SSGCID: Target Page

ButhA.00013.a

Holliday junction ATP-dependent DNA helicase ruvB

CENTER ID:	ButhA.00013.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00013.a.B1.GE36015	Full length( ButhA.00013.a )		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00013.a.B1.PS01788	Full length( ButhA.00013.a )		1	356

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4006
RefSeq:	YP_441794.1
UniProt:	Q2SZ55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIETDKLAAE RIIAATPASS HEEAFERALR PRQLDEYVGQ EKVRDQLEIF IEAAKRRAEA LDHVLLFGPP GLGKTTLAHI IAREMGVNLR QTSGPVLERA GDLAALLTNL EANDVLFIDE IHRLSPVVEE ILYPALEDYQ IDIMIGEGPA ARSVKLDLQP FTLVGATTRA GMLTNPLRDR FGIVARLEFY DAEQLSRIVR RSAALLNAQI DPAGALEIAK RSRGTPRIAN RLLRRVRDYA EVKADGNITA AVADAALAML DVDPVGFDLM DRKLLEAILH KFDGGPVGVD NLAAAIGEER DTIEDVLEPY LIQQGFLQRT PRGRVATLLT YRHFGLATPD AASPVRNLWD TPDAER

NT Sequence

ATGATCGAAA CCGACAAACT CGCCGCCGAG CGGATCATTG CCGCCACGCC CGCGTCTTCG CACGAAGAGG CTTTCGAACG CGCGCTGCGC CCGCGCCAGC TCGACGAATA CGTCGGCCAG GAGAAGGTGC GCGATCAGCT CGAGATCTTC ATCGAAGCCG CGAAGCGCCG CGCCGAAGCG CTCGACCACG TACTGCTGTT CGGCCCGCCC GGCCTCGGCA AGACGACGCT CGCGCACATC ATCGCGCGCG AAATGGGCGT GAACCTGCGC CAGACGTCCG GCCCCGTGCT CGAACGCGCG GGCGATCTCG CCGCGCTCCT CACGAACCTC GAAGCGAACG ACGTCCTCTT CATCGACGAG ATCCACCGCC TGTCGCCCGT CGTCGAGGAA ATCCTGTATC CGGCGCTCGA GGATTACCAG ATCGACATCA TGATCGGCGA AGGGCCGGCC GCGCGCAGCG TGAAGCTCGA CCTGCAGCCG TTCACGCTCG TCGGCGCGAC GACGCGCGCG GGCATGTTGA CCAATCCGCT GCGCGACCGC TTCGGCATCG TCGCACGGCT CGAGTTCTAC GACGCCGAAC AGCTGTCGCG CATCGTGCGC CGCTCGGCCG CGCTGCTGAA CGCTCAGATC GATCCGGCCG GCGCGCTCGA GATCGCCAAG CGCTCGCGCG GCACGCCGCG GATCGCAAAC CGCCTGCTGC GCCGCGTGCG CGACTACGCG GAAGTGAAGG CGGACGGCAA CATCACCGCG GCCGTAGCAG ACGCCGCGCT CGCAATGCTC GATGTCGATC CGGTCGGCTT CGATCTGATG GACCGCAAGC TGCTCGAAGC GATCCTGCAC AAGTTCGACG GCGGCCCGGT CGGCGTCGAC AACCTCGCCG CCGCGATCGG CGAGGAACGC GACACGATCG AGGACGTGCT CGAGCCGTAT CTGATCCAGC AGGGCTTCCT GCAACGCACG CCGCGCGGCC GCGTCGCGAC GCTCCTCACA TACCGGCATT TCGGGCTCGC CACGCCCGAC GCCGCGAGCC CCGTGCGCAA TCTCTGGGAC ACGCCCGACG CCGAGCGC

Details for ButhA.00013.a.B1.GE36015

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/1/2012
EXPECTED MW:	40kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMI ETDKLAAERI IAATPASSHE EAFERALRPR QLDEYVGQEK VRDQLEIFIE AAKRRAEALD HVLLFGPPGL GKTTLAHIIA REMGVNLRQT SGPVLERAGD LAALLTNLEA NDVLFIDEIH RLSPVVEEIL YPALEDYQID IMIGEGPAAR SVKLDLQPFT LVGATTRAGM LTNPLRDRFG IVARLEFYDA EQLSRIVRRS AALLNAQIDP AGALEIAKRS RGTPRIANRL LRRVRDYAEV KADGNITAAV ADAALAMLDV DPVGFDLMDR KLLEAILHKF XGGPVGVDNL AAAIGEERDT IEXVLEPYLI QXXXCXARRX AXXDXXXIPX IXXXXXRXXX XXXXXXXXXX PXXXXXXXXX XXXXXXXXXX XX

Validated NT Sequence

ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatgatcgaa accgacaaac tcgccgccga gcggatcatt gccgccacgc ccgcgtcttc gcacgaagag gctttcgaac gcgcgctgcg cccgcgccag ctcgacgaat acgtcggcca ggagaaggtg cgcgatcagc tcgagatctt catcgaagcc gcgaagcgcc gcgccgaagc gctcgaccac gtactgctgt tcggcccgcc cggcctcggc aagacgacgc tcgcgcacat catcgcgcgc gaaatgggcg tgaacctgcg ccagacgtcc ggccccgtgc tcgaacgcgc gggcgatctc gccgcgctcc tcacgaacct cgaagcgaac gacgtcctct tcatcgacga gatccaccgc ctgtcgcccg tcgtcgagga aatcctgtat ccggcgctcg aggattacca gatcgacatc atgatcggcg aagggccggc cgcgcgcagc gtgaagctcg acctgcagcc gttcacgctc gtcggcgcga cgacgcgcgc gggcatgttg accaatccgc tgcgcgaccg cttcggcatc gtcgcacggc tcgagttcta cgacgccgaa cagctgtcgc gcatcgtgcg ccgctcggcc gcgctgctga acgctcagat cgatccggcc ggcgcgctcg agatcgccaa gcgctcgcgc ggcacgccgc ggatcgcaaa ccgcctgctg cgccgcgtgc gcgactacgc ggaagtgaag gcggacggca acatcaccgc ggccgtagca gacgccgcgc tcgcaatgct cgatgtcgat ccggtcggct tcgatctgat ggaccgcaag ctgctcgaag cgatcctgca caagttcgan ggcggcccgg tcggcgtcga caacctcgcc gccgcgatcg gngaggaacg cgacacgatc gagnacgtgc tcgagccgta tctgatccag cnnnnntnct gcnacgcacg ccgnnnngcn nnnnncgacn cncntcncat accngnnatt ngggntngnc nnnnccgann ncnnnnnncc nnnnnnannn nnnnnnnnnc ccnnnnnccn nnnncnnann nnnnnanann ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnan nnnt

Expected Protein Sequence

MAHHHHHHMI ETDKLAAERI IAATPASSHE EAFERALRPR QLDEYVGQEK VRDQLEIFIE AAKRRAEALD HVLLFGPPGL GKTTLAHIIA REMGVNLRQT SGPVLERAGD LAALLTNLEA NDVLFIDEIH RLSPVVEEIL YPALEDYQID IMIGEGPAAR SVKLDLQPFT LVGATTRAGM LTNPLRDRFG IVARLEFYDA EQLSRIVRRS AALLNAQIDP AGALEIAKRS RGTPRIANRL LRRVRDYAEV KADGNITAAV ADAALAMLDV DPVGFDLMDR KLLEAILHKF DGGPVGVDNL AAAIGEERDT IEDVLEPYLI QQGFLQRTPR GRVATLLTYR HFGLATPDAA SPVRNLWDTP DAER

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg aaaccgacaa actcgccgcc gagcggatca ttgccgccac gcccgcgtct tcgcacgaag aggctttcga acgcgcgctg cgcccgcgcc agctcgacga atacgtcggc caggagaagg tgcgcgatca gctcgagatc ttcatcgaag ccgcgaagcg ccgcgccgaa gcgctcgacc acgtactgct gttcggcccg cccggcctcg gcaagacgac gctcgcgcac atcatcgcgc gcgaaatggg cgtgaacctg cgccagacgt ccggccccgt gctcgaacgc gcgggcgatc tcgccgcgct cctcacgaac ctcgaagcga acgacgtcct cttcatcgac gagatccacc gcctgtcgcc cgtcgtcgag gaaatcctgt atccggcgct cgaggattac cagatcgaca tcatgatcgg cgaagggccg gccgcgcgca gcgtgaagct cgacctgcag ccgttcacgc tcgtcggcgc gacgacgcgc gcgggcatgt tgaccaatcc gctgcgcgac cgcttcggca tcgtcgcacg gctcgagttc tacgacgccg aacagctgtc gcgcatcgtg cgccgctcgg ccgcgctgct gaacgctcag atcgatccgg ccggcgcgct cgagatcgcc aagcgctcgc gcggcacgcc gcggatcgca aaccgcctgc tgcgccgcgt gcgcgactac gcggaagtga aggcggacgg caacatcacc gcggccgtag cagacgccgc gctcgcaatg ctcgatgtcg atccggtcgg cttcgatctg atggaccgca agctgctcga agcgatcctg cacaagttcg acggcggccc ggtcggcgtc gacaacctcg ccgccgcgat cggcgaggaa cgcgacacga tcgaggacgt gctcgagccg tatctgatcc agcagggctt cctgcaacgc acgccgcgcg gccgcgtcgc gacgctcctc acataccggc atttcgggct cgccacgccc gacgccgcga gccccgtgcg caatctctgg gacacgcccg acgccgagcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for ButhA.00013.a.B1.PS01788

PURIFICATION DATe:	3/14/2013
CONCENTRATION:	49.7mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	88
PERCENT COVERAGE:	104

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMI ETDKLAAERI IAATPASSHE EAFERALRPR QLDEYVGQEK VRDQLEIFIE AAKRRAEALD HVLLFGPPGL GKTTLAHIIA REMGVNLRQT SGPVLERAGD LAALLTNLEA NDVLFIDEIH RLSPVVEEIL YPALEDYQID IMIGEGPAAR SVKLDLQPFT LVGATTRAGM LTNPLRDRFG IVARLEFYDA EQLSRIVRRS AALLNAQIDP AGALEIAKRS RGTPRIANRL LRRVRDYAEV KADGNITAAV ADAALAMLDV DPVGFDLMDR KLLEAILHKF DGGPVGVDNL AAAIGEERDT IEDVLEPYLI QQGFLQRTPR GRVATLLTYR HFGLATPDAA SPVRNLWDTP DAER

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG AAACCGACAA ACTCGCCGCC GAGCGGATCA TTGCCGCCAC GCCCGCGTCT TCGCACGAAG AGGCTTTCGA ACGCGCGCTG CGCCCGCGCC AGCTCGACGA ATACGTCGGC CAGGAGAAGG TGCGCGATCA GCTCGAGATC TTCATCGAAG CCGCGAAGCG CCGCGCCGAA GCGCTCGACC ACGTACTGCT GTTCGGCCCG CCCGGCCTCG GCAAGACGAC GCTCGCGCAC ATCATCGCGC GCGAAATGGG CGTGAACCTG CGCCAGACGT CCGGCCCCGT GCTCGAACGC GCGGGCGATC TCGCCGCGCT CCTCACGAAC CTCGAAGCGA ACGACGTCCT CTTCATCGAC GAGATCCACC GCCTGTCGCC CGTCGTCGAG GAAATCCTGT ATCCGGCGCT CGAGGATTAC CAGATCGACA TCATGATCGG CGAAGGGCCG GCCGCGCGCA GCGTGAAGCT CGACCTGCAG CCGTTCACGC TCGTCGGCGC GACGACGCGC GCGGGCATGT TGACCAATCC GCTGCGCGAC CGCTTCGGCA TCGTCGCACG GCTCGAGTTC TACGACGCCG AACAGCTGTC GCGCATCGTG CGCCGCTCGG CCGCGCTGCT GAACGCTCAG ATCGATCCGG CCGGCGCGCT CGAGATCGCC AAGCGCTCGC GCGGCACGCC GCGGATCGCA AACCGCCTGC TGCGCCGCGT GCGCGACTAC GCGGAAGTGA AGGCGGACGG CAACATCACC GCGGCCGTAG CAGACGCCGC GCTCGCAATG CTCGATGTCG ATCCGGTCGG CTTCGATCTG ATGGACCGCA AGCTGCTCGA AGCGATCCTG CACAAGTTCG ACGGCGGCCC GGTCGGCGTC GACAACCTCG CCGCCGCGAT CGGCGAGGAA CGCGACACGA TCGAGGACGT GCTCGAGCCG TATCTGATCC AGCAGGGCTT CCTGCAACGC ACGCCGCGCG GCCGCGTCGC GACGCTCCTC ACATACCGGC ATTTCGGGCT CGCCACGCCC GACGCCGCGA GCCCCGTGCG CAATCTCTGG GACACGCCCG ACGCCGAGCG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT