ButhA.00020.h

Phenylacetaldehyde dehydrogenase

CENTER ID: ButhA.00020.h
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.00020.h.B1.GE36024 Full length( ButhA.00020.h ) 1 501
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2699
RefSeq: YP_440610.1
UniProt: Q2T2I5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLAALSTQH QRQSGFLARR QFGNWIDGAA AEPRSGRYLP VVDPATEMTI AEVAASDARD VDAAVAAARR AFDSGDWPRM RPASREKLLH QLADRIERYA DELAALETLE TGKLVGLARA IDVLGGAEYV RYMAGWATKL EGSTLDTSIA VPAGTEYFAY TRREAVGVVG AIVPWNFPLA IALWKVATAL ACGCTVVLKP SEETPLTALR LGELAQEAGL PDGVLNVVTG TGADTGAALV AHPGVDKITF TGSVGVGKAI GHAAVDRMAR FTLELGGKSP LIVFDDADPD VAARGAAQGI FFNQGQVCTA GSRVYVHKSL FERVVAGIAA AAESMKIGSG FDPNTQIGPL VSKRHFERVL GHIDAAKEEG ATLVTGGTRA LDGGYFVKPT VFVDAAPSMR IVREEVFGPV VTVTPFDTVD DAVRLANDSD FGLAASVWSQ NLSRVHRVVP RLKAGIVWVN THNMLDNNLP FGGVKQSGYG RELGRAALEQ FTELKSVCIA H
NT Sequence
ATGAATCTGG CCGCTCTCTC GACCCAGCAT CAACGCCAAT CCGGCTTTCT CGCCCGCCGC CAGTTCGGCA ACTGGATCGA CGGCGCCGCC GCCGAGCCGC GCTCGGGCCG CTATCTGCCC GTCGTCGATC CCGCGACCGA GATGACGATC GCCGAAGTCG CCGCGAGCGA CGCGCGCGAC GTCGATGCGG CCGTCGCCGC CGCGCGCCGC GCCTTCGATT CGGGCGACTG GCCGCGAATG CGGCCCGCGA GCCGCGAGAA GCTGCTGCAT CAGCTCGCCG ACCGGATCGA GCGCTACGCG GACGAGCTCG CCGCGCTCGA AACGCTCGAA ACCGGCAAGC TCGTCGGGCT CGCGCGCGCG ATCGACGTGC TGGGCGGCGC CGAATACGTG CGCTACATGG CCGGCTGGGC GACGAAGCTC GAAGGCTCGA CACTCGACAC GTCGATCGCC GTGCCCGCCG GCACGGAGTA TTTCGCGTAC ACGCGCCGCG AGGCGGTGGG CGTCGTCGGC GCGATCGTGC CGTGGAATTT TCCGCTCGCG ATCGCGTTGT GGAAGGTCGC CACCGCGCTC GCGTGCGGCT GCACGGTCGT GCTGAAGCCG TCGGAGGAAA CGCCGCTCAC CGCATTGCGC CTCGGCGAAC TCGCGCAGGA AGCAGGGCTG CCCGACGGCG TGCTGAACGT GGTGACGGGC ACGGGCGCGG ATACCGGCGC GGCGCTCGTC GCGCATCCGG GCGTCGACAA GATCACGTTC ACGGGCTCGG TCGGCGTGGG CAAGGCCATT GGACATGCGG CCGTCGACCG GATGGCGCGC TTCACGCTCG AGCTCGGCGG CAAATCGCCG CTGATCGTCT TCGACGACGC CGATCCCGAC GTCGCCGCGC GCGGCGCGGC GCAAGGCATC TTCTTCAATC AGGGGCAGGT TTGCACGGCA GGCTCGCGCG TGTACGTGCA CAAGAGCCTG TTCGAGCGGG TCGTCGCCGG CATCGCGGCG GCCGCCGAAT CGATGAAGAT CGGCTCGGGT TTCGATCCGA ACACGCAGAT CGGCCCGCTC GTGTCGAAGC GGCACTTCGA GCGCGTGCTC GGCCACATCG ACGCCGCGAA GGAAGAGGGC GCGACGCTCG TCACGGGCGG CACGCGCGCG CTCGACGGCG GCTATTTCGT GAAGCCGACG GTATTCGTCG ACGCCGCCCC CTCGATGCGG ATCGTGCGCG AAGAGGTGTT CGGCCCGGTC GTCACGGTCA CGCCGTTCGA CACGGTGGAC GACGCGGTGC GGCTCGCGAA CGACAGCGAC TTCGGGCTCG CGGCGAGCGT GTGGTCGCAG AACCTGTCGC GCGTGCATCG CGTCGTGCCG CGGCTGAAGG CGGGCATCGT CTGGGTCAAC ACGCACAACA TGCTCGACAA CAATCTGCCG TTTGGCGGCG TCAAGCAATC GGGCTACGGC CGCGAGCTCG GCCGCGCGGC GCTCGAACAG TTCACCGAGC TCAAGTCCGT CTGCATCGCG CAT
Details for ButhA.00020.h.B1.GE36024
HARVESTED ON: 10/2/2012
SEQUENCED ON: 10/1/2012
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMN LAALSTQHQR QSGFLARRQF GNWIDGAAAE PRSGRYLPVV DPATEMTIAE VAASDARDVD AAVAAARRAF DSGDWPRMRP ASREKLLHQL ADRIERYADE LAALETLETG KLVGLARAID VLGGAEYVRY MAGWATKLEG STLDTSIAVP AGTEYFAYTR REAVGVVGAI VPWNFPLAIA LWKVATALAC GCTVVLKPSX ETPLTALRLG ELAXEAXLPD GVLNVVTGTG ADTGAALVAH PGVDKITFTG SVGVGKAIGH AAVDRMARFT LELGGXSPLI VFDDXXPDVX ARXAAXXIFX XXGXXXARXX TCXXXXXXXX XXRXXXXXXX RXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
anattttntt tactttaaga aggaganata ccatggctca ccaccaccac caccatatga atctggccgc tctctcgacc cagcatcaac gccaatccgg ctttctcgcc cgccgccagt tcggcaactg gatcgacggc gccgccgccg agccgcgctc gggccgctat ctgcccgtcg tcgatcccgc gaccgagatg acgatcgccg aagtcgccgc gagcgacgcg cgcgacgtcg atgcggccgt cgccgccgcg cgccgcgcct tcgattcggg cgactggccg cgaatgcggc ccgcgagccg cgagaagctg ctgcatcagc tcgccgaccg gatcgagcgc tacgcggacg agctcgccgc gctcgaaacg ctcgaaaccg gcaagctcgt cgggctcgcg cgcgcgatcg acgtgctggg cggcgccgaa tacgtgcgct acatggccgg ctgggcgacg aagctcgaag gctcgacact cgacacgtcg atcgccgtgc ccgccggcac ggagtatttc gcgtacacgc gccgcgaggc ggtgggcgtc gtcggcgcga tcgtgccgtg gaattttccg ctcgcgatcg cgttgtggaa ggtcgccacc gcgctcgcgt gcggctgcac ggtcgtgctg aagccgtcgg angaaacgcc gctcaccgca ttgcgcctcg gcgaactcgc gcangaagca nggctgcccg acggcgtgct gaacgtggtg acgggcacgg gcgcggatac cggcgcggcg ctcgtcgcgc atccgggcgt cgacaagatc acgttcacgg gctcggtcgg cgtgggcaag gccattggac atgcggccgt cgaccggatg gcgcgcttca cgctcgagct cggcggcaan tcgccgctga tcgtcttcga cgacnncnat cccgacgtcg nngcgcgcng cgcggcgnan ngcatcttcn tcancnnngg nnnnnncnnn gctcgcgngt gnacntgcan anancngnnn ancnggnngn nnnnnnntcg nnnnnnnanc natnanatng nncgggntnn nancnnnnnn cnnannnnnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnncnnnnnn annnnnancn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttt
Expected Protein Sequence
MAHHHHHHMN LAALSTQHQR QSGFLARRQF GNWIDGAAAE PRSGRYLPVV DPATEMTIAE VAASDARDVD AAVAAARRAF DSGDWPRMRP ASREKLLHQL ADRIERYADE LAALETLETG KLVGLARAID VLGGAEYVRY MAGWATKLEG STLDTSIAVP AGTEYFAYTR REAVGVVGAI VPWNFPLAIA LWKVATALAC GCTVVLKPSE ETPLTALRLG ELAQEAGLPD GVLNVVTGTG ADTGAALVAH PGVDKITFTG SVGVGKAIGH AAVDRMARFT LELGGKSPLI VFDDADPDVA ARGAAQGIFF NQGQVCTAGS RVYVHKSLFE RVVAGIAAAA ESMKIGSGFD PNTQIGPLVS KRHFERVLGH IDAAKEEGAT LVTGGTRALD GGYFVKPTVF VDAAPSMRIV REEVFGPVVT VTPFDTVDDA VRLANDSDFG LAASVWSQNL SRVHRVVPRL KAGIVWVNTH NMLDNNLPFG GVKQSGYGRE LGRAALEQFT ELKSVCIAH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc tggccgctct ctcgacccag catcaacgcc aatccggctt tctcgcccgc cgccagttcg gcaactggat cgacggcgcc gccgccgagc cgcgctcggg ccgctatctg cccgtcgtcg atcccgcgac cgagatgacg atcgccgaag tcgccgcgag cgacgcgcgc gacgtcgatg cggccgtcgc cgccgcgcgc cgcgccttcg attcgggcga ctggccgcga atgcggcccg cgagccgcga gaagctgctg catcagctcg ccgaccggat cgagcgctac gcggacgagc tcgccgcgct cgaaacgctc gaaaccggca agctcgtcgg gctcgcgcgc gcgatcgacg tgctgggcgg cgccgaatac gtgcgctaca tggccggctg ggcgacgaag ctcgaaggct cgacactcga cacgtcgatc gccgtgcccg ccggcacgga gtatttcgcg tacacgcgcc gcgaggcggt gggcgtcgtc ggcgcgatcg tgccgtggaa ttttccgctc gcgatcgcgt tgtggaaggt cgccaccgcg ctcgcgtgcg gctgcacggt cgtgctgaag ccgtcggagg aaacgccgct caccgcattg cgcctcggcg aactcgcgca ggaagcaggg ctgcccgacg gcgtgctgaa cgtggtgacg ggcacgggcg cggataccgg cgcggcgctc gtcgcgcatc cgggcgtcga caagatcacg ttcacgggct cggtcggcgt gggcaaggcc attggacatg cggccgtcga ccggatggcg cgcttcacgc tcgagctcgg cggcaaatcg ccgctgatcg tcttcgacga cgccgatccc gacgtcgccg cgcgcggcgc ggcgcaaggc atcttcttca atcaggggca ggtttgcacg gcaggctcgc gcgtgtacgt gcacaagagc ctgttcgagc gggtcgtcgc cggcatcgcg gcggccgccg aatcgatgaa gatcggctcg ggtttcgatc cgaacacgca gatcggcccg ctcgtgtcga agcggcactt cgagcgcgtg ctcggccaca tcgacgccgc gaaggaagag ggcgcgacgc tcgtcacggg cggcacgcgc gcgctcgacg gcggctattt cgtgaagccg acggtattcg tcgacgccgc cccctcgatg cggatcgtgc gcgaagaggt gttcggcccg gtcgtcacgg tcacgccgtt cgacacggtg gacgacgcgg tgcggctcgc gaacgacagc gacttcgggc tcgcggcgag cgtgtggtcg cagaacctgt cgcgcgtgca tcgcgtcgtg ccgcggctga aggcgggcat cgtctgggtc aacacgcaca acatgctcga caacaatctg ccgtttggcg gcgtcaagca atcgggctac ggccgcgagc tcggccgcgc ggcgctcgaa cagttcaccg agctcaagtc cgtctgcatc gcgcattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t