggccgcctca acggcgccgc cgccatcgac gatttcatcg agtacaagca catcacgctg agcactgggg catcgcaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for ButhA.00020.i.B1.PS01823

PURIFICATION DATe:	5/14/2013
CONCENTRATION:	61.8mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	28
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTT CCGCCTCTCA CGCTTTACAC TGGATCGACG GCGAATGGGT CGATTCGCCC GCTCGTCGCG ATTCGTTCGA TCCGGCGACC GGCGCCGTGA TCGGCCGCTA TGCCGACGGC GGCGAAACCG AAGCGCGCGC GGCCGTCGCG GCGGCGTCCC GCGCGTTCGC ACGCACGCGC TGGAAGCACG ACGCCGCATT GCGCGCCAAG GCTCTCGACG AACTCGCACT CGCATTCGAA CGGCACACCG AAGCGCTCAT CGACCAGCTC TCGCTCGAAA ACGGCAAGAT CAAGCCGGAA GCCCGGTTCG AGGTCGAGAT GGCGCCCTCG AAGCTGCGCT ATTACGCGGC CCTCGCGCGC GCCGAGCGCG CCCACGGCGG AACGCCGCGG CCGGACGTCG TATCGCTCGT GCTGCGGGAG CCGATGGGCG TGGCCGGCAT CATCGTGCCG TGGAATTCCC CCGTCGTCCT GATGGTGCGC GCGCTCGCGC CGGCGCTTGC GGCCGGCGCG AGCGCGGTCG TCAAGATGCC GGGCCAGACC GCGCAAACGA ACGCCCTCGT CGCCAAGGTG CTGTCGGAAG CGCCGTCGCT GCCGCGCGGC AGCGTCAACG TGTTCAGCGA ATCCGGCGCC GACGGCGCGA AATACCTCGT CGCTTCTCCG GACGTGCCCG TCATCAGCTT CACCGGCTCG ACGGCGACCG GCCGCGCGAT CTCGGCGGCC GGCGCACAGC GCCTGAAGCG CTTCGGCCTC GAGCTCGGCG GCAAGACACC CCATGTCGTA TTCGACGACG CCGATCTCGA CGCCGCGCTG CCGGTTCTCG CGAAGTCGCT GACCGTATTC GCCGGCCAGT TCTGCATGAC GGGCTCGCGG CTGCTCGTGC AGCGAAACGT GGCGGAAGCG GTGCGCACGC GTCTCGCGGC GACACTCGGC GCGGTGCGCG TCGGCCCCGC CGCCGATCCG CGCAGCGAAA TGGGGCCCCT CATCGACCGG GCGAACGTCG CGCGCGTCGA TCGCAGCGTC GATCAGGCGA TCGCCGCGGG CGCGAAGGTC CTGCTGCGCG GCGGCCCGGC CACCGACGGC GCACTCGCGG CAGGCGCGTT CTACCGCCCG ACGCTCCTCG AAGTCGACGA TCCGAAGCTC GACATCGTGC AGACCGAAAC GTTCGGCCCG GTAATGACGC TGCAGGTGTT CGACACGGAA GACGAAGCAG TCGCGCTCGC CAACGACAGC GAATATGGCC TCGCCGCCGC GGTATGGACG CGCGACGTGC AACGCTCGAT GCGGGTCGCG CGCGCACTGC AAGCCGGGAC GGTATGGATC AACGATTGGG CGAAGGTCTA CGACGAATTC GAGGAAGGCG GCTACCGCCA GTCCGGCCTC GGCCGCCTCA ACGGCGCCGC CGCCATCGAC GATTTCATCG AGTACAAGCA CATCACGCTG AGCACTGGGG CATCGCAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT