SSGCID: Target Page

ButhA.00010.i

Glucose 1-dehydrogenase

CENTER ID:	ButhA.00010.i
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.00010.i.B1.GE36010	Full length( ButhA.00010.i )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3794
RefSeq:	YP_441589.1
UniProt:	Q2SZR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPGSRSKLM TNPPAKAVLI TGASRGIGRA TALLAAAHGW SVGINYARDA AAAEATADAV RAAGAQACIV RGDVANETDV IAMFDAVQSA FGRLDALVNN AGIVAPSLPL ADMDVARLKR VFDTNVLGAY LCAREAARRL STDRGGMGGA IANVSSIAAR LGSPNEYVDY AGSKGAVDTL TLGLAKELGP RGVRVNAVRP GLIATEIHAS GGQPGRAERL GAQTPLGRAG EADEVAEAIV WLLSDAASYV TGALLDVGGG R

NT Sequence

GTGCCGCCCG GGTCGAGGTC CAAGCTGATG ACGAACCCGC CAGCCAAAGC CGTCCTCATC ACCGGCGCGA GCCGCGGCAT CGGCCGCGCA ACCGCGCTTC TCGCCGCCGC GCACGGCTGG TCGGTCGGCA TCAACTACGC GCGCGACGCG GCAGCCGCCG AAGCAACCGC CGACGCGGTG CGCGCCGCCG GCGCGCAAGC GTGCATCGTG CGCGGCGACG TCGCGAACGA AACCGACGTG ATCGCCATGT TCGACGCGGT GCAGTCCGCG TTCGGCCGGC TCGACGCGCT CGTCAACAAC GCGGGAATCG TCGCGCCGTC GCTGCCGCTC GCCGACATGG ACGTCGCACG CCTGAAGCGC GTATTCGACA CCAACGTGCT CGGCGCGTAT CTGTGCGCGC GCGAAGCCGC GCGGCGGCTG TCGACCGATC GCGGCGGCAT GGGCGGCGCG ATCGCCAACG TCTCGTCGAT CGCCGCGCGG CTCGGCTCGC CGAACGAGTA CGTCGACTAT GCGGGTTCGA AGGGCGCGGT CGACACGCTG ACGCTCGGCC TCGCGAAGGA GCTCGGCCCG CGCGGCGTAC GCGTGAATGC GGTTCGCCCG GGCCTCATCG CGACCGAGAT CCATGCGAGC GGCGGCCAGC CCGGCCGTGC CGAGCGACTC GGCGCGCAGA CGCCGCTCGG CCGCGCAGGC GAAGCAGACG AGGTGGCCGA GGCGATCGTC TGGCTGCTGA GCGACGCCGC GTCGTACGTG ACGGGCGCGC TGCTCGACGT CGGCGGCGGG CGC

Details for ButhA.00010.i.B1.GE36010

HARVESTED ON:	10/2/2012
SEQUENCED ON:	10/2/2012
EXPECTED MW:	27kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV PPGSRSKLMT NPPAKAVLIT GASRGIGRAT ALLAAAHGWS VGINYARDAA AAEATADAVR AAGAQACIVR GDVANETDVI AMFDAVQSAF GRLDALVNNA GIVAPSLPLA DMDVARLKRV FDTNVLGAYL CAREAARRLS TDRGGMGGAI ANVSSIAARL GSPNEYVDYA GSKGAVDTLT LGLAKELGPR GVRVNAVRPG LIATEIHASG GQPGRAERLG AQTPLGRAGE XXEXAXXIVW LLSDAASYVT GALLDVXGGR

Validated NT Sequence

atggctcacc accaccacca ccatatggtg ccgcccgggt cgaggtccaa gctgatgacg aacccgccag ccaaagccgt cctcatcacc ggcgcgagcc gcggcatcgg ccgcgcaacc gcgcttctcg ccgccgcgca cggctggtcg gtcggcatca actacgcgcg cgacgcggca gccgccgaag caaccgccga cgcggtgcgc gccgccggcg cgcaagcgtg catcgtgcgc ggcgacgtcg cgaacgaaac cgacgtgatc gccatgttcg acgcggtgca gtccgcgttc ggccggctcg acgcgctcgt caacaacgcg ggaatcgtcg cgccgtcgct gccgctcgcc gacatggacg tcgcacgcct gaagcgcgta ttcgacacca acgtgctcgg cgcgtatctg tgcgcgcgcg aagccgcgcg gcggctgtcg accgatcgcg gcggcatggg cggcgcgatc gccaacgtct cgtcgatcgc cgcgcggctc ggctcgccga acgagtacgt cgactatgcg ggttcgaagg gcgcggtcga cacgctgacg ctcggcctcg cgaaggagct cggcccgcgc ggcgtacgcg tgaatgcggt tcgcccgggc ctcatcgcga ccgagatcca tgcgagcggc ggccagcccg gccgtgccga gcgactcggc gcgcagacgc cgctcggccg cgcaggcgaa ncagangagn tggccgannc gatcgtctgg ctgctgagcg acgccgcgtc gtacgtgacg ggcgcgctgc tcgacgtcng cggcgggcgc taagtgagta a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc cgcccgggtc gaggtccaag ctgatgacga acccgccagc caaagccgtc ctcatcaccg gcgcgagccg cggcatcggc cgcgcaaccg cgcttctcgc cgccgcgcac ggctggtcgg tcggcatcaa ctacgcgcgc gacgcggcag ccgccgaagc aaccgccgac gcggtgcgcg ccgccggcgc gcaagcgtgc atcgtgcgcg gcgacgtcgc gaacgaaacc gacgtgatcg ccatgttcga cgcggtgcag tccgcgttcg gccggctcga cgcgctcgtc aacaacgcgg gaatcgtcgc gccgtcgctg ccgctcgccg acatggacgt cgcacgcctg aagcgcgtat tcgacaccaa cgtgctcggc gcgtatctgt gcgcgcgcga agccgcgcgg cggctgtcga ccgatcgcgg cggcatgggc ggcgcgatcg ccaacgtctc gtcgatcgcc gcgcggctcg gctcgccgaa cgagtacgtc gactatgcgg gttcgaaggg cgcggtcgac acgctgacgc tcggcctcgc gaaggagctc ggcccgcgcg gcgtacgcgt gaatgcggtt cgcccgggcc tcatcgcgac cgagatccat gcgagcggcg gccagcccgg ccgtgccgag cgactcggcg cgcagacgcc gctcggccgc gcaggcgaag cagacgaggt ggccgaggcg atcgtctggc tgctgagcga cgccgcgtcg tacgtgacgg gcgcgctgct cgacgtcggc ggcgggcgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat