SSGCID: Target Page

ButhA.00010.n

Oxidoreductase, short-chain dehydrogenase/reductase family

CENTER ID:	ButhA.00010.n
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.n.B2.GE39395	Mature protein( ButhA.00010.n )		23	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3019
RefSeq:	YP_440910.1
UniProt:	Q2T1N9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAPPPLKVF ITGASSGLGL ALAAEYARRG ATLGLVARRA DALAEFAQRF PKATISIHPA DVRDADALAL AASRFVAAHG CPDVVIANAG ISKGAITGEG DLDAFREIMD VNYYGMIATF EPFIAPMTAA RRGTLVGIAS VAGVRGLPGS GAYSASKAAA IKYLEALRVE LRPAQVAVVT IAPGYIRTPM TEHNPYPMPF LMDADRFAKR AALAISQKAA FRVIPWQMGV VAKLLHVMPR WLYDRLFEKA PRKPRAKAG

NT Sequence

ATGAACGCCC CTCCTCCGCT CAAGGTCTTC ATCACCGGCG CGTCCAGCGG GCTCGGGCTC GCGCTCGCCG CCGAATATGC GCGGCGCGGC GCGACGCTCG GCCTCGTCGC CCGGCGCGCC GACGCGCTCG CCGAGTTCGC GCAACGCTTC CCCAAGGCCA CGATCTCGAT CCACCCGGCC GACGTGCGCG ACGCCGATGC GCTCGCGCTC GCCGCGTCGC GCTTCGTTGC CGCGCACGGG TGCCCCGACG TCGTGATCGC GAACGCCGGC ATCAGCAAGG GCGCGATCAC GGGCGAAGGC GACCTCGACG CGTTCCGCGA GATCATGGAT GTCAACTACT ACGGGATGAT CGCGACGTTC GAGCCGTTCA TCGCGCCGAT GACGGCCGCG CGGCGCGGCA CGCTCGTCGG CATCGCGAGC GTCGCCGGCG TGCGCGGGCT GCCGGGCTCG GGTGCGTACA GCGCGTCGAA GGCCGCGGCG ATCAAGTATC TCGAGGCGCT GAGGGTCGAG CTGCGGCCCG CGCAGGTTGC GGTCGTGACG ATCGCGCCCG GCTACATTCG CACGCCGATG ACCGAGCACA ACCCGTATCC GATGCCGTTC CTGATGGATG CGGACCGCTT CGCGAAGCGC GCGGCGCTCG CGATCTCGCA GAAAGCCGCG TTTCGCGTGA TTCCGTGGCA GATGGGCGTC GTCGCGAAGC TGCTGCACGT AATGCCGCGC TGGCTGTACG ATCGCCTGTT CGAGAAGGCG CCCCGCAAGC CCAGGGCGAA GGCGGGC

Details for ButhA.00010.n.B2.GE39395

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA AEYARRGATL GLVARRADAL AEFAQRFPKA TISIHPADVR DADALALAAS RFVAAHGCPD VVIANAGISK GAITGEGDLD AFREIMDVNY YGMIATFEPF IAPMTAARRG TLVGIASVAG VRGLPGSGAY SASKAAAIKY LEALRVELRP AQVAVVTIAP GYIRTPMTEH NPYPMPFLMD ADRFAKRAAL AISQKAAFRV IPWQMGVVAK LLHVMPRWLY DRLFEKAPXX XRAKAG

Validated NT Sequence

atcttactca cttagcccgc cttcgccctg gnnnnnnngg gcgccttctc gaacaggcga tcgtacagcc agcgcggcat tacgtgcagc agcttcgcga cgacgcccat ctgccacgga atcacgcgaa acgcggcttt ctgcgagatc gcgagcgccg cgcgcttcgc gaagcggtcc gcatccatca ggaacggcat cggatacggg ttgtgctcgg tcatcggcgt gcgaatgtag ccgggcgcga tcgtcacgac cgcaacctgc gcgggccgca gctcgaccct cagcgcctcg agatacttga tcgccgcggc cttcgacgcg ctgtacgcac ccgagcccgg cagcccgcgc acgccggcga cgctcgcgat gccgacgagc gtgccgcgcc gcgcggccgt catcggcgcg atgaacggct cgaacgtcgc gatcatcccg tagtagttga catccatgat ctcgcggaac gcgtcgaggt cgccttcgcc cgtgatcgcg cccttgctga tgccggcgtt cgcgatcacg acgtcggggc acccgtgcgc ggcaacgaag cgcgacgcgg cgagcgcgag cgcatcggcg tcgcgcacgt cggccgggtg gatcgagatc gtggccttgg ggaagcgttg cgcgaactcg gcgagcgcgt cggcgcgccg ggcgacgagg ccgagcgtcg cgccgcgccg cgcatattcg gcggccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHAA EYARRGATLG LVARRADALA EFAQRFPKAT ISIHPADVRD ADALALAASR FVAAHGCPDV VIANAGISKG AITGEGDLDA FREIMDVNYY GMIATFEPFI APMTAARRGT LVGIASVAGV RGLPGSGAYS ASKAAAIKYL EALRVELRPA QVAVVTIAPG YIRTPMTEHN PYPMPFLMDA DRFAKRAALA ISQKAAFRVI PWQMGVVAKL LHVMPRWLYD RLFEKAPRKP RAKAG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgccg aatatgcgcg gcgcggcgcg acgctcggcc tcgtcgcccg gcgcgccgac gcgctcgccg agttcgcgca acgcttcccc aaggccacga tctcgatcca cccggccgac gtgcgcgacg ccgatgcgct cgcgctcgcc gcgtcgcgct tcgttgccgc gcacgggtgc cccgacgtcg tgatcgcgaa cgccggcatc agcaagggcg cgatcacggg cgaaggcgac ctcgacgcgt tccgcgagat catggatgtc aactactacg ggatgatcgc gacgttcgag ccgttcatcg cgccgatgac ggccgcgcgg cgcggcacgc tcgtcggcat cgcgagcgtc gccggcgtgc gcgggctgcc gggctcgggt gcgtacagcg cgtcgaaggc cgcggcgatc aagtatctcg aggcgctgag ggtcgagctg cggcccgcgc aggttgcggt cgtgacgatc gcgcccggct acattcgcac gccgatgacc gagcacaacc cgtatccgat gccgttcctg atggatgcgg accgcttcgc gaagcgcgcg gcgctcgcga tctcgcagaa agccgcgttt cgcgtgattc cgtggcagat gggcgtcgtc gcgaagctgc tgcacgtaat gccgcgctgg ctgtacgatc gcctgttcga gaaggcgccc cgcaagccca gggcgaaggc gggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat