ButhA.00010.l
3-hydroxyacyl-CoA dehydrogenase
| CENTER ID: | ButhA.00010.l |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.00010.l.B1.GE36013 | Full length( ButhA.00010.l ) | 1 | 252 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4XGN
DEPOSITED: 12/31/2014
DETERMINATION: XRay
CLONE: ButhA.00010.l.B1.GE36013
PROTEIN: ButhA.00010.l.B1.PS01726
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129031 |
| PATRIC ID: | fig|271848.6.peg.3448 |
| RefSeq: | YP_441294.1 |
| UniProt: | Q2T0K5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEIRDNVFLI TGGASGLGAG TARLLTEAGG KVVLADLNQD AGEALARELG GVFVRCDVAR EEDAQAAVAA ATKLGTLRGL VNCAGIAPAA KTVGKDGPHP LELFAKTITV NLIGTFNMIR VAAAAMAANE PAPTGERGVI VSTASVAAFD GQIGQAAYAA SKAGVAGMTL PIARDLSRNA IRVMTIAPGI FETPMLLGMP QEVQDALGAM VPFPPRLGKP AEYAMLVRQI FENPMLNGEV IRLDGAIRMQ PK
NT Sequence
ATGGAGATTC GCGACAACGT CTTCCTGATC ACGGGCGGCG CATCCGGGCT CGGAGCGGGC ACCGCGCGCC TGCTGACCGA AGCAGGCGGC AAGGTCGTGC TCGCGGACCT GAATCAGGAC GCGGGCGAAG CGCTCGCGCG CGAGCTGGGC GGCGTGTTCG TCCGGTGCGA CGTCGCGCGC GAGGAAGACG CGCAGGCGGC CGTCGCCGCC GCGACGAAGC TCGGCACGCT GCGCGGGCTC GTCAACTGCG CGGGCATCGC GCCCGCGGCG AAAACGGTCG GCAAGGACGG CCCGCACCCG CTCGAGCTGT TCGCGAAGAC GATCACCGTG AACCTGATCG GCACGTTCAA CATGATTCGC GTCGCGGCCG CTGCGATGGC CGCGAACGAG CCGGCGCCAA CCGGCGAGCG CGGCGTGATC GTCAGCACCG CGTCGGTCGC CGCGTTCGAC GGCCAGATCG GCCAGGCCGC GTACGCGGCG TCGAAGGCGG GCGTCGCCGG CATGACGCTG CCCATCGCGC GCGACCTGTC GCGCAATGCG ATTCGCGTGA TGACGATCGC GCCCGGCATC TTCGAAACGC CGATGCTGCT CGGCATGCCG CAGGAAGTGC AGGACGCGCT CGGCGCGATG GTGCCGTTCC CGCCGCGCCT CGGCAAGCCG GCCGAATACG CGATGCTCGT CAGGCAGATC TTCGAGAACC CGATGCTCAA CGGCGAGGTC ATCCGCCTAG ACGGCGCGAT TCGGATGCAG CCGAAG
Details for ButhA.00010.l.B1.GE36013
| HARVESTED ON: | 10/2/2012 |
| SEQUENCED ON: | 10/1/2012 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM EIRDNVFLIT GGASGLGAGT ARLLTEAGGK VVLADLNQDA GEALARELGG VFVRCDVARE EDAQAAVAAA TKLGTLRGLV NCAGIAPAAK TVGKDGPHPL ELFAKTITVN LIGTFNMIRV AAAAMAANEP APTGERGVIV STASVAAFDG QIGQAAYAAS KAGVAGMTLP IARDLSRNAI RVMTIAPGIF ETPMLLGMPX EVQDALGAMV PFPPRLGKPA EYAMLVRQIF ENPMLNGEVI RLDGAIRMQP K
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccaccatatg gagattcgcg acaacgtctt cctgatcacg ggcggcgcat ccgggctcgg agcgggcacc gcgcgcctgc tgaccgaagc aggcggcaag gtcgtgctcg cggacctgaa tcaggacgcg ggcgaagcgc tcgcgcgcga gctgggcggc gtgttcgtcc ggtgcgacgt cgcgcgcgag gaagacgcgc aggcggccgt cgccgccgcg acgaagctcg gcacgctgcg cgggctcgtc aactgcgcgg gcatcgcgcc cgcggcgaaa acggtcggca aggacggccc gcacccgctc gagctgttcg cgaagacgat caccgtgaac ctgatcggca cgttcaacat gattcgcgtc gcggccgctg cgatggccgc gaacgagccg gcgccaaccg gcgagcgcgg cgtgatcgtc agcaccgcgt cggtcgccgc gttcgacggc cagatcggcc aggccgcgta cgcggcgtcg aaggcgggcg tcgccggcat gacgctgccc atcgcgcgcg acctgtcgcg caatgcgatt cgcgtgatga cgatcgcgcc cggcatcttc gaaacgccga tgctgctcgg catgccgcan gaagtgcagg acgcgctcgg cgcgatggtg ccgttcccgc cgcgcctcgg caagccggcc gaatacgcga tgctcgtcag gcagatcttc gagaacccga tgctcaacgg cgaggtcatc cgcctagacg gcgcgattcg gatgcagccg aagtaagtga gtaagatagg atccggnnnn nnnnannccn nnanna
Expected Protein Sequence
MAHHHHHHME IRDNVFLITG GASGLGAGTA RLLTEAGGKV VLADLNQDAG EALARELGGV FVRCDVAREE DAQAAVAAAT KLGTLRGLVN CAGIAPAAKT VGKDGPHPLE LFAKTITVNL IGTFNMIRVA AAAMAANEPA PTGERGVIVS TASVAAFDGQ IGQAAYAASK AGVAGMTLPI ARDLSRNAIR VMTIAPGIFE TPMLLGMPQE VQDALGAMVP FPPRLGKPAE YAMLVRQIFE NPMLNGEVIR LDGAIRMQPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga ttcgcgacaa cgtcttcctg atcacgggcg gcgcatccgg gctcggagcg ggcaccgcgc gcctgctgac cgaagcaggc ggcaaggtcg tgctcgcgga cctgaatcag gacgcgggcg aagcgctcgc gcgcgagctg ggcggcgtgt tcgtccggtg cgacgtcgcg cgcgaggaag acgcgcaggc ggccgtcgcc gccgcgacga agctcggcac gctgcgcggg ctcgtcaact gcgcgggcat cgcgcccgcg gcgaaaacgg tcggcaagga cggcccgcac ccgctcgagc tgttcgcgaa gacgatcacc gtgaacctga tcggcacgtt caacatgatt cgcgtcgcgg ccgctgcgat ggccgcgaac gagccggcgc caaccggcga gcgcggcgtg atcgtcagca ccgcgtcggt cgccgcgttc gacggccaga tcggccaggc cgcgtacgcg gcgtcgaagg cgggcgtcgc cggcatgacg ctgcccatcg cgcgcgacct gtcgcgcaat gcgattcgcg tgatgacgat cgcgcccggc atcttcgaaa cgccgatgct gctcggcatg ccgcaggaag tgcaggacgc gctcggcgcg atggtgccgt tcccgccgcg cctcggcaag ccggccgaat acgcgatgct cgtcaggcag atcttcgaga acccgatgct caacggcgag gtcatccgcc tagacggcgc gattcggatg cagccgaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat