ButhA.00010.z
Oxidoreductase, short chain dehydrogenase/reductase family
| CENTER ID: | ButhA.00010.z |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00010.z.B1.GE39397 | Full length( ButhA.00010.z ) | 1 | 255 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00010.z.B1.PS02557 | Full length( ButhA.00010.z ) | 1 | 255 |
Structures
5TT0
DEPOSITED: 10/31/2016
DETERMINATION: XRay
CLONE: ButhA.00010.z.B1.GE39397
PROTEIN: ButhA.00010.z.B1.PS02557
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|271848.6.peg.1325 |
| RefSeq: | YP_439391.1 |
| UniProt: | Q2T608 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTPRTTLIT GAAGGIGQAL VRRFLAAGDR VLALDRDRAA LAAFVDALGG AAVAPVVDDL TDAARLADAL ANERVDVLVA NAGTAASATL RATTSASWRA DLDANLTATY VSVEAVLAGM RARRRGAITI VGSVNGVAAL GHPAYSAAKA GLISYAKSLA IEYGRDGVRA NVVCPGTVKT PAWEARVRQN PQVFEQLKKW YPLDDFATPD DVANAALFLS SDAARAITGA MLPVDGGLLA GNRVMAQELT LESFY
NT Sequence
ATGACGACAC CACGCACCAC GCTGATCACG GGCGCCGCGG GCGGCATCGG CCAGGCGCTC GTGCGCCGCT TTCTTGCCGC GGGCGACCGT GTGCTCGCGC TCGACCGCGA CCGCGCGGCG CTCGCCGCGT TCGTCGATGC GCTCGGCGGC GCGGCCGTCG CGCCCGTCGT CGACGACCTG ACCGACGCCG CGCGCCTCGC GGACGCGCTC GCGAACGAGC GCGTCGACGT GCTCGTCGCG AACGCGGGCA CCGCCGCATC GGCGACGCTG CGCGCGACGA CGAGCGCGTC GTGGCGCGCG GATCTGGACG CGAACCTGAC CGCGACCTAT GTGAGCGTCG AGGCCGTGCT CGCCGGCATG CGCGCGCGCC GCCGCGGCGC GATCACGATC GTCGGCTCGG TCAACGGCGT CGCCGCGCTC GGCCACCCCG CCTATAGCGC GGCGAAGGCG GGACTCATCA GTTATGCGAA ATCGCTCGCG ATCGAATACG GGCGCGACGG CGTGCGCGCG AACGTCGTGT GCCCGGGCAC CGTGAAGACG CCCGCGTGGG AAGCGCGCGT GCGGCAAAAC CCGCAGGTGT TCGAGCAATT GAAGAAGTGG TATCCGCTCG ACGATTTCGC GACGCCCGAC GATGTCGCGA ACGCCGCGCT GTTCCTCTCG TCGGACGCGG CGCGCGCGAT CACCGGCGCG ATGCTGCCCG TCGACGGCGG GCTGCTCGCC GGCAACCGCG TGATGGCGCA GGAGCTGACG CTCGAGTCGT TCTAT
Details for ButhA.00010.z.B1.GE39397
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT TPRTTLITGA AGGIGQALVR RFLAAGDRVL ALDRDRAALA AFVDALGGAA VAPVVDDLTD AARLADALAN ERVDVLVANA GTAASATLRA TTSASWRADL DANLTATYVS VEAVLAGMRA RRRGAITIVG SVNGVAALGH PAYSAAKAGL ISYAKSLAIE YGRDGVRANV VCPGTVKTPA WEARVRQNPQ VFEQLKKWYP LDDFATPDDV ANAALFLSSD AARAITGAML PVDGGLLAGN RVMAXXXXLE SFY
Validated NT Sequence
atcttactca cttaatagaa cgactcgagc nnnnnnnnnt gcgccatcac gcggttgccg gcgagcagcc cgccgtcgac gggcagcatc gcgccggtga tcgcgcgcgc cgcgtccgac gagaggaaca gcgcggcgtt cgcgacatcg tcgggcgtcg cgaaatcgtc gagcggatac cacttcttca attgctcgaa cacctgcggg ttttgccgca cgcgcgcttc ccacgcgggc gtcttcacgg tgcccgggca cacgacgttc gcgcgcacgc cgtcgcgccc gtattcgatc gcgagcgatt tcgcataact gatgagtccc gccttcgccg cgctataggc ggggtggccg agcgcggcga cgccgttgac cgagccgacg atcgtgatcg cgccgcggcg gcgcgcgcgc atgccggcga gcacggcctc gacgctcaca taggtcgcgg tcaggttcgc gtccagatcc gcgcgccacg acgcgctcgt cgtcgcgcgc agcgtcgccg atgcggcggt gcccgcgttc gcgacgagca cgtcgacgcg ctcgttcgcg agcgcgtccg cgaggcgcgc ggcgtcggtc aggtcgtcga cgacgggcgc gacggccgcg ccgccgagcg catcgacgaa cgcggcgagc gccgcgcggt cgcggtcgag cgcgagcaca cggtcgcccg cggcaagaaa gcggcgcacg agcgcctggc cgatgccgcc cgcggcgccc gtgatcagcg tggtgcgtgg tgtcgtcata tggtggtggt ggtggtgagc catggt
Expected Protein Sequence
MAHHHHHHMT TPRTTLITGA AGGIGQALVR RFLAAGDRVL ALDRDRAALA AFVDALGGAA VAPVVDDLTD AARLADALAN ERVDVLVANA GTAASATLRA TTSASWRADL DANLTATYVS VEAVLAGMRA RRRGAITIVG SVNGVAALGH PAYSAAKAGL ISYAKSLAIE YGRDGVRANV VCPGTVKTPA WEARVRQNPQ VFEQLKKWYP LDDFATPDDV ANAALFLSSD AARAITGAML PVDGGLLAGN RVMAQELTLE SFY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga caccacgcac cacgctgatc acgggcgccg cgggcggcat cggccaggcg ctcgtgcgcc gctttcttgc cgcgggcgac cgtgtgctcg cgctcgaccg cgaccgcgcg gcgctcgccg cgttcgtcga tgcgctcggc ggcgcggccg tcgcgcccgt cgtcgacgac ctgaccgacg ccgcgcgcct cgcggacgcg ctcgcgaacg agcgcgtcga cgtgctcgtc gcgaacgcgg gcaccgccgc atcggcgacg ctgcgcgcga cgacgagcgc gtcgtggcgc gcggatctgg acgcgaacct gaccgcgacc tatgtgagcg tcgaggccgt gctcgccggc atgcgcgcgc gccgccgcgg cgcgatcacg atcgtcggct cggtcaacgg cgtcgccgcg ctcggccacc ccgcctatag cgcggcgaag gcgggactca tcagttatgc gaaatcgctc gcgatcgaat acgggcgcga cggcgtgcgc gcgaacgtcg tgtgcccggg caccgtgaag acgcccgcgt gggaagcgcg cgtgcggcaa aacccgcagg tgttcgagca attgaagaag tggtatccgc tcgacgattt cgcgacgccc gacgatgtcg cgaacgccgc gctgttcctc tcgtcggacg cggcgcgcgc gatcaccggc gcgatgctgc ccgtcgacgg cgggctgctc gccggcaacc gcgtgatggc gcaggagctg acgctcgagt cgttctattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ButhA.00010.z.B1.PS02557
| PURIFICATION DATe: | 12/16/2015 |
| CONCENTRATION: | 3.46mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TPRTTLITGA AGGIGQALVR RFLAAGDRVL ALDRDRAALA AFVDALGGAA VAPVVDDLTD AARLADALAN ERVDVLVANA GTAASATLRA TTSASWRADL DANLTATYVS VEAVLAGMRA RRRGAITIVG SVNGVAALGH PAYSAAKAGL ISYAKSLAIE YGRDGVRANV VCPGTVKTPA WEARVRQNPQ VFEQLKKWYP LDDFATPDDV ANAALFLSSD AARAITGAML PVDGGLLAGN RVMAXXXXLE SFY
Validated NT Sequence
atcttactca cttaatagaa cgactcgagc nnnnnnnnnt gcgccatcac gcggttgccg gcgagcagcc cgccgtcgac gggcagcatc gcgccggtga tcgcgcgcgc cgcgtccgac gagaggaaca gcgcggcgtt cgcgacatcg tcgggcgtcg cgaaatcgtc gagcggatac cacttcttca attgctcgaa cacctgcggg ttttgccgca cgcgcgcttc ccacgcgggc gtcttcacgg tgcccgggca cacgacgttc gcgcgcacgc cgtcgcgccc gtattcgatc gcgagcgatt tcgcataact gatgagtccc gccttcgccg cgctataggc ggggtggccg agcgcggcga cgccgttgac cgagccgacg atcgtgatcg cgccgcggcg gcgcgcgcgc atgccggcga gcacggcctc gacgctcaca taggtcgcgg tcaggttcgc gtccagatcc gcgcgccacg acgcgctcgt cgtcgcgcgc agcgtcgccg atgcggcggt gcccgcgttc gcgacgagca cgtcgacgcg ctcgttcgcg agcgcgtccg cgaggcgcgc ggcgtcggtc aggtcgtcga cgacgggcgc gacggccgcg ccgccgagcg catcgacgaa cgcggcgagc gccgcgcggt cgcggtcgag cgcgagcaca cggtcgcccg cggcaagaaa gcggcgcacg agcgcctggc cgatgccgcc cgcggcgccc gtgatcagcg tggtgcgtgg tgtcgtcata tggtggtggt ggtggtgagc catggt
Expressed Protein Sequence
MAHHHHHHMT TPRTTLITGA AGGIGQALVR RFLAAGDRVL ALDRDRAALA AFVDALGGAA VAPVVDDLTD AARLADALAN ERVDVLVANA GTAASATLRA TTSASWRADL DANLTATYVS VEAVLAGMRA RRRGAITIVG SVNGVAALGH PAYSAAKAGL ISYAKSLAIE YGRDGVRANV VCPGTVKTPA WEARVRQNPQ VFEQLKKWYP LDDFATPDDV ANAALFLSSD AARAITGAML PVDGGLLAGN RVMAQELTLE SFY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CACCACGCAC CACGCTGATC ACGGGCGCCG CGGGCGGCAT CGGCCAGGCG CTCGTGCGCC GCTTTCTTGC CGCGGGCGAC CGTGTGCTCG CGCTCGACCG CGACCGCGCG GCGCTCGCCG CGTTCGTCGA TGCGCTCGGC GGCGCGGCCG TCGCGCCCGT CGTCGACGAC CTGACCGACG CCGCGCGCCT CGCGGACGCG CTCGCGAACG AGCGCGTCGA CGTGCTCGTC GCGAACGCGG GCACCGCCGC ATCGGCGACG CTGCGCGCGA CGACGAGCGC GTCGTGGCGC GCGGATCTGG ACGCGAACCT GACCGCGACC TATGTGAGCG TCGAGGCCGT GCTCGCCGGC ATGCGCGCGC GCCGCCGCGG CGCGATCACG ATCGTCGGCT CGGTCAACGG CGTCGCCGCG CTCGGCCACC CCGCCTATAG CGCGGCGAAG GCGGGACTCA TCAGTTATGC GAAATCGCTC GCGATCGAAT ACGGGCGCGA CGGCGTGCGC GCGAACGTCG TGTGCCCGGG CACCGTGAAG ACGCCCGCGT GGGAAGCGCG CGTGCGGCAA AACCCGCAGG TGTTCGAGCA ATTGAAGAAG TGGTATCCGC TCGACGATTT CGCGACGCCC GACGATGTCG CGAACGCCGC GCTGTTCCTC TCGTCGGACG CGGCGCGCGC GATCACCGGC GCGATGCTGC CCGTCGACGG CGGGCTGCTC GCCGGCAACC GCGTGATGGC GCAGGAGCTG ACGCTCGAGT CGTTCTATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT