SSGCID: Target Page

ButhA.00020.c

Aldehyde dehydrogenase

CENTER ID:	ButhA.00020.c
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.c.B1.GE36019	Full length( ButhA.00020.c )		1	497

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00020.c.B1.PS01724	Full length( ButhA.00020.c )		1	497

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5101
RefSeq:	YP_442792.1
UniProt:	Q2SWA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRCRRVECC AFHGDRNTTM KTCEQFYIGG AWHPSTGKGT IDVIDSGTEA VIGRIPEGTA EDAKAAVAAA RAAFDGWAAT PPAERARYLR KITDNLKARS EDLAQSITGE VGMPIKLSRA IQVGGPIYNW GAYAKLAETF AFEEPVGNSL VVREPVGVVA AITPWNYPLN QITLKVAAAL AAGCTVALKP SEIAPLNAFI LAEAIHDAEL PAGVFNLVCG YGPVVGEALA SDPEVDMVSF TGSTRAGKRV AELAAAGVKR VALELGGKSA SVILDDADFA AAVKGTVSAC YLNAGQTCSA HTRMLVPESR YEEARELAKA AAESYVAGDP RDDATRLGAL ASSAQQKRVQ DYIRRGIDEG AELVTGGLGM PEGIARGFFV KPTVFGRVKP DATIAQEEIF GPVLSIITYR DEADAVRIAN DSPYGLGGAV WSGSDERAMR IARRIRTGQV DINGGAWNMA APFGGFKQSG IGRENGVYGL EEYLEYKSMQ LRPKKGA

NT Sequence

GTGCGGCGTT GCCGGCGGGT AGAATGTTGC GCATTCCACG GAGACAGGAA CACCACGATG AAAACCTGCG AACAGTTCTA TATCGGCGGC GCGTGGCACC CGAGCACGGG CAAGGGCACG ATCGACGTGA TCGACTCGGG CACCGAGGCC GTGATCGGCC GCATTCCCGA AGGCACCGCC GAAGACGCGA AAGCGGCCGT CGCCGCCGCG CGCGCGGCGT TCGACGGCTG GGCGGCGACG CCGCCCGCCG AGCGCGCTCG CTATCTGCGC AAGATCACCG ACAACCTGAA GGCGCGCAGC GAGGACCTCG CGCAATCGAT CACGGGCGAA GTCGGCATGC CGATCAAGCT GTCGCGCGCG ATCCAGGTTG GCGGTCCGAT CTATAACTGG GGCGCGTATG CGAAGCTCGC CGAGACGTTC GCGTTCGAGG AGCCGGTCGG CAATTCGCTC GTCGTGCGCG AGCCCGTTGG TGTCGTCGCG GCGATCACGC CATGGAACTA CCCGCTCAAC CAGATCACGC TGAAGGTCGC CGCCGCACTC GCGGCGGGCT GCACGGTCGC GCTGAAGCCG TCGGAGATCG CGCCGCTCAA TGCGTTCATC CTCGCCGAGG CGATTCATGA CGCGGAGCTG CCCGCCGGCG TGTTCAATCT CGTGTGCGGT TACGGGCCCG TCGTCGGCGA GGCGCTCGCG AGCGATCCTG AGGTGGACAT GGTGTCGTTC ACCGGCTCGA CGCGCGCGGG CAAGCGCGTC GCCGAGCTCG CCGCGGCCGG CGTGAAGCGC GTCGCGCTCG AGCTGGGCGG CAAGTCGGCC TCGGTGATTC TCGACGACGC CGACTTCGCG GCCGCGGTGA AGGGCACGGT GTCCGCGTGC TACCTGAACG CGGGCCAGAC GTGCTCCGCG CATACGCGGA TGCTCGTGCC GGAATCGCGC TATGAAGAAG CACGCGAGCT CGCGAAGGCC GCCGCGGAAA GCTATGTCGC AGGCGATCCG CGCGACGACG CGACGCGGCT TGGCGCGCTC GCGTCGTCGG CTCAGCAAAA GCGCGTGCAG GATTACATCC GGCGCGGCAT CGACGAGGGC GCCGAGCTCG TCACGGGCGG CCTCGGCATG CCCGAAGGCA TCGCACGCGG CTTCTTCGTG AAGCCGACCG TGTTCGGCCG CGTGAAGCCC GACGCGACGA TCGCGCAGGA AGAAATCTTC GGTCCGGTGC TGTCGATCAT CACGTATCGC GACGAGGCCG ACGCGGTGAG GATCGCGAAC GATTCGCCGT ACGGCCTCGG CGGCGCGGTG TGGTCGGGCA GCGACGAGCG CGCGATGCGC ATCGCGCGCC GCATTCGCAC GGGGCAGGTC GACATCAACG GCGGAGCCTG GAACATGGCC GCGCCGTTCG GCGGTTTCAA ACAGTCGGGG ATCGGTCGCG AGAACGGCGT GTACGGGCTC GAAGAATATC TCGAATACAA ATCGATGCAA TTGCGGCCGA AGAAGGGCGC G

Details for ButhA.00020.c.B1.GE36019

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	53kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	83
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMV RRCRRVECCA FHGDRNTTMK TCEQFYIGGA WHPSTGKGTI DVIDSGTEAV IGRIPEGTAE DAKAAVAAAR AAFDGWAATP PAERARYLRK ITDNLKARSE DLAQSITGEV GMPIKLSRAI QVGGPIYNWG AYAKLAETFA FEEPVGNSLV VREPVGVVAA ITPWNYPLNQ ITLKVAAALA AGCTVALKPS EIAPLNAFIL AEAIHDAELP AGVFNLVCGY GPVVXEALAS DPXVDMVSFT GSTRAGKRVA ELAAAGVKRV ALELGGKSAS VXLDDADFAA AVKGTVSACY LNAGQTCSAH TXMLVPXXAX XXXXLXXXXX LCXXXXXXXX XXXXXXXQXX XXXXXXXXXX XXXXXXXXXX XXXX

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatggtg cggcgttgcc ggcgggtaga atgttgcgca ttccacggag acaggaacac cacgatgaaa acctgcgaac agttctatat cggcggcgcg tggcacccga gcacgggcaa gggcacgatc gacgtgatcg actcgggcac cgaggccgtg atcggccgca ttcccgaagg caccgccgaa gacgcgaaag cggccgtcgc cgccgcgcgc gcggcgttcg acggctgggc ggcgacgccg cccgccgagc gcgctcgcta tctgcgcaag atcaccgaca acctgaaggc gcgcagcgag gacctcgcgc aatcgatcac gggcgaagtc ggcatgccga tcaagctgtc gcgcgcgatc caggttggcg gtccgatcta taactggggc gcgtatgcga agctcgccga gacgttcgcg ttcgaggagc cggtcggcaa ttcgctcgtc gtgcgcgagc ccgttggtgt cgtcgcggcg atcacgccat ggaactaccc gctcaaccag atcacgctga aggtcgccgc cgcactcgcg gcgggctgca cggtcgcgct gaagccgtcg gagatcgcgc cgctcaatgc gttcatcctc gccgaggcga ttcatgacgc ggagctgccc gccggcgtgt tcaatctcgt gtgcggttac gggcccgtcg tcngcgaggc gctcgcgagc gatcctgang tggacatggt gtcgttcacc ggctcgacgc gcgcgggcaa gcgcgtcgcc gagctcgccg cggccggcgt gaagcgcgtc gcgctcgagc tgggcggcaa gtcggcctcg gtgantctcg acgacgccga cttcgcggcc gcggtgaagg gcacggtgtc cgcgtgctac ctgaacgcgg gccagacgtg ctccgcgcat acncnnatgc tcgtgccnnn nnncgctann nnnncncncn anctnncnnn nnnnnnnnng ctatgtancn nnnnacnacn cnacnnngnn nnnnnnnntn ncnnnnnnnn ncaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn nnnnntnnnn nng

Expected Protein Sequence

MAHHHHHHMV RRCRRVECCA FHGDRNTTMK TCEQFYIGGA WHPSTGKGTI DVIDSGTEAV IGRIPEGTAE DAKAAVAAAR AAFDGWAATP PAERARYLRK ITDNLKARSE DLAQSITGEV GMPIKLSRAI QVGGPIYNWG AYAKLAETFA FEEPVGNSLV VREPVGVVAA ITPWNYPLNQ ITLKVAAALA AGCTVALKPS EIAPLNAFIL AEAIHDAELP AGVFNLVCGY GPVVGEALAS DPEVDMVSFT GSTRAGKRVA ELAAAGVKRV ALELGGKSAS VILDDADFAA AVKGTVSACY LNAGQTCSAH TRMLVPESRY EEARELAKAA AESYVAGDPR DDATRLGALA SSAQQKRVQD YIRRGIDEGA ELVTGGLGMP EGIARGFFVK PTVFGRVKPD ATIAQEEIFG PVLSIITYRD EADAVRIAND SPYGLGGAVW SGSDERAMRI ARRIRTGQVD INGGAWNMAA PFGGFKQSGI GRENGVYGLE EYLEYKSMQL RPKKGA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc ggcgttgccg gcgggtagaa tgttgcgcat tccacggaga caggaacacc acgatgaaaa cctgcgaaca gttctatatc ggcggcgcgt ggcacccgag cacgggcaag ggcacgatcg acgtgatcga ctcgggcacc gaggccgtga tcggccgcat tcccgaaggc accgccgaag acgcgaaagc ggccgtcgcc gccgcgcgcg cggcgttcga cggctgggcg gcgacgccgc ccgccgagcg cgctcgctat ctgcgcaaga tcaccgacaa cctgaaggcg cgcagcgagg acctcgcgca atcgatcacg ggcgaagtcg gcatgccgat caagctgtcg cgcgcgatcc aggttggcgg tccgatctat aactggggcg cgtatgcgaa gctcgccgag acgttcgcgt tcgaggagcc ggtcggcaat tcgctcgtcg tgcgcgagcc cgttggtgtc gtcgcggcga tcacgccatg gaactacccg ctcaaccaga tcacgctgaa ggtcgccgcc gcactcgcgg cgggctgcac ggtcgcgctg aagccgtcgg agatcgcgcc gctcaatgcg ttcatcctcg ccgaggcgat tcatgacgcg gagctgcccg ccggcgtgtt caatctcgtg tgcggttacg ggcccgtcgt cggcgaggcg ctcgcgagcg atcctgaggt ggacatggtg tcgttcaccg gctcgacgcg cgcgggcaag cgcgtcgccg agctcgccgc ggccggcgtg aagcgcgtcg cgctcgagct gggcggcaag tcggcctcgg tgattctcga cgacgccgac ttcgcggccg cggtgaaggg cacggtgtcc gcgtgctacc tgaacgcggg ccagacgtgc tccgcgcata cgcggatgct cgtgccggaa tcgcgctatg aagaagcacg cgagctcgcg aaggccgccg cggaaagcta tgtcgcaggc gatccgcgcg acgacgcgac gcggcttggc gcgctcgcgt cgtcggctca gcaaaagcgc gtgcaggatt acatccggcg cggcatcgac gagggcgccg agctcgtcac gggcggcctc ggcatgcccg aaggcatcgc acgcggcttc ttcgtgaagc cgaccgtgtt cggccgcgtg aagcccgacg cgacgatcgc gcaggaagaa atcttcggtc cggtgctgtc gatcatcacg tatcgcgacg aggccgacgc ggtgaggatc gcgaacgatt cgccgtacgg cctcggcggc gcggtgtggt cgggcagcga cgagcgcgcg atgcgcatcg cgcgccgcat tcgcacgggg caggtcgaca tcaacggcgg agcctggaac atggccgcgc cgttcggcgg tttcaaacag tcggggatcg gtcgcgagaa cggcgtgtac gggctcgaag aatatctcga atacaaatcg atgcaattgc ggccgaagaa gggcgcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for ButhA.00020.c.B1.PS01724

PURIFICATION DATe:	1/17/2013
CONCENTRATION:	88mg/ml
OBSERVED MW:	53kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	83
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMV RRCRRVECCA FHGDRNTTMK TCEQFYIGGA WHPSTGKGTI DVIDSGTEAV IGRIPEGTAE DAKAAVAAAR AAFDGWAATP PAERARYLRK ITDNLKARSE DLAQSITGEV GMPIKLSRAI QVGGPIYNWG AYAKLAETFA FEEPVGNSLV VREPVGVVAA ITPWNYPLNQ ITLKVAAALA AGCTVALKPS EIAPLNAFIL AEAIHDAELP AGVFNLVCGY GPVVGEALAS DPEVDMVSFT GSTRAGKRVA ELAAAGVKRV ALELGGKSAS VILDDADFAA AVKGTVSACY LNAGQTCSAH TRMLVPESRY EEARELAKAA AESYVAGDPR DDATRLGALA SSAQQKRVQD YIRRGIDEGA ELVTGGLGMP EGIARGFFVK PTVFGRVKPD ATIAQEEIFG PVLSIITYRD EADAVRIAND SPYGLGGAVW SGSDERAMRI ARRIRTGQVD INGGAWNMAA PFGGFKQSGI GRENGVYGLE EYLEYKSMQL RPKKGA

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGC GGCGTTGCCG GCGGGTAGAA TGTTGCGCAT TCCACGGAGA CAGGAACACC ACGATGAAAA CCTGCGAACA GTTCTATATC GGCGGCGCGT GGCACCCGAG CACGGGCAAG GGCACGATCG ACGTGATCGA CTCGGGCACC GAGGCCGTGA TCGGCCGCAT TCCCGAAGGC ACCGCCGAAG ACGCGAAAGC GGCCGTCGCC GCCGCGCGCG CGGCGTTCGA CGGCTGGGCG GCGACGCCGC CCGCCGAGCG CGCTCGCTAT CTGCGCAAGA TCACCGACAA CCTGAAGGCG CGCAGCGAGG ACCTCGCGCA ATCGATCACG GGCGAAGTCG GCATGCCGAT CAAGCTGTCG CGCGCGATCC AGGTTGGCGG TCCGATCTAT AACTGGGGCG CGTATGCGAA GCTCGCCGAG ACGTTCGCGT TCGAGGAGCC GGTCGGCAAT TCGCTCGTCG TGCGCGAGCC CGTTGGTGTC GTCGCGGCGA TCACGCCATG GAACTACCCG CTCAACCAGA TCACGCTGAA GGTCGCCGCC GCACTCGCGG CGGGCTGCAC GGTCGCGCTG AAGCCGTCGG AGATCGCGCC GCTCAATGCG TTCATCCTCG CCGAGGCGAT TCATGACGCG GAGCTGCCCG CCGGCGTGTT CAATCTCGTG TGCGGTTACG GGCCCGTCGT CGGCGAGGCG CTCGCGAGCG ATCCTGAGGT GGACATGGTG TCGTTCACCG GCTCGACGCG CGCGGGCAAG CGCGTCGCCG AGCTCGCCGC GGCCGGCGTG AAGCGCGTCG CGCTCGAGCT GGGCGGCAAG TCGGCCTCGG TGATTCTCGA CGACGCCGAC TTCGCGGCCG CGGTGAAGGG CACGGTGTCC GCGTGCTACC TGAACGCGGG CCAGACGTGC TCCGCGCATA CGCGGATGCT CGTGCCGGAA TCGCGCTATG AAGAAGCACG CGAGCTCGCG AAGGCCGCCG CGGAAAGCTA TGTCGCAGGC GATCCGCGCG ACGACGCGAC GCGGCTTGGC GCGCTCGCGT CGTCGGCTCA GCAAAAGCGC GTGCAGGATT ACATCCGGCG CGGCATCGAC GAGGGCGCCG AGCTCGTCAC GGGCGGCCTC GGCATGCCCG AAGGCATCGC ACGCGGCTTC TTCGTGAAGC CGACCGTGTT CGGCCGCGTG AAGCCCGACG CGACGATCGC GCAGGAAGAA ATCTTCGGTC CGGTGCTGTC GATCATCACG TATCGCGACG AGGCCGACGC GGTGAGGATC GCGAACGATT CGCCGTACGG CCTCGGCGGC GCGGTGTGGT CGGGCAGCGA CGAGCGCGCG ATGCGCATCG CGCGCCGCAT TCGCACGGGG CAGGTCGACA TCAACGGCGG AGCCTGGAAC ATGGCCGCGC CGTTCGGCGG TTTCAAACAG TCGGGGATCG GTCGCGAGAA CGGCGTGTAC GGGCTCGAAG AATATCTCGA ATACAAATCG ATGCAATTGC GGCCGAAGAA GGGCGCGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT