SSGCID: Target Page

ButhA.00010.v

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	ButhA.00010.v
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.v.B1.GE39396	Full length( ButhA.00010.v )		1	278

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.v.B1.PS37861	Full length( ButhA.00010.v )		1	278

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.1723
RefSeq:	YP_439757.1
UniProt:	Q2T4Z2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNISRFPRLA RATDDIGFAD MTVDTVSSSS IPLAWIAGVG ASAGLGAAVG RRFARAGYGV VLTGRTHERV AAAADEIRAQ GGVAYVASGD IASEADVERL AIEVRTLGTL RAAVFNAGNA VRAPTLELSV AQFESAWRTN VIGGFLFAKA ALEQLLDAGD GAPGDETPRS LLFTGATASL RGRPPFAAFA SAKAGLRSLA QTLAREFGPR GIHVAHAVID GGIDGERLRG AAPERAAQAG AAGLLSPDAI ADSYWQLHVQ HRSAWTQELD LRPCDEAF

NT Sequence

ATGAACATAT CTCGTTTTCC TCGGTTGGCA CGCGCGACAG ACGATATAGG GTTCGCCGAC ATGACGGTCG ATACGGTTTC ATCCAGTTCC ATTCCGCTCG CTTGGATCGC GGGCGTCGGC GCGAGCGCCG GGCTCGGCGC GGCCGTCGGC CGGCGCTTCG CGCGCGCCGG CTACGGCGTC GTGCTGACCG GGCGCACGCA CGAGCGCGTC GCCGCGGCCG CCGACGAGAT TCGCGCGCAG GGCGGCGTCG CATACGTCGC GAGCGGCGAC ATCGCGAGCG AAGCCGACGT CGAGCGCCTC GCGATCGAGG TGCGCACGCT CGGCACGCTG CGCGCCGCGG TGTTCAACGC GGGCAACGCG GTTCGCGCGC CGACGCTCGA GCTGAGCGTC GCGCAGTTCG AGAGCGCGTG GCGCACGAAC GTGATCGGCG GCTTCCTGTT CGCGAAGGCG GCGCTCGAGC AACTGCTCGA CGCGGGCGAT GGCGCGCCGG GCGACGAAAC GCCCCGCAGC CTGCTGTTCA CGGGCGCGAC CGCGTCGCTG CGCGGCCGCC CGCCGTTCGC CGCGTTCGCA TCGGCGAAGG CGGGCCTGCG CTCGCTCGCG CAGACGCTCG CGCGCGAGTT CGGCCCGCGC GGCATTCACG TCGCGCACGC GGTGATCGAC GGCGGCATCG ACGGCGAGCG GCTGCGTGGC GCGGCGCCCG AGCGCGCCGC GCAGGCGGGC GCCGCCGGGC TGCTGTCGCC CGACGCGATC GCCGACAGCT ACTGGCAACT GCATGTGCAG CACCGCAGTG CATGGACACA GGAGCTCGAT CTGCGCCCAT GCGACGAAGC GTTC

Details for ButhA.00010.v.B1.GE39396

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	30kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMN ISRFPRLARA TDDIGFADMT VDTVSSSSIP LAWIAGVGAS AGLGAAVGRR FARAGYGVVL TGRTHERVAA AADEIRAQGG VAYVASXXXX SEADVERLAI EVRTLXXLRA AVFNAGNAVR APTLELSVAQ FESAWRTNVI GGFLFAKAAL EQLLDAGDGA PXDETPRSXL FTGXXASLRX RPPFXAFASA KAGLRSLAQT LAREFGPRGI HVAHAVIDGG IDGERLRGAA PERAAQAGAA GLLSPDAIAD SYWQLHVQHR SAWTQE

Validated NT Sequence

agctcctgtg tccatgcact gcggtgctgc acatgcagtt gccagtagct gtcggcgatc gcgtcgggcg acagcagccc ggcggcgccc gcctgcgcgg cgcgctcggg cgccgcgcca cgcagccgct cgccgtcgat gccgccgtcg atcaccgcgt gcgcgacgtg aatgccgcgc gggccgaact cgcgcgcgag cgtctgcgcg agcgagcgca ggcccgcctt cgccgatgcg aacgcgncga acggcgggcg nnngcgcagc gacgcgnnnn cgcccgtgaa cagcnggctg cggggcgttt cgtcnnccgg cgcgccatcg cccgcgtcga gcagttgctc gagcgccgcc ttcgcgaaca ggaagccgcc gatcacgttc gtgcgccacg cgctctcgaa ctgcgcgacg ctcagctcga gcgtcggcgc gcgaaccgcg ttgcccgcgt tgaacaccgc ggcgcgcagc gnnncgagcg tgcgcacctc gatcgcgagg cgctcgacgt cggcttcgct cnnnatgtnn ncgctcgcga cgtatgcgac gccgccctgc gcgcgaatct cgtcggcggc cgcggcgacg cgctcgtgcg tgcgcccggt cagcacgacg ccgtagccgg cgcgcgcgaa gcgccggccg acggccgcgc cgagcccggc gctcgcgccg acgcccgcga tccaagcgag cggaatggaa ctggatgaaa ccgtatcgac cgtcatgtcg gcgaacccta tatcgtctgt cgcgcgtgcc aaccgaggaa aacgagatat gttcatatgg tggtggtggt ggtgagccat ggt

Expected Protein Sequence

MAHHHHHHMN ISRFPRLARA TDDIGFADMT VDTVSSSSIP LAWIAGVGAS AGLGAAVGRR FARAGYGVVL TGRTHERVAA AADEIRAQGG VAYVASGDIA SEADVERLAI EVRTLGTLRA AVFNAGNAVR APTLELSVAQ FESAWRTNVI GGFLFAKAAL EQLLDAGDGA PGDETPRSLL FTGATASLRG RPPFAAFASA KAGLRSLAQT LAREFGPRGI HVAHAVIDGG IDGERLRGAA PERAAQAGAA GLLSPDAIAD SYWQLHVQHR SAWTQELDLR PCDEAF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca tatctcgttt tcctcggttg gcacgcgcga cagacgatat agggttcgcc gacatgacgg tcgatacggt ttcatccagt tccattccgc tcgcttggat cgcgggcgtc ggcgcgagcg ccgggctcgg cgcggccgtc ggccggcgct tcgcgcgcgc cggctacggc gtcgtgctga ccgggcgcac gcacgagcgc gtcgccgcgg ccgccgacga gattcgcgcg cagggcggcg tcgcatacgt cgcgagcggc gacatcgcga gcgaagccga cgtcgagcgc ctcgcgatcg aggtgcgcac gctcggcacg ctgcgcgccg cggtgttcaa cgcgggcaac gcggttcgcg cgccgacgct cgagctgagc gtcgcgcagt tcgagagcgc gtggcgcacg aacgtgatcg gcggcttcct gttcgcgaag gcggcgctcg agcaactgct cgacgcgggc gatggcgcgc cgggcgacga aacgccccgc agcctgctgt tcacgggcgc gaccgcgtcg ctgcgcggcc gcccgccgtt cgccgcgttc gcatcggcga aggcgggcct gcgctcgctc gcgcagacgc tcgcgcgcga gttcggcccg cgcggcattc acgtcgcgca cgcggtgatc gacggcggca tcgacggcga gcggctgcgt ggcgcggcgc ccgagcgcgc cgcgcaggcg ggcgccgccg ggctgctgtc gcccgacgcg atcgccgaca gctactggca actgcatgtg cagcaccgca gtgcatggac acaggagctc gatctgcgcc catgcgacga agcgttctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for ButhA.00010.v.B1.PS37861

PURIFICATION DATe:	3/2/2016
CONCENTRATION:	22mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	95
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA TATCTCGTTT TCCTCGGTTG GCACGCGCGA CAGACGATAT AGGGTTCGCC GACATGACGG TCGATACGGT TTCATCCAGT TCCATTCCGC TCGCTTGGAT CGCGGGCGTC GGCGCGAGCG CCGGGCTCGG CGCGGCCGTC GGCCGGCGCT TCGCGCGCGC CGGCTACGGC GTCGTGCTGA CCGGGCGCAC GCACGAGCGC GTCGCCGCGG CCGCCGACGA GATTCGCGCG CAGGGCGGCG TCGCATACGT CGCGAGCGGC GACATCGCGA GCGAAGCCGA CGTCGAGCGC CTCGCGATCG AGGTGCGCAC GCTCGGCACG CTGCGCGCCG CGGTGTTCAA CGCGGGCAAC GCGGTTCGCG CGCCGACGCT CGAGCTGAGC GTCGCGCAGT TCGAGAGCGC GTGGCGCACG AACGTGATCG GCGGCTTCCT GTTCGCGAAG GCGGCGCTCG AGCAACTGCT CGACGCGGGC GATGGCGCGC CGGGCGACGA AACGCCCCGC AGCCTGCTGT TCACGGGCGC GACCGCGTCG CTGCGCGGCC GCCCGCCGTT CGCCGCGTTC GCATCGGCGA AGGCGGGCCT GCGCTCGCTC GCGCAGACGC TCGCGCGCGA GTTCGGCCCG CGCGGCATTC ACGTCGCGCA CGCGGTGATC GACGGCGGCA TCGACGGCGA GCGGCTGCGT GGCGCGGCGC CCGAGCGCGC CGCGCAGGCG GGCGCCGCCG GGCTGCTGTC GCCCGACGCG ATCGCCGACA GCTACTGGCA ACTGCATGTG CAGCACCGCA GTGCATGGAC ACAGGAGCTC GATCTGCGCC CATGCGACGA AGCGTTCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT