ButhA.00020.d
N-succinylglutamate 5-semialdehyde dehydrogenase
| CENTER ID: | ButhA.00020.d |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00020.d.B1.GE36020 | Full length( ButhA.00020.d ) | 1 | 487 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4KNA
DEPOSITED: 5/9/2013
DETERMINATION: XRay
CLONE: ButhA.00020.d.B1.GE36020
PROTEIN: ButhA.00020.d.B1.PS01757
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_153480 |
| PATRIC ID: | fig|271848.6.peg.4569 |
| RefSeq: | YP_442310.1 |
| UniProt: | Q2SXN9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELFIDGAW VDGAGPVFAS RNPGTNERVW EGASASADDV ERAVASARRA FAAWSALDLD ARCTIVKRFA ALLVERKEAL ATMIGRETGK PLWEARTEVA SMAAKVDISI TAYHERTGEK RAPMADGVAV LRHRPHGVVA VFGPYNFPGH LPNGHIVPAL IAGNTVVFKP SELAPGVARA TVEIWRDAGL PAGVLNLVQG EKDTGVALAN HRQIDGLFFT GSSDTGTLLH KQFGGRPEIV LALEMGGNNP LVVAEVEDID AAVHHAIQSA FLSAGQRCTC ARRILVPRGA FGDRFVARLA DVASKITASV FDADPQPFMG AVISARAASR LVAAQARLVG LGASPIIEMK QRDPALGFVN AAILDVTNVR ELPDEEHFGP LAQIVRYTDL DDAIARANDT AFGLSAGLLA DDEQAWHTFR RAIRAGIVNW NRPTNGASSA APFGGAGRSG NHRPSAYYAA DYCAYPMASV ESAQLQMPAS LSPGLHF
NT Sequence
ATGACTGAAC TCTTCATCGA CGGCGCCTGG GTTGACGGCG CGGGCCCCGT CTTCGCTTCG CGCAACCCCG GCACGAACGA GCGCGTGTGG GAAGGCGCGA GCGCGTCGGC GGACGACGTC GAGCGCGCGG TCGCGAGCGC GCGGCGCGCG TTCGCCGCGT GGTCCGCGCT CGATCTCGAC GCGCGCTGCA CGATCGTCAA GCGCTTCGCC GCGCTTCTCG TCGAGCGCAA GGAAGCGCTC GCGACGATGA TCGGCCGCGA GACCGGCAAG CCGCTGTGGG AAGCGCGCAC CGAGGTCGCT TCGATGGCCG CGAAGGTCGA CATCTCGATC ACCGCGTATC ACGAGCGCAC CGGCGAGAAG CGCGCGCCGA TGGCCGACGG CGTCGCGGTG CTGCGGCATC GTCCGCACGG CGTCGTCGCG GTGTTCGGCC CGTACAACTT CCCGGGCCAT CTGCCGAACG GGCACATCGT GCCCGCGCTC ATCGCGGGCA ACACCGTCGT GTTCAAGCCG TCGGAGCTCG CGCCCGGCGT CGCGCGGGCC ACCGTCGAGA TCTGGCGCGA CGCGGGGCTG CCCGCGGGCG TGCTCAATCT CGTGCAGGGC GAGAAGGACA CGGGCGTCGC GCTCGCGAAC CATCGGCAGA TCGACGGTCT CTTCTTCACC GGCAGCTCGG ACACGGGCAC GCTGCTGCAC AAGCAGTTCG GCGGCCGGCC CGAGATCGTG CTCGCGCTCG AGATGGGCGG CAACAATCCG CTCGTCGTCG CCGAGGTCGA GGATATCGAC GCGGCCGTGC ATCACGCGAT CCAGTCGGCG TTCCTGTCGG CGGGGCAGCG CTGCACGTGC GCGCGCCGCA TCCTCGTGCC GCGCGGCGCG TTCGGCGACC GCTTCGTCGC GCGCCTGGCC GACGTCGCGT CGAAGATCAC GGCCAGCGTC TTCGACGCCG ATCCGCAGCC GTTCATGGGC GCGGTGATCT CCGCGCGCGC GGCGTCGCGC CTCGTCGCCG CGCAGGCGAG GCTGGTCGGA CTGGGCGCTT CGCCCATCAT CGAGATGAAG CAGCGCGATC CGGCGCTCGG CTTCGTCAAC GCGGCGATCC TCGATGTGAC GAACGTGCGC GAACTGCCGG ACGAGGAGCA CTTCGGCCCG CTCGCGCAGA TCGTTCGCTA CACCGATCTC GACGACGCGA TCGCGCGCGC GAACGACACC GCGTTCGGCC TGTCGGCGGG GCTGCTCGCC GACGACGAAC AAGCCTGGCA CACGTTCCGC CGCGCGATTC GCGCGGGCAT CGTGAACTGG AACCGGCCGA CCAACGGCGC GTCGTCGGCC GCGCCGTTCG GCGGCGCGGG CCGCTCCGGC AACCATCGCC CGAGCGCGTA CTACGCGGCC GATTACTGCG CGTATCCGAT GGCGTCGGTC GAGAGCGCGC AATTGCAGAT GCCCGCGAGC CTGTCGCCGG GCCTTCATTT C
Details for ButhA.00020.d.B1.GE36020
| HARVESTED ON: | 10/2/2012 |
| SEQUENCED ON: | 10/1/2012 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
LFIDGAWVDG AGPVFASRNP GTNERVWEGA SASADDVERA VASARRAFAA WSALDLDARC TIVKRFAALL VERKEALATM IGRETGKPLW EARTEVASMA AKVDISITAY HERTGEKRAP MADGVAVLRH RPHGVVAVFG PYNFPGHLPN GHIVPALIAG NTVVFKPSEL APGVARATVE IWRDAGLPAG VLNLVQGEKD TGVALANHRQ IDGLFFTGSS DTGTLLHKQF GGRPEIVLAL EMGGNNPLVV AEVEDIDAAV HHAIQSAFLS AGQRCTCARR ILVPRGAFGD RFVARLADVA SKITASVFDA DPQPFMGAVI SARAASRLVA AQARLVGLGA SPIIEMKQRD PALGFVNAAI LDVTNVRELP DEEHFGPLAQ IVRYTDLDDA IARANDTAFG LSAGLLADDE QAWHTFRRAI RAGIVNWNRP TNGASSAAPF GGAGRSGNHR PSAYYAADYC AYPMASVESA QLQMPA
Validated NT Sequence
cgcgggcatc tgcaattgcg cgctctcgac cgacgccatc ggatacgcgc agtaatcggc cgcgtagtac gcgctcgggc gatggttgcc ggagcggccc gcgccgccga acggcgcggc cgacgacgcg ccgttggtcg gccggttcca gttcacgatg cccgcgcgaa tcgcgcggcg gaacgtgtgc caggcttgtt cgtcgtcggc gagcagcccc gccgacaggc cgaacgcggt gtcgttcgcg cgcgcgatcg cgtcgtcgag atcggtgtag cgaacgatct gcgcgagcgg gccgaagtgc tcctcgtccg gcagttcgcg cacgttcgtc acatcgagga tcgccgcgtt gacgaagccg agcgccggat cgcgctgctt catctcgatg atgggcgaag cgcccagtcc gaccagcctc gcctgcgcgg cgacgaggcg cgacgccgcg cgcgcggaga tcaccgcgcc catgaacggc tgcggatcgg cgtcgaagac gctggccgtg atcttcgacg cgacgtcggc caggcgcgcg acgaagcggt cgccgaacgc gccgcgcggc acgaggatgc ggcgcgcgca cgtgcagcgc tgccccgccg acaggaacgc cgactggatc gcgtgatgca cggccgcgtc gatatcctcg acctcggcga cgacgagcgg attgttgccg cccatctcga gcgcgagcac gatctcgggc cggccgccga actgcttgtg cagcagcgtg cccgtgtccg agctgccggt gaagaagaga ccgtcgatct gccgatggtt cgcgagcgcg acgcccgtgt ccttctcgcc ctgcacgaga ttgagcacgc ccgcgggcag ccccgcgtcg cgccagatct cgacggtggc ccgcgcgacg ccgggcgcga gctccgacgg cttgaacacg acggtgttgc ccgcgatgag cgcgggcacg atgtgcccgt tcggcagatg gcccgggaag ttgtacgggc cgaacaccgc gacgacgccg tgcggacgat gccgcagcac cgcgacgccg tcggccatcg gcgcgcgctt ctcgccggtg cgctcgtgat acgcggtgat cgagatgtcg accttcgcgg ccatcgaagc gacctcggtg cgcgcttccc acagcggctt gccggtctcg cggccgatca tcgtcgcgag cgcttccttg cgctcgacga gaagcgcggc gaagcgcttg acgatcgtgc agcgcgcgtc gagatcgagc gcggaccacg cggcgaacgc gcgccgcgcg ctcgcgaccg cgcgctcgac gtcgtccgcc gacgcgctcg cgccttccca cacgcgctcg ttcgtgccgg ggttgcgcga agcgaagacg gggcccgcgc cgtcaaccca ggcgccgtcg atgaagag
Expected Protein Sequence
MAHHHHHHMT ELFIDGAWVD GAGPVFASRN PGTNERVWEG ASASADDVER AVASARRAFA AWSALDLDAR CTIVKRFAAL LVERKEALAT MIGRETGKPL WEARTEVASM AAKVDISITA YHERTGEKRA PMADGVAVLR HRPHGVVAVF GPYNFPGHLP NGHIVPALIA GNTVVFKPSE LAPGVARATV EIWRDAGLPA GVLNLVQGEK DTGVALANHR QIDGLFFTGS SDTGTLLHKQ FGGRPEIVLA LEMGGNNPLV VAEVEDIDAA VHHAIQSAFL SAGQRCTCAR RILVPRGAFG DRFVARLADV ASKITASVFD ADPQPFMGAV ISARAASRLV AAQARLVGLG ASPIIEMKQR DPALGFVNAA ILDVTNVREL PDEEHFGPLA QIVRYTDLDD AIARANDTAF GLSAGLLADD EQAWHTFRRA IRAGIVNWNR PTNGASSAAP FGGAGRSGNH RPSAYYAADY CAYPMASVES AQLQMPASLS PGLHF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aactcttcat cgacggcgcc tgggttgacg gcgcgggccc cgtcttcgct tcgcgcaacc ccggcacgaa cgagcgcgtg tgggaaggcg cgagcgcgtc ggcggacgac gtcgagcgcg cggtcgcgag cgcgcggcgc gcgttcgccg cgtggtccgc gctcgatctc gacgcgcgct gcacgatcgt caagcgcttc gccgcgcttc tcgtcgagcg caaggaagcg ctcgcgacga tgatcggccg cgagaccggc aagccgctgt gggaagcgcg caccgaggtc gcttcgatgg ccgcgaaggt cgacatctcg atcaccgcgt atcacgagcg caccggcgag aagcgcgcgc cgatggccga cggcgtcgcg gtgctgcggc atcgtccgca cggcgtcgtc gcggtgttcg gcccgtacaa cttcccgggc catctgccga acgggcacat cgtgcccgcg ctcatcgcgg gcaacaccgt cgtgttcaag ccgtcggagc tcgcgcccgg cgtcgcgcgg gccaccgtcg agatctggcg cgacgcgggg ctgcccgcgg gcgtgctcaa tctcgtgcag ggcgagaagg acacgggcgt cgcgctcgcg aaccatcggc agatcgacgg tctcttcttc accggcagct cggacacggg cacgctgctg cacaagcagt tcggcggccg gcccgagatc gtgctcgcgc tcgagatggg cggcaacaat ccgctcgtcg tcgccgaggt cgaggatatc gacgcggccg tgcatcacgc gatccagtcg gcgttcctgt cggcggggca gcgctgcacg tgcgcgcgcc gcatcctcgt gccgcgcggc gcgttcggcg accgcttcgt cgcgcgcctg gccgacgtcg cgtcgaagat cacggccagc gtcttcgacg ccgatccgca gccgttcatg ggcgcggtga tctccgcgcg cgcggcgtcg cgcctcgtcg ccgcgcaggc gaggctggtc ggactgggcg cttcgcccat catcgagatg aagcagcgcg atccggcgct cggcttcgtc aacgcggcga tcctcgatgt gacgaacgtg cgcgaactgc cggacgagga gcacttcggc ccgctcgcgc agatcgttcg ctacaccgat ctcgacgacg cgatcgcgcg cgcgaacgac accgcgttcg gcctgtcggc ggggctgctc gccgacgacg aacaagcctg gcacacgttc cgccgcgcga ttcgcgcggg catcgtgaac tggaaccggc cgaccaacgg cgcgtcgtcg gccgcgccgt tcggcggcgc gggccgctcc ggcaaccatc gcccgagcgc gtactacgcg gccgattact gcgcgtatcc gatggcgtcg gtcgagagcg cgcaattgca gatgcccgcg agcctgtcgc cgggccttca tttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat